Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,860,183 G→T 100% R183S (CGC→AGC)  mipA ← scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,860,1830GT99.5% 652.1 / ‑4.3 206R183S (CGC→AGC) mipAscaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (89/116);  total (90/116)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

TCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGT  >  CP009273/1860039‑1860326
                                                                                                                                                |                                                                                                                                               
tCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGCTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGc                                                                                                                                              >  2:2284013/1‑148 (MQ=255)
   ggCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGa                                                                                                                                           <  2:656207/148‑1 (MQ=255)
      tGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGActc                                                                                                                                        <  1:2063659/148‑1 (MQ=255)
      ggTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGActc                                                                                                                                        <  2:3140640/147‑1 (MQ=255)
       gTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGActct                                                                                                                                       <  1:1761600/148‑1 (MQ=255)
        tCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCtt                                                                                                                                      >  1:2482730/1‑148 (MQ=255)
        tCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCtt                                                                                                                                      <  2:1519509/148‑1 (MQ=255)
          aGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTg                                                                                                                                    <  2:2968344/148‑1 (MQ=255)
            tAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTgcg                                                                                                                                  <  1:3251450/148‑1 (MQ=255)
             aaCTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTgcgc                                                                                                                                 >  1:745820/1‑148 (MQ=255)
                     cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGcc                                                                                                                         >  2:2794511/1‑148 (MQ=255)
                     cagacagaCGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGcc                                                                                                                         >  2:1948146/1‑148 (MQ=255)
                      agacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCa                                                                                                                        <  2:1170832/148‑1 (MQ=255)
                       gacagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCat                                                                                                                       >  2:1815035/1‑148 (MQ=255)
                        acagacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCata                                                                                                                      <  1:1358279/148‑1 (MQ=255)
                          agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataat                                                                                                                    <  2:2481088/148‑1 (MQ=255)
                          agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataat                                                                                                                    >  2:223868/1‑148 (MQ=255)
                          agacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataat                                                                                                                    >  2:836223/1‑148 (MQ=255)
                           gacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataata                                                                                                                   >  2:344418/1‑148 (MQ=255)
                           gacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataata                                                                                                                   >  1:1249428/1‑148 (MQ=255)
                           gacGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCataata                                                                                                                   <  1:1864279/148‑1 (MQ=255)
                            acGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAg                                                                                                                  >  2:798758/1‑148 (MQ=255)
                              gggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTa                                                                                                                <  2:48604/148‑1 (MQ=255)
                               ggTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTAt                                                                                                               >  2:2532112/1‑148 (MQ=255)
                                gtgtAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTAtt                                                                                                              <  2:443094/148‑1 (MQ=255)
                                  gtAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTCGCGGACTCTTTGCGCGATACGCCATAATAGTATTCg                                                                                                            >  2:2113117/1‑148 (MQ=255)
                                     gCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTc                                                                                                         >  2:2228002/1‑148 (MQ=255)
                                       gTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTg                                                                                                       <  1:230959/148‑1 (MQ=255)
                                       gTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTg                                                                                                       <  1:2161282/148‑1 (MQ=255)
                                       gTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTAGAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTg                                                                                                       <  2:2433294/148‑1 (MQ=255)
                                        tGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTgg                                                                                                      >  2:1280417/1‑148 (MQ=255)
                                        tGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTgg                                                                                                      >  2:1207364/1‑148 (MQ=255)
                                         gCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                                                                                     <  1:400878/148‑1 (MQ=255)
                                         gCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGt                                                                                                     <  1:2119292/148‑1 (MQ=255)
                                          cGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGtt                                                                                                    <  1:2227043/148‑1 (MQ=255)
                                             tACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTc                                                                                                 >  1:1912271/1‑148 (MQ=255)
                                                  tAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGt                                                                                            >  1:3089764/1‑148 (MQ=255)
                                                  tAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGt                                                                                            <  1:3487159/148‑1 (MQ=255)
                                                    aaCACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTc                                                                                          >  2:3743041/1‑148 (MQ=255)
                                                    aaCACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTc                                                                                          <  2:63865/148‑1 (MQ=255)
                                                    aaCACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTc                                                                                          >  2:2178202/1‑148 (MQ=255)
                                                      cacTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCa                                                                                        >  2:1850687/1‑148 (MQ=255)
                                                       acTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATATCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCAc                                                                                       >  1:2352027/1‑148 (MQ=255)
                                                         tCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTg                                                                                     >  1:2305334/1‑148 (MQ=255)
                                                             gTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGcaca                                                                                 >  1:192597/1‑148 (MQ=255)
                                                              tCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCacac                                                                                <  2:558619/148‑1 (MQ=255)
                                                                 ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCATACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCaa                                                                             >  1:2513943/1‑148 (MQ=255)
                                                                 ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCaa                                                                             <  1:1731938/148‑1 (MQ=255)
                                                                 ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCaa                                                                             <  2:2359456/148‑1 (MQ=255)
                                                                 ccGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCaa                                                                             <  1:1853786/148‑1 (MQ=255)
                                                                  cGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTGGTTCTGGTTTTAGCTGTTCCACAGCACACCAAt                                                                            >  1:2894469/1‑148 (MQ=255)
                                                                  cGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAAt                                                                            <  1:1116181/148‑1 (MQ=255)
                                                                    aGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAc                                                                          >  2:3807104/1‑148 (MQ=255)
                                                                     ggAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAcc                                                                         <  1:798774/148‑1 (MQ=255)
                                                                      gAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCCTAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATAccc                                                                        >  2:2282096/1‑148 (MQ=255)
                                                                       aaGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATATCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCg                                                                       >  1:3709716/1‑148 (MQ=255)
                                                                       aaGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTCGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCg                                                                       >  1:3080306/1‑148 (MQ=255)
                                                                         gTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGa                                                                     <  2:3633320/148‑1 (MQ=255)
                                                                         gTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGa                                                                     <  1:946269/148‑1 (MQ=255)
                                                                          ttGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAg                                                                    >  2:2308174/1‑148 (MQ=255)
                                                                             tAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCa                                                                 <  2:2862349/148‑1 (MQ=255)
                                                                               gCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACg                                                               >  1:2864676/1‑148 (MQ=255)
                                                                                   gcgcTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCa                                                           <  2:1437330/148‑1 (MQ=255)
                                                                                        cAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCa                                                      <  2:3344404/148‑1 (MQ=255)
                                                                                         aGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCAc                                                     <  1:3151822/148‑1 (MQ=255)
                                                                                                 gTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgtg                                             >  1:2538039/1‑148 (MQ=255)
                                                                                                  tAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGgtgt                                            >  2:191257/1‑148 (MQ=255)
                                                                                                    aGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTaa                                          >  2:739334/1‑148 (MQ=255)
                                                                                                    aGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTaa                                          <  1:2284013/148‑1 (MQ=255)
                                                                                                    aGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTaa                                          <  2:529053/148‑1 (MQ=255)
                                                                                                    aGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCAGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCCGAGTCACGGTCAGGCCACCCTTGGTGTaa                                          >  1:1356111/1‑148 (MQ=255)
                                                                                                     gggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAAc                                         >  2:3017416/1‑148 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTTCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        >  1:3592406/1‑148 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        >  2:3179911/1‑148 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        <  2:1536153/148‑1 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        <  1:830662/148‑1 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        >  2:465397/1‑148 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        <  1:2635529/148‑1 (MQ=255)
                                                                                                      ggCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACg                                        >  1:3551090/1‑148 (MQ=255)
                                                                                                        cTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGAt                                      >  1:3154120/1‑148 (MQ=255)
                                                                                                        cTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGAt                                      <  1:465397/148‑1 (MQ=255)
                                                                                                             gCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAAc                                 >  2:595536/1‑148 (MQ=255)
                                                                                                               tGTCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCa                               >  2:3468559/1‑148 (MQ=255)
                                                                                                                 tCGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAgg                             <  1:2178202/148‑1 (MQ=255)
                                                                                                                  cGTTCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGc                            <  1:2257121/148‑1 (MQ=255)
                                                                                                                     tCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCat                         >  1:2374535/1‑148 (MQ=255)
                                                                                                                     tCGGGTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCat                         <  1:3206619/148‑1 (MQ=255)
                                                                                                                       gggTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCatat                       <  2:3089764/148‑1 (MQ=255)
                                                                                                                         gTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATcc                     <  2:3197062/148‑1 (MQ=255)
                                                                                                                         gTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATcc                     <  1:2961831/148‑1 (MQ=255)
                                                                                                                         gTTATAGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATcc                     <  1:1948146/148‑1 (MQ=255)
                                                                                                                            ataGCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAg                  <  1:2520952/148‑1 (MQ=255)
                                                                                                                               gCCACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACg               <  2:2015030/148‑1 (MQ=255)
                                                                                                                                ccACGCAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGa              <  2:174971/148‑1 (MQ=255)
                                                                                                                                     cAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCg         <  1:1477691/148‑1 (MQ=255)
                                                                                                                                     cAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCg         <  2:2873480/148‑1 (MQ=255)
                                                                                                                                     cAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCg         <  2:2753051/148‑1 (MQ=255)
                                                                                                                                     cAGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGGTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCg         <  2:2755156/148‑1 (MQ=255)
                                                                                                                                      aGACCGCTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGt        <  1:795589/148‑1 (MQ=255)
                                                                                                                                            cTGCTAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGt  >  1:699954/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCCATAATAGTATTCGTTCTGGTTTTCGCTGTTCCACTGCACACCAATACCCGGAGTCACGGTCAGGCCACCGTTGGTGTAACGATACAACCAGGCCATATCCCAGACGATGCCGTTGCTGT  >  CP009273/1860039‑1860326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑