Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 119,536 G→T 100% P11P (CCG→CCT)  aceE → pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273119,5360GT98.4% 779.8 / 2.9 250P11P (CCG→CCT) aceEpyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (172/73);  total (174/76)
Fisher's exact test for biased strand distribution p-value = 8.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 250 total aligned reads displayed.

ATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAC  >  CP009273/119393‑119680
                                                                                                                                               |                                                                                                                                                
  aaaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaa                                                                                                                                            >  1:1783378/1‑148 (MQ=255)
   aaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaaa                                                                                                                                           <  1:870575/148‑1 (MQ=255)
   aaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaaa                                                                                                                                           <  2:2347426/148‑1 (MQ=255)
   aaaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGaaa                                                                                                                                           <  1:2917084/148‑1 (MQ=255)
    aaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAAc                                                                                                                                          >  2:1738343/1‑148 (MQ=255)
    aaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAAc                                                                                                                                          >  1:3719715/1‑148 (MQ=255)
    aaGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAAc                                                                                                                                          >  1:3009689/1‑148 (MQ=255)
     aGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACt                                                                                                                                         >  1:2084587/1‑148 (MQ=255)
      gcgcGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTc                                                                                                                                        >  2:1341219/1‑148 (MQ=255)
      gcgcGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTc                                                                                                                                        >  2:1386993/1‑148 (MQ=255)
              aCTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTgg                                                                                                                                >  2:2737537/1‑148 (MQ=255)
                tAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCt                                                                                                                              <  2:2300602/148‑1 (MQ=255)
                    cGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAg                                                                                                                          <  2:1321188/148‑1 (MQ=255)
                         aaCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGAt                                                                                                                     >  2:612849/1‑148 (MQ=255)
                          aCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATc                                                                                                                    >  2:3486653/1‑148 (MQ=255)
                          aCCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATc                                                                                                                    >  1:2435060/1‑148 (MQ=255)
                             tGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGaa                                                                                                                 >  1:1238312/1‑148 (MQ=255)
                             tGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGaa                                                                                                                 <  1:1807830/148‑1 (MQ=255)
                             tGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGaa                                                                                                                 >  1:2572110/1‑148 (MQ=255)
                              gTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAAt                                                                                                                <  1:1243411/148‑1 (MQ=255)
                               tCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATc                                                                                                               >  2:3125324/1‑148 (MQ=255)
                                  tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                                                                                            >  2:2022325/1‑148 (MQ=255)
                                  tATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGt                                                                                                            >  2:2023048/1‑148 (MQ=255)
                                    ttGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCa                                                                                                          >  1:1090619/1‑148 (MQ=255)
                                     tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCAt                                                                                                         >  2:158642/1‑148 (MQ=255)
                                     tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCAt                                                                                                         <  2:3154125/148‑1 (MQ=255)
                                     tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCAt                                                                                                         <  1:632327/148‑1 (MQ=255)
                                     tGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCAt                                                                                                         <  2:1826025/148‑1 (MQ=255)
                                           ttCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACTTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTga                                                                                                   <  1:728382/148‑1 (MQ=255)
                                              cGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTgaaga                                                                                                <  2:1433175/148‑1 (MQ=255)
                                                     agTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGtt                                                                                         >  1:3331636/1‑148 (MQ=255)
                                                      gTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTg                                                                                        >  1:3694047/1‑148 (MQ=255)
                                                      gTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTg                                                                                        <  1:1013257/148‑1 (MQ=255)
                                                          aaTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCg                                                                                    <  1:2635404/148‑1 (MQ=255)
                                                          aaTGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCg                                                                                    <  2:1088516/148‑1 (MQ=255)
                                                            tGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTg                                                                                  <  1:2675089/148‑1 (MQ=255)
                                                             gggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGc                                                                                 <  1:2944086/148‑1 (MQ=255)
                                                              ggACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCt                                                                                >  2:2951192/1‑148 (MQ=255)
                                                               gACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTc                                                                               >  1:1640934/1‑148 (MQ=255)
                                                                   ggTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTa                                                                           >  1:1561775/1‑148 (MQ=255)
                                                                    gTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTAt                                                                          >  1:343894/1‑148 (MQ=255)
                                                                        cAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGa                                                                      >  2:2437980/1‑148 (MQ=255)
                                                                         aGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGAt                                                                     >  1:2334169/1‑148 (MQ=255)
                                                                          gAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATc                                                                    >  1:2872270/1‑148 (MQ=255)
                                                                          gAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATc                                                                    >  2:1799475/1‑148 (MQ=255)
                                                                              aCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGAcc                                                                >  2:1509051/1‑148 (MQ=255)
                                                                              aCTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGAcc                                                                >  2:215598/1‑148 (MQ=255)
                                                                               cTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCa                                                               >  2:1926203/1‑148 (MQ=255)
                                                                                tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTTAGCGTGCTCAGTATCTGATCGACCaa                                                              <  2:3513444/148‑1 (MQ=255)
                                                                                tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCaa                                                              <  2:1525706/148‑1 (MQ=255)
                                                                                tCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCaa                                                              >  1:2432819/1‑148 (MQ=255)
                                                                                 cAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAAc                                                             >  1:2168288/1‑148 (MQ=255)
                                                                                  aaCGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACt                                                            >  2:399597/1‑148 (MQ=255)
                                                                                     gTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgct                                                         <  2:95700/148‑1 (MQ=255)
                                                                                        attaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgc                                                      >  2:961461/1‑148 (MQ=255)
                                                                                         ttaGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACtgcttgct                                                     >  1:2829313/1‑148 (MQ=255)
                                                                                            gatagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGaa                                                  >  1:3344648/1‑148 (MQ=255)
                                                                                             atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAg                                                 >  2:1347085/1‑148 (MQ=255)
                                                                                             atagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAg                                                 >  2:1900752/1‑148 (MQ=255)
                                                                                              tagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc                                                >  1:763110/1‑148 (MQ=255)
                                                                                              tagataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGc                                                >  1:3112623/1‑148 (MQ=255)
                                                                                               agataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGcc                                               >  2:1346635/1‑148 (MQ=255)
                                                                                               agataAGGAATAACCCATGTCAGAACGTTTCCAAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACAAACTGCTTGCTGAAGcc                                               >  1:2893574/1‑148 (MQ=255)
                                                                                                gataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGccc                                              >  2:3775715/1‑148 (MQ=255)
                                                                                                 ataAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCg                                             >  1:194865/1‑148 (MQ=255)
                                                                                                      gAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAg                                        >  2:876573/1‑148 (MQ=255)
                                                                                                      gAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAg                                        >  2:2793081/1‑148 (MQ=255)
                                                                                                       aaTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAgg                                       >  1:3196116/1‑148 (MQ=255)
                                                                                                        aTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGc                                      <  2:1083693/148‑1 (MQ=255)
                                                                                                        aTAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGc                                      >  1:957922/1‑148 (MQ=255)
                                                                                                            cccATGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACAAACTGCTTGCTGAAGCCCGCAAAGGCGgtg                                  >  1:2581443/1‑148 (MQ=255)
                                                                                                               aTGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaa                               >  2:1637325/1‑148 (MQ=255)
                                                                                                               aTGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaa                               <  2:2572110/148‑1 (MQ=255)
                                                                                                                tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                              >  1:457827/1‑148 (MQ=255)
                                                                                                                tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                              >  2:1007189/1‑148 (MQ=255)
                                                                                                                tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                              >  1:702114/1‑148 (MQ=255)
                                                                                                                tGTCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTaaa                              <  1:612849/148‑1 (MQ=255)
                                                                                                                  tCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACg                            >  1:3748083/1‑148 (MQ=255)
                                                                                                                  tCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACg                            >  1:2719746/1‑148 (MQ=255)
                                                                                                                  tCAGAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACg                            <  2:3230734/148‑1 (MQ=255)
                                                                                                                     gAACGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAg                         >  1:1372501/1‑148 (MQ=255)
                                                                                                                      aaCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAATCCCGCAAAGGCGGTGTAAACGTAGc                        >  2:3796863/1‑148 (MQ=255)
                                                                                                                      aaCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGc                        >  2:28772/1‑148 (MQ=255)
                                                                                                                       aCGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGcc                       >  1:557920/1‑148 (MQ=255)
                                                                                                                        cGTTTCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCg                      <  2:702114/148‑1 (MQ=255)
                                                                                                                          tttCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCa                    <  1:2783910/148‑1 (MQ=255)
                                                                                                                           ttCCCAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAg                   >  1:939808/1‑148 (MQ=255)
                                                                                                                             cccAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGc                 >  2:1197482/1‑148 (MQ=255)
                                                                                                                               cAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGcac               >  2:3265371/1‑148 (MQ=255)
                                                                                                                               cAAATGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGcac               >  2:2116588/1‑148 (MQ=255)
                                                                                                                                aaaTGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGcaca              >  2:3008144/1‑148 (MQ=255)
                                                                                                                                   tGACGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGt           <  1:3547844/148‑1 (MQ=255)
                                                                                                                                     aCGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTAt         <  1:3460874/148‑1 (MQ=255)
                                                                                                                                     aCGTGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTAt         <  1:1560795/148‑1 (MQ=255)
                                                                                                                                        tGGATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAg      >  2:1445760/1‑148 (MQ=255)
                                                                                                                                          gATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCa    >  1:135101/1‑148 (MQ=255)
                                                                                                                                          gATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCa    >  1:1566226/1‑148 (MQ=255)
                                                                                                                                          gATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCa    >  2:3711179/1‑148 (MQ=255)
                                                                                                                                          gATCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCa    >  2:3633006/1‑148 (MQ=255)
                                                                                                                                            tCCTATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAc  >  2:244820/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
ATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAACGTTATTAGATAGATAAGGAATAACCCATGTCAGAACGTTTCCCAAATGACGTGGATCCGATCGAAACTCGCGACTGGCTCCAGGCGATCGAATCGGTCATCCGTGAAGAAGGTGTTGAGCGTGCTCAGTATCTGATCGACCAACTGCTTGCTGAAGCCCGCAAAGGCGGTGTAAACGTAGCCGCAGGCACAGGTATCAGCAAC  >  CP009273/119393‑119680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑