Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,557,309 C→A 16.4% D9Y (GAT→TAT)  ddpX ← D‑ala‑D‑ala dipeptidase, Zn‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,557,3090CA16.4% 816.2 / 91.8 273D9Y (GAT→TAT) ddpXD‑ala‑D‑ala dipeptidase, Zn‑dependent
Reads supporting (aligned to +/- strand):  ref base C (115/113);  new base A (22/23);  total (137/136)
Fisher's exact test for biased strand distribution p-value = 8.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GGCGATGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTG  >  CP009273/1557163‑1557456
                                                                                                                                                  |                                                                                                                                                   
 gCGATGCTGATACTTGTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCaa                                                                                                                                                   <  2:2555570/148‑1 (MQ=255)
  cGATGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAAc                                                                                                                                                  <  1:3575390/148‑1 (MQ=255)
    aTGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCa                                                                                                                                                >  2:211134/1‑148 (MQ=255)
    aTGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCa                                                                                                                                                >  2:517335/1‑148 (MQ=255)
     tGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAg                                                                                                                                               >  1:1920426/1‑148 (MQ=255)
     tGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAg                                                                                                                                               <  1:264377/148‑1 (MQ=255)
      gCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGt                                                                                                                                              >  1:2564183/1‑148 (MQ=255)
          aTACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCgg                                                                                                                                          <  1:299540/148‑1 (MQ=255)
           tACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCggt                                                                                                                                         >  2:2282199/1‑148 (MQ=255)
             cTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCggtgg                                                                                                                                       >  1:149639/1‑148 (MQ=255)
              ttttCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCggtggt                                                                                                                                      <  1:2594234/148‑1 (MQ=255)
                  cGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATcc                                                                                                                                  >  2:3089507/1‑148 (MQ=255)
                    ccAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGa                                                                                                                                <  2:123285/148‑1 (MQ=255)
                                    aTCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGt                                                                                                                <  2:404521/148‑1 (MQ=255)
                                       cTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGtata                                                                                                             >  1:2730310/1‑148 (MQ=255)
                                             tAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACaa                                                                                                       <  2:3204854/148‑1 (MQ=255)
                                             tAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACaa                                                                                                       >  2:2057178/1‑148 (MQ=255)
                                                cAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACaaaaa                                                                                                    <  1:2680427/148‑1 (MQ=255)
                                                   aCAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTGAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtg                                                                                                 >  1:1952815/1‑148 (MQ=255)
                                                   aCAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtg                                                                                                 >  1:2434913/1‑148 (MQ=255)
                                                     aaCGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCCGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgct                                                                                               <  1:1583759/148‑1 (MQ=255)
                                                      aCGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgctg                                                                                              >  2:2233457/1‑148 (MQ=255)
                                                      aCGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATtgctg                                                                                              <  2:695006/148‑1 (MQ=255)
                                                          gcTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCt                                                                                          <  2:2030215/148‑1 (MQ=255)
                                                           cTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCtt                                                                                         >  1:2084760/1‑148 (MQ=255)
                                                            ttGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCttt                                                                                        <  1:1499362/148‑1 (MQ=255)
                                                               cTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCg                                                                                     >  2:2755627/1‑148 (MQ=255)
                                                               cTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCg                                                                                     >  2:840994/1‑148 (MQ=255)
                                                                tGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGc                                                                                    >  1:3080920/1‑148 (MQ=255)
                                                                 gATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCCCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCt                                                                                   >  1:3199554/1‑148 (MQ=255)
                                                                  aTAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCtt                                                                                  <  2:1786276/148‑1 (MQ=255)
                                                                  aTAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCtt                                                                                  <  2:1465163/148‑1 (MQ=255)
                                                                  aTAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCtt                                                                                  >  2:2978023/1‑148 (MQ=255)
                                                                  aTAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCtt                                                                                  >  2:1006661/1‑148 (MQ=255)
                                                                      aTAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGc                                                                              >  1:1065913/1‑148 (MQ=255)
                                                                        aGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAg                                                                            >  2:1394118/1‑148 (MQ=255)
                                                                        aGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAg                                                                            >  2:3297743/1‑148 (MQ=255)
                                                                         gCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGa                                                                           >  2:2998336/1‑148 (MQ=255)
                                                                            ttACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGAtct                                                                        >  1:2901795/1‑148 (MQ=255)
                                                                            ttACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGAtct                                                                        <  1:3490523/148‑1 (MQ=255)
                                                                             tACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGAtctc                                                                       <  2:3577207/148‑1 (MQ=255)
                                                                               ccTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcat                                                                     >  2:3159905/1‑148 (MQ=255)
                                                                                cTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatg                                                                    <  1:1097792/148‑1 (MQ=255)
                                                                                 tgtgATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgc                                                                   >  2:1111554/1‑148 (MQ=255)
                                                                                  gtgATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTcatgcc                                                                  <  2:187688/148‑1 (MQ=255)
                                                                                       gTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCatgccatgcc                                                             >  2:2724099/1‑148 (MQ=255)
                                                                                           tCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGa                                                         >  1:2164740/1‑148 (MQ=255)
                                                                                           tCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGa                                                         >  1:2411797/1‑148 (MQ=255)
                                                                                            cAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                        >  1:2496289/1‑148 (MQ=255)
                                                                                            cAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                        >  1:3247341/1‑148 (MQ=255)
                                                                                            cAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGAt                                                        >  1:2698587/1‑148 (MQ=255)
                                                                                              gcgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATaa                                                      >  1:613099/1‑148 (MQ=255)
                                                                                               cgcAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAg                                                     >  1:2504643/1‑148 (MQ=255)
                                                                                                  aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAgcgc                                                  <  2:1916936/148‑1 (MQ=255)
                                                                                                  aGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAgcgc                                                  <  2:2356224/148‑1 (MQ=255)
                                                                                                   ggCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGcc                                                 >  2:2734117/1‑148 (MQ=255)
                                                                                                   ggCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGcc                                                 >  2:2734026/1‑148 (MQ=255)
                                                                                                       tATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGaa                                             <  1:3658524/148‑1 (MQ=255)
                                                                                                         tttCAATTCGATCTCCAGATCAGGGGAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                           >  2:2121437/1‑148 (MQ=255)
                                                                                                         tttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                           <  2:3514475/148‑1 (MQ=255)
                                                                                                         tttCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATg                                           <  2:502775/148‑1 (MQ=255)
                                                                                                             aagtCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGATTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTgg                                       <  2:1908534/145‑1 (MQ=255)
                                                                                                             aaTTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTgg                                       <  2:1086221/148‑1 (MQ=255)
                                                                                                              aTTCGATCTCCCGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGc                                      <  1:168284/148‑1 (MQ=255)
                                                                                                              aTTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGc                                      <  1:1013061/148‑1 (MQ=255)
                                                                                                              aTTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGc                                      >  1:1272995/1‑148 (MQ=255)
                                                                                                               ttCGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCt                                     >  2:250012/1‑148 (MQ=255)
                                                                                                                 cGATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTa                                   <  1:779587/148‑1 (MQ=255)
                                                                                                                  gATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaa                                  <  1:228535/148‑1 (MQ=255)
                                                                                                                  gATCTCCAGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTaa                                  <  1:1363103/148‑1 (MQ=255)
                                                                                                                     ctcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGt                               >  2:2631182/1‑148 (MQ=255)
                                                                                                                     ctcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGt                               >  1:3631178/1‑148 (MQ=255)
                                                                                                                      tcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGtt                              <  2:2084760/148‑1 (MQ=255)
                                                                                                                      tcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGtt                              <  2:2084806/148‑1 (MQ=255)
                                                                                                                      tcCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGtt                              <  1:3749068/148‑1 (MQ=255)
                                                                                                                       ccAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTta                             <  1:16635/148‑1 (MQ=255)
                                                                                                                       ccAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTta                             <  1:646999/148‑1 (MQ=255)
                                                                                                                        cAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtat                            <  1:534027/148‑1 (MQ=255)
                                                                                                                         aGATCAGGGAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTtata                           <  1:3041772/148‑1 (MQ=255)
                                                                                                                          gATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTatat                          <  2:647179/148‑1 (MQ=255)
                                                                                                                           aTCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtt                         <  1:2817315/148‑1 (MQ=255)
                                                                                                                            tCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAttt                        <  2:3652063/148‑1 (MQ=255)
                                                                                                                             cAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttt                       >  1:1756459/1‑148 (MQ=255)
                                                                                                                               gggAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttttt                     <  1:433502/148‑1 (MQ=255)
                                                                                                                               gggAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATAtttttt                     >  1:2223176/1‑148 (MQ=255)
                                                                                                                                ggAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACAGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTa                    <  1:3117962/148‑1 (MQ=255)
                                                                                                                                 gAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTaa                   <  1:2953247/148‑1 (MQ=255)
                                                                                                                                 gAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTaa                   >  1:966340/1‑148 (MQ=255)
                                                                                                                                 gAAGATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTaa                   <  1:2429111/148‑1 (MQ=255)
                                                                                                                                   aGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACt                 <  2:2434913/148‑1 (MQ=255)
                                                                                                                                   aGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACt                 <  1:18327/148‑1 (MQ=255)
                                                                                                                                    gATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACtt                <  2:2386948/148‑1 (MQ=255)
                                                                                                                                    gATTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACtt                >  1:2755229/1‑148 (MQ=255)
                                                                                                                                     aTTACGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACttt               <  2:149639/148‑1 (MQ=255)
                                                                                                                                      ttACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACtttt              >  1:1302681/1‑148 (MQ=255)
                                                                                                                                        aCGGCTAAATAAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGa            <  2:1272102/148‑1 (MQ=255)
                                                                                                                                                 tCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACAttt   >  2:176561/1‑148 (MQ=255)
                                                                                                                                                  cAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtg  <  1:3466922/148‑1 (MQ=255)
                                                                                                                                                  cAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtg  >  2:1345427/1‑148 (MQ=255)
                                                                                                                                                  cAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTtg  <  1:1186039/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGCGATGCTGATACTTTTCGCCAGCGCGGTAATCGCATCCTTGTGTAACAGACAACGCGCTTGCTGATAAATAGCTTTACCTGTGATGTTATCAGCGCAGGCGTATTTCAATTCGATCTCCAGATCAGGGAAGATTACGGCTAAATCAACCAGTTCGGTGGTATCCGACATAAACGGTTCCTGTATAAGACAAAAATTGCTGCGCTTTCCGCTTATGCAGATCTCATGCCATGCCGGGATAAGCGCCAGAATGCTGGCTTAAAGTTATATTTTTTAACTTTTGATCAACATTTG  >  CP009273/1557163‑1557456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑