Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 142,460 T→A 100% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273142,4600TA100.0% 719.6 / NA 218F298Y (TTC→TAC) yadEputative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (120/98);  total (120/98)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

ATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTC  >  CP009273/142316‑142604
                                                                                                                                                |                                                                                                                                                
 tCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGACATTCGCGATGTATTTGATTACCAg                                                                                                                                              >  2:1686853/1‑148 (MQ=255)
 tCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAg                                                                                                                                              >  2:1704690/1‑148 (MQ=255)
  ccTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGt                                                                                                                                             >  2:422097/1‑148 (MQ=255)
  ccTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAAAGAAATTCGCGATGTATTTGATTACCAGt                                                                                                                                             >  1:46093/1‑148 (MQ=255)
      tgtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTcaca                                                                                                                                         <  1:3560046/148‑1 (MQ=255)
       gtTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATTAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGCCACAt                                                                                                                                        <  1:1825701/148‑1 (MQ=255)
         tGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATAc                                                                                                                                      <  2:2492863/148‑1 (MQ=255)
             aCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAt                                                                                                                                  >  1:3815183/1‑148 (MQ=255)
             aCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAt                                                                                                                                  >  1:3814824/1‑148 (MQ=255)
             aCAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAt                                                                                                                                  >  1:3650462/1‑148 (MQ=255)
              cAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAtt                                                                                                                                 <  1:1019665/148‑1 (MQ=255)
                 tatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAttttt                                                                                                                              <  2:58701/148‑1 (MQ=255)
                 tatGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCAttttt                                                                                                                              >  1:3223477/1‑148 (MQ=255)
                      cATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCAt                                                                                                                         <  2:1317369/148‑1 (MQ=255)
                        tGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCg                                                                                                                       <  2:2624266/148‑1 (MQ=255)
                         gAAGGCGACGGCGTTTATTGTTAACTCACGCATCAAACGTCACCCCCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGATCTTAACGAAATTCGCGATTTATTTGATTACCAGTCACATAACCATTTTTTGCATAgg                                                                                                                      >  1:3522940/1‑148 (MQ=255)
                             gCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAg                                                                                                                  <  1:2589918/148‑1 (MQ=255)
                              cGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGa                                                                                                                 <  2:26602/148‑1 (MQ=255)
                                aCGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATg                                                                                                               <  1:5562/148‑1 (MQ=255)
                                  ggCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGt                                                                                                             <  1:1620219/148‑1 (MQ=255)
                                  ggCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGt                                                                                                             >  2:3783193/1‑148 (MQ=255)
                                       ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCTGCGGGTAGATGGTGATCg                                                                                                        >  1:1114403/1‑148 (MQ=255)
                                       ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                        >  2:372467/1‑148 (MQ=255)
                                       ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                        >  1:1114646/1‑148 (MQ=255)
                                       ttATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCg                                                                                                        >  2:2295511/1‑148 (MQ=255)
                                           tGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGa                                                                                                    >  1:2638206/1‑148 (MQ=255)
                                               aCCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCa                                                                                                <  1:2231766/148‑1 (MQ=255)
                                                    aCGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTa                                                                                           <  1:1982082/148‑1 (MQ=255)
                                                       cATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                        >  1:1267526/1‑148 (MQ=255)
                                                       cATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                        <  1:1802170/148‑1 (MQ=255)
                                                       cATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTg                                                                                        <  1:1799703/148‑1 (MQ=255)
                                                        aTCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGa                                                                                       <  2:660160/148‑1 (MQ=255)
                                                          cAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc                                                                                     <  1:3133324/148‑1 (MQ=255)
                                                          cAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGc                                                                                     >  2:1246608/1‑148 (MQ=255)
                                                            aaCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCg                                                                                   >  1:329085/1‑148 (MQ=255)
                                                            aaCGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCg                                                                                   >  2:1089965/1‑148 (MQ=255)
                                                                tCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAACTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCCTCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGt                                                                               >  2:2179814/1‑148 (MQ=255)
                                                                 cACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTg                                                                              <  2:1825783/148‑1 (MQ=255)
                                                                 cACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTg                                                                              <  1:28746/148‑1 (MQ=255)
                                                                  aCCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGa                                                                             >  1:2453364/1‑148 (MQ=255)
                                                                  aCCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGa                                                                             >  1:39407/1‑148 (MQ=255)
                                                                         gAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAat                                                                      <  1:2983985/148‑1 (MQ=255)
                                                                          aaGTGGAACACAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAata                                                                     >  2:1282313/1‑148 (MQ=255)
                                                                           aGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAatat                                                                    <  2:1552122/148‑1 (MQ=255)
                                                                            gTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATAtt                                                                   <  1:3042293/148‑1 (MQ=255)
                                                                            gTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATAtt                                                                   <  2:370053/148‑1 (MQ=255)
                                                                              ggAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCt                                                                 <  2:773069/148‑1 (MQ=255)
                                                                                      aaaTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtt                                                         >  1:1618257/1‑148 (MQ=255)
                                                                                        aTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtttt                                                       >  2:2434395/1‑148 (MQ=255)
                                                                                        aTCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGAtttt                                                       >  2:2916896/1‑148 (MQ=255)
                                                                                         tCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTg                                                      <  2:1661728/148‑1 (MQ=255)
                                                                                          cGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGc                                                     <  2:329085/148‑1 (MQ=255)
                                                                                          cGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGc                                                     >  2:1601889/1‑148 (MQ=255)
                                                                                           gCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCa                                                    <  2:91425/148‑1 (MQ=255)
                                                                                                aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTc                                               >  1:2934508/1‑148 (MQ=255)
                                                                                                aaTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTc                                               <  1:1199073/148‑1 (MQ=255)
                                                                                                 aTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                              >  2:2431246/1‑148 (MQ=255)
                                                                                                 aTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                              >  2:1780729/1‑148 (MQ=255)
                                                                                                 aTTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCa                                              >  2:1022222/1‑148 (MQ=255)
                                                                                                  tttATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCAc                                             >  1:1089507/1‑148 (MQ=255)
                                                                                                        aGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCcgcg                                       <  1:1588331/148‑1 (MQ=255)
                                                                                                         gCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCgcgc                                      >  1:654740/1‑148 (MQ=255)
                                                                                                         gCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCgcgc                                      >  1:817499/1‑148 (MQ=255)
                                                                                                           gTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGctc                                    <  1:1360400/148‑1 (MQ=255)
                                                                                                                 gAGCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGgcgc                              >  2:1487023/1‑148 (MQ=255)
                                                                                                                   gCTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCaa                            <  2:853253/148‑1 (MQ=255)
                                                                                                                    cTTAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCTGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAt                           <  2:1921566/148‑1 (MQ=255)
                                                                                                                      tAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt                         >  1:2681055/1‑148 (MQ=255)
                                                                                                                      tAACGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAAttt                         >  1:1640237/1‑148 (MQ=255)
                                                                                                                       aaCGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTa                        <  2:1123311/148‑1 (MQ=255)
                                                                                                                       aaCGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTa                        <  1:3160397/148‑1 (MQ=255)
                                                                                                                         cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                      <  2:2001916/148‑1 (MQ=255)
                                                                                                                         cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                      <  1:1704690/148‑1 (MQ=255)
                                                                                                                         cGAAATTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAAt                      <  2:499423/148‑1 (MQ=255)
                                                                                                                            aaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCg                   >  1:3037257/1‑148 (MQ=255)
                                                                                                                            aaTTCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCg                   >  1:1496990/1‑148 (MQ=255)
                                                                                                                              ttCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCa                 >  1:71391/1‑148 (MQ=255)
                                                                                                                              ttCGCGATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCa                 <  2:3208088/148‑1 (MQ=255)
                                                                                                                                cgcgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATg               <  2:3667681/148‑1 (MQ=255)
                                                                                                                                 gcgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGt              <  2:298229/148‑1 (MQ=255)
                                                                                                                                 gcgATGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGt              <  1:3433177/148‑1 (MQ=255)
                                                                                                                                    aTGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg           >  2:1144401/1‑148 (MQ=255)
                                                                                                                                    aTGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTg           <  1:2464287/148‑1 (MQ=255)
                                                                                                                                     tGTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTCTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTgg          >  2:235854/1‑148 (MQ=255)
                                                                                                                                      gTATTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGt         <  1:3341890/148‑1 (MQ=255)
                                                                                                                                        aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       <  2:2822904/148‑1 (MQ=255)
                                                                                                                                        aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       >  2:1361562/1‑148 (MQ=255)
                                                                                                                                        aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       <  2:3814859/148‑1 (MQ=255)
                                                                                                                                        aTTTGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       <  2:3815201/148‑1 (MQ=255)
                                                                                                                                        aTTTGATGACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTAt       >  2:3432423/1‑148 (MQ=255)
                                                                                                                                         tttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc      <  2:356107/148‑1 (MQ=255)
                                                                                                                                         tttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATc      <  1:998191/148‑1 (MQ=255)
                                                                                                                                          ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     >  1:3206069/1‑148 (MQ=255)
                                                                                                                                          ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     <  2:2771415/148‑1 (MQ=255)
                                                                                                                                          ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     >  1:3595840/1‑148 (MQ=255)
                                                                                                                                          ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     >  1:3729414/1‑148 (MQ=255)
                                                                                                                                          ttGATTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCt     >  1:791493/1‑148 (MQ=255)
                                                                                                                                            gATTACCAGTAACATACCCTTTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCttt   <  2:1467571/148‑1 (MQ=255)
                                                                                                                                             aTTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTc  >  2:3751761/1‑148 (MQ=255)
                                                                                                                                             aTTACCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTc  >  1:46669/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ATCCTGTGTTGAAACAATATGGCATGAAGGCGACGGCGTTTATTGTTACCTCACGCATCAAACGTCACCCGCAGAAGTGGAACCCAAAATCGCTGCAATTTATGAGCGTTTCTGAGCTTAACGAAATTCGCGATGTATTTGATTTCCAGTCACATACCCATTTTTTGCATCGGGTAGATGGTTATCGCCGACCCATATTACTGAGCCGTAGTGAGCACAATATTCTGTTTGATTTTGCACGTTCACGCCGCGCTCTGGCGCAATTTAATCCGCATGTCTGGTATCTTTC  >  CP009273/142316‑142604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑