Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,962,923 C→A 100% M233I (ATG→ATT)  cheR ← chemotaxis regulator, protein‑glutamate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,962,9230CA100.0% 659.4 / NA 200M233I (ATG→ATT) cheRchemotaxis regulator, protein‑glutamate methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (98/102);  total (98/102)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

CATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATG  >  CP009273/1962778‑1963067
                                                                                                                                                 |                                                                                                                                                
cATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAAt                                                                                                                                                <  2:2091926/148‑1 (MQ=255)
    cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGACGTAGATAATGACg                                                                                                                                            >  2:628769/1‑148 (MQ=255)
    cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                            <  2:2642602/148‑1 (MQ=255)
    cacCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACg                                                                                                                                            <  1:1637199/148‑1 (MQ=255)
      ccGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGtt                                                                                                                                          >  2:1988417/1‑148 (MQ=255)
         tCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACg                                                                                                                                       <  1:2195207/148‑1 (MQ=255)
         tCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACg                                                                                                                                       <  1:2191110/148‑1 (MQ=255)
            gACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACa                                                                                                                                    >  1:2639105/1‑148 (MQ=255)
               cACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaa                                                                                                                                 <  2:2262909/148‑1 (MQ=255)
                 cGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACagaaga                                                                                                                               <  1:988783/148‑1 (MQ=255)
                  gCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGAt                                                                                                                              <  1:3249443/148‑1 (MQ=255)
                     gCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCACTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGc                                                                                                                           <  2:51624/148‑1 (MQ=255)
                     gCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGc                                                                                                                           <  2:898940/148‑1 (MQ=255)
                       gTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAt                                                                                                                         <  1:1566940/148‑1 (MQ=255)
                       gTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAt                                                                                                                         <  2:2876108/148‑1 (MQ=255)
                       gTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAt                                                                                                                         <  1:372598/148‑1 (MQ=255)
                          aaGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaa                                                                                                                      <  1:1998555/148‑1 (MQ=255)
                           aGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaaa                                                                                                                     <  2:2627384/148‑1 (MQ=255)
                           aGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCaaa                                                                                                                     <  2:710972/148‑1 (MQ=255)
                            gcggcgCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAAc                                                                                                                    <  2:2279801/148‑1 (MQ=255)
                             cggcgCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAAACCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCGGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACg                                                                                                                   >  1:1064699/1‑148 (MQ=255)
                               gcgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGc                                                                                                                 <  2:2389602/148‑1 (MQ=255)
                                cgcTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGcc                                                                                                                >  2:207316/1‑148 (MQ=255)
                                    cAAGGTGGCTAAATTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCgg                                                                                                            <  1:3440271/148‑1 (MQ=255)
                                     aaGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGc                                                                                                           >  2:285371/1‑148 (MQ=255)
                                       ggTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCAc                                                                                                         >  2:1963909/1‑148 (MQ=255)
                                           gCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtg                                                                                                     <  1:1842126/148‑1 (MQ=255)
                                           gCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGGCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtg                                                                                                     <  1:2772791/148‑1 (MQ=255)
                                            cTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGgtgt                                                                                                    >  2:648252/1‑148 (MQ=255)
                                              aaaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                  >  1:455374/1‑148 (MQ=255)
                                              aaaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTAc                                                                                                  >  1:2912962/1‑148 (MQ=255)
                                               aaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCACACGGCCCCGGCACGGTGTACt                                                                                                 <  2:829127/148‑1 (MQ=255)
                                               aaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACt                                                                                                 >  2:563364/1‑148 (MQ=255)
                                               aaGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACt                                                                                                 >  1:1661187/1‑148 (MQ=255)
                                                tgTTTTCAGAGTGACCCGCAAACAGAAATCCGTCGGTTTTACGGAGCGTAACAAAGCGGCGAAAAATCTCCTGCTGGGTTGTTTGATCGAAGTATATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTg                                                                                                <  1:396077/147‑1 (MQ=255)
                                                  ttttCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGtt                                                                                              >  2:3091683/1‑148 (MQ=255)
                                                  ttttCAGAGTCACCCCCAAACAGCATTCCGTCGGTTTTATGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTATTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGtt                                                                                              <  1:2575187/148‑1 (MQ=255)
                                                   tttCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGttt                                                                                             >  2:943359/1‑148 (MQ=255)
                                                      cAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATAGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGc                                                                                          <  1:1703301/148‑1 (MQ=255)
                                                        gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGcc                                                                                         <  1:2819348/147‑1 (MQ=255)
                                                        gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGcc                                                                                         >  2:2819348/1‑147 (MQ=255)
                                                        gagTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCa                                                                                        >  2:1587392/1‑148 (MQ=255)
                                                            gACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAg                                                                                    <  2:219375/148‑1 (MQ=255)
                                                             aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGa                                                                                   >  2:2503373/1‑148 (MQ=255)
                                                             aCCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGa                                                                                   >  1:1902724/1‑148 (MQ=255)
                                                                cGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                >  2:316213/1‑148 (MQ=255)
                                                                cGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTc                                                                                >  2:1088564/1‑148 (MQ=255)
                                                                  cAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAACAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAg                                                                              <  1:1002176/148‑1 (MQ=255)
                                                                    aaCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCg                                                                            >  1:831742/1‑148 (MQ=255)
                                                                    aaCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCg                                                                            >  1:2744920/1‑148 (MQ=255)
                                                                     aCAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCgg                                                                           <  1:1076930/148‑1 (MQ=255)
                                                                         cAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCa                                                                       >  2:1354215/1‑148 (MQ=255)
                                                                         cAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCa                                                                       >  2:3549076/1‑148 (MQ=255)
                                                                           aTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                     <  1:2053921/148‑1 (MQ=255)
                                                                           aTCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaa                                                                     <  2:3679795/148‑1 (MQ=255)
                                                                            tCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCaaaa                                                                    >  2:2943855/1‑148 (MQ=255)
                                                                                 cGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTATATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAAc                                                               <  1:371623/148‑1 (MQ=255)
                                                                                   gttttACGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAACAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                             <  1:1311433/146‑1 (MQ=255)
                                                                                   ggTTTAAGGAGCGGAACAACGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                             <  2:1780556/148‑1 (MQ=255)
                                                                                   ggTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACAt                                                             <  1:260190/148‑1 (MQ=255)
                                                                                    gTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATa                                                            <  2:3352379/148‑1 (MQ=255)
                                                                                      ttAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGt                                                          >  2:757625/1‑148 (MQ=255)
                                                                                       tAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGtt                                                         <  2:2350571/148‑1 (MQ=255)
                                                                                          ggAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGc                                                      >  2:679602/1‑148 (MQ=255)
                                                                                             gCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAg                                                   <  2:1567071/148‑1 (MQ=255)
                                                                                                  aCAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCt                                              >  2:1526154/1‑148 (MQ=255)
                                                                                                   cAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTg                                             >  2:20665/1‑148 (MQ=255)
                                                                                                       gcggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgc                                         >  2:3389136/1‑148 (MQ=255)
                                                                                                        cggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCACACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgca                                        <  2:849261/148‑1 (MQ=255)
                                                                                                        cggcgCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGacgca                                        <  1:2503373/148‑1 (MQ=255)
                                                                                                             cAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGt                                   <  2:1817485/148‑1 (MQ=255)
                                                                                                                aaTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTAcc                                >  2:959214/1‑148 (MQ=255)
                                                                                                                 aTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCa                               >  2:476298/1‑148 (MQ=255)
                                                                                                                 aTCTCCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCa                               >  2:476229/1‑148 (MQ=255)
                                                                                                                    tcCTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCAACAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGcc                            <  1:695456/148‑1 (MQ=255)
                                                                                                                     ccTGCTGGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGccc                           <  1:3150547/148‑1 (MQ=255)
                                                                                                                      ctgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCt                          <  1:2122448/148‑1 (MQ=255)
                                                                                                                       tgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCtt                         <  1:3580023/148‑1 (MQ=255)
                                                                                                                       tgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCtt                         <  1:1363279/148‑1 (MQ=255)
                                                                                                                       tgctgGGTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCtt                         >  2:173945/1‑148 (MQ=255)
                                                                                                                           gggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATg                     <  2:1788068/148‑1 (MQ=255)
                                                                                                                           gggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATg                     >  1:334981/1‑148 (MQ=255)
                                                                                                                            ggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                    >  2:3021093/1‑148 (MQ=255)
                                                                                                                            ggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                    >  1:2403689/1‑148 (MQ=255)
                                                                                                                            ggTAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGc                    >  2:1682864/1‑148 (MQ=255)
                                                                                                                              tAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCgg                  >  2:1754544/1‑148 (MQ=255)
                                                                                                                              tAGTTTGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCgg                  >  1:3362498/1‑148 (MQ=255)
                                                                                                                                 tttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGccc               >  1:2767353/1‑148 (MQ=255)
                                                                                                                                  ttGATCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGcccc              >  2:1450162/1‑148 (MQ=255)
                                                                                                                                     aTCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATCGTTCGCCAGCGCCTGACGCACGCGTCCCCGCCCTTCATGCGGCCCCGTc           >  2:2474698/1‑148 (MQ=255)
                                                                                                                                      tCGAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTcc          >  2:994847/1‑148 (MQ=255)
                                                                                                                                        gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt        <  1:3108809/148‑1 (MQ=255)
                                                                                                                                        gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt        >  2:2721573/1‑148 (MQ=255)
                                                                                                                                        gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt        <  1:2239122/148‑1 (MQ=255)
                                                                                                                                        gAAGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCt        <  2:1064699/148‑1 (MQ=255)
                                                                                                                                          aGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg      >  1:2317157/1‑148 (MQ=255)
                                                                                                                                          aGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg      >  1:2316646/1‑148 (MQ=255)
                                                                                                                                          aGTAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCg      >  2:790392/1‑148 (MQ=255)
                                                                                                                                            tAGATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCa    <  1:1246443/148‑1 (MQ=255)
                                                                                                                                              gATAATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATg  >  1:1167425/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATACACCGTCTGACCACGCAGCGTGAAGCGGCGCTCAAGGTGGCTAAAGTTTTCAGAGTGACCCGCAAACAGCAATCCGTCGGGTTTAAGGAGCGGAACAAAGCGGCGCAAAATCTCCTGCTGGGTAGTTTGATCGAAGTAGATCATGACGTTACGACAGAAGATCGCATCAAACGGCCCCGGCACGGTGTACTGTTTCGCCAGTAGATTCAGCGGGGCAAAATCAACATAGTTCGCCAGCTCCTGACGCACGCGTACCAGCCCTTCATGCGGCCCCGTCCCTCGCATG  >  CP009273/1962778‑1963067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑