Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,673,380 T→A 7.4% S233T (TCA→ACA)  ydgH → DUF1471 family periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,673,3800TA 7.4% 393.7 / 4.6 152S233T (TCA→ACA) ydgHDUF1471 family periplasmic protein
Reads supporting (aligned to +/- strand):  ref base T (64/74);  new base A (2/11);  total (67/85)
Fisher's exact test for biased strand distribution p-value = 4.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

AGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGG  >  CP009273/1673233‑1673524
                                                                                                                                                   |                                                                                                                                                
agCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGtcat                                                                                                                                                  <  2:3024118/148‑1 (MQ=255)
agCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGtcat                                                                                                                                                  <  1:3081799/148‑1 (MQ=255)
agCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGtcat                                                                                                                                                  >  2:3659859/1‑148 (MQ=255)
agCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGtcat                                                                                                                                                  <  2:3676205/148‑1 (MQ=255)
     ggATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCaaaa                                                                                                                                             <  2:3058666/148‑1 (MQ=255)
      gATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACCAGACATCaaaac                                                                                                                                            >  1:2583779/1‑147 (MQ=255)
       aTGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAgg                                                                                                                                           <  2:246491/148‑1 (MQ=255)
        tgtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCCGTCATCAAAAggc                                                                                                                                          >  1:2804857/1‑148 (MQ=255)
        tgtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggc                                                                                                                                          >  1:3388305/1‑148 (MQ=255)
         gtgATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCCGTCATCAAAAggcg                                                                                                                                         >  1:3164006/1‑148 (MQ=255)
            aTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgggc                                                                                                                                      >  1:3116495/1‑148 (MQ=255)
             tCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAggcgggcg                                                                                                                                     <  1:2275533/148‑1 (MQ=255)
                cGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTa                                                                                                                                  <  1:1125000/148‑1 (MQ=255)
                cGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTa                                                                                                                                  >  2:917799/1‑148 (MQ=255)
                      aTTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGt                                                                                                                            <  2:2834528/148‑1 (MQ=255)
                      aTTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGt                                                                                                                            >  2:598448/1‑148 (MQ=255)
                       ttCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCCAAAGGCGGGCGTTACACCGTc                                                                                                                           >  2:3477568/1‑148 (MQ=255)
                       ttCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTc                                                                                                                           <  1:21086/148‑1 (MQ=255)
                       ttCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTc                                                                                                                           <  1:2481470/148‑1 (MQ=255)
                        tCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGACGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCa                                                                                                                          >  2:2163431/1‑148 (MQ=255)
                          cGAAGCAGTACGTGCAGCTCTGGCTTCCGGTTGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACg                                                                                                                        <  1:156878/148‑1 (MQ=255)
                          cGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACg                                                                                                                        <  1:2618380/148‑1 (MQ=255)
                                  gACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGa                                                                                                                >  1:2493212/1‑148 (MQ=255)
                                     gTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATgg                                                                                                             <  1:1520369/148‑1 (MQ=255)
                                           ctctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACtca                                                                                                       >  2:739591/1‑146 (MQ=255)
                                           ctctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTaa                                                                                                       <  2:262912/148‑1 (MQ=255)
                                           ctctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTaa                                                                                                       >  2:1869042/1‑148 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg                                                                                                     <  1:1476373/148‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAg                                                                                                     <  1:1433513/148‑1 (MQ=255)
                                             ctGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCGGCGTTGTAAACCCCTCATAAAAAAGCCGGCGGTTCACCCGCCCGCTCCCCGATGGCACAAAAg                                                                                                     >  1:1624789/1‑148 (MQ=255)
                                                 cTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCga                                                                                                 <  1:2864814/148‑1 (MQ=255)
                                                 cTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCga                                                                                                 >  2:3164515/1‑148 (MQ=255)
                                                     cGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCgaagaa                                                                                             <  1:1122925/148‑1 (MQ=255)
                                                      ggTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAATGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAAc                                                                                            >  1:866771/1‑148 (MQ=255)
                                                         ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGa                                                                                         <  1:404818/148‑1 (MQ=255)
                                                         ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGa                                                                                         <  1:404634/148‑1 (MQ=255)
                                                         ggCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGa                                                                                         <  1:404563/148‑1 (MQ=255)
                                                             aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaa                                                                                     >  2:1007576/1‑148 (MQ=255)
                                                             aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaa                                                                                     <  2:915696/148‑1 (MQ=255)
                                                             aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaa                                                                                     >  2:2795626/1‑148 (MQ=255)
                                                             aaGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaa                                                                                     <  2:915643/148‑1 (MQ=255)
                                                              aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa                                                                                    <  1:675202/148‑1 (MQ=255)
                                                              aGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACaaa                                                                                    >  1:2823001/1‑148 (MQ=255)
                                                               gCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAg                                                                                   >  2:1853441/1‑148 (MQ=255)
                                                                ccGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTATTTTAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACCCTCCCCGATGGCACAAAAGTCGAAGAACAGAACAAAGc                                                                                  >  1:2622330/1‑148 (MQ=255)
                                                                 cgcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAATCg                                                                                 >  1:1219697/1‑148 (MQ=255)
                                                                 cgcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCg                                                                                 <  2:3788342/148‑1 (MQ=255)
                                                                 cgcgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACAAGTAATCAAAAGGCGGGCGTTACAACGTCACGCTCCCGGATGGCACAAAAGTCGAAGAACTGAACAAAGCg                                                                                 >  1:1220666/1‑148 (MQ=255)
                                                                  gcgAAGAAAGTTGAGATCCGGGGTGTTGCGACTACCGCATCACAATGTTCTGAAGTGGGTCGTTTCTTTGAAACCCTGTAATCAAATGGGGGGCTTTAAACGTTCACGCTCCCGTTTGGCACTAATGTCTAATAACTTAACAAAGCGa                                                                                <  2:2566011/148‑1 (MQ=255)
                                                                   cgAAGATAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCTTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGAc                                                                               <  2:188641/148‑1 (MQ=255)
                                                                   cgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGAc                                                                               <  2:3362701/148‑1 (MQ=255)
                                                                   cgAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCGCCCGGATGGCACTAAAGTCGAAGAAATGAACAAAGCGAc                                                                               >  2:1637688/1‑148 (MQ=255)
                                                                    taataatgTTGAGATCCCGGGTGTTGCGACTACCGAATAACAAAGTTGTGAAGTCGGTCGCTTTTTTGAAACCCAGTCATCAAACGGGGGGCGTTACACCTACACGCTCCCGTATGGCAATAACGTCTAATAACTTAACAAAGCGAcc                                                                              <  2:2202932/141‑1 (MQ=255)
                                                                     aagaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCg                                                                             >  2:2359464/1‑148 (MQ=255)
                                                                       gaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCa                                                                           >  1:3497655/1‑148 (MQ=255)
                                                                       gaaAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCGTCTTTTAAAACCAAACCAAAAAACGCGGGCCTTAAACCCACAAACTCCCCGATGGCACAAAAATAGAACAACTTAAAAAAACCACCGCa                                                                           >  1:1668239/1‑148 (MQ=255)
                                                                           gTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCACTAAAGTCGAATAACTGAACAAAGCGACCGCAGCga                                                                       <  2:2516906/148‑1 (MQ=255)
                                                                                gaTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGatgatg                                                                  >  2:2007783/1‑148 (MQ=255)
                                                                                gaTCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATAAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGatgatg                                                                  >  2:1423799/1‑148 (MQ=255)
                                                                                 agccggggTGTTGCGACTACCGCATCACCATGTTCTGAAGTCGGTCGCTTCTTTGAACCCCAGTCATCAAACGGGGGGCGTTACCCCGTCCCGCTCCGGGTTGGCACTAACGTCGAAGAACTGAACAACGCGACCGCAGCGATGATgg                                                                 <  2:748767/143‑1 (MQ=255)
                                                                                  tCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTGTTTTAAAACCAGTCATCAAAAGGCGGGCGGTACACCGTCACCCTCCCGGATGGCCCAAAAGTCGAAGAACAGAACAAAGCGACCCCAGCGATGTTGGt                                                                >  1:1327549/1‑148 (MQ=255)
                                                                                  tCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTGTTTTAAAACCAGGCATCAAAATGCGGGCGTTACACCCTCACCCTCCCGGATGGGCCTAAAGTAGAAGAAATTAAAAAAGCGACCGCACCGATGTTGGt                                                                >  1:1327521/1‑148 (MQ=255)
                                                                                  tCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCGCCCGGCTGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCCGCGATGATGGt                                                                >  1:3669702/1‑148 (MQ=255)
                                                                                   ccggggTGTTGCGACTCCCGAATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGGGTTACACCGGCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTc                                                               <  2:2644604/145‑1 (MQ=255)
                                                                                   cccGGTTGTTGCGACTACCGCATCACCACGTTCTGACGTCGGTCGCTTCTTTGAAACCCAGTCAACAAACGGCGGGCGTTACACCGACACGCTCCCGGATGGCACTAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTc                                                               <  2:2644632/148‑1 (MQ=255)
                                                                                   cccGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTc                                                               >  2:1430017/1‑148 (MQ=255)
                                                                                       ggTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCGTTGAAACCCCCTCATCAAAAGGCGGGCCTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGCACAAAGCGACCGCAGCGAAGATGGTcccct                                                           >  2:1972042/1‑146 (MQ=255)
                                                                                       ggTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGATTATTGGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCGGGTTGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGt                                                           <  2:2392820/148‑1 (MQ=255)
                                                                                           ttGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGa                                                       >  2:864659/1‑148 (MQ=255)
                                                                                           ttGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTATTTGAAACCCAGTCAACAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCCAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGa                                                       <  2:2260431/148‑1 (MQ=255)
                                                                                                     cGCATCACCACGTTCTGAAGTCGGTCGCTTGTTTTAAACCCTGTCATCAAAAGGCGGGCGTTACACCGACACGCTCCCGGATGGCACTAAAGTCTAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa                                             <  2:3313603/148‑1 (MQ=255)
                                                                                                     cGCATCACCAAGTTCTTAAGTCGGTCGCTTCTTTCAAACCCAGACATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCACTAACGTCCAATAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa                                             <  2:3313776/148‑1 (MQ=255)
                                                                                                     cGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCaaa                                             >  2:2105878/1‑148 (MQ=255)
                                                                                                          cACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGGCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAACAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                        <  2:1976467/148‑1 (MQ=255)
                                                                                                          cACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAAACCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATtctc                                        >  2:2071808/1‑148 (MQ=255)
                                                                                                            ccATGTTCTGACGGCGGGCGCTTCTTTCAAACCCAGGCAAAAACCGGCGGGCGTAACACCGACACGCGCCCGTTTGCAACTAACGTCCAAGAACTCAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTg                                      <  1:1982134/148‑1 (MQ=255)
                                                                                                                 ttCTGAAGTCGGTCGCTTCTTTTAAAACCACTCATAAAAAGGCGGGCGTTAAACCCGCACGCTCCCGGATGGCACAAAAGTCGAAGAACAGAACAAATCCACCGCAGCGATGATGGTCCCGTTCGACAGCATAAAAATCTCATGCAAc                                 >  1:2341744/1‑148 (MQ=255)
                                                                                                                 ttCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAAc                                 >  2:2229158/1‑148 (MQ=255)
                                                                                                                 ttCTGAAGTCGGTCGCTTATTTTAAACCCAGTCATCAAAAGGCGGGCGTTACAACGTAACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTACCGTTCGACAGCATCAAATTCTCTGGCAAc                                 >  2:2229065/1‑148 (MQ=255)
                                                                                                                      aaGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACAGAACAAAGCGACCGCAGCGATGATGGTCCCGGTCGACAGCATCAAATTCTCTGGCAACTAAgg                            >  2:125148/1‑148 (MQ=255)
                                                                                                                      aaGTCGGTCGCTTCTTTGAAACCCAGTAAAAAAAAGGCGGGCGTTAAACCCTCACGCTCCCGGATGGCACTAAAGTCGAAGAACATAACAAAGCGACCGCAGCGAAGATGGTCCCGTTCGACAGCATCCAATTCTATGGCAAcaaagg                            >  2:125238/1‑143 (MQ=255)
                                                                                                                          gggtcgCTTCTTTCACACCCCCACAACAACCGGGGGGCGTCACCCCGACCCGCGCCGGTCTGCCAATAACGTCGAACAACCCAAAACCGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAAc                        <  1:1312660/147‑1 (MQ=255)
                                                                                                                              ctcttattTGAAACCCAGTCATCAAATGGCGGGGGTTAAACCGTCACGCTCCTGGATGGAACTAACGGCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                    <  2:2405980/146‑1 (MQ=255)
                                                                                                                              cgattcttTCAACCCCCGCAAACAAATGGGGGGCTTTACACCGTCACGCTCCCGGATGGCACTAACGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                    <  1:1562827/145‑1 (MQ=255)
                                                                                                                              cgCTTGTTTGAAACACATTCATCAAATGGCGGGCGTTACACCGTCAGGCGCCCTTATGGCACTAAAGTCGAACAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCATCTACGGCAACATGa                    <  2:1858212/148‑1 (MQ=255)
                                                                                                                              cgCTTCTTTTAAACCCAGTCAAAAAAAGGCGGGCGTTACACCGACCCGCTCCCGGATGGCACAAACCTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                    <  2:2768432/148‑1 (MQ=255)
                                                                                                                              cgCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGTATGGCACTAAAGTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGa                    <  2:2406066/148‑1 (MQ=255)
                                                                                                                               gCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGAc                   >  1:595097/1‑148 (MQ=255)
                                                                                                                                   atttGAAACCCATAAATCAAAAGGCGGGCGTAACACCGTCACGCTCCCGGATGGCACTAAAGTCCAAGAACTTAAAAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGaa               <  1:1595759/147‑1 (MQ=255)
                                                                                                                                     tttaaaCCCATTAATAAAACGGGGGGGTTAACACCGACACGCGCCGGGTTGCCAATAACGACCAACAACTCAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGt             <  1:1371589/145‑1 (MQ=255)
                                                                                                                                     ttGAAACCCAGTCATCAAACGGGGGGCGTTACACCTTCACGCGCCCGGATGGCACTAACGTCGAAGAACTTAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGt             <  1:962113/148‑1 (MQ=255)
                                                                                                                                     ttGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGt             >  1:2765471/1‑148 (MQ=255)
                                                                                                                                       gAAACCAAGTAAACAAATGGCGGGCTTTACACCGTCACGCTCCCGGTTGGCAATAAAGTCGAAGAACTTAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATGTTTTTCTCTGGCAACTACGGCAACATGACCGAAGtct           <  2:2493212/148‑1 (MQ=255)
                                                                                                                                        aaaCCCAGTCCTCAAAAGGGGGGCGTTACACCGTCACGCTCCCGGTTGGCAATAACGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc          <  2:3164006/148‑1 (MQ=255)
                                                                                                                                        aaaCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCACCATGACCGAAGtctc          >  2:3479824/1‑148 (MQ=255)
                                                                                                                                        aaaCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc          >  1:2826317/1‑148 (MQ=255)
                                                                                                                                        aaaCCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGtctc          >  1:3184942/1‑148 (MQ=255)
                                                                                                                                         aaCCCACTCAAAAAAAGGGGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCTACCAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTcc         <  2:96907/148‑1 (MQ=255)
                                                                                                                                            ccAGTCAACAAACGGCGGGCTTTACCCCGTCACGCTCCCGGATGCCACTAACGTCGAAGAACTGAACAACGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTAt      <  1:3724529/148‑1 (MQ=255)
                                                                                                                                               gTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAACAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAg   <  2:491257/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAGTCATCAAAAGGCGGGCGTTACACCGTCACGCTCCCGGATGGCACTAAAGTCGAAGAACTGAACAAAGCGACCGCAGCGATGATGGTCCCGTTCGACAGCATCAAATTCTCTGGCAACTACGGCAACATGACCGAAGTCTCCTATCAGG  >  CP009273/1673233‑1673524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑