Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,531,029 G→T 100% R180S (CGC→AGC)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,531,0290GT100.0% 737.9 / ‑6.7 238R180S (CGC→AGC) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (123/113);  total (125/113)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

CCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAT  >  CP009273/1530884‑1531173
                                                                                                                                                 |                                                                                                                                                
 ccGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGgt                                                                                                                                               <  1:3011155/148‑1 (MQ=255)
  cGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAATCGCCTGGCTGgtg                                                                                                                                              >  1:3616262/1‑148 (MQ=255)
   gCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGgtgt                                                                                                                                             <  2:2213232/148‑1 (MQ=255)
    cACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTc                                                                                                                                            >  1:3193039/1‑148 (MQ=255)
    cACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTc                                                                                                                                            <  2:2711071/148‑1 (MQ=255)
    cACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTc                                                                                                                                            >  1:59442/1‑148 (MQ=255)
     aCTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCa                                                                                                                                           >  1:1184414/1‑148 (MQ=255)
          tGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTTATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTTGTGTCATcgcg                                                                                                                                      <  2:3203002/148‑1 (MQ=255)
             cGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctc                                                                                                                                   <  1:2596301/148‑1 (MQ=255)
             cGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctc                                                                                                                                   <  2:1383996/148‑1 (MQ=255)
             cGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctc                                                                                                                                   >  1:2964655/1‑148 (MQ=255)
             cGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctc                                                                                                                                   <  2:131398/148‑1 (MQ=255)
              gACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCGTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGctct                                                                                                                                  <  2:3272145/148‑1 (MQ=255)
                          cgcgACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACt                                                                                                                      <  1:1056026/148‑1 (MQ=255)
                              aCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtt                                                                                                                  <  2:1218049/148‑1 (MQ=255)
                                aTCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGtttt                                                                                                                >  1:3452610/1‑148 (MQ=255)
                                    tGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGa                                                                                                            >  2:3151887/1‑148 (MQ=255)
                                     ggCTAAAGCGTCGCTGATATTGATTAATCGGCGAGCTGTCACACGCCGTGGCGTTATATTCAATAAACTTCACCAGTTCCTCTTCCCACACAGCATCAAGCGCCTGGCTGGTGTCAACGCGCTCTTCGCTGTTTAATTGTTTTGTGac                                                                                                           >  1:452760/1‑148 (MQ=255)
                                       cTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGGTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac                                                                                                         <  2:1957308/148‑1 (MQ=255)
                                         aaaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                       >  2:1355342/1‑148 (MQ=255)
                                         aaaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                       >  2:890811/1‑148 (MQ=255)
                                         aaaGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTg                                                                                                       >  2:308239/1‑148 (MQ=255)
                                           aGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCGGTTCCTCTTCCCACACAGCAACAAGCGCCGGGCGGGTGTCATCGCGCTCTTCGCTGGTTACTTGTTTTGTGACACTGTc                                                                                                     >  1:986784/1‑148 (MQ=255)
                                               tCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACtt                                                                                                 >  2:3554017/1‑148 (MQ=255)
                                               tCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACtt                                                                                                 >  1:2796981/1‑148 (MQ=255)
                                                  cTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaa                                                                                              <  2:2651640/148‑1 (MQ=255)
                                                  cTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGaa                                                                                              <  1:2485248/148‑1 (MQ=255)
                                                     atatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAgg                                                                                           <  1:2806360/148‑1 (MQ=255)
                                                      tatTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAggg                                                                                          >  1:1836375/1‑148 (MQ=255)
                                                       atTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGa                                                                                         >  2:1120505/1‑148 (MQ=255)
                                                       atTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGa                                                                                         >  1:1019539/1‑148 (MQ=255)
                                                        ttGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAg                                                                                        >  1:2940626/1‑148 (MQ=255)
                                                        ttGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAg                                                                                        <  1:3161899/148‑1 (MQ=255)
                                                         tGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGc                                                                                       <  1:2138952/148‑1 (MQ=255)
                                                           aTTAAGCGGCGAGCAGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCATCTTCCCACACAGAATCAATAGCCTGGCTGGTGTAATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACAGTCACTTTAAAGGGAGCtt                                                                                     >  1:1298060/1‑148 (MQ=255)
                                                            ttAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTc                                                                                    >  2:2851251/1‑148 (MQ=255)
                                                             tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                   >  1:3379025/1‑148 (MQ=255)
                                                             tAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTcc                                                                                   <  2:3453034/148‑1 (MQ=255)
                                                                gcggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGc                                                                                >  1:1753818/1‑148 (MQ=255)
                                                                gcggcgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGGTCCTCTTCCCACACTGCATCAATCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGc                                                                                <  2:1041390/148‑1 (MQ=255)
                                                                    cgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGTTTCCCGCCAGt                                                                            <  1:1355342/148‑1 (MQ=255)
                                                                    cgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGt                                                                            <  1:1824270/148‑1 (MQ=255)
                                                                      aGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTg                                                                          >  2:3704226/1‑148 (MQ=255)
                                                                       gCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgc                                                                         <  2:1863437/148‑1 (MQ=255)
                                                                       gCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgc                                                                         >  1:176894/1‑148 (MQ=255)
                                                                        cTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgca                                                                        >  2:2106498/1‑148 (MQ=255)
                                                                        cTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgca                                                                        >  1:2649917/1‑148 (MQ=255)
                                                                        cTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgca                                                                        >  1:731720/1‑148 (MQ=255)
                                                                          gTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagc                                                                      >  1:1782378/1‑148 (MQ=255)
                                                                           tCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca                                                                     >  2:3124606/1‑148 (MQ=255)
                                                                            cacaCGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGcagcag                                                                    <  1:1453083/148‑1 (MQ=255)
                                                                              cacGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCagag                                                                  >  2:1011126/1‑148 (MQ=255)
                                                                               acGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGc                                                                 <  1:523184/148‑1 (MQ=255)
                                                                               acGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGc                                                                 <  1:3151887/148‑1 (MQ=255)
                                                                                cGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCa                                                                <  1:3700051/148‑1 (MQ=255)
                                                                                 gCCGTGGCGTTATCTTCAATAAACTTCACCTGGTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCAt                                                               <  2:2584039/148‑1 (MQ=255)
                                                                                     tGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCaaa                                                           >  1:646857/1‑148 (MQ=255)
                                                                                      ggCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAaac                                                          >  2:1441756/1‑148 (MQ=255)
                                                                                          ttATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcg                                                      <  2:1942025/148‑1 (MQ=255)
                                                                                          ttATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcg                                                      <  1:2106498/148‑1 (MQ=255)
                                                                                           tATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAcgc                                                     >  1:193154/1‑148 (MQ=255)
                                                                                                tCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTAcc                                                >  2:462835/1‑148 (MQ=255)
                                                                                                 cAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCa                                               <  2:1829306/148‑1 (MQ=255)
                                                                                                   aTAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACg                                             >  1:2794711/1‑148 (MQ=255)
                                                                                                      aaCTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTg                                          >  2:1731248/1‑148 (MQ=255)
                                                                                                       aCTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGc                                         <  1:1120505/148‑1 (MQ=255)
                                                                                                           cACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcg                                     <  2:1665659/148‑1 (MQ=255)
                                                                                                           cACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcg                                     >  2:2084630/1‑148 (MQ=255)
                                                                                                           cACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGCTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcg                                     <  2:2934883/148‑1 (MQ=255)
                                                                                                             ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTcgcg                                   >  1:2349241/1‑148 (MQ=255)
                                                                                                              cTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt                                  >  2:3000675/1‑148 (MQ=255)
                                                                                                                   ccTCTTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                             >  2:692948/1‑148 (MQ=255)
                                                                                                                    ctctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGtttt                            >  2:2667284/1‑148 (MQ=255)
                                                                                                                      ctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTaa                          <  1:1036792/148‑1 (MQ=255)
                                                                                                                      ctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTaa                          >  2:2034047/1‑148 (MQ=255)
                                                                                                                      ctTCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTaa                          <  1:3729242/148‑1 (MQ=255)
                                                                                                                       ttCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGt                                                   >  2:2510433/1‑122 (MQ=255)
                                                                                                                       ttCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGt                                                   <  1:2510765/122‑1 (MQ=255)
                                                                                                                       ttCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTAAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAg                         <  1:3303662/148‑1 (MQ=255)
                                                                                                                        tCCCACACTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGc                        >  1:1618837/1‑148 (MQ=255)
                                                                                                                           cacaCTGCATCAAGCGCCTGGGTGGTTTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGAATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAc                     <  1:1707269/148‑1 (MQ=255)
                                                                                                                            acacTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAcc                    >  2:2169051/1‑148 (MQ=255)
                                                                                                                            acacTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAcc                    >  2:240388/1‑148 (MQ=255)
                                                                                                                              acggCATCAAGCGCCGGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAACGGGAGCTTCCCGCCAGTTGCAGCAGAGAATCAACCAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGc                  <  1:3471345/145‑1 (MQ=255)
                                                                                                                              acTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGc                  <  1:1574226/148‑1 (MQ=255)
                                                                                                                               cTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGcc                 >  2:2274653/1‑148 (MQ=255)
                                                                                                                               cTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGcc                 >  1:1395405/1‑148 (MQ=255)
                                                                                                                               cTGCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGcc                 >  1:1598650/1‑148 (MQ=255)
                                                                                                                                 gCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCaa               >  2:589668/1‑148 (MQ=255)
                                                                                                                                 gCATCAAGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCaa               >  2:1957428/1‑148 (MQ=255)
                                                                                                                                       aGCGCCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAggg         <  2:1782378/148‑1 (MQ=255)
                                                                                                                                        gcgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGc        >  1:865815/1‑148 (MQ=255)
                                                                                                                                        gcgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGc        >  2:839279/1‑148 (MQ=255)
                                                                                                                                         cgcCTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCg       >  2:3257106/1‑148 (MQ=255)
                                                                                                                                           ccTGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAg     <  1:2174987/148‑1 (MQ=255)
                                                                                                                                             tGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa   <  2:2688111/148‑1 (MQ=255)
                                                                                                                                             tGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa   <  2:1906793/148‑1 (MQ=255)
                                                                                                                                             tGGCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa   <  1:1224591/148‑1 (MQ=255)
                                                                                                                                             gggCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCTGTTGCAGCAGAGCACCAAACACCGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa   <  1:2576921/147‑1 (MQ=255)
                                                                                                                                              ggCTGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt  >  2:1080401/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAT  >  CP009273/1530884‑1531173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑