Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,800,129 C→T 100% W71* (TGG→TAG)  ydiY ← acid‑inducible putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,800,1290CT99.5% 683.9 / ‑4.4 210W71* (TGG→TAG) ydiYacid‑inducible putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (111/97);  total (113/97)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGC  >  CP009273/1799985‑1800271
                                                                                                                                                |                                                                                                                                              
cTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAgg                                                                                                                                             >  1:3779507/1‑148 (MQ=255)
  tGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTc                                                                                                                                           >  1:1205256/1‑148 (MQ=255)
  tGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTc                                                                                                                                           >  2:274962/1‑148 (MQ=255)
             ataaataGTCATAATCAGTTAAATTAAAAAGGCTACGCCCGCCCGCCGCAAATTTTTCTGAATAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTc                                                                                                                                >  2:3735474/1‑148 (MQ=255)
              taaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCa                                                                                                                               >  2:647372/1‑148 (MQ=255)
              taaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCa                                                                                                                               >  2:116618/1‑148 (MQ=255)
                 ataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGcc                                                                                                                            >  1:3093235/1‑148 (MQ=255)
                 ataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGcc                                                                                                                            >  1:3093183/1‑148 (MQ=255)
                 ataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGcc                                                                                                                            >  2:1535293/1‑148 (MQ=255)
                   aGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGt                                                                                                                          >  1:605452/1‑148 (MQ=255)
                   aGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGt                                                                                                                          >  1:828408/1‑148 (MQ=255)
                   aGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTAATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGTTCATGGTGGTGTCAgacta                                                                                                                          >  2:209680/1‑144 (MQ=255)
                     tCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTaa                                                                                                                        >  1:1090014/1‑148 (MQ=255)
                     tCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTaa                                                                                                                        >  2:2236661/1‑148 (MQ=255)
                     tCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTaa                                                                                                                        >  2:2901314/1‑148 (MQ=255)
                      cATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAg                                                                                                                       >  2:2320631/1‑148 (MQ=255)
                       aTAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGt                                                                                                                      >  2:2587458/1‑148 (MQ=255)
                       aTAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGt                                                                                                                      >  2:3268144/1‑148 (MQ=255)
                          aTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgag                                                                                                                   >  1:2260318/1‑148 (MQ=255)
                            cAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgagga                                                                                                                 >  2:2547886/1‑148 (MQ=255)
                            cAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgagga                                                                                                                 >  2:630378/1‑148 (MQ=255)
                             aGTTAAATAAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgaggag                                                                                                                <  1:495004/148‑1 (MQ=255)
                               ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCt                                                                                                              <  2:2290737/148‑1 (MQ=255)
                               ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCt                                                                                                              >  2:1955205/1‑148 (MQ=255)
                               ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCt                                                                                                              <  2:296078/148‑1 (MQ=255)
                               ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCt                                                                                                              >  1:3159440/1‑148 (MQ=255)
                                 aaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCttt                                                                                                            >  2:2073635/1‑148 (MQ=255)
                                 aaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCttt                                                                                                            <  2:187056/148‑1 (MQ=255)
                                 aaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCttt                                                                                                            <  2:1295478/148‑1 (MQ=255)
                                 aaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCttt                                                                                                            >  1:2757690/1‑148 (MQ=255)
                                  aattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCtttt                                                                                                           <  2:3509435/148‑1 (MQ=255)
                                    ttaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCg                                                                                                         >  1:1432479/1‑148 (MQ=255)
                                     taaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGt                                                                                                        <  1:3775219/148‑1 (MQ=255)
                                       aaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAt                                                                                                      >  2:148846/1‑148 (MQ=255)
                                       aaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAt                                                                                                      >  1:1663452/1‑148 (MQ=255)
                                       aaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAt                                                                                                      <  2:2657727/148‑1 (MQ=255)
                                        aaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAtt                                                                                                     >  1:1207510/1‑148 (MQ=255)
                                         aCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACGGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTg                                                                                                    <  1:1472468/148‑1 (MQ=255)
                                          cGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGc                                                                                                   >  1:1109358/1‑148 (MQ=255)
                                             cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTg                                                                                                <  2:605452/148‑1 (MQ=255)
                                             cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTg                                                                                                >  1:3007727/1‑148 (MQ=255)
                                             cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTg                                                                                                <  2:828408/148‑1 (MQ=255)
                                                 gcccgcccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATtg                                                                                            >  2:2489472/1‑148 (MQ=255)
                                                     gcccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTTTTGCCTGATTGTGCa                                                                                        <  1:1523053/148‑1 (MQ=255)
                                                       ccgccgCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAg                                                                                      <  2:741538/148‑1 (MQ=255)
                                                       ccgccgCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAg                                                                                      <  1:2082400/148‑1 (MQ=255)
                                                        cgccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGa                                                                                     >  1:3626928/1‑148 (MQ=255)
                                                         gccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                    <  1:3280431/148‑1 (MQ=255)
                                                          ccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGata                                                                                   >  1:951196/1‑148 (MQ=255)
                                                            gcATATTTTTCTGAAGAACGATCATCATTAGAAGAAGTATTTCTGGCGTTCCCCGACAGCGACGAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTGAGCCGTATGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATc                                                                                 <  1:1907008/148‑1 (MQ=255)
                                                              atatTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCt                                                                               >  2:859579/1‑148 (MQ=255)
                                                               tatTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTg                                                                              >  2:3706305/1‑148 (MQ=255)
                                                                atTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGc                                                                             >  2:1463410/1‑148 (MQ=255)
                                                                atTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGc                                                                             >  1:772334/1‑148 (MQ=255)
                                                                   tttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGTGAACAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGtt                                                                          <  1:3410581/148‑1 (MQ=255)
                                                                   tttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGtt                                                                          <  2:486918/148‑1 (MQ=255)
                                                                    ttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTc                                                                         <  2:2261663/148‑1 (MQ=255)
                                                                      cTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAg                                                                       >  1:2528961/1‑148 (MQ=255)
                                                                        gaagaaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGAt                                                                     <  2:3224844/148‑1 (MQ=255)
                                                                           gaaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAc                                                                  >  2:1403622/1‑148 (MQ=255)
                                                                           gaaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAc                                                                  <  2:514732/148‑1 (MQ=255)
                                                                                  catcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGaa                                                           <  2:3093189/148‑1 (MQ=255)
                                                                                  catcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGaa                                                           <  2:3093143/148‑1 (MQ=255)
                                                                                  catcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGGTCAGATTACCTTCGaa                                                           >  1:746985/1‑148 (MQ=255)
                                                                                  catcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAACCTGCGTTCAGATTACCTTCGaa                                                           >  1:1631526/1‑148 (MQ=255)
                                                                                    tcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAg                                                         >  1:2050329/1‑148 (MQ=255)
                                                                                    tcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAg                                                         <  2:2784376/148‑1 (MQ=255)
                                                                                    tcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAg                                                         >  1:136874/1‑148 (MQ=255)
                                                                                     catTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAgg                                                        <  2:1707340/148‑1 (MQ=255)
                                                                                     catTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAgg                                                        <  2:1706588/148‑1 (MQ=255)
                                                                                     catTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAgg                                                        >  1:1030700/1‑148 (MQ=255)
                                                                                        tAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGttt                                                     >  2:3622180/1‑148 (MQ=255)
                                                                                          gaagaaGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGttttt                                                   <  2:570163/148‑1 (MQ=255)
                                                                                           aagaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtttttt                                                  >  1:2748204/1‑148 (MQ=255)
                                                                                           aagaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtttttt                                                  >  1:3462889/1‑148 (MQ=255)
                                                                                            agaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCATCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTa                                                 <  2:299668/148‑1 (MQ=255)
                                                                                            agaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTa                                                 <  2:2927612/148‑1 (MQ=255)
                                                                                               agTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAgcg                                              <  1:913449/148‑1 (MQ=255)
                                                                                                        ggCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                     >  2:2421262/1‑148 (MQ=255)
                                                                                                          cGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAATTTCGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatc                                   <  1:3735474/148‑1 (MQ=255)
                                                                                                             tCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatcatc                                >  1:854970/1‑148 (MQ=255)
                                                                                                             tCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatcatc                                >  1:343278/1‑148 (MQ=255)
                                                                                                              ccccccCAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATcc                               <  1:378937/148‑1 (MQ=255)
                                                                                                              cccccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATcc                               <  1:116618/148‑1 (MQ=255)
                                                                                                                 ccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATg                            <  1:1598846/148‑1 (MQ=255)
                                                                                                                   acaGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGAc                          >  1:412924/1‑148 (MQ=255)
                                                                                                                       cGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTa                      >  2:2520230/1‑148 (MQ=255)
                                                                                                                         aCCAGGCAGTGGTGTGTCCATACAAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTaaa                    >  1:3217615/1‑148 (MQ=255)
                                                                                                                            aGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAAc                 <  1:647372/148‑1 (MQ=255)
                                                                                                                             ggCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACg                >  1:2408725/1‑148 (MQ=255)
                                                                                                                               cAGTGTTGTGTCAATACTAGGTTATGGTGGTGTCAGCAGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGAAAGATATCCTGCGTTCAGATTAACTTCGAAATGTTTTTTAGCGCTGGCAGGTTCATCCATGACAGTAAAAACGGa              <  2:3785223/148‑1 (MQ=255)
                                                                                                                               cAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGa              <  2:1893896/148‑1 (MQ=255)
                                                                                                                                aGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa             <  2:1663452/148‑1 (MQ=255)
                                                                                                                                aGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa             <  2:1336188/148‑1 (MQ=255)
                                                                                                                                 gtggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAat            >  1:1405017/1‑148 (MQ=255)
                                                                                                                                    gtgtgtCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatcat         >  2:3307682/1‑148 (MQ=255)
                                                                                                                                     tgtgtCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatcatc        >  1:2095243/1‑148 (MQ=255)
                                                                                                                                        gtCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGc     <  1:2865955/148‑1 (MQ=255)
                                                                                                                                        gtCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGc     <  2:1659738/148‑1 (MQ=255)
                                                                                                                                          ccATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAg   <  1:1785241/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGC  >  CP009273/1799985‑1800271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑