Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,825,763 C→A 100% A159A (GCG→GCT)  astC ← succinylornithine transaminase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,825,7630CA100.0% 439.9 / NA 133A159A (GCG→GCT) astCsuccinylornithine transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (74/59);  total (74/59)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 135 total aligned reads displayed.

CTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC  >  CP009273/1825618‑1825907
                                                                                                                                                 |                                                                                                                                                
cTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCCGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGgagc                                                                                                                                                >  1:1195740/1‑148 (MQ=255)
cTTGTAAAAAAGCTTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATGCGCCGGCAGGGgagc                                                                                                                                                >  2:1896679/1‑148 (MQ=255)
  tGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTCTATGCAGCATGACGACTATCCGCCGGCAGTGGAGCaa                                                                                                                                              >  2:3591782/1‑148 (MQ=255)
  tGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCaa                                                                                                                                              >  2:3767857/1‑148 (MQ=255)
    tAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCaaaa                                                                                                                                            >  1:2278305/1‑148 (MQ=255)
      aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATc                                                                                                                                          <  1:2347453/148‑1 (MQ=255)
      aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATc                                                                                                                                          <  2:763898/148‑1 (MQ=255)
      aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATc                                                                                                                                          >  1:1566718/1‑148 (MQ=255)
       aaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATcc                                                                                                                                         <  1:2694823/148‑1 (MQ=255)
       aaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATcc                                                                                                                                         <  1:2694815/148‑1 (MQ=255)
          cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCtgt                                                                                                                                      <  1:2118166/148‑1 (MQ=255)
                 ctggctggCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCTCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAgg                                                                                                                               <  1:2529548/148‑1 (MQ=255)
                  tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggc                                                                                                                              <  1:1743022/148‑1 (MQ=255)
                   ggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCCGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATACTGTGAATAggct                                                                                                                             >  1:1671967/1‑148 (MQ=255)
                    gctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                            >  2:141145/1‑148 (MQ=255)
                    gctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                            >  2:141125/1‑148 (MQ=255)
                     ctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCTCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctgg                                                                                                                           <  1:1417197/148‑1 (MQ=255)
                        gcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGgctggctg                                                                                                                        >  2:342681/1‑148 (MQ=255)
                        gcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGgctggctg                                                                                                                        >  1:400473/1‑148 (MQ=255)
                        gcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGgctggctg                                                                                                                        >  1:3282576/1‑148 (MQ=255)
                         caccacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGc                                                                                                                       >  1:201321/1‑148 (MQ=255)
                         caccacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGc                                                                                                                       >  1:201357/1‑148 (MQ=255)
                              cacCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAcc                                                                                                                  <  2:89997/148‑1 (MQ=255)
                                     ttCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTg                                                                                                           >  1:568056/1‑148 (MQ=255)
                                     ttCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTg                                                                                                           <  2:3787897/148‑1 (MQ=255)
                                      tCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGa                                                                                                          >  1:1631989/1‑148 (MQ=255)
                                       cccccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGAc                                                                                                         <  2:2877359/148‑1 (MQ=255)
                                         cccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAg                                                                                                       <  2:843812/148‑1 (MQ=255)
                                          ccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGt                                                                                                      >  1:88051/1‑148 (MQ=255)
                                          ccTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGt                                                                                                      <  1:1917653/148‑1 (MQ=255)
                                                tGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATATGCTGGCTGCCCACCCGCACTGACAGTAAACAg                                                                                                >  1:3530955/1‑148 (MQ=255)
                                                tGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAg                                                                                                >  1:3012860/1‑148 (MQ=255)
                                                tGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAg                                                                                                >  1:3528595/1‑148 (MQ=255)
                                                  ggTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCg                                                                                              <  2:746327/148‑1 (MQ=255)
                                                          aaTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCa                                                                                      <  2:108157/148‑1 (MQ=255)
                                                           aTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCAt                                                                                     >  1:1551056/1‑148 (MQ=255)
                                                           aTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCAt                                                                                     >  1:1554683/1‑148 (MQ=255)
                                                               ccGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaaa                                                                                 >  2:25347/1‑148 (MQ=255)
                                                               ccGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaaa                                                                                 >  1:3256381/1‑148 (MQ=255)
                                                                   acaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCa                                                                             <  1:739663/148‑1 (MQ=255)
                                                                     aGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtt                                                                           <  2:2725640/148‑1 (MQ=255)
                                                                     aGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtt                                                                           <  2:2725685/148‑1 (MQ=255)
                                                                      ggTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttt                                                                          >  2:235146/1‑148 (MQ=255)
                                                                      ggTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttt                                                                          >  2:1907786/1‑148 (MQ=255)
                                                                       gTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtttt                                                                         >  2:3599583/1‑148 (MQ=255)
                                                                        tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCCCCGGCAGTGGAGCAAAAAACTTTGAATAGGCTGGCTGCCCACCCCCACTGACACTAAACAGCGTGCGACCAAGAAACGCAttttt                                                                        >  1:2398300/1‑148 (MQ=255)
                                                                          gagTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                      <  2:568056/148‑1 (MQ=255)
                                                                          gagTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                      <  1:2145517/148‑1 (MQ=255)
                                                                             tcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACg                                                                   >  1:3732044/1‑148 (MQ=255)
                                                                             tcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACg                                                                   >  1:3282639/1‑148 (MQ=255)
                                                                             tcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACg                                                                   >  1:1841667/1‑148 (MQ=255)
                                                                                tcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCa                                                                <  2:2118404/148‑1 (MQ=255)
                                                                                 cAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCAc                                                               >  2:1489050/1‑148 (MQ=255)
                                                                                   aTCAGCGCGCTGGCAGAGTTAAGATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGa                                                             <  2:710306/148‑1 (MQ=255)
                                                                                    tCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAt                                                            <  2:552774/148‑1 (MQ=255)
                                                                                     cAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATg                                                           <  1:3783883/148‑1 (MQ=255)
                                                                                      aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGc                                                          <  1:2128701/148‑1 (MQ=255)
                                                                                      aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGc                                                          <  2:2441989/148‑1 (MQ=255)
                                                                                      aGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGc                                                          <  1:1436428/148‑1 (MQ=255)
                                                                                       gcgcgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGcc                                                         >  2:588709/1‑148 (MQ=255)
                                                                                          cgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGct                                                      >  2:1390878/1‑148 (MQ=255)
                                                                                          cgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGct                                                      >  2:3286980/1‑148 (MQ=255)
                                                                                           gcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctc                                                     <  1:728223/148‑1 (MQ=255)
                                                                                             tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCtt                                                   <  2:2326316/148‑1 (MQ=255)
                                                                                             tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCtt                                                   <  2:1620618/148‑1 (MQ=255)
                                                                                             tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCtt                                                   <  2:1620668/148‑1 (MQ=255)
                                                                                               gCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                 >  2:2191651/1‑148 (MQ=255)
                                                                                               gCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                 <  2:2191947/148‑1 (MQ=255)
                                                                                               gCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                 >  1:3202907/1‑148 (MQ=255)
                                                                                                cAGAGTTACTCTCGTTATATGCAGCCTGACGAATATCCGCCGGCAGTGGAGCAAACTCCTGTGAATAGGCTGGCTGCCCCCCCGCACTGACAGTAAACAGCGAGCGACCATGAAACGCATTTTTGACCGCCACGATGCCGCTCTTATg                                                <  2:2627871/148‑1 (MQ=255)
                                                                                                cAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATg                                                >  1:1900984/1‑148 (MQ=255)
                                                                                                     ttAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGc                                           <  2:3734954/148‑1 (MQ=255)
                                                                                                         tatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                       >  2:151657/1‑148 (MQ=255)
                                                                                                              ttATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGt                                  >  1:404948/1‑148 (MQ=255)
                                                                                                              ttATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGt                                  >  2:3259483/1‑148 (MQ=255)
                                                                                                               tataTGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTc                                 <  1:933242/148‑1 (MQ=255)
                                                                                                                 tatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGt                               >  2:2744244/1‑148 (MQ=255)
                                                                                                                    gcagcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAg                            >  2:2957150/1‑148 (MQ=255)
                                                                                                                    gcagcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAg                            >  2:2957231/1‑148 (MQ=255)
                                                                                                                       gcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGa                         <  2:1071863/148‑1 (MQ=255)
                                                                                                                       gcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGa                         <  2:287813/148‑1 (MQ=255)
                                                                                                                        caTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGaa                        <  1:2185784/148‑1 (MQ=255)
                                                                                                                        caTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGaa                        <  1:2185524/148‑1 (MQ=255)
                                                                                                                         aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAt                       <  2:1631989/148‑1 (MQ=255)
                                                                                                                         aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAt                       <  2:3310770/148‑1 (MQ=255)
                                                                                                                          tGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                      >  1:2711670/1‑148 (MQ=255)
                                                                                                                            aCGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTa                    >  1:2893549/1‑148 (MQ=255)
                                                                                                                               aaTATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgc                 >  1:1478211/1‑148 (MQ=255)
                                                                                                                               aaTATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgc                 >  1:805007/1‑148 (MQ=255)
                                                                                                                                     cgccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGt           <  1:3005626/148‑1 (MQ=255)
                                                                                                                                      gccgGCAGTGGAGCAAAATCCTGTGAAGAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGtt          <  1:3754632/148‑1 (MQ=255)
                                                                                                                                       ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt         <  1:1082913/148‑1 (MQ=255)
                                                                                                                                       ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt         <  2:2278305/148‑1 (MQ=255)
                                                                                                                                       ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt         >  2:1819833/1‑148 (MQ=255)
                                                                                                                                       ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt         <  1:1082671/148‑1 (MQ=255)
                                                                                                                                       ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGttt         <  1:2989578/148‑1 (MQ=255)
                                                                                                                                        cgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGtttt        <  2:2994047/148‑1 (MQ=255)
                                                                                                                                         gtcAGTGGAGCAATATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGTAACGCATTTTTGAACGCCACGATGCCGCTATTATGGGTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTa       <  1:3063463/146‑1 (MQ=255)
                                                                                                                                              tGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGcgcc  >  1:978368/1‑148 (MQ=255)
                                                                                                                                              tGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGcgcc  >  1:611947/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCC  >  CP009273/1825618‑1825907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑