Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927312,9010GA99.6% 610.5 / ‑5.7 185D247N (GAT→AAT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (106/78);  minor base C (0/1);  total (106/79)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 191 total aligned reads displayed.

TACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12756‑13045
                                                                                                                                                 |                                                                                                                                                
tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAAt                                                                                                                                                >  2:1956693/1‑148 (MQ=255)
tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAAt                                                                                                                                                >  2:3413403/1‑148 (MQ=255)
tACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAAt                                                                                                                                                <  2:3541060/148‑1 (MQ=255)
 aCTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATc                                                                                                                                               <  2:855013/148‑1 (MQ=255)
  cTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCa                                                                                                                                              <  1:3665125/148‑1 (MQ=255)
   tttCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAg                                                                                                                                             >  2:2968533/1‑148 (MQ=255)
      cGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGc                                                                                                                                          <  1:2131305/148‑1 (MQ=255)
      cGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGc                                                                                                                                          >  1:3186557/1‑148 (MQ=255)
        atatTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCAt                                                                                                                                        >  1:821928/1‑148 (MQ=255)
            ttCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    >  1:3695327/1‑148 (MQ=255)
            ttCTAGTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGAc                                                                                                                                    <  2:3434562/148‑1 (MQ=255)
                attatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTgc                                                                                                                                >  1:1843715/1‑148 (MQ=255)
                attatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTgc                                                                                                                                >  2:249296/1‑148 (MQ=255)
                   atCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCa                                                                                                                             >  2:42264/1‑148 (MQ=255)
                   atCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCa                                                                                                                             >  1:685952/1‑148 (MQ=255)
                        aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGAt                                                                                                                        >  1:1453435/1‑148 (MQ=255)
                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATc                                                                                                                       >  1:3644534/1‑148 (MQ=255)
                         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATc                                                                                                                       >  2:1401420/1‑148 (MQ=255)
                           cgacgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCg                                                                                                                     <  2:687494/148‑1 (MQ=255)
                             acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCt                                                                                                                   >  1:259297/1‑148 (MQ=255)
                             acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCt                                                                                                                   >  1:3459320/1‑148 (MQ=255)
                             acgaAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCt                                                                                                                   >  1:3459307/1‑148 (MQ=255)
                                 aGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCa                                                                                                               >  1:2354220/1‑148 (MQ=255)
                                       cGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAg                                                                                                         <  1:1143713/148‑1 (MQ=255)
                                          cGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAgcgc                                                                                                      <  1:1048994/148‑1 (MQ=255)
                                           gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGcc                                                                                                     <  2:2573916/148‑1 (MQ=255)
                                           gAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGcc                                                                                                     <  2:1033386/148‑1 (MQ=255)
                                           gAAAAAACCTTCGAAGGTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGcc                                                                                                     <  2:1153599/148‑1 (MQ=255)
                                               aaaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaa                                                                                                 >  1:3338155/1‑148 (MQ=255)
                                                aaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaaa                                                                                                <  1:769205/148‑1 (MQ=255)
                                                aaCCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGaaa                                                                                                >  1:1542413/1‑148 (MQ=255)
                                                  ccTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaaga                                                                                              <  1:336826/148‑1 (MQ=255)
                                                    ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaag                                                                                            >  1:3134863/1‑148 (MQ=255)
                                                    ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaag                                                                                            >  1:3633480/1‑148 (MQ=255)
                                                    ttCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAaagaag                                                                                            >  1:1230831/1‑148 (MQ=255)
                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCACTGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                    >  2:3761128/1‑148 (MQ=255)
                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                    >  1:439316/1‑148 (MQ=255)
                                                            tCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaa                                                                                    >  1:3353662/1‑148 (MQ=255)
                                                               ggCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                 <  1:1027476/148‑1 (MQ=255)
                                                               ggCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                 <  2:20359/148‑1 (MQ=255)
                                                               ggCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGaaaaa                                                                                 >  2:3566693/1‑148 (MQ=255)
                                                                    ccaacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaa                                                                            >  1:2512779/1‑148 (MQ=255)
                                                                      aacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaaa                                                                          <  2:208383/148‑1 (MQ=255)
                                                                      aacGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGaaaa                                                                          <  1:1686894/148‑1 (MQ=255)
                                                                       acGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAAt                                                                         >  1:1185257/1‑148 (MQ=255)
                                                                       acGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAAt                                                                         <  2:3807198/148‑1 (MQ=255)
                                                                         ggTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCg                                                                       <  1:2471521/148‑1 (MQ=255)
                                                                          gTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGa                                                                      <  1:1008958/148‑1 (MQ=255)
                                                                          gTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGa                                                                      <  1:927049/148‑1 (MQ=255)
                                                                           tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGACGCGGCAGAAAAAGCGAAAATCGaa                                                                     <  2:1804318/148‑1 (MQ=255)
                                                                           tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGaa                                                                     <  1:2810118/148‑1 (MQ=255)
                                                                           tGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGaa                                                                     <  2:458208/148‑1 (MQ=255)
                                                                              tACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTg                                                                  <  2:3463857/148‑1 (MQ=255)
                                                                                cccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTc                                                                <  2:3549922/148‑1 (MQ=255)
                                                                                cccACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTc                                                                <  2:3792871/148‑1 (MQ=255)
                                                                                   aCCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTc                                                             <  2:2280371/148‑1 (MQ=255)
                                                                                   aCCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTc                                                             <  1:3269285/148‑1 (MQ=255)
                                                                                           ggTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTcagc                                                     >  1:1366357/1‑148 (MQ=255)
                                                                                               aaGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAc                                                 <  2:1981761/148‑1 (MQ=255)
                                                                                                aGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGAcc                                                >  2:3260210/1‑148 (MQ=255)
                                                                                                 gACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCg                                               >  1:2883639/1‑148 (MQ=255)
                                                                                                  aCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGa                                              >  1:2238652/1‑148 (MQ=255)
                                                                                                  aCTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGa                                              >  1:3295759/1‑148 (MQ=255)
                                                                                                    ttCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACg                                            <  1:3238548/148‑1 (MQ=255)
                                                                                                         cAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAc                                       <  1:2440911/148‑1 (MQ=255)
                                                                                                         cAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAc                                       >  1:110128/1‑148 (MQ=255)
                                                                                                          aGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAAcc                                      >  2:1026126/1‑148 (MQ=255)
                                                                                                            ccGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTg                                    >  1:2266273/1‑148 (MQ=255)
                                                                                                            ccGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTg                                    >  1:2375835/1‑148 (MQ=255)
                                                                                                             cGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGc                                   <  1:529392/148‑1 (MQ=255)
                                                                                                               tCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCTGCAGACCGACGTTAACCTGCCa                                 >  2:3204625/1‑148 (MQ=255)
                                                                                                               tCTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCa                                 >  2:825255/1‑148 (MQ=255)
                                                                                                                cTGATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCAt                                <  1:159180/148‑1 (MQ=255)
                                                                                                                  gATCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACATGCGCAACGATCCGCTGGCAATGAAGCCCCTGAAAGAAGCGGCAGAAAAAGAGAAAATCGAACTGTCTACCGCTCAGCAGACCTACGTTAACCTGCCATAc                              >  1:2691221/1‑148 (MQ=255)
                                                                                                                   aTCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACa                             >  2:221480/1‑148 (MQ=255)
                                                                                                                    tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            >  2:164993/1‑148 (MQ=255)
                                                                                                                    tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            >  1:177375/1‑148 (MQ=255)
                                                                                                                    tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            >  1:1980411/1‑148 (MQ=255)
                                                                                                                    tCAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACAt                            <  2:1905914/148‑1 (MQ=255)
                                                                                                                     cAACTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATc                           >  2:3649054/1‑148 (MQ=255)
                                                                                                                       aCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         >  1:3495751/1‑148 (MQ=255)
                                                                                                                       aCTATCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCAc                         >  2:3233654/1‑148 (MQ=255)
                                                                                                                       aCTAACTGGTTGAAGAATTCAAGAAAAATCAGGGCATAGACCAGCGCAACGATCCGCTGGCAAAGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCACAGCAGACCGACGTAAACCTGCCATACAacac                         >  2:491694/1‑148 (MQ=255)
                                                                                                                          aTCTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGc                      >  2:2074902/1‑148 (MQ=255)
                                                                                                                            cTGGTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAg                    <  1:1401420/148‑1 (MQ=255)
                                                                                                                              ggTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAc                  >  2:2133380/1‑148 (MQ=255)
                                                                                                                              ggTTGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAc                  >  1:1260992/1‑148 (MQ=255)
                                                                                                                                ttGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgc                >  1:3778705/1‑148 (MQ=255)
                                                                                                                                ttGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgc                >  2:306275/1‑148 (MQ=255)
                                                                                                                                 tGAAGAATTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGAcgcg               >  1:361883/1‑148 (MQ=255)
                                                                                                                                  gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGa              <  2:1502992/148‑1 (MQ=255)
                                                                                                                                  gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGa              <  2:1623408/148‑1 (MQ=255)
                                                                                                                                  gaagaaTTCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCGGGCAATGCAGCTCCTGAAAGTAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCTCTGCAGACGCGa              <  2:2410758/148‑1 (MQ=255)
                                                                                                                                       aTTCAAGAAAAATCAGGGCATTGAGCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGt         <  2:3695327/148‑1 (MQ=255)
                                                                                                                                        ttCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTc        >  2:3537961/1‑148 (MQ=255)
                                                                                                                                         tCAAGAAAAATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTcc       >  2:2116276/1‑148 (MQ=255)
                                                                                                                                             gaaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGaaa   >  2:295617/1‑148 (MQ=255)
                                                                                                                                              aaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAac  >  1:1497208/1‑148 (MQ=255)
                                                                                                                                              aaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAac  >  1:3045386/1‑148 (MQ=255)
                                                                                                                                              aaaaaTCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAac  >  1:1497784/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAAC  >  CP009273/12756‑13045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑