Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,803,109 C→T 100% A128T (GCC→ACC)  yniB ← putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,803,1090CT95.3% 820.2 / 22.8 257A128T (GCC→ACC) yniBputative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (6/6);  new base T (129/116);  total (135/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 261 total aligned reads displayed.

CCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAA  >  CP009273/1802964‑1803253
                                                                                                                                                 |                                                                                                                                                
 cAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATCTACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGtttt                                                                                                                                               >  2:3265711/1‑148 (MQ=255)
  aaGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCtttt                                                                                                                                              >  1:2370013/1‑148 (MQ=255)
    gCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTc                                                                                                                                            >  2:156667/1‑148 (MQ=255)
    gCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTc                                                                                                                                            >  2:156894/1‑148 (MQ=255)
     cAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTcc                                                                                                                                           >  1:319718/1‑148 (MQ=255)
       gagaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAg                                                                                                                                         >  1:1013269/1‑148 (MQ=255)
       gagaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAg                                                                                                                                         >  1:3419617/1‑148 (MQ=255)
       gagaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAg                                                                                                                                         >  1:2457992/1‑148 (MQ=255)
        agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGa                                                                                                                                        >  2:3377924/1‑148 (MQ=255)
        agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGa                                                                                                                                        >  2:3153877/1‑148 (MQ=255)
        agaAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGa                                                                                                                                        <  1:3067305/148‑1 (MQ=255)
          aaaaGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAAt                                                                                                                                      >  1:2065514/1‑148 (MQ=255)
             aGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAg                                                                                                                                   >  2:2139975/1‑148 (MQ=255)
              gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGt                                                                                                                                  >  2:1600659/1‑148 (MQ=255)
              gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGt                                                                                                                                  <  1:830800/148‑1 (MQ=255)
              gAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGt                                                                                                                                  <  2:1611769/148‑1 (MQ=255)
                acacATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAACATAGTATGGTGCGGAACAACAATACGGGACTCAAGTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTg                                                                                                                                >  2:1964915/1‑148 (MQ=255)
                acacATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTg                                                                                                                                >  1:717989/1‑148 (MQ=255)
                   caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATc                                                                                                                             <  1:3349279/148‑1 (MQ=255)
                   caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATc                                                                                                                             <  2:1980544/148‑1 (MQ=255)
                   caTAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATc                                                                                                                             >  1:116384/1‑148 (MQ=255)
                        ccTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCaa                                                                                                                        >  2:2314678/1‑148 (MQ=255)
                        ccTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCaa                                                                                                                        >  1:3373234/1‑148 (MQ=255)
                         cTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAac                                                                                                                       >  1:2606791/1‑148 (MQ=255)
                         cTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAac                                                                                                                       <  1:1117255/148‑1 (MQ=255)
                           gcagcaATAAAAATAACCGGCAGTATATACAGTTAGAAAAACTGAAGTAAAATAGTTTGGTGCTTAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTTGTTTTTTCCAGAATGAGTTGATCTTCAacac                                                                                                                     <  1:2130863/148‑1 (MQ=255)
                            cagcaATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACAcc                                                                                                                    <  1:40285/148‑1 (MQ=255)
                               caATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTc                                                                                                                 <  1:1358978/148‑1 (MQ=255)
                                 aTACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTccc                                                                                                               >  2:78292/1‑148 (MQ=255)
                                    cAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCa                                                                                                            <  1:2515376/148‑1 (MQ=255)
                                       aTAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaaga                                                                                                         >  2:1153018/1‑148 (MQ=255)
                                        tAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                                                                                        >  2:2328168/1‑148 (MQ=255)
                                        tAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaa                                                                                                        >  2:152980/1‑148 (MQ=255)
                                         aaCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCaagaag                                                                                                       >  1:95119/1‑148 (MQ=255)
                                          aCCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTGCACGCGTTAGCCCCTCTTCCCCGTTGGTTTTTTCCAGAATGAGTAGATCTTCAACACCTTCCCGCAAGAAgc                                                                                                      >  2:2254588/1‑148 (MQ=255)
                                              gCAGGATATTCATTGATAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTATCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc                                                                                                  <  1:1244536/148‑1 (MQ=255)
                                              gCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAgcgcgc                                                                                                  <  1:1744493/148‑1 (MQ=255)
                                               cAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGcc                                                                                                 <  2:3035557/148‑1 (MQ=255)
                                                  gATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                              >  1:3237356/1‑148 (MQ=255)
                                                  gATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTgg                                                                                              >  1:480330/1‑148 (MQ=255)
                                                     ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                           <  2:1462404/148‑1 (MQ=255)
                                                     ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                           >  1:1076738/1‑148 (MQ=255)
                                                     ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                           <  1:309793/148‑1 (MQ=255)
                                                     ataCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGa                                                                                           >  1:2419898/1‑148 (MQ=255)
                                                       aCAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACt                                                                                         >  2:130563/1‑148 (MQ=255)
                                                          gTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCAt                                                                                      <  1:105810/148‑1 (MQ=255)
                                                            gagaAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATcc                                                                                    >  1:3284408/1‑148 (MQ=255)
                                                                aaaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGc                                                                                >  1:2839152/1‑148 (MQ=255)
                                                                 aaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                               >  2:902028/1‑148 (MQ=255)
                                                                 aaaCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCa                                                                               >  2:1052902/1‑148 (MQ=255)
                                                                   aCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAcc                                                                             >  2:1978345/1‑148 (MQ=255)
                                                                   aCTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCAcc                                                                             >  1:2374150/1‑148 (MQ=255)
                                                                    cTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCg                                                                            >  1:647933/1‑148 (MQ=255)
                                                                    cTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCg                                                                            >  2:3322502/1‑148 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                       <  2:717989/148‑1 (MQ=255)
                                                                         ggAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATc                                                                       <  1:686663/148‑1 (MQ=255)
                                                                          gAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATcc                                                                      >  1:3819404/1‑148 (MQ=255)
                                                                                  tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACTCGTTAGCCCCTCTTCCCCATTGGTTATTTCCAGAATGAGTTGATCTTCAACACCTTCACTCAAGAAGCGCGCCTGGCGACTCATCCTGGCAACCGAATCATGAAActc                                                              >  1:3436135/1‑146 (MQ=255)
                                                                                  tATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGc                                                              >  1:978156/1‑148 (MQ=255)
                                                                                   aTGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGcc                                                             >  1:610551/1‑148 (MQ=255)
                                                                                            aacaacAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACa                                                    >  1:309543/1‑148 (MQ=255)
                                                                                             acaacaATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaa                                                   <  2:3558146/148‑1 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                                <  2:2370171/148‑1 (MQ=255)
                                                                                                acaaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACaaaaa                                                <  2:2369710/148‑1 (MQ=255)
                                                                                                 caaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt                                               <  1:2327991/148‑1 (MQ=255)
                                                                                                  taTACGGGACTAAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:158207/147‑1 (MQ=255)
                                                                                                  aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:2790049/148‑1 (MQ=255)
                                                                                                  aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:458192/148‑1 (MQ=255)
                                                                                                  aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:2880786/148‑1 (MQ=255)
                                                                                                  aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:3218421/148‑1 (MQ=255)
                                                                                                  aaTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATc                                              <  2:1315530/148‑1 (MQ=255)
                                                                                                   aTACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCa                                             <  1:3403579/148‑1 (MQ=255)
                                                                                                      cGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                          >  1:3692243/1‑148 (MQ=255)
                                                                                                      cGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                          <  1:2136727/148‑1 (MQ=255)
                                                                                                      cGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGa                                          <  1:1285945/148‑1 (MQ=255)
                                                                                                       gggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGAt                                         <  1:2279782/148‑1 (MQ=255)
                                                                                                        ggACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATg                                        <  2:247236/148‑1 (MQ=255)
                                                                                                         gACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGa                                       >  2:2113992/1‑148 (MQ=255)
                                                                                                           cTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaa                                     <  1:3520931/148‑1 (MQ=255)
                                                                                                            tCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaaa                                    <  1:1600659/148‑1 (MQ=255)
                                                                                                                tttGTTCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCAc                                >  2:1253673/1‑148 (MQ=255)
                                                                                                                     tCACGCGTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGa                           <  2:3786586/148‑1 (MQ=255)
                                                                                                                        cgcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACa                        <  1:156667/148‑1 (MQ=255)
                                                                                                                        cgcgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACa                        <  1:2085149/148‑1 (MQ=255)
                                                                                                                          cgTTAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATa                      <  2:3799175/148‑1 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                   >  1:2940878/1‑148 (MQ=255)
                                                                                                                             tAGCCCCTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACg                   <  2:3335747/148‑1 (MQ=255)
                                                                                                                                ccccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTc                <  1:1149160/148‑1 (MQ=255)
                                                                                                                                ccccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTc                <  2:1407410/148‑1 (MQ=255)
                                                                                                                                ccccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTc                <  2:162543/148‑1 (MQ=255)
                                                                                                                                 cccTCTTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCa               <  2:3214963/148‑1 (MQ=255)
                                                                                                                                  ccTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCAc              >  2:3592414/1‑148 (MQ=255)
                                                                                                                                    tctTCCCCTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGc            <  2:2632224/148‑1 (MQ=255)
                                                                                                                                        ccccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacc        <  1:2653122/148‑1 (MQ=255)
                                                                                                                                         cccTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccacca       >  2:625339/1‑148 (MQ=255)
                                                                                                                                           cTTTGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAtt     >  1:300107/1‑148 (MQ=255)
                                                                                                                                            tttGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTa    >  2:1851822/1‑148 (MQ=255)
                                                                                                                                              tGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa  <  1:1964915/148‑1 (MQ=255)
                                                                                                                                              tGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa  <  1:1414617/148‑1 (MQ=255)
                                                                                                                                              tGGTTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaa  >  2:846580/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAAGCAGAGAAAAGAACACATAACCTGCAGCAATACAAATAACCGGCAGGATATACAGTGAGAAAAACTGCAGGAAAATAGTATGGTGCGGAACAACAATACGGGACTCAATTTGTTCACGCGTTAGCCCCTCTTCCCCTTTGGCTTTTTCCAGAATGAGTTGATCTTCAACACCTTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAA  >  CP009273/1802964‑1803253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑