Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,863,753 C→A 7.8% T181K (ACG→AAG)  yeaH → UPF0229 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,863,7530CA 7.8% 888.4 / 30.9 242T181K (ACG→AAG) yeaHUPF0229 family protein
Reads supporting (aligned to +/- strand):  ref base C (108/115);  new base A (10/9);  total (118/124)
Fisher's exact test for biased strand distribution p-value = 8.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 242 total aligned reads displayed.

TACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTG  >  CP009273/1863612‑1863899
                                                                                                                                             |                                                                                                                                                  
tACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACggcagg                                                                                                                                              >  1:1602400/1‑148 (MQ=255)
         ttaaacaaaacaAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAgcggcg                                                                                                                                     <  1:1694929/146‑1 (MQ=255)
         tGAAAAAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCTTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAgcggcg                                                                                                                                     <  1:2916630/148‑1 (MQ=255)
          gAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                                                                                                    <  2:3627314/148‑1 (MQ=255)
          gAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                                                                                                    >  2:2116065/1‑148 (MQ=255)
          gAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCATTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGcggcgg                                                                                                                                    >  2:2115871/1‑148 (MQ=255)
           aaaCAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCggg                                                                                                                                   >  1:1971336/1‑148 (MQ=255)
            aaCAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGa                                                                                                                                  <  1:2724896/148‑1 (MQ=255)
             aCAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGaa                                                                                                                                 <  2:2834230/148‑1 (MQ=255)
             aCAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGaa                                                                                                                                 >  2:1999408/1‑148 (MQ=255)
              cAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAAc                                                                                                                                >  1:3056364/1‑148 (MQ=255)
              cAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAAc                                                                                                                                <  2:1549812/148‑1 (MQ=255)
              cAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAAc                                                                                                                                >  1:356573/1‑148 (MQ=255)
              cAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAAc                                                                                                                                <  2:3437307/148‑1 (MQ=255)
                 aaCCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTc                                                                                                                             <  1:2621543/148‑1 (MQ=255)
                   ccAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCAt                                                                                                                           <  2:470115/148‑1 (MQ=255)
                    caacaaCGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATg                                                                                                                          >  2:244418/1‑148 (MQ=255)
                      acaacGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCa                                                                                                                        >  2:1462164/1‑148 (MQ=255)
                       caacGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCAc                                                                                                                       >  2:2364410/1‑148 (MQ=255)
                       caacGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCAc                                                                                                                       >  2:382889/1‑148 (MQ=255)
                       caacGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCAc                                                                                                                       >  2:1193245/1‑148 (MQ=255)
                       caacGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCAc                                                                                                                       <  2:2915797/148‑1 (MQ=255)
                        aacGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACt                                                                                                                      <  1:2011495/148‑1 (MQ=255)
                         acGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTg                                                                                                                     >  1:3779690/1‑148 (MQ=255)
                         acGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTg                                                                                                                     >  1:2181870/1‑148 (MQ=255)
                          cGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTgg                                                                                                                    <  1:721481/148‑1 (MQ=255)
                           gCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGa                                                                                                                   <  1:3256713/148‑1 (MQ=255)
                                cTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAgaga                                                                                                              >  1:252854/1‑148 (MQ=255)
                                cTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAACCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAgaga                                                                                                              >  1:78877/1‑148 (MQ=255)
                                 tGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGaa                                                                                                             <  2:1374341/148‑1 (MQ=255)
                                   aCCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTGATGCACTGGAAGAGAAtt                                                                                                           >  2:103731/1‑148 (MQ=255)
                                    ccGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAAttt                                                                                                          <  2:933332/148‑1 (MQ=255)
                                         tataAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCca                                                                                                     >  1:3132093/1‑148 (MQ=255)
                                              aaCGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCatcatc                                                                                                <  2:3294244/148‑1 (MQ=255)
                                               aCGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCAtcatca                                                                                               <  1:2197254/148‑1 (MQ=255)
                                               aCGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCAtcatca                                                                                               >  1:892439/1‑148 (MQ=255)
                                                cGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAg                                                                                              >  2:771575/1‑148 (MQ=255)
                                                  cATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCa                                                                                            <  1:2862346/148‑1 (MQ=255)
                                                  cATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCa                                                                                            <  2:3387881/148‑1 (MQ=255)
                                                  cATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCa                                                                                            <  2:3388089/148‑1 (MQ=255)
                                                      gggcgggTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAg                                                                                        >  1:3299775/1‑148 (MQ=255)
                                                         cgggTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGa                                                                                     <  1:2399841/148‑1 (MQ=255)
                                                          gggTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGaa                                                                                    <  1:1093149/148‑1 (MQ=255)
                                                          gggTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGaa                                                                                    <  1:1379394/148‑1 (MQ=255)
                                                           ggTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAAc                                                                                   >  1:365063/1‑148 (MQ=255)
                                                             ttATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCt                                                                                 >  2:2557157/1‑148 (MQ=255)
                                                              tataCCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTg                                                                                <  2:3554107/148‑1 (MQ=255)
                                                               ataCCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTgc                                                                               >  2:1109439/1‑148 (MQ=255)
                                                                 aCCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTgcgc                                                                             >  1:1853400/1‑148 (MQ=255)
                                                                   cGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCaa                                                                           <  2:2935992/148‑1 (MQ=255)
                                                                    gCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAAc                                                                          <  2:314390/148‑1 (MQ=255)
                                                                      tAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActg                                                                        <  2:34771/148‑1 (MQ=255)
                                                                       aaCGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgc                                                                       <  1:3572907/148‑1 (MQ=255)
                                                                        aCGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAActgct                                                                      <  1:3737532/148‑1 (MQ=255)
                                                                           gCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGa                                                                   >  1:1328626/1‑148 (MQ=255)
                                                                           gCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGa                                                                   <  1:3716810/148‑1 (MQ=255)
                                                                            cGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGaa                                                                  <  2:1917489/148‑1 (MQ=255)
                                                                             gTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAag                                                                 <  2:1658652/148‑1 (MQ=255)
                                                                              ttCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAaga                                                                >  2:672595/1‑148 (MQ=255)
                                                                               tCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAagag                                                               >  2:3396390/1‑148 (MQ=255)
                                                                                ccGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAgg                                                              <  2:361785/148‑1 (MQ=255)
                                                                                ccGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAgg                                                              <  2:2046862/148‑1 (MQ=255)
                                                                                 cGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGa                                                             <  1:2158426/148‑1 (MQ=255)
                                                                                    ccAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACg                                                          >  1:648329/1‑148 (MQ=255)
                                                                                     cAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGt                                                         <  2:3199672/148‑1 (MQ=255)
                                                                                     cAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGt                                                         <  2:2156548/148‑1 (MQ=255)
                                                                                       atatCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCt                                                       >  1:97501/1‑148 (MQ=255)
                                                                                       atatCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCt                                                       >  1:191215/1‑148 (MQ=255)
                                                                                         atCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGACCGTCTgc                                                     >  2:1094323/1‑148 (MQ=255)
                                                                                          tCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTgcg                                                    <  1:1156619/148‑1 (MQ=255)
                                                                                          tCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTgcg                                                    >  1:3476061/1‑148 (MQ=255)
                                                                                            aGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCa                                                  >  1:1367758/1‑148 (MQ=255)
                                                                                              cGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCaaa                                                >  1:2952862/1‑148 (MQ=255)
                                                                                              cGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCaaa                                                >  1:546849/1‑148 (MQ=255)
                                                                                              cGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCaaa                                                <  2:3307404/148‑1 (MQ=255)
                                                                                                ttGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa                                              >  1:193083/1‑148 (MQ=255)
                                                                                                     cGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTg                                         >  1:1509062/1‑148 (MQ=255)
                                                                                                        tCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAg                                      >  1:267532/1‑148 (MQ=255)
                                                                                                           ttGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAAt                                   >  1:1263462/1‑148 (MQ=255)
                                                                                                            tGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAAtt                                  <  1:3155087/148‑1 (MQ=255)
                                                                                                            tGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAAtt                                  >  1:1209589/1‑148 (MQ=255)
                                                                                                              cAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTAc                                >  2:82582/1‑148 (MQ=255)
                                                                                                               aGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACg                               <  2:356573/148‑1 (MQ=255)
                                                                                                                  aCTCACTGGCGCGCCGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGc                            <  1:3678926/148‑1 (MQ=255)
                                                                                                                  aCTCACTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGc                            <  1:86546/148‑1 (MQ=255)
                                                                                                                       cTGGCGCGACGCACAGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCaaaa                       <  1:385534/148‑1 (MQ=255)
                                                                                                                           cgcgACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGa                   <  2:3807750/148‑1 (MQ=255)
                                                                                                                                   acaGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTcc           <  2:546849/148‑1 (MQ=255)
                                                                                                                                   acaGCCATGAAGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTcc           <  2:3544204/148‑1 (MQ=255)
                                                                                                                                    caGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTccc          <  1:1073745/148‑1 (MQ=255)
                                                                                                                                      gCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCtt        <  2:961910/148‑1 (MQ=255)
                                                                                                                                      gCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCtt        <  1:610681/148‑1 (MQ=255)
                                                                                                                                       ccATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCttt       >  2:1754807/1‑148 (MQ=255)
                                                                                                                                       ccATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCttt       >  1:854756/1‑148 (MQ=255)
                                                                                                                                         aTGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTa     >  2:2275227/1‑148 (MQ=255)
                                                                                                                                          tGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAt    <  1:3564233/148‑1 (MQ=255)
                                                                                                                                          tGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAt    <  1:2060136/148‑1 (MQ=255)
                                                                                                                                           gACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt   >  2:1567080/1‑148 (MQ=255)
                                                                                                                                            aCGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTg  <  1:2115875/148‑1 (MQ=255)
                                                                                                                                            aCGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTg  <  1:2115775/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                  
TACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCGAATATAAAACGCATCGGGCGGGTTATACCGCTAACGGCGTTCCGGCCAATATCAGCGTTGTGCGTTCATTGCAGAACTCACTGGCGCGACGCACAGCCATGACGGCAGGCAAGCGGCGGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATTG  >  CP009273/1863612‑1863899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑