Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,874,924 T→A 100% V31V (GTA→GTT)  leuE ← leucine efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,874,9240TA98.7% 778.3 / ‑0.4 235V31V (GTA→GTT) leuEleucine efflux protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (107/125);  total (109/126)
Fisher's exact test for biased strand distribution p-value = 5.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

TACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCT  >  CP009273/1874780‑1875068
                                                                                                                                                |                                                                                                                                                
tACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACa                                                                                                                                               >  2:1533759/1‑148 (MQ=255)
 aCAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATTACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaa                                                                                                                                              <  2:2257686/148‑1 (MQ=255)
 aCAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACaa                                                                                                                                              <  1:290422/148‑1 (MQ=255)
   aaTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAAc                                                                                                                                            <  1:2467139/148‑1 (MQ=255)
    aTGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGaacaaaca                                                                                                                                           >  2:880091/1‑148 (MQ=255)
        tGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGt                                                                                                                                       >  1:176745/1‑148 (MQ=255)
        tGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGt                                                                                                                                       <  1:3711507/148‑1 (MQ=255)
        tGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGt                                                                                                                                       <  1:2357351/148‑1 (MQ=255)
         gAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTa                                                                                                                                      <  2:3042608/148‑1 (MQ=255)
            taataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTAttt                                                                                                                                   <  2:1636813/148‑1 (MQ=255)
            taataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTAttt                                                                                                                                   >  1:3108619/1‑148 (MQ=255)
            taataTCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTAttt                                                                                                                                   >  2:521613/1‑148 (MQ=255)
               tatCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGc                                                                                                                                >  1:1822261/1‑148 (MQ=255)
                atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGcc                                                                                                                               <  2:1880225/148‑1 (MQ=255)
                atCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGcc                                                                                                                               <  2:317306/148‑1 (MQ=255)
                 tCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                              <  2:1730861/148‑1 (MQ=255)
                 tCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                              <  2:1118276/148‑1 (MQ=255)
                 tCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGccc                                                                                                                              <  1:514530/148‑1 (MQ=255)
                   ggggTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTg                                                                                                                            <  2:2869052/148‑1 (MQ=255)
                    gggTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTgg                                                                                                                           >  1:1218896/1‑148 (MQ=255)
                     ggtggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGc                                                                                                                          >  2:2295758/1‑148 (MQ=255)
                     ggtggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGc                                                                                                                          >  2:372143/1‑148 (MQ=255)
                     ggtggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGc                                                                                                                          >  2:3110483/1‑148 (MQ=255)
                       tggtCTTAATTAATGTCGCCACTCCAGCCCATCCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCAc                                                                                                                        <  2:3343982/148‑1 (MQ=255)
                        ggtCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCAcc                                                                                                                       >  2:44494/1‑148 (MQ=255)
                          tCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCaa                                                                                                                     >  1:3222910/1‑148 (MQ=255)
                              aattaatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACAGCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAAcaca                                                                                                                 <  1:3552620/148‑1 (MQ=255)
                               attaatGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACaa                                                                                                                >  2:1170912/1‑148 (MQ=255)
                                   atGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaa                                                                                                            >  1:3792836/1‑148 (MQ=255)
                                     gTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATaaaaa                                                                                                          >  2:2103733/1‑148 (MQ=255)
                                      tCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAAt                                                                                                         <  2:394912/148‑1 (MQ=255)
                                      tCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAAt                                                                                                         >  1:1712866/1‑148 (MQ=255)
                                             tCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCa                                                                                                  <  2:2199830/148‑1 (MQ=255)
                                              ccAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCaa                                                                                                 >  2:3396311/1‑148 (MQ=255)
                                                 gCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCa                                                                                              >  2:1993036/1‑148 (MQ=255)
                                                  cccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                             <  1:3654647/148‑1 (MQ=255)
                                                  cccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAg                                                                                             >  1:750120/1‑148 (MQ=255)
                                                   ccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGa                                                                                            <  1:1682541/148‑1 (MQ=255)
                                                   ccATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGa                                                                                            >  2:2960648/1‑148 (MQ=255)
                                                      tGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAg                                                                                         <  1:2798595/148‑1 (MQ=255)
                                                         cAGAAACATCAATACCGCATCGCCAATAAAGACACCGCAGGCCGCAAGATAACCGCATTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTTGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTc                                                                                      <  2:1639036/148‑1 (MQ=255)
                                                               cATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTaa                                                                                >  2:314890/1‑148 (MQ=255)
                                                                aTCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAAt                                                                               >  2:1422860/1‑148 (MQ=255)
                                                                aTCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAAt                                                                               <  1:837553/148‑1 (MQ=255)
                                                                     tACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGa                                                                          >  2:1411428/1‑148 (MQ=255)
                                                                      aCCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGaa                                                                         >  1:1155416/1‑148 (MQ=255)
                                                                        cGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAAcc                                                                       <  1:1507720/148‑1 (MQ=255)
                                                                            tCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGt                                                                   <  1:552532/148‑1 (MQ=255)
                                                                             cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTa                                                                  >  2:714456/1‑148 (MQ=255)
                                                                             cGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTa                                                                  <  1:499041/148‑1 (MQ=255)
                                                                               ccAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTAtt                                                                >  2:2035180/1‑148 (MQ=255)
                                                                                  ataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAg                                                             >  1:1487353/1‑148 (MQ=255)
                                                                                  ataaataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAg                                                             <  2:586858/148‑1 (MQ=255)
                                                                                     aataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGa                                                          >  2:483554/1‑148 (MQ=255)
                                                                                      ataCACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGaa                                                         >  1:1619148/1‑148 (MQ=255)
                                                                                          acCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAAcaca                                                     <  2:769088/148‑1 (MQ=255)
                                                                                           ccGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACAt                                                    >  1:3416338/1‑148 (MQ=255)
                                                                                           ccGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACAt                                                    <  1:1471774/148‑1 (MQ=255)
                                                                                             gCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTa                                                  <  1:2103733/148‑1 (MQ=255)
                                                                                             gCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTa                                                  <  1:2341760/148‑1 (MQ=255)
                                                                                              cAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTaa                                                 <  2:1787974/148‑1 (MQ=255)
                                                                                               aGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAAc                                                <  2:2155735/148‑1 (MQ=255)
                                                                                                 gCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACct                                              >  2:1597718/1‑148 (MQ=255)
                                                                                                    gCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCtt                                           <  1:369308/148‑1 (MQ=255)
                                                                                                       aGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTaa                                        >  2:383937/1‑148 (MQ=255)
                                                                                                        gATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAt                                       >  2:2506243/1‑148 (MQ=255)
                                                                                                        gATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAt                                       >  2:3640505/1‑148 (MQ=255)
                                                                                                        gATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAt                                       <  1:3481901/148‑1 (MQ=255)
                                                                                                        gATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAt                                       >  2:33848/1‑148 (MQ=255)
                                                                                                         aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                      >  1:3612870/1‑148 (MQ=255)
                                                                                                         aTAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAAtt                                      >  2:2758501/1‑148 (MQ=255)
                                                                                                             ccGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCt                                  <  2:666937/148‑1 (MQ=255)
                                                                                                              cGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                                 <  1:2605549/148‑1 (MQ=255)
                                                                                                              cGCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                                 >  1:3754383/1‑148 (MQ=255)
                                                                                                              cGCCTTTCATACCGCTACTGAAGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCtt                                 <  2:2372558/148‑1 (MQ=255)
                                                                                                               gCCTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCttt                                <  1:2402836/148‑1 (MQ=255)
                                                                                                                 cTTTCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCg                              <  2:32529/148‑1 (MQ=255)
                                                                                                                   ttCATACCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGAt                            >  1:1399311/1‑148 (MQ=255)
                                                                                                                        aCCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgc                       >  2:301889/1‑148 (MQ=255)
                                                                                                                        aCCGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgc                       >  2:1219109/1‑148 (MQ=255)
                                                                                                                          cGCTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATgcgc                     >  1:1590114/1‑148 (MQ=255)
                                                                                                                            cTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa                   <  1:974691/148‑1 (MQ=255)
                                                                                                                            cTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa                   <  1:2819771/148‑1 (MQ=255)
                                                                                                                            cTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa                   <  1:3745190/148‑1 (MQ=255)
                                                                                                                            cTACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGa                   >  2:1734470/1‑148 (MQ=255)
                                                                                                                             tACTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGAt                  <  1:3504895/148‑1 (MQ=255)
                                                                                                                              aCTGACGCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTAAGCGAACACATTAAACTCTTTAATTATATTTCGATCATGAGCGAtt                 >  1:2176094/1‑148 (MQ=255)
                                                                                                                                 gACGCTATTTTTGAGAACAAACCGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTaaa              <  2:1920659/148‑1 (MQ=255)
                                                                                                                                    gCTATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGt           <  2:2139089/148‑1 (MQ=255)
                                                                                                                                      tATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGa         >  2:1102732/1‑148 (MQ=255)
                                                                                                                                      tATTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGa         <  2:176745/148‑1 (MQ=255)
                                                                                                                                       aTTTTTGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGaa        >  2:2590582/1‑148 (MQ=255)
                                                                                                                                        tttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAat       <  2:1200874/148‑1 (MQ=255)
                                                                                                                                        tttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAat       <  1:885698/148‑1 (MQ=255)
                                                                                                                                        tttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAat       <  1:2146581/148‑1 (MQ=255)
                                                                                                                                        tttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAat       <  1:2894853/148‑1 (MQ=255)
                                                                                                                                         ttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAata      <  1:493905/148‑1 (MQ=255)
                                                                                                                                         ttttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAata      <  1:2284060/148‑1 (MQ=255)
                                                                                                                                           ttGAGAACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATg    <  2:346762/148‑1 (MQ=255)
                                                                                                                                             gagaACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCt  <  1:1881279/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TACAATGTTGAATAATATCGGGGTGGTCTTAATTAATGTCGCCACTCCAGCCCATGCCAGAAACATCAATACCGCATCGCCAATAAATACACCGCAGGCCGCAAGATAACCGCCTTTCATACCGCTACTGACGCTATTTTTGAGTACAAACAGGGTATTTGGCCCTGGCACCAACACAATAAAAATGGCCCCAACCAGATAGGTCCAGTAATTCAGAACCCCGTATTCAGCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCT  >  CP009273/1874780‑1875068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑