Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,885,611 G→T 100% R628S (CGT→AGT)  yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,885,6110GT98.4% 588.1 / 0.7 185R628S (CGT→AGT) yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (126/56);  total (129/56)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 185 total aligned reads displayed.

TCGTTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACC  >  CP009273/1885466‑1885753
                                                                                                                                                 |                                                                                                                                              
tcgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTaa                                                                                                                                              <  1:333576/148‑1 (MQ=255)
 cgtTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGACTTCGCAAGGGTCAACGTGCTTAGATCAATAAAAAGCCGAGCATCATACCATACTCCGTAAAAAATTACCTGGAGGATGGTCTCGCAAGGCAACTAAc                                                                                                                                             >  1:2663599/1‑148 (MQ=255)
      cacaGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCAc                                                                                                                                        <  1:392195/148‑1 (MQ=255)
       acaGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACg                                                                                                                                       <  1:1552813/148‑1 (MQ=255)
        caGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACgg                                                                                                                                      >  1:48496/1‑148 (MQ=255)
           ggCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCa                                                                                                                                   <  1:3378270/148‑1 (MQ=255)
                cAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTc                                                                                                                              >  2:997027/1‑148 (MQ=255)
                   agCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACg                                                                                                                           <  1:1260729/148‑1 (MQ=255)
                   agCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACg                                                                                                                           >  2:3605495/1‑148 (MQ=255)
                        gCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGc                                                                                                                      >  2:2048408/1‑148 (MQ=255)
                         ccTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCg                                                                                                                     >  1:2281027/1‑148 (MQ=255)
                            ctGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTgg                                                                                                                  <  2:2298286/148‑1 (MQ=255)
                            ctGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTgg                                                                                                                  >  2:1013253/1‑148 (MQ=255)
                            ctGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTgg                                                                                                                  <  1:3778706/148‑1 (MQ=255)
                                      aTCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGggcggc                                                                                                        >  1:2869995/1‑148 (MQ=255)
                                       tCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCa                                                                                                       >  2:3362207/1‑148 (MQ=255)
                                        cGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAg                                                                                                      <  2:2754068/148‑1 (MQ=255)
                                          aTAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGAc                                                                                                    >  2:1093995/1‑148 (MQ=255)
                                          aTAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGAc                                                                                                    >  2:2230108/1‑148 (MQ=255)
                                              ccAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGc                                                                                                >  2:1300811/1‑148 (MQ=255)
                                                  aaTTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCgaga                                                                                            >  2:1620212/1‑148 (MQ=255)
                                                    ttCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGaaa                                                                                          >  1:2235247/1‑148 (MQ=255)
                                                     tCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAAc                                                                                         >  2:1468858/1‑148 (MQ=255)
                                                        cATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTc                                                                                      >  1:773726/1‑148 (MQ=255)
                                                           gggTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTcgcg                                                                                   >  2:1245139/1‑148 (MQ=255)
                                                               cTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGt                                                                               >  1:2970588/1‑148 (MQ=255)
                                                                 tCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTaa                                                                             >  1:897913/1‑148 (MQ=255)
                                                                 tCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTaa                                                                             >  1:2188171/1‑148 (MQ=255)
                                                                 tCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTaa                                                                             >  1:2749424/1‑148 (MQ=255)
                                                                   gTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAgg                                                                           <  1:2625152/148‑1 (MQ=255)
                                                                   gTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAgg                                                                           >  2:3105747/1‑148 (MQ=255)
                                                                    tGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGa                                                                          >  1:3060251/1‑148 (MQ=255)
                                                                    tGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGa                                                                          <  1:3233286/148‑1 (MQ=255)
                                                                     gCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGAc                                                                         >  2:185389/1‑148 (MQ=255)
                                                                     gCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGAc                                                                         >  1:1833565/1‑148 (MQ=255)
                                                                       ttAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGc                                                                       <  1:2774189/148‑1 (MQ=255)
                                                                        tAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCa                                                                      >  2:593656/1‑148 (MQ=255)
                                                                           aTCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCa                                                                   >  1:2716626/1‑148 (MQ=255)
                                                                           aTCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCa                                                                   >  1:2716612/1‑148 (MQ=255)
                                                                           aTCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCa                                                                   >  2:1333583/1‑148 (MQ=255)
                                                                            tCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCAGGAGGATCGTATCGAAAGGAAACTAACCGCACGGGCAATTTCACGCGTGAGTGGCGAGGGCGGCAGACAGGCCAGAAACGTCGCGCCGTAAGGACGCATCAc                                                                  >  2:2429425/1‑148 (MQ=255)
                                                                             cAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCAcc                                                                 >  1:1470965/1‑148 (MQ=255)
                                                                             cAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCAcc                                                                 >  1:1471445/1‑148 (MQ=255)
                                                                                tAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGc                                                              >  2:1918038/1‑148 (MQ=255)
                                                                                tAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGc                                                              >  2:3008936/1‑148 (MQ=255)
                                                                                 aaaaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTACGGACGCATCACCAGcc                                                             >  2:2453459/1‑148 (MQ=255)
                                                                                 aaaaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGcc                                                             <  1:423374/148‑1 (MQ=255)
                                                                                   aaaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGa                                                           <  2:1185397/148‑1 (MQ=255)
                                                                                    aaGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAt                                                          <  2:802824/148‑1 (MQ=255)
                                                                                     aGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGAtt                                                         >  2:1147790/1‑148 (MQ=255)
                                                                                      ggCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTg                                                        >  1:2560113/1‑148 (MQ=255)
                                                                                      ggCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTg                                                        >  1:82156/1‑148 (MQ=255)
                                                                                       gcgcgcATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGt                                                       >  2:2183350/1‑148 (MQ=255)
                                                                                          cgcATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTcac                                                    >  1:351730/1‑148 (MQ=255)
                                                                                            catcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACaa                                                  <  2:857288/148‑1 (MQ=255)
                                                                                            catcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACaa                                                  >  2:2626261/1‑148 (MQ=255)
                                                                                            catcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACaa                                                  >  2:3590407/1‑148 (MQ=255)
                                                                                            catcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATAGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACaa                                                  >  1:844225/1‑148 (MQ=255)
                                                                                            catcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATAGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACaa                                                  >  1:770397/1‑148 (MQ=255)
                                                                                              tcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTACGGACGCAGCACCAGCCGAGTGTCCCTAAt                                                >  2:3683531/1‑148 (MQ=255)
                                                                                              tcatACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAAt                                                >  2:1697185/1‑148 (MQ=255)
                                                                                               cataccatacTCCGTAACAAATTTCCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                               >  2:63765/1‑148 (MQ=255)
                                                                                               cataccatacTCCGTAACAAATTTCCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                               >  2:64550/1‑148 (MQ=255)
                                                                                               cataccatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATc                                               >  2:3012131/1‑148 (MQ=255)
                                                                                                  accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                            <  2:1411849/148‑1 (MQ=255)
                                                                                                  accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                            <  2:3746256/148‑1 (MQ=255)
                                                                                                  accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                            >  2:179190/1‑148 (MQ=255)
                                                                                                  accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                            >  1:3145394/1‑148 (MQ=255)
                                                                                                  accatacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                            <  2:2259087/148‑1 (MQ=255)
                                                                                                  accatacTCCGTAACAAAGTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCAcc                                            <  1:800206/148‑1 (MQ=255)
                                                                                                      tacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCa                                        <  1:2401906/148‑1 (MQ=255)
                                                                                                      tacTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCa                                        >  2:822958/1‑148 (MQ=255)
                                                                                                        cTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACg                                      >  1:612916/1‑148 (MQ=255)
                                                                                                        cTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACg                                      >  2:646929/1‑148 (MQ=255)
                                                                                                         tCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGc                                     >  1:387341/1‑148 (MQ=255)
                                                                                                          ccGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTCAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGcc                                    >  2:3413399/1‑148 (MQ=255)
                                                                                                           cGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCa                                   <  2:386872/148‑1 (MQ=255)
                                                                                                              aaCATATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGTGAAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGa                                <  1:1692067/148‑1 (MQ=255)
                                                                                                               aCAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAt                               >  2:602329/1‑148 (MQ=255)
                                                                                                                cAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtc                              >  2:422922/1‑148 (MQ=255)
                                                                                                                cAAATTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtc                              >  2:788393/1‑148 (MQ=255)
                                                                                                                 aaaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcg                             >  1:1050687/1‑148 (MQ=255)
                                                                                                                 aaaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcg                             <  1:202253/148‑1 (MQ=255)
                                                                                                                  aaTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgt                            <  2:1647603/148‑1 (MQ=255)
                                                                                                                   aTTACCTGGAGGATGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGAtcgtc                           >  2:2947124/1‑148 (MQ=255)
                                                                                                                           gaggaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTcgc                   >  1:2848063/1‑148 (MQ=255)
                                                                                                                             ggaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGa                 >  1:2652035/1‑148 (MQ=255)
                                                                                                                              gaTGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGaa                >  1:2885295/1‑148 (MQ=255)
                                                                                                                                tGGTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATc              >  2:2698087/1‑148 (MQ=255)
                                                                                                                                 ggTATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCa             <  2:3457901/148‑1 (MQ=255)
                                                                                                                                   tATCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGt           <  2:2851680/148‑1 (MQ=255)
                                                                                                                                    aTCGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTc          >  1:3460747/1‑148 (MQ=255)
                                                                                                                                      cGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGc        >  1:1953519/1‑148 (MQ=255)
                                                                                                                                      cGCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGc        >  1:22771/1‑148 (MQ=255)
                                                                                                                                      cGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGc        >  1:1677483/1‑148 (MQ=255)
                                                                                                                                       gCAAGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGcc       <  2:3715573/148‑1 (MQ=255)
                                                                                                                                          aGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTa    <  1:3698260/148‑1 (MQ=255)
                                                                                                                                          aGGAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTa    >  2:1024460/1‑148 (MQ=255)
                                                                                                                                            gAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTAcc  >  1:1691835/1‑148 (MQ=255)
                                                                                                                                            gAAACTAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTAcc  >  1:3583121/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
TCGTTCCACAGGGCGACAGAGCAGGCCTCTGTCGCGGTATCGATAGCCAGAATTCGCATGGGTCTTCGTGCTTAGATCAATAAAAAGGCGCGCATCATACCATACTCCGTAACAAATTACCTGGAGGATGGTATCGCAAGGAAACGAACCGCACGGGCAATGTCACGGGTGCGTGGCGCGGGCGGCAGACTGGCGAGAAACGTCGCGCCGTAAGGACGCATCACCAGCCGATTGTCACAAATCACCAGCACGCCACGATCGTCGGCGTCGCGAATCAGTCGCCCTACC  >  CP009273/1885466‑1885753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑