Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 135,689 A→T 48.0% L675Q (CTG→CAG)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273135,6890AT48.0% 21.1 / inf 227L675Q (CTG→CAG) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (42/76);  new base T (61/48);  total (103/124)
Fisher's exact test for biased strand distribution p-value = 2.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

Only 100 of 229 total aligned reads displayed.

AACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCT  >  CP009273/135542‑135833
                                                                                                                                                   |                                                                                                                                                
aaCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCa                                                                                                                                                  <  1:1254889/148‑1 (MQ=255)
 aCAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAg                                                                                                                                                 >  1:352177/1‑148 (MQ=255)
    gCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTgcgc                                                                                                                                              >  2:1126707/1‑148 (MQ=255)
     cACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGcc                                                                                                                                             >  1:1122419/1‑148 (MQ=255)
      aCGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCg                                                                                                                                            <  2:268259/148‑1 (MQ=255)
         ttACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCgaga                                                                                                                                         <  1:2836943/148‑1 (MQ=255)
         ttACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCgaga                                                                                                                                         <  2:1950653/148‑1 (MQ=255)
          tACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGat                                                                                                                                        <  2:2572683/148‑1 (MQ=255)
          tACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGat                                                                                                                                        <  1:567261/148‑1 (MQ=255)
            cccGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGatat                                                                                                                                      >  1:509426/1‑148 (MQ=255)
            cccGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGatat                                                                                                                                      <  1:424103/148‑1 (MQ=255)
                 cgTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAAccc                                                                                                                                 <  2:1718505/148‑1 (MQ=255)
                   tGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCa                                                                                                                               <  1:1898105/148‑1 (MQ=255)
                   tGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCa                                                                                                                               <  1:2790656/148‑1 (MQ=255)
                     gagaTTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATg                                                                                                                             >  2:2790695/1‑148 (MQ=255)
                        aTTGGTCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctg                                                                                                                          >  2:2019212/1‑148 (MQ=255)
                         ttGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctgg                                                                                                                         <  1:77542/148‑1 (MQ=255)
                            gcccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctggctg                                                                                                                      >  2:1062581/1‑148 (MQ=255)
                            gcccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctggctg                                                                                                                      >  2:3278833/1‑148 (MQ=255)
                            gcccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATgctggctg                                                                                                                      >  2:334144/1‑148 (MQ=255)
                            gcccgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATgctggctg                                                                                                                      <  2:2577413/148‑1 (MQ=255)
                               cgcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGttt                                                                                                                   <  1:1195080/148‑1 (MQ=255)
                                gcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTa                                                                                                                  <  1:1705005/148‑1 (MQ=255)
                                gcccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTa                                                                                                                  <  1:320439/148‑1 (MQ=255)
                                  ccAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACa                                                                                                                <  2:213799/148‑1 (MQ=255)
                                    aGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATg                                                                                                              >  1:1689785/1‑148 (MQ=255)
                                        tCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAg                                                                                                          <  2:3521191/148‑1 (MQ=255)
                                        tCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAg                                                                                                          <  1:3584541/148‑1 (MQ=255)
                                           gCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGAcc                                                                                                       >  2:1451047/1‑148 (MQ=255)
                                              tACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCaaa                                                                                                    >  1:886448/1‑148 (MQ=255)
                                              tACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCaaa                                                                                                    <  2:1480916/148‑1 (MQ=255)
                                                   atatTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTg                                                                                               <  1:2045089/148‑1 (MQ=255)
                                                    tatTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTga                                                                                              <  2:229379/148‑1 (MQ=255)
                                                     atTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTgag                                                                                             <  1:3490128/148‑1 (MQ=255)
                                                     atTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTgag                                                                                             >  1:536399/1‑148 (MQ=255)
                                                          aCGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGac                                                                                        >  1:2278062/1‑147 (MQ=255)
                                                             gCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACgg                                                                                     >  2:1405143/1‑148 (MQ=255)
                                                              cACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACggg                                                                                    <  1:692847/148‑1 (MQ=255)
                                                               aCCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGt                                                                                   <  1:3750324/148‑1 (MQ=255)
                                                               aCCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGt                                                                                   <  2:1628047/148‑1 (MQ=255)
                                                               aCCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGt                                                                                   <  2:226088/148‑1 (MQ=255)
                                                                  ggAACCGGCATCGGGAACGGTATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGa                                                                                <  1:2091706/148‑1 (MQ=255)
                                                                       cGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTg                                                                           <  1:2269156/148‑1 (MQ=255)
                                                                             cGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCa                                                                     >  2:3653402/1‑148 (MQ=255)
                                                                                gAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATAc                                                                  >  1:1825520/1‑148 (MQ=255)
                                                                                gAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATAc                                                                  >  1:901038/1‑148 (MQ=255)
                                                                                 aaCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACg                                                                 >  2:396674/1‑148 (MQ=255)
                                                                                 aaCGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACg                                                                 <  2:3197511/148‑1 (MQ=255)
                                                                                   cGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGt                                                               >  1:1510240/1‑148 (MQ=255)
                                                                                   cGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGt                                                               <  2:2551234/148‑1 (MQ=255)
                                                                                     gCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTac                                                             <  1:3338214/148‑1 (MQ=255)
                                                                                      cATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTaca                                                            <  1:3294494/148‑1 (MQ=255)
                                                                                         aCTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCg                                                         <  1:2605351/148‑1 (MQ=255)
                                                                                         aCTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCg                                                         >  1:2475889/1‑148 (MQ=255)
                                                                                         aCTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCg                                                         >  2:1224361/1‑148 (MQ=255)
                                                                                           tGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTa                                                       >  2:320203/1‑148 (MQ=255)
                                                                                           tGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTa                                                       <  2:904694/148‑1 (MQ=255)
                                                                                           tGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTa                                                       <  2:904796/148‑1 (MQ=255)
                                                                                              ccTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACtg                                                    >  1:947672/1‑148 (MQ=255)
                                                                                                     cgTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGa                                             <  1:1189972/148‑1 (MQ=255)
                                                                                                     cgTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGa                                             <  1:387383/148‑1 (MQ=255)
                                                                                                      gTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGaa                                            <  1:1832010/148‑1 (MQ=255)
                                                                                                        aCCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATg                                          <  2:1698132/148‑1 (MQ=255)
                                                                                                        aCCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATg                                          <  2:10769/148‑1 (MQ=255)
                                                                                                        aCCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATg                                          <  2:3611906/148‑1 (MQ=255)
                                                                                                         ccAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGc                                         >  1:3153750/1‑148 (MQ=255)
                                                                                                            aTACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCgg                                      <  2:2396800/148‑1 (MQ=255)
                                                                                                              aCGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGAt                                    >  2:2566816/1‑148 (MQ=255)
                                                                                                              aCGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGAt                                    <  1:334144/148‑1 (MQ=255)
                                                                                                                gTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTc                                  <  2:1003955/148‑1 (MQ=255)
                                                                                                                gTTTCTTCCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTc                                  <  2:3488970/148‑1 (MQ=255)
                                                                                                                 tttCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTcc                                 >  2:548805/1‑148 (MQ=255)
                                                                                                                  ttcttcCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  1:1667431/1‑148 (MQ=255)
                                                                                                                  ttcttcCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  2:2930118/1‑148 (MQ=255)
                                                                                                                  ttcttcCACACCACTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  2:3261699/1‑148 (MQ=255)
                                                                                                                  ttcttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                <  2:2165727/148‑1 (MQ=255)
                                                                                                                  ttcttcCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCg                                >  1:744993/1‑148 (MQ=255)
                                                                                                                          caccacTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGACCGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTg                        >  2:305920/1‑148 (MQ=255)
                                                                                                                          caccacTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTg                        >  2:1723302/1‑148 (MQ=255)
                                                                                                                            ccacTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGcc                      <  1:559546/148‑1 (MQ=255)
                                                                                                                            ccacTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGcc                      <  1:101293/148‑1 (MQ=255)
                                                                                                                               cTTCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCttt                   >  1:2162601/1‑148 (MQ=255)
                                                                                                                               cTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCttt                   >  2:3581099/1‑148 (MQ=255)
                                                                                                                                ttCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTg                  >  2:1511734/1‑148 (MQ=255)
                                                                                                                                ttCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTg                  >  1:1313859/1‑148 (MQ=255)
                                                                                                                                 tCATTAGTTTTCAGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTgg                 >  2:3108581/1‑148 (MQ=255)
                                                                                                                                    ttAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCAt              >  1:2096511/1‑148 (MQ=255)
                                                                                                                                    ttAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCAt              >  2:2416600/1‑148 (MQ=255)
                                                                                                                                     tAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATc             >  2:1340550/1‑148 (MQ=255)
                                                                                                                                       gTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCtt           <  1:2381523/148‑1 (MQ=255)
                                                                                                                                        ttttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCttt          >  1:1408443/1‑148 (MQ=255)
                                                                                                                                        ttttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCttt          <  2:2428479/148‑1 (MQ=255)
                                                                                                                                          ttCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCg        >  2:486815/1‑148 (MQ=255)
                                                                                                                                             aGATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgct     >  1:2833129/1‑148 (MQ=255)
                                                                                                                                              gATCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctg    >  1:2661761/1‑148 (MQ=255)
                                                                                                                                              gATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctg    <  2:3602160/148‑1 (MQ=255)
                                                                                                                                              gATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctg    <  2:791331/148‑1 (MQ=255)
                                                                                                                                               aTCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctgc   >  1:751823/1‑148 (MQ=255)
                                                                                                                                                tCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctgct  >  2:3240468/1‑148 (MQ=255)
                                                                                                                                                tCCTGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGgctgct  <  2:509426/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AACAGCACGTTACCCGCCGTGGAGATTGGCCCGCCCAGCATCGGCATACCCATATTGAACGGCACCGGAACCGGCATCGGGAACGGCATACTGTCCTGCGGCGTACCAATACGTTTCTTCCACACCACTTCATTAGTTTTCAGATCCAGCGCCGAGATATAACCCCATGCTGGCTGTTTACATGGCAGACCAAATGGTGAGAGGAACGGGTTGAGCGTGACACCATACGGTACACCGTACTGTGGCTGAATGCCGGATTCCGTACCCGTGCCTTTGGCATCTTTCGGCTGCT  >  CP009273/135542‑135833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑