Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,723,809 G→T 100% E156* (GAA→TAA)  lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,723,8090GT98.1% 665.3 / 3.3 207E156* (GAA→TAA) lhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base T (105/98);  total (108/99)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCT  >  CP009273/1723664‑1723951
                                                                                                                                                 |                                                                                                                                              
cccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTaa                                                                                                                                              >  2:1363887/1‑148 (MQ=255)
 ccGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAAt                                                                                                                                             <  2:333920/148‑1 (MQ=255)
  cGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATc                                                                                                                                            <  1:1925345/148‑1 (MQ=255)
    ttGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCAc                                                                                                                                          >  2:1715988/1‑148 (MQ=255)
         gggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                     <  2:2260714/148‑1 (MQ=255)
         gggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                     >  2:3481866/1‑148 (MQ=255)
         gggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTAt                                                                                                                                     <  1:1372150/148‑1 (MQ=255)
          ggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                    >  1:1880019/1‑148 (MQ=255)
          ggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                    >  1:1880340/1‑148 (MQ=255)
          ggTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATc                                                                                                                                    >  1:1087561/1‑148 (MQ=255)
             aTTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGa                                                                                                                                 >  2:3712126/1‑148 (MQ=255)
             aTTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGa                                                                                                                                 <  1:3286377/148‑1 (MQ=255)
               tGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATg                                                                                                                               >  1:842995/1‑148 (MQ=255)
                       aaCGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTAGTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTc                                                                                                                       <  2:1873768/148‑1 (MQ=255)
                         cggcggcggCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGCTATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTccc                                                                                                                     >  2:2542383/1‑148 (MQ=255)
                                     ggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGaaa                                                                                                         <  1:2731298/148‑1 (MQ=255)
                                     ggcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGaaa                                                                                                         <  2:1539127/148‑1 (MQ=255)
                                      gcgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAAc                                                                                                        >  2:677333/1‑148 (MQ=255)
                                       cgAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGAGGCTGACCTCCCGCGCGCGCGAAACg                                                                                                       <  2:2969935/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                     <  1:2587237/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                     >  1:3642350/1‑148 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                     <  1:2587247/148‑1 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                     >  1:1415989/1‑148 (MQ=255)
                                         aaaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACAACCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCt                                                                                                     >  1:1745780/1‑148 (MQ=255)
                                          aaCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTa                                                                                                    <  1:3579592/148‑1 (MQ=255)
                                           aCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAc                                                                                                   >  1:3729309/1‑148 (MQ=255)
                                           aCGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAc                                                                                                   >  2:2199332/1‑148 (MQ=255)
                                            cGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcg                                                                                                  >  2:2044507/1‑148 (MQ=255)
                                            cGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTAcg                                                                                                  >  2:1707911/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGGCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                               >  2:153526/1‑148 (MQ=255)
                                               aaGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcgg                                                                                               <  1:1693868/148‑1 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACg                                                                                                       >  1:1047422/1‑138 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcggcg                                                                                             <  2:2751302/148‑1 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcggcg                                                                                             >  2:588876/1‑148 (MQ=255)
                                                 gTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACgcggcg                                                                                             >  1:1270632/1‑148 (MQ=255)
                                                  tCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGt                                                                                            >  1:3362844/1‑148 (MQ=255)
                                                  tCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGt                                                                                            >  1:2607908/1‑148 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                        <  1:1438949/148‑1 (MQ=255)
                                                      tCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaa                                                                                        >  2:1271991/1‑148 (MQ=255)
                                                       cTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaaa                                                                                       <  2:2791075/148‑1 (MQ=255)
                                                       cTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGaaa                                                                                       >  2:1919777/1‑148 (MQ=255)
                                                         tCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACg                                                                                     <  1:2482066/148‑1 (MQ=255)
                                                           gcgTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGt                                                                                   <  1:2187152/148‑1 (MQ=255)
                                                              tAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTaag                                                                                >  1:1675713/1‑147 (MQ=255)
                                                               aGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAatt                                                                               <  2:3393697/148‑1 (MQ=255)
                                                               aGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAatt                                                                               <  2:176206/148‑1 (MQ=255)
                                                               aGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAatt                                                                               <  1:1363875/148‑1 (MQ=255)
                                                                ttgATGCGTTCTGGGGATACGCCTGGACAGTTAAGGAGCAATCTCTCCCGTAATCCGCCGGATTTTCTGATCAGCACAGCCTCATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAatta                                                                              <  2:889073/146‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGACACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                           <  1:68450/148‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                           <  1:2071983/148‑1 (MQ=255)
                                                                   aTCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtg                                                                           <  2:494239/148‑1 (MQ=255)
                                                                    tCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtga                                                                          >  1:384628/1‑148 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                       <  1:1978045/148‑1 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                       <  1:1008417/148‑1 (MQ=255)
                                                                       gTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATtgatga                                                                       <  1:1269210/148‑1 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                      >  1:651493/1‑148 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                      <  2:3433013/148‑1 (MQ=255)
                                                                        tACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGaa                                                                      <  1:3799919/148‑1 (MQ=255)
                                                                         aCTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAg                                                                     <  1:3368024/148‑1 (MQ=255)
                                                                           tGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTc                                                                   <  1:1149450/148‑1 (MQ=255)
                                                                           tGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTc                                                                   >  2:3075790/1‑148 (MQ=255)
                                                                           tGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTc                                                                   <  1:2858775/148‑1 (MQ=255)
                                                                            ggCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTcc                                                                  >  1:1649767/1‑148 (MQ=255)
                                                                            ggCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTcc                                                                  <  2:162651/148‑1 (MQ=255)
                                                                             gCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCa                                                                 <  2:287366/148‑1 (MQ=255)
                                                                             gCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCa                                                                 <  1:3086927/148‑1 (MQ=255)
                                                                                   cGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGt                                                           <  2:132280/148‑1 (MQ=255)
                                                                                    gCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTa                                                          >  1:965991/1‑148 (MQ=255)
                                                                                     ccTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAg                                                         >  2:208894/1‑148 (MQ=255)
                                                                                          acaGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGc                                                    <  2:2393839/148‑1 (MQ=255)
                                                                                             ggAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGt                                                 >  1:3071679/1‑148 (MQ=255)
                                                                                              gAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTa                                                >  1:1468808/1‑148 (MQ=255)
                                                                                              gAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTa                                                >  1:855147/1‑148 (MQ=255)
                                                                                              gAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTa                                                >  1:2769641/1‑148 (MQ=255)
                                                                                                 cGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAAc                                             >  2:3356244/1‑148 (MQ=255)
                                                                                                       aaaCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACgtggtg                                       >  2:780645/1‑148 (MQ=255)
                                                                                                        aaCTAACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTgc                                      >  2:3193715/1‑148 (MQ=255)
                                                                                                         aCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTc                                                                   <  2:2246762/118‑1 (MQ=255)
                                                                                                         aCTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTc                                                                   >  1:2246762/1‑118 (MQ=255)
                                                                                                          cTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTgcgc                                    <  1:2054159/148‑1 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                  <  1:1319669/148‑1 (MQ=255)
                                                                                                            cACCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCAt                                  >  2:1506951/1‑148 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                 <  1:208894/148‑1 (MQ=255)
                                                                                                             aCCCGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATc                                 >  2:1277810/1‑148 (MQ=255)
                                                                                                              cccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCt                                >  2:927407/1‑148 (MQ=255)
                                                                                                               ccGTAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTg                               >  1:2390822/1‑148 (MQ=255)
                                                                                                                  tAATCCGCCGGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGACACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCg                            <  1:80348/148‑1 (MQ=255)
                                                                                                                         ccgGATATTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTc                     >  2:1150314/1‑148 (MQ=255)
                                                                                                                              tatTCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAg                <  1:153526/148‑1 (MQ=255)
                                                                                                                                 tCTGATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCgg             >  2:522023/1‑148 (MQ=255)
                                                                                                                                    gATCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTc          >  2:1294181/1‑148 (MQ=255)
                                                                                                                                     aTCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCg         <  1:2815571/148‑1 (MQ=255)
                                                                                                                                     aTCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCg         >  1:3431385/1‑148 (MQ=255)
                                                                                                                                     aTCACCACACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCg         >  2:1033173/1‑148 (MQ=255)
                                                                                                                                       caccacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGAt       >  1:2983159/1‑148 (MQ=255)
                                                                                                                                        accacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATg      <  2:1468808/148‑1 (MQ=255)
                                                                                                                                        accacACCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATg      >  2:924850/1‑148 (MQ=255)
                                                                                                                                           acacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATgcgc   <  1:2199332/148‑1 (MQ=255)
                                                                                                                                            cacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgct  >  1:1596761/1‑148 (MQ=255)
                                                                                                                                            cacCCTAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCgct  >  1:1596250/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CCCGTTGAAGGGTATTGCCGATGAACGGCGGCGGCGCGGCGAAACGGAAGTCAATCTTCGCGTAGGGATCCGTACTGGCGATACGCCTGCACAGGAACGCAGCAAACTCACCCGTAATCCGCCGGATATTCTGATCACCACACCCGAATCACTCTATCTGATGCTGACCTCCCGCGCGCGCGAAACGCTACGCGGCGTCGAAACGGTAATTATTGATGAAGTCCACGCGGTAGCGGGCAGTAAACGTGGTGCGCATCTGGCGTTAAGTCTGGAGCGGCTCGATGCGCT  >  CP009273/1723664‑1723951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑