Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,820,888 C→A 100% C98F (TGC→TTC)  astE ← succinylglutamate desuccinylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,820,8880CA98.8% 609.4 / ‑1.6 188C98F (TGC→TTC) astEsuccinylglutamate desuccinylase
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (103/82);  total (105/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 190 total aligned reads displayed.

TAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCC  >  CP009273/1820743‑1821031
                                                                                                                                                 |                                                                                                                                               
tAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGaaa                                                                                                                                               <  1:1115596/148‑1 (MQ=255)
  gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATa                                                                                                                                             >  1:2615616/1‑148 (MQ=255)
  gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATa                                                                                                                                             >  1:2688487/1‑148 (MQ=255)
  gATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATa                                                                                                                                             <  2:1585502/148‑1 (MQ=255)
   aTCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATaa                                                                                                                                            >  2:2723462/1‑148 (MQ=255)
     cAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                          >  1:3142769/1‑148 (MQ=255)
     cAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                          >  1:3143075/1‑148 (MQ=255)
     cAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACg                                                                                                                                          >  1:3541609/1‑148 (MQ=255)
      aaGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGt                                                                                                                                         >  2:1538980/1‑148 (MQ=255)
       aGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACttt                                                                                                                                        >  2:3074675/1‑148 (MQ=255)
        ggTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGttt                                                                                                                                       <  1:2676521/148‑1 (MQ=255)
         gTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTc                                                                                                                                      <  2:2343202/148‑1 (MQ=255)
          tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTcc                                                                                                                                     <  2:1407190/148‑1 (MQ=255)
          tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTcc                                                                                                                                     <  1:1212479/148‑1 (MQ=255)
          tGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTcc                                                                                                                                     <  2:802056/148‑1 (MQ=255)
           gCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTccc                                                                                                                                    <  1:3355612/148‑1 (MQ=255)
            cctgcgcACATATTCTTTGCCGTGTTAATAAAATTCTTCCTGGCAGTGTTCCTGTTCGCGCGCCCTACTGGTTTCCCCGATTTCCCAAAATAGCTCCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                   <  1:15058/145‑1 (MQ=255)
            ccAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCt                                                                                                                                   <  1:3718781/148‑1 (MQ=255)
              aGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTg                                                                                                                                 <  2:581399/148‑1 (MQ=255)
               gcgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGc                                                                                                                                <  2:3511414/148‑1 (MQ=255)
                cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                               >  2:3129726/1‑148 (MQ=255)
                cgcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCt                                                                                                                               <  1:3794568/148‑1 (MQ=255)
                 gcACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCtt                                                                                                                              <  1:1387859/148‑1 (MQ=255)
                  cacaGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTc                                                                                                                             <  2:3158496/148‑1 (MQ=255)
                   acaGATTCTTTGCCCTGGTCATAATAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATCGCTGCCAACGACCGCCCAACCTTCGATTCATATCGCTCTGGAAATAACGTTTCCCTTGCTTCa                                                                                                                            >  1:1330183/1‑148 (MQ=255)
                      gATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcg                                                                                                                         >  2:902593/1‑148 (MQ=255)
                       aTTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                        >  1:1873309/1‑148 (MQ=255)
                       aTTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                        >  2:3702065/1‑148 (MQ=255)
                       aTTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                        >  1:1076038/1‑148 (MQ=255)
                       aTTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAAAGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAgcgc                                                                                                                        >  2:3561583/1‑148 (MQ=255)
                          cTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCagg                                                                                                                     <  2:3143075/148‑1 (MQ=255)
                          cTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCagg                                                                                                                     <  2:3541609/148‑1 (MQ=255)
                          cTTTGCCCTGGTCATAAAAATCTGCCAGGCACTGTTCCAGTTCGCGCGCCCGACCGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCagg                                                                                                                     <  2:1626591/148‑1 (MQ=255)
                            ttGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCaggag                                                                                                                   >  1:6866/1‑148 (MQ=255)
                              gCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCTCAggagga                                                                                                                 <  2:2179575/148‑1 (MQ=255)
                              gCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGGTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAggagga                                                                                                                 <  2:2279180/148‑1 (MQ=255)
                                ccTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGAtt                                                                                                               <  2:2691867/148‑1 (MQ=255)
                                 cTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTc                                                                                                              >  2:1028458/1‑148 (MQ=255)
                                 cTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGATGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTc                                                                                                              >  1:3449251/1‑148 (MQ=255)
                                   ggTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTccc                                                                                                            >  1:2526150/1‑148 (MQ=255)
                                   ggTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTcca                                                                                                            >  2:3260312/1‑147 (MQ=255)
                                    gTCATAAAAATCTTCCCGGCACTGTTCCAGGTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCg                                                                                                           <  2:2091790/148‑1 (MQ=255)
                                    gTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCg                                                                                                           <  2:3701075/148‑1 (MQ=255)
                                     tCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa                                                                                                          >  1:1360322/1‑148 (MQ=255)
                                     tCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGa                                                                                                          <  1:802713/148‑1 (MQ=255)
                                         aaaaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                      >  1:2444483/1‑148 (MQ=255)
                                         aaaaaTCTACCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGAt                                                                                                      >  1:2130503/1‑148 (MQ=255)
                                           aaaTCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCa                                                                                                    >  2:2632855/1‑148 (MQ=255)
                                                    aGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAAcc                                                                                           >  1:57812/1‑148 (MQ=255)
                                                      gCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGc                                                                                         >  1:2780468/1‑148 (MQ=255)
                                                           gTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACg                                                                                    <  1:1634737/148‑1 (MQ=255)
                                                             tCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTa                                                                                  >  2:1246099/1‑148 (MQ=255)
                                                             tCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTa                                                                                  <  2:1803982/148‑1 (MQ=255)
                                                                aGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACg                                                                               >  2:2934898/1‑148 (MQ=255)
                                                                  ttCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                             >  1:846314/1‑148 (MQ=255)
                                                                  ttCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACggg                                                                             <  1:1892599/148‑1 (MQ=255)
                                                                              aCAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTga                                                                 >  2:1601584/1‑148 (MQ=255)
                                                                               cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                <  1:242334/148‑1 (MQ=255)
                                                                               cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                >  1:1146117/1‑148 (MQ=255)
                                                                               cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                <  1:242168/148‑1 (MQ=255)
                                                                               cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                >  2:818421/1‑148 (MQ=255)
                                                                               cAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgag                                                                >  1:3236703/1‑148 (MQ=255)
                                                                                aGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTgaga                                                               <  2:3449251/148‑1 (MQ=255)
                                                                                   tttCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGACAACGTAACGGGATCTCGCCGTGAGatat                                                            >  2:1440662/1‑148 (MQ=255)
                                                                                   tttCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCATTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGatat                                                            >  2:1415459/1‑148 (MQ=255)
                                                                                       tcCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcgcg                                                        >  2:2919822/1‑148 (MQ=255)
                                                                                       tcCGCTTTCAGCAAATAGCGGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATcgcg                                                        <  1:2566702/148‑1 (MQ=255)
                                                                                        ccGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCgcgc                                                       >  2:799631/1‑148 (MQ=255)
                                                                                        ccGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCgcgc                                                       <  2:3321217/148‑1 (MQ=255)
                                                                                        ccGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCgcgc                                                       <  1:238560/148‑1 (MQ=255)
                                                                                            tttCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAg                                                   <  2:255579/148‑1 (MQ=255)
                                                                                               cAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCaa                                                <  1:1696188/148‑1 (MQ=255)
                                                                                               cAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCaa                                                <  1:1709251/148‑1 (MQ=255)
                                                                                                    aaTAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGt                                           <  2:6866/148‑1 (MQ=255)
                                                                                                              aaCGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtctc                                 <  1:3129726/148‑1 (MQ=255)
                                                                                                              aaCGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCAtctc                                 >  2:3153632/1‑148 (MQ=255)
                                                                                                               aCGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTcc                                >  2:1805977/1‑148 (MQ=255)
                                                                                                                cGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCca                               >  2:626417/1‑148 (MQ=255)
                                                                                                                cGACCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCca                               >  2:2083617/1‑148 (MQ=255)
                                                                                                                  aCCGCCAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCcaca                             <  2:1636010/148‑1 (MQ=255)
                                                                                                                      ccAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTg                         <  2:1750390/148‑1 (MQ=255)
                                                                                                                      ccAAACATTCAATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTg                         >  1:1687766/1‑148 (MQ=255)
                                                                                                                       cAAACATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGc                        <  2:1775980/148‑1 (MQ=255)
                                                                                                                        aaaCATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGcc                       >  1:454941/1‑148 (MQ=255)
                                                                                                                          aCATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGt                     >  2:3770065/1‑148 (MQ=255)
                                                                                                                           cATTCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtc                    >  2:3808650/1‑148 (MQ=255)
                                                                                                                             ttCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGtctc                  <  2:259688/148‑1 (MQ=255)
                                                                                                                              tCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                 <  1:2983813/148‑1 (MQ=255)
                                                                                                                              tCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                 <  1:446633/148‑1 (MQ=255)
                                                                                                                              tCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                 >  1:937189/1‑148 (MQ=255)
                                                                                                                              tCGATTCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCa                 >  2:640849/1‑148 (MQ=255)
                                                                                                                                  ttCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAc             >  2:2871456/1‑148 (MQ=255)
                                                                                                                                   tCATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTAcc            <  2:2130503/148‑1 (MQ=255)
                                                                                                                                    cATATCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCg           <  1:3561583/148‑1 (MQ=255)
                                                                                                                                      tatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtg         <  2:1146117/148‑1 (MQ=255)
                                                                                                                                      tatCGCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCgtg         >  2:198010/1‑148 (MQ=255)
                                                                                                                                          gCTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTAtt     <  1:3406528/148‑1 (MQ=255)
                                                                                                                                           cTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTc    <  1:1003763/148‑1 (MQ=255)
                                                                                                                                           cTATGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTc    <  1:1639305/148‑1 (MQ=255)
                                                                                                                                             aTGGAAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTccc  >  1:1648910/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TAGATCAAGGTGCCAGCGCACAGATTCTTTGCCCTGGTCATAAAAATCTTCCAGGCACTGTTCCAGTTCGCGCGCCCGACAGGTTTCTCCGCTTTCAGCAAATAGCTGCCAACGACCGCCAAACATTCGATTCATATCGCTATGGCAATAACGTTTCCCTTGCTTCAGCGCAGGAGGATTCCCGAGGATCACCAGCAACCGCCAACGTAACGGGATCTCGCCGTGAGATATCGCGCCAAGCAACGCGTCCAGCATCTCCACAGGTGCCGTCTCATTACCGTGTATTCCC  >  CP009273/1820743‑1821031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑