Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,769,763 T→A 100% intergenic (+62/‑81) aroD → / → ydiF 3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,769,7630TA95.8% 764.2 / 17.0 240intergenic (+62/‑81)aroD/ydiF3‑dehydroquinate dehydratase/putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
Reads supporting (aligned to +/- strand):  ref base T (3/7);  new base A (102/128);  total (105/135)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 244 total aligned reads displayed.

GGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769618‑1769907
                                                                                                                                                 |                                                                                                                                                
ggTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaa                                                                                                                                                <  2:1937919/148‑1 (MQ=255)
ggTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTaaa                                                                                                                                                <  2:2716597/148‑1 (MQ=255)
    agttAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATaaa                                                                                                                                            <  2:404859/145‑1 (MQ=255)
     ggTAAAAATAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATaaaa                                                                                                                                           <  2:948322/148‑1 (MQ=255)
        aaaaaaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAata                                                                                                                                        <  1:1200614/148‑1 (MQ=255)
            aaaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTc                                                                                                                                    >  1:3053467/1‑148 (MQ=255)
             aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCg                                                                                                                                   <  2:1655982/148‑1 (MQ=255)
             aaGCGTCTGCGCCAGGGCAAATCTCGGTAAATGAGTTGCGCACGGTATTAACTATTGTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCg                                                                                                                                   <  2:1410529/148‑1 (MQ=255)
                 gTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAg                                                                                                                               <  2:1480229/148‑1 (MQ=255)
                  tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGt                                                                                                                              <  2:98233/148‑1 (MQ=255)
                  tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGt                                                                                                                              <  1:1547632/148‑1 (MQ=255)
                  tCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGt                                                                                                                              <  2:1842179/148‑1 (MQ=255)
                     gcgcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGtctc                                                                                                                           <  1:3212531/148‑1 (MQ=255)
                       gcCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCtg                                                                                                                         <  2:343941/148‑1 (MQ=255)
                            ggCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAAGCGTATTAAATAAAAATATTTCGTGAGTCTCTGTgcgc                                                                                                                    >  2:2722120/1‑148 (MQ=255)
                              cAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTa                                                                                                                  <  1:514216/148‑1 (MQ=255)
                               aaaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTaa                                                                                                                 >  1:3218960/1‑148 (MQ=255)
                                aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAt                                                                                                                >  2:748049/1‑148 (MQ=255)
                                aaTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAt                                                                                                                >  1:3408295/1‑148 (MQ=255)
                                 aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                               <  2:3765848/148‑1 (MQ=255)
                                 aTCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAtt                                                                                                               <  1:3748715/148‑1 (MQ=255)
                                     cGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTcc                                                                                                           >  1:1651203/1‑148 (MQ=255)
                                      ggTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCa                                                                                                          <  2:2190291/148‑1 (MQ=255)
                                      ggTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCa                                                                                                          <  2:3190653/148‑1 (MQ=255)
                                         aaaTGATTTGCGCACGGTATTGACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       >  2:1029038/1‑148 (MQ=255)
                                         aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       <  2:886503/148‑1 (MQ=255)
                                         aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       >  2:1160357/1‑148 (MQ=255)
                                         aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAttt                                                                                                       >  2:2937383/1‑148 (MQ=255)
                                         aaaTGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCAgtt                                                                                                       >  2:1160219/1‑148 (MQ=255)
                                             gATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTGATTCTCCATTTGGCg                                                                                                   >  2:2956579/1‑148 (MQ=255)
                                             gATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCg                                                                                                   >  2:3003374/1‑148 (MQ=255)
                                              aTTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGt                                                                                                  >  1:1619325/1‑148 (MQ=255)
                                                      aCGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaaa                                                                                          <  1:1010324/148‑1 (MQ=255)
                                                      aCGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGaaaa                                                                                          <  1:2963901/148‑1 (MQ=255)
                                                        ggTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATc                                                                                        <  1:2741845/148‑1 (MQ=255)
                                                           aTTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcaca                                                                                     <  2:2896087/148‑1 (MQ=255)
                                                           aTTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATcaca                                                                                     <  2:710252/148‑1 (MQ=255)
                                                             tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                   >  2:1578402/1‑148 (MQ=255)
                                                             tAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATc                                                                                   <  2:712448/148‑1 (MQ=255)
                                                                  aTTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAAt                                                                              <  2:2287072/148‑1 (MQ=255)
                                                                   ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTTGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                             >  2:2472292/1‑148 (MQ=255)
                                                                   ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                             <  1:441001/148‑1 (MQ=255)
                                                                   ttttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATc                                                                             >  2:110520/1‑148 (MQ=255)
                                                                    tttACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCa                                                                            >  2:3128656/1‑148 (MQ=255)
                                                                      tACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAgg                                                                          <  2:2648622/148‑1 (MQ=255)
                                                                        cacCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGaa                                                                        <  1:1835692/148‑1 (MQ=255)
                                                                           cAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTa                                                                     <  1:600832/148‑1 (MQ=255)
                                                                            aGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTaa                                                                    <  2:2554031/148‑1 (MQ=255)
                                                                            aGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTaa                                                                    <  2:3013260/148‑1 (MQ=255)
                                                                              gCATAATCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACa                                                                  <  2:1690779/148‑1 (MQ=255)
                                                                              gCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACa                                                                  >  2:2783999/1‑148 (MQ=255)
                                                                              gCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACa                                                                  <  1:1425783/148‑1 (MQ=255)
                                                                                aTAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAAt                                                                <  1:499257/148‑1 (MQ=255)
                                                                                 tAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATg                                                               >  2:1278252/1‑148 (MQ=255)
                                                                                    gCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGaaa                                                            <  2:2888705/148‑1 (MQ=255)
                                                                                      aataatATTTCGGCGGGAACACCCTCACCGCATAACTAAAAAATATATTCAATCTTATTTAATAAAAATATTTCGTGATTCTCTGTGCGCTAATTATCCATTTGGCGTAGGGAAAATCACATATTAATCAGGAATTAACAATGAAAcc                                                          <  2:3615991/148‑1 (MQ=255)
                                                                                       ataataTTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCt                                                         >  2:3565348/1‑148 (MQ=255)
                                                                                           tatTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaa                                                     >  1:3281988/1‑148 (MQ=255)
                                                                                            atTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTaaa                                                    >  1:1676210/1‑148 (MQ=255)
                                                                                              ttCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAac                                                  >  2:2093504/1‑148 (MQ=255)
                                                                                               tCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAacc                                                 <  1:2811858/148‑1 (MQ=255)
                                                                                                      gAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGt                                          <  1:856128/148‑1 (MQ=255)
                                                                                                       aaCACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTa                                         >  2:1404185/1‑148 (MQ=255)
                                                                                                       aaCACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTa                                         >  1:3572857/1‑148 (MQ=255)
                                                                                                          acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                      <  1:1318300/148‑1 (MQ=255)
                                                                                                          acCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTa                                      <  1:706753/148‑1 (MQ=255)
                                                                                                           cccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTaa                                     <  2:479301/148‑1 (MQ=255)
                                                                                                           cccTCCCCGCCGAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTaa                                     >  1:1941749/1‑148 (MQ=255)
                                                                                                                  cgccgAAATAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGa                              <  2:3462455/148‑1 (MQ=255)
                                                                                                                     cgAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTg                           <  1:1499555/148‑1 (MQ=255)
                                                                                                                      gAACTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGc                          <  2:2920090/148‑1 (MQ=255)
                                                                                                                         cTAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCgg                       >  1:2490164/1‑148 (MQ=255)
                                                                                                                          tAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGt                      >  2:3363164/1‑148 (MQ=255)
                                                                                                                          tAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGt                      <  2:3531478/148‑1 (MQ=255)
                                                                                                                          tAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGt                      >  2:1517564/1‑148 (MQ=255)
                                                                                                                          tAAAAAATATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGt                      >  2:1460566/1‑148 (MQ=255)
                                                                                                                           aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                     >  1:2889578/1‑148 (MQ=255)
                                                                                                                           aaaaaaTATATTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTc                     <  1:3128656/148‑1 (MQ=255)
                                                                                                                              aaaTATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTg                  >  1:3377316/1‑148 (MQ=255)
                                                                                                                                atatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                >  1:3637785/1‑148 (MQ=255)
                                                                                                                                atatatTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTc                >  1:93255/1‑148 (MQ=255)
                                                                                                                                  atatTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCgg              >  2:2637165/1‑148 (MQ=255)
                                                                                                                                    atTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca            <  1:1134234/148‑1 (MQ=255)
                                                                                                                                    atTCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGca            >  2:250559/1‑148 (MQ=255)
                                                                                                                                      tCAATCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGcaca          <  1:2783999/148‑1 (MQ=255)
                                                                                                                                         aTCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGa       >  1:1753725/1‑148 (MQ=255)
                                                                                                                                          tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa      >  1:3744981/1‑148 (MQ=255)
                                                                                                                                          tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa      >  1:1704384/1‑148 (MQ=255)
                                                                                                                                          tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGaa      >  2:2432340/1‑148 (MQ=255)
                                                                                                                                          tCGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATAAACAATGAAACCTGTAAAACCACCTCTTATTAATGGACGAGTGCCGTTCCTGTCGGCACAGGaa      >  1:1484800/1‑148 (MQ=255)
                                                                                                                                           cGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAg     <  1:2937383/148‑1 (MQ=255)
                                                                                                                                           cGTATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAg     <  1:1029038/148‑1 (MQ=255)
                                                                                                                                             tATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg   <  1:748049/148‑1 (MQ=255)
                                                                                                                                             tATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg   >  1:1402145/1‑148 (MQ=255)
                                                                                                                                             tATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg   >  1:203755/1‑148 (MQ=255)
                                                                                                                                             tATTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAAGTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCg   <  2:469871/148‑1 (MQ=255)
                                                                                                                                             tATTAAATAAAAATAATTCGTGAGTCTCTGTGCGCTAAGTCGCCATTTGGAGTAGGGAAAATCACATCTGACTCAGGAATAAACACTGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCGGTCGGCACAGGAcgcg   <  2:1007943/148‑1 (MQ=255)
                                                                                                                                              aTTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  <  2:2207923/148‑1 (MQ=255)
                                                                                                                                              aTTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  <  2:1863044/148‑1 (MQ=255)
                                                                                                                                              aTTAAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCgg  >  1:199391/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTGCGGTAAAAAAAGCGTCTGCGCCAGGGCAAATCTCGGTAAATGATTTGCGCACGGTATTAACTATTTTACACCAGGCATAAGCAATAATATTTCGGCGGGAACACCCTCCCCGCCGAACTAAAAAATATATTCAATCGTATTTAATAAAAATATTTCGTGAGTCTCTGTGCGCTAATTCTCCATTTGGCGTAGGGAAAATCACATCTGAATCAGGAATTAACAATGAAACCTGTAAAACCACCTCGTATTAATGGACGAGTGCCGGTCCTGTCGGCACAGGAAGCGG  >  CP009273/1769618‑1769907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑