BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,872,520	G→T	100%	P183P (CCG→CCT)	yeaP →	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,872,520	0	G	T	100.0%	908.4 / NA	270	P183P (CCG→CCT)	yeaP	diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (125/145); total (125/145)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 270 total aligned reads displayed.

GGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTT  >  CP009273/1872375‑1872664

ggAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTaa                                                                                                                                                <  2:202227/148‑1 (MQ=255)

           gcgGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGa                                                                                                                                     <  1:2783107/148‑1 (MQ=255)

            cgGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAt                                                                                                                                    <  1:2505375/148‑1 (MQ=255)

            cgGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAt                                                                                                                                    <  1:633589/148‑1 (MQ=255)

              gAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAttt                                                                                                                                  <  2:1771273/148‑1 (MQ=255)

               aaCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAtttt                                                                                                                                 >  1:3260913/1‑148 (MQ=255)

                aCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAttttt                                                                                                                                >  2:2589377/1‑148 (MQ=255)

                aCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAttttt                                                                                                                                >  1:3667767/1‑148 (MQ=255)

                aCAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGAttttt                                                                                                                                >  1:2141641/1‑148 (MQ=255)

                 cAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTg                                                                                                                               >  2:3651644/1‑148 (MQ=255)

                      tttACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAAt                                                                                                                          <  2:2510418/148‑1 (MQ=255)

                          cAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTga                                                                                                                      >  2:1575536/1‑148 (MQ=255)

                          cAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGGTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTGTTGAAAATCTga                                                                                                                      >  2:2441245/1‑148 (MQ=255)

                                ttCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGacac                                                                                                                >  1:2000440/1‑148 (MQ=255)

                                ttCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGacac                                                                                                                >  1:939482/1‑148 (MQ=255)

                                   gCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGt                                                                                                             <  1:568387/148‑1 (MQ=255)

                                      ggACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGtttt                                                                                                          <  1:435302/148‑1 (MQ=255)

                                      ggACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGtttt                                                                                                          <  1:3162424/148‑1 (MQ=255)

                                      ggACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGtttt                                                                                                          <  2:1055632/148‑1 (MQ=255)

                                        aCTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGTAAATCTGACGACACTGTTTTcc                                                                                                        >  1:1635460/1‑148 (MQ=255)

                                        aCTGATTGCACAATATATCCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTcc                                                                                                        <  1:1298727/148‑1 (MQ=255)

                                         cTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTccc                                                                                                       <  1:1578132/148‑1 (MQ=255)

                                         cTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTccc                                                                                                       >  2:2830563/1‑148 (MQ=255)

                                            aTTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCg                                                                                                    >  1:1089096/1‑148 (MQ=255)

                                            aTTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCg                                                                                                    <  1:3104361/148‑1 (MQ=255)

                                            aTTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCg                                                                                                    >  2:3814056/1‑148 (MQ=255)

                                             ttGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCGTCGc                                                                                                   <  1:3640396/148‑1 (MQ=255)

                                                cacaATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCg                                                                                                >  2:1912275/1‑148 (MQ=255)

                                                 acaATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCgg                                                                                               <  1:3572412/148‑1 (MQ=255)

                                                  caATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGc                                                                                              <  2:3404004/148‑1 (MQ=255)

                                                  caATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGc                                                                                              >  1:2599176/1‑148 (MQ=255)

                                                      atatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCt                                                                                          >  1:3125143/1‑148 (MQ=255)

                                                      atatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCt                                                                                          >  2:2552243/1‑148 (MQ=255)

                                                      atatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCt                                                                                          >  2:1151096/1‑148 (MQ=255)

                                                       tatTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCtt                                                                                         >  1:3068381/1‑148 (MQ=255)

                                                        atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTa                                                                                        >  2:1994979/1‑148 (MQ=255)

                                                        atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTa                                                                                        >  2:63282/1‑148 (MQ=255)

                                                        atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTa                                                                                        >  2:1514770/1‑148 (MQ=255)

                                                        atTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTa                                                                                        >  1:1218330/1‑148 (MQ=255)

                                                         ttCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTaa                                                                                       <  2:3398854/148‑1 (MQ=255)

                                                              aaaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATa                                                                                  <  1:771125/148‑1 (MQ=255)

                                                               aaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATaa                                                                                 >  2:683962/1‑148 (MQ=255)

                                                               aaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATaa                                                                                 >  2:605369/1‑148 (MQ=255)

                                                               aaGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATaa                                                                                 >  1:1001846/1‑148 (MQ=255)

                                                                agagGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAg                                                                                >  1:2713868/1‑148 (MQ=255)

                                                                agagGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAg                                                                                >  2:3536720/1‑148 (MQ=255)

                                                                agagGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCACGCCCGGCATCTTAAACATAAg                                                                                >  1:868182/1‑148 (MQ=255)

                                                                  agGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGAt                                                                              <  2:3682277/148‑1 (MQ=255)

                                                                    gCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATaa                                                                            >  1:3324326/1‑148 (MQ=255)

                                                                     cACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAAt                                                                           >  2:1444419/1‑148 (MQ=255)

                                                                       cTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGa                                                                         >  2:1711422/1‑148 (MQ=255)

                                                                          gTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATcg                                                                      <  2:3777356/148‑1 (MQ=255)

                                                                           ttGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATcgc                                                                     >  1:1510335/1‑148 (MQ=255)

                                                                           ttGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATcgc                                                                     >  2:1363600/1‑148 (MQ=255)

                                                                           ttGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATcgc                                                                     >  2:3267329/1‑148 (MQ=255)

                                                                              aaCAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGtt                                                                  >  2:1877098/1‑148 (MQ=255)

                                                                              aaCAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGtt                                                                  <  1:1352376/148‑1 (MQ=255)

                                                                              aaCAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGtt                                                                  >  2:794205/1‑148 (MQ=255)

                                                                                 aGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTAt                                                               <  2:2382113/148‑1 (MQ=255)

                                                                                   cTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCg                                                             >  2:3383304/1‑148 (MQ=255)

                                                                                     gcgcGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGAt                                                           <  1:324152/148‑1 (MQ=255)

                                                                                      cgcgAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATc                                                          <  2:3158325/148‑1 (MQ=255)

                                                                                         gAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTgg                                                       <  2:1819821/148‑1 (MQ=255)

                                                                                          aaGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGa                                                      >  1:573557/1‑148 (MQ=255)

                                                                                           aGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGAt                                                     >  2:3574423/1‑148 (MQ=255)

                                                                                           aGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGAt                                                     <  1:2460769/148‑1 (MQ=255)

                                                                                            gCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATa                                                    >  2:1641572/1‑148 (MQ=255)

                                                                                            gCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATa                                                    <  1:511856/148‑1 (MQ=255)

                                                                                            gCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATa                                                    >  2:521170/1‑148 (MQ=255)

                                                                                                  gctgcGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCa                                              >  1:2981743/1‑148 (MQ=255)

                                                                                                    tgcGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCaaa                                            <  2:2152905/148‑1 (MQ=255)

                                                                                                    tgcGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCaaa                                            <  2:2395000/148‑1 (MQ=255)

                                                                                                     gcgcTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAt                                           >  2:3062864/1‑148 (MQ=255)

                                                                                                      cgcTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAAtt                                          >  2:934170/1‑148 (MQ=255)

                                                                                                        cTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCGACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTaa                                        <  2:2823337/148‑1 (MQ=255)

                                                                                                           aTTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCa                                     <  2:2285802/148‑1 (MQ=255)

                                                                                                           aTTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCa                                     <  1:562538/148‑1 (MQ=255)

                                                                                                             tGCGCAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAAt                                   >  2:1022512/1‑148 (MQ=255)

                                                                                                               cgcAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGa                                 <  1:3131264/148‑1 (MQ=255)

                                                                                                                gcAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGAt                                <  1:3815733/148‑1 (MQ=255)

                                                                                                                 cAATCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATc                               <  2:1695375/148‑1 (MQ=255)

                                                                                                                   aTCGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGt                             >  1:3015264/1‑148 (MQ=255)

                                                                                                                     cGTATACCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGttt                           <  1:2105111/148‑1 (MQ=255)

                                                                                                                        ataCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTgg                        <  2:2686870/148‑1 (MQ=255)

                                                                                                                         taCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGt                       >  1:1975415/1‑148 (MQ=255)

                                                                                                                         taCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGt                       <  1:3651644/148‑1 (MQ=255)

                                                                                                                         taCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGt                       <  1:2418975/148‑1 (MQ=255)

                                                                                                                          aCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTc                      >  2:3510752/1‑148 (MQ=255)

                                                                                                                          aCCGACTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTc                      >  2:748699/1‑148 (MQ=255)

                                                                                                                              aCTCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCataa                  <  1:2912980/148‑1 (MQ=255)

                                                                                                                                tCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCataata                <  2:1134137/148‑1 (MQ=255)

                                                                                                                                tCGTTAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCataata                <  2:210918/148‑1 (MQ=255)

                                                                                                                                    tAACCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTgg            <  2:569354/148‑1 (MQ=255)

                                                                                                                                      aCCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCg          <  1:683962/148‑1 (MQ=255)

                                                                                                                                      aCCGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCg          >  1:1677408/1‑148 (MQ=255)

                                                                                                                                        cGGGCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGAt        <  1:344891/148‑1 (MQ=255)

                                                                                                                                           gCTACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTg     <  1:1514850/148‑1 (MQ=255)

                                                                                                                                             tACCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGtt   <  2:2141641/148‑1 (MQ=255)

                                                                                                                                              aCCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGttt  <  1:2552243/148‑1 (MQ=255)

                                                                                                                                              aCCTAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAAACATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGttt  >  1:2184690/1‑148 (MQ=255)

GGAGTGAACGCGCGGAACAGGTTTTACAGTTATTCGCCGGACTGATTGCACAATATATTCAAAAAGAGGCACTGGTTGAACAGCTGCGCGAAGCCAATGCTGCGCTGATTGCGCAATCGTATACCGACTCGTTAACCGGGCTACCGAATCGGCGGGCGATTTTTGAAAATCTGACGACACTGTTTTCCCTCGCCCGGCATCTTAACCATAAGATAATGATCGCGTTTATCGATCTGGATAACTTCAAATTAATCAATGATCGTTTTGGTCATAATAGTGGCGATCTGTTT  >  CP009273/1872375‑1872664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`