Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,836,192 T→A 100% I67I (ATA→ATT)  ynjH ← DUF1496 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,836,1920TA98.3% 549.1 / 0.1 172I67I (ATA→ATT) ynjHDUF1496 family protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (79/90);  total (81/91)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 172 total aligned reads displayed.

CTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGG  >  CP009273/1836047‑1836335
                                                                                                                                                 |                                                                                                                                               
ctggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTTTCATCACGCTGGCATTGCAGCAGAAt                                                                                                                                               >  2:2444764/1‑148 (MQ=255)
ctggtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAAt                                                                                                                                               <  2:1421055/148‑1 (MQ=255)
   gtctggtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTcc                                                                                                                                            <  2:3667419/148‑1 (MQ=255)
        gtGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCCCCACACCACCGGGTTAGTGCTAAGGGTTTTTTAATAAAGCTGGCATTTCAGCAGAAATccatagg                                                                                                                                       >  1:1943291/1‑143 (MQ=255)
          gCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCt                                                                                                                                     <  2:3432545/148‑1 (MQ=255)
            tCAGCTGATGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATTGATTTACGCGCCGCCAGACCTTCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGTCATTGCAGCAGAATTCCTTCGTCttt                                                                                                                                   <  2:1014267/148‑1 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTATTGCTAATGGTTTTTTCATCACCCTGGCATTGCAGCAGAAATCCTTCGGCTTTaa                                                                                                                                 >  1:2856665/1‑148 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 >  2:2169357/1‑148 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 <  2:3746459/148‑1 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 <  1:265608/148‑1 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 <  1:2425196/148‑1 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 <  1:2194414/148‑1 (MQ=255)
              aCCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATAACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTaa                                                                                                                                 >  1:2131439/1‑148 (MQ=255)
               ccTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAt                                                                                                                                >  2:3022392/1‑148 (MQ=255)
                cTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAAtt                                                                                                                               <  2:295554/148‑1 (MQ=255)
                 tGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTa                                                                                                                              <  1:3579264/148‑1 (MQ=255)
                   aaGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCAATGCAGCAGAATTCCTTCGGCTTTAATTAcc                                                                                                                            >  2:2864498/1‑148 (MQ=255)
                    agagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCg                                                                                                                           <  1:3363735/148‑1 (MQ=255)
                     gagGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCACCGGCTTAGTGCTAAGGGTTTTGTCATCACCCTGGCATTGCAGCAGAATTCCGTAGGCGTTAATTaaccc                                                                                                                          >  2:655924/1‑144 (MQ=255)
                        gcgcTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGcccc                                                                                                                       <  2:1537800/148‑1 (MQ=255)
                              cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGa                                                                                                                 <  1:262892/148‑1 (MQ=255)
                              cAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCGTAGGCTTTAATTACCGCCCCTTCAGa                                                                                                                 >  1:3693550/1‑148 (MQ=255)
                                 tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCCGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTAGGCTTTAATTACCGCCCCTTCAGaata                                                                                                              >  2:2408768/1‑148 (MQ=255)
                                 tatCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGaata                                                                                                              <  2:1625444/148‑1 (MQ=255)
                                    ccGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTACTTCGGCTTTAATTAACGCCCCTTCAGAATAAtt                                                                                                           >  2:3627040/1‑148 (MQ=255)
                                       cTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTg                                                                                                        <  2:2275573/148‑1 (MQ=255)
                                        tGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGa                                                                                                       <  1:2043248/148‑1 (MQ=255)
                                         ggCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAt                                                                                                      <  2:3102299/148‑1 (MQ=255)
                                         ggCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAt                                                                                                      <  1:2399143/148‑1 (MQ=255)
                                              ctgctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGa                                                                                                 <  2:862663/148‑1 (MQ=255)
                                                 ctgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGTCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaa                                                                                              <  2:2091254/148‑1 (MQ=255)
                                                  tgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaaa                                                                                             <  1:1263808/148‑1 (MQ=255)
                                                  tgACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCACCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATaaa                                                                                             <  1:2008488/148‑1 (MQ=255)
                                                    aCATTCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTTGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATACTGATAAACg                                                                                           >  2:1887197/1‑148 (MQ=255)
                                                      aTTCCATTATTAGAGTCGTTTATGGCTTTACGCGCCGCCAGACCTGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTTGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACgca                                                                                         <  2:626671/148‑1 (MQ=255)
                                                        tCCATTATTAGAGGCGTTTATGGCTTTAAGCGCCGCCAGACCACCGGATTAGTGCTAAGGGTTTTGTCAAAACGCTGGCATTGCAGCAGAATTCCATCGGCTTTAATTACCGCCCCATAAAAATAAATTTTATCCTGATAAACccagc                                                                                       >  2:1675506/1‑148 (MQ=255)
                                                          cATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTCGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCAc                                                                                     >  2:1228079/1‑148 (MQ=255)
                                                            ttattaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGTTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATACTTTTGATCCTGATAAACGCAGCACTg                                                                                   >  2:1525081/1‑148 (MQ=255)
                                                            ttattaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTg                                                                                   <  2:2352128/148‑1 (MQ=255)
                                                               ttaGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGt                                                                                <  1:2120701/148‑1 (MQ=255)
                                                                taGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTAACGCCCCTTCAGAATAATTTTGATACTGATAAACGCAGCACTGAGTa                                                                               >  2:3679988/1‑148 (MQ=255)
                                                                        tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                                       >  1:2916592/1‑148 (MQ=255)
                                                                        tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                                       >  1:2636163/1‑148 (MQ=255)
                                                                        tttATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTg                                                                       >  2:1826877/1‑148 (MQ=255)
                                                                              gCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGa                                                                 >  1:388133/1‑148 (MQ=255)
                                                                               cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                                >  1:2682116/1‑148 (MQ=255)
                                                                               cTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGAc                                                                >  1:773257/1‑148 (MQ=255)
                                                                                tttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACt                                                               >  2:3646417/1‑148 (MQ=255)
                                                                                 ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                              >  1:594351/1‑148 (MQ=255)
                                                                                 ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                              >  1:1626293/1‑148 (MQ=255)
                                                                                 ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                              >  2:1219890/1‑148 (MQ=255)
                                                                                 ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                              >  1:1897004/1‑148 (MQ=255)
                                                                                 ttACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTg                                                              >  1:594182/1‑148 (MQ=255)
                                                                                  tACGCGCCGCCAGACCCGCGGATTTGTGCTAAGGGTTTGGTCATCACGCGGGCATTGCAGCAGATTTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGc                                                             <  1:1052217/148‑1 (MQ=255)
                                                                                      cgccgcCAGACCCGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGAGCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGc                                                         <  1:2134619/148‑1 (MQ=255)
                                                                                      cgccgcCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGc                                                         >  1:3190057/1‑148 (MQ=255)
                                                                                           ccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa                                                    <  2:1733618/148‑1 (MQ=255)
                                                                                           ccAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaa                                                    <  2:1733628/148‑1 (MQ=255)
                                                                                            cAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaac                                                   >  2:262421/1‑148 (MQ=255)
                                                                                              gACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTACTTCGGCTTTAATTACCGCCCCTTCAGAATAATATTGATACTGATAAACGCAGCACTGAGTACAGGGCTGCGCACACTGCCCGCcagaactg                                                 >  1:526058/1‑148 (MQ=255)
                                                                                                ccAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactgaa                                               <  1:943254/148‑1 (MQ=255)
                                                                                                ccAGCGGATTAGTGCTAAGGGTTGTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCctgaactgaa                                               <  2:1481974/148‑1 (MQ=255)
                                                                                                 cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                              <  2:2453626/148‑1 (MQ=255)
                                                                                                 cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                              <  2:953108/148‑1 (MQ=255)
                                                                                                 cAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCtgaactgaac                                              <  1:3022392/148‑1 (MQ=255)
                                                                                                  aGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACCGGGCTGCGCTGACTGCCCGCCTGAACTGAaca                                             <  1:1856125/148‑1 (MQ=255)
                                                                                                   gCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAacac                                            <  1:3445412/148‑1 (MQ=255)
                                                                                                    cGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACt                                           >  1:845053/1‑148 (MQ=255)
                                                                                                      gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                         <  1:1254311/148‑1 (MQ=255)
                                                                                                      gATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTc                                         >  2:2321317/1‑148 (MQ=255)
                                                                                                       aTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                        >  2:141479/1‑148 (MQ=255)
                                                                                                       aTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                        >  2:2071968/1‑148 (MQ=255)
                                                                                                       aTTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCt                                        <  2:3036036/148‑1 (MQ=255)
                                                                                                        ttAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCATAATAATTTTGAGCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTg                                       <  1:2322676/148‑1 (MQ=255)
                                                                                                        ttAGTGCTAAGGGTTTTGACATCACGCTGGCATTGCAGCAGAATTCCTTCGGATTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTg                                       <  1:2325354/148‑1 (MQ=255)
                                                                                                         tAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACAGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTgg                                      >  1:2766023/1‑148 (MQ=255)
                                                                                                            tGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAAGTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCgg                                   >  1:3602932/1‑148 (MQ=255)
                                                                                                              cTAAGGGTTTTGTCATCACGCTGGCATTGCAGCATAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCCGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTa                                 <  1:1148184/148‑1 (MQ=255)
                                                                                                              cTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTa                                 <  1:3055128/148‑1 (MQ=255)
                                                                                                              cTAAGGGTTTGGTAATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTa                                 <  1:161812/148‑1 (MQ=255)
                                                                                                               tAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTAc                                >  1:1676256/1‑148 (MQ=255)
                                                                                                                aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                               >  1:2037278/1‑148 (MQ=255)
                                                                                                                aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                               >  2:1171463/1‑148 (MQ=255)
                                                                                                                aaGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAACACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACg                               <  1:1552151/148‑1 (MQ=255)
                                                                                                                 aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                              <  1:3798690/148‑1 (MQ=255)
                                                                                                                 aGGGTTTTGTCATCACGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGt                              >  1:590264/1‑148 (MQ=255)
                                                                                                                           catcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCAc                    >  1:3226040/1‑148 (MQ=255)
                                                                                                                             tcaCGCTGGCATTGCAGCAGAATTCCTTCTGCTTTAATTACCGCCCCTTCAGAATAATTTTCATCCTCATAAACGCCGCACTTTGTACAGGGCTTCGCTGACTGACCGCCTGAACTGAACACTTCTGGCGGTAAGTTTACCTCCACgg                  <  1:467378/148‑2 (MQ=255)
                                                                                                                             tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                  <  2:2543559/148‑1 (MQ=255)
                                                                                                                             tcaCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGt                  >  1:3340429/1‑148 (MQ=255)
                                                                                                                              caCGCTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTc                 <  2:590264/148‑1 (MQ=255)
                                                                                                                                  cTGGCTTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGa             <  1:2070169/148‑1 (MQ=255)
                                                                                                                                  cTGGCATTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGa             <  2:2856665/148‑1 (MQ=255)
                                                                                                                                     gaaTTGCAGCAGAATTCCTTCGGCTTTAATAACCGCCCCTTCAGAATATTTTTGATCCTTATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGa          <  1:45468/146‑1 (MQ=255)
                                                                                                                                       aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATa        <  1:1827513/148‑1 (MQ=255)
                                                                                                                                       aTTGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATa        <  1:1228079/148‑1 (MQ=255)
                                                                                                                                        ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa       >  2:571824/1‑148 (MQ=255)
                                                                                                                                        ttGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATaa       <  2:1385074/148‑1 (MQ=255)
                                                                                                                                         tGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt      >  1:3560419/1‑148 (MQ=255)
                                                                                                                                         tGCAGCAGAATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAAt      >  1:3560711/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTGGTCTGGTGCTCACCTGAAGAGGCGCTGCAATATCCGCTGGCCCCTGCTGACATTCCATTATTAGAGGCGTTTATGGCTTTACGCGCCGCCAGACCAGCGGATTAGTGCTAAGGGTTTTGTCATCACGCTGGCATTGCAGCAGTATTCCTTCGGCTTTAATTACCGCCCCTTCAGAATAATTTTGATCCTGATAAACGCAGCACTGAGTACAGGGCTGCGCTGACTGCCCGCCTGAACTGAACACTTCTGGCGGTACGTTTACCTCCACGTCCGGACGATAATGCGG  >  CP009273/1836047‑1836335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑