Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,002,354 C→A 100% L199L (CTC→CTA)  yedE → UPF0394 family sulfur transport domain‑containing inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,002,3540CA95.4% 503.3 / 15.3 210L199L (CTC→CTA) yedEUPF0394 family sulfur transport domain‑containing inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (3/7);  new base A (96/99);  total (104/106)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 210 total aligned reads displayed.

TTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG  >  CP009273/2002208‑2002497
                                                                                                                                                  |                                                                                                                                                
 ttGCCATCGCGACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAgg                                                                                                                                                >  2:1642214/1‑148 (MQ=255)
 ttGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAgg                                                                                                                                                >  2:309809/1‑148 (MQ=255)
  tGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAggg                                                                                                                                               <  2:3606557/148‑1 (MQ=255)
      aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGc                                                                                                                                           <  2:1476954/148‑1 (MQ=255)
      aTCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGc                                                                                                                                           <  2:1477106/148‑1 (MQ=255)
          ccACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGt                                                                                                                                       <  1:534727/148‑1 (MQ=255)
           cACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTc                                                                                                                                      <  2:1216214/148‑1 (MQ=255)
            aCTGCCATTGGTTCATGGTGTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCt                                                                                                                                     <  2:491045/148‑1 (MQ=255)
             cTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtt                                                                                                                                    >  2:1287674/1‑148 (MQ=255)
              tGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   >  2:1015809/1‑148 (MQ=255)
              tGCCATTGGTTCATAGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttt                                                                                                                                   <  2:3515868/148‑1 (MQ=255)
               gCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCtttt                                                                                                                                  <  1:1326909/148‑1 (MQ=255)
                ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCttttt                                                                                                                                 <  2:3279728/148‑1 (MQ=255)
                ccATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGAGGGATGCTGGTCttttt                                                                                                                                 >  1:1176605/1‑148 (MQ=255)
                 cATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTc                                                                                                                                >  2:2499600/1‑148 (MQ=255)
                 cATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTc                                                                                                                                <  2:1869459/148‑1 (MQ=255)
                  aTTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTCTAGGGATGCGGGTCTTTTTCg                                                                                                                               >  2:3325541/1‑148 (MQ=255)
                   ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                              <  1:2810202/148‑1 (MQ=255)
                   ttGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCgg                                                                                                                              >  2:734300/1‑148 (MQ=255)
                     ggTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCTGCa                                                                                                                            <  2:2823422/148‑1 (MQ=255)
                       ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTGGAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          >  1:3656113/1‑148 (MQ=255)
                       ttCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATg                                                                                                                          <  1:1434806/148‑1 (MQ=255)
                        tCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgc                                                                                                                         >  2:2206072/1‑148 (MQ=255)
                        tCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgc                                                                                                                         >  2:548617/1‑148 (MQ=255)
                           tGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctgg                                                                                                                      >  2:2760090/1‑148 (MQ=255)
                             gTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATgctgggc                                                                                                                    <  2:671106/148‑1 (MQ=255)
                              tttGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATgctgggct                                                                                                                   >  1:1923091/1‑148 (MQ=255)
                                   cgcgcgcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTCTAGGGGTGGTGGTGTTTTTCGGGATGGTgggcggggcg                                                                                                              >  1:1015661/1‑148 (MQ=255)
                                        gcTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCgctgct                                                                                                         <  2:135875/148‑1 (MQ=255)
                                         cTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTc                                                                                                        >  2:755974/1‑148 (MQ=255)
                                         cTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTc                                                                                                        >  1:1855101/1‑148 (MQ=255)
                                         cTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTATGCCGCAGAACATCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGGTGCTGGTATTTTTGGGCATGCTGGGGTTGGCGCTGCTc                                                                                                        >  1:3745739/1‑148 (MQ=255)
                                          tttACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTca                                                                                                       <  1:2588555/148‑1 (MQ=255)
                                             aCCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAg                                                                                                    <  1:838050/148‑1 (MQ=255)
                                               ccTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCg                                                                                                  <  2:2822119/148‑1 (MQ=255)
                                                   ctgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGa                                                                                              <  2:2720017/148‑1 (MQ=255)
                                                    tgcCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTGTAGGGCTGGTGGTGTTTTTCGGCATGGTGGGCTGGGCGCTGGTCACAGCGCTGaa                                                                                             >  1:1071703/1‑148 (MQ=255)
                                                        cATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGGTGGGCTGGGCGCTGCTCACAGCGATGaaccaa                                                                                         >  2:311027/1‑148 (MQ=255)
                                                         aTCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGaaccaac                                                                                        <  1:2454709/148‑1 (MQ=255)
                                                            ttCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCa                                                                                     >  2:1840244/1‑148 (MQ=255)
                                                             tCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaa                                                                                    >  2:3814286/1‑148 (MQ=255)
                                                              ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCcaa                                                                                   >  1:1594092/1‑148 (MQ=255)
                                                              ccGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCaaa                                                                                   <  1:426475/148‑1 (MQ=255)
                                                                gTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACt                                                                                 <  2:2072164/148‑1 (MQ=255)
                                                                gTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACt                                                                                 >  2:3774369/1‑148 (MQ=255)
                                                                 tATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGGTCGTCTAGGGATGCGGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAGCCAACCCAAACTg                                                                                >  2:1711813/1‑148 (MQ=255)
                                                                  aTTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgg                                                                               <  1:2194416/148‑1 (MQ=255)
                                                                   gtCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTggg                                                                              <  1:985346/147‑1 (MQ=255)
                                                                    tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGCTGCTGGTCTTTTTGGGCCTGGTGGGGGGGGCGCTGCTCACCGCGATGAACCAACCCAAACTgggg                                                                             >  2:1325625/1‑148 (MQ=255)
                                                                    tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgggg                                                                             >  2:1030322/1‑148 (MQ=255)
                                                                    tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTgggg                                                                             >  2:2144611/1‑148 (MQ=255)
                                                                    tCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGGGTAGGGGTGCGGGTGTTTTTGGGCCTGCGGGGGGGGGCGCTGCTCACAGCGATGAACCAACCCCAACAgggg                                                                             >  1:752820/1‑148 (MQ=255)
                                                                      ccGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGGTGGGGTGCGGGGGTTTTTGGGGCTGGGGGGGGGGGGGCGGGTCCCAGCGATGAACCAACCCCAACAgggggg                                                                           >  1:22925/1‑146 (MQ=255)
                                                                       cGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTGTAGGGATGCTGGTGTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑g                                                                         >  1:1224766/1‑148 (MQ=255)
                                                                       cGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑g                                                                         >  1:3177181/1‑148 (MQ=255)
                                                                          gAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGTCTAGGGCTGGTGGTATTTTTCGGCATGCTGGGGTGGGCGCTGCTCACATCGATGAACCAAACCAAACAGGGGCT‑GGCa                                                                      >  1:2985866/1‑148 (MQ=255)
                                                                          gAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAGGCTGGTCTTTTTCGGCATGGTGGGCGGGGCGCTGCTCACCCCGATGAACCAACCCCACCAGGGGCT‑GGCa                                                                      >  1:3290501/1‑148 (MQ=255)
                                                                            aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTGGGCTAGGGGTGCGGGTCTTTTTAGGCATGCTGGGGGGGGGGCGGCTCACAGCGCTGAACCAAACCAAACAGGGGGT‑GGCaaa                                                                    >  1:3205484/1‑147 (MQ=255)
                                                                            aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTGTTTTTAGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCCAt                                                                    >  1:1985400/1‑148 (MQ=255)
                                                                            aaaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGTCTAGGGGTGCTGGTGTTTTTGGGCATGCTGGGCTGGGCGCTGCCCACAGCGATGAACCAACCCACACTGGGGCT‑GGCCAt                                                                    >  1:2932222/1‑148 (MQ=255)
                                                                             aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGGTGGTCTTTTTAGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATg                                                                   >  2:1107653/1‑148 (MQ=255)
                                                                             aaTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATg                                                                   >  2:2452599/1‑148 (MQ=255)
                                                                              aTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGc                                                                  <  1:528635/148‑1 (MQ=255)
                                                                              aTGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGc                                                                  <  1:1436514/148‑1 (MQ=255)
                                                                               tGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGcg                                                                 >  2:708475/1‑147 (MQ=255)
                                                                                gCAGAAAGTGTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTg                                                                <  2:3482413/148‑1 (MQ=255)
                                                                                 cAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGt                                                               <  1:505435/148‑1 (MQ=255)
                                                                                  aGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGtt                                                              <  1:3391839/148‑1 (MQ=255)
                                                                                    aaaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGGGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTg                                                            >  2:1098638/1‑148 (MQ=255)
                                                                                     aaGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTGGGGGTGGGGGTGTTTTTGGGGATGGTGGGGGGGGGGCGGCTCACAGCGATGTACCAACCCAAACAGGGGCTGGGCAAT‑CTGttttg                                                           >  1:1340640/1‑146 (MQ=255)
                                                                                        tttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGt                                                        <  1:191637/148‑1 (MQ=255)
                                                                                         ttCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTAGGGAAGGGATGGTGGTCTTTTTCGGCATTCTGGGCAGGGCGCTGCTCACAGCCATGCACCAACCCAAACTGGGGGT‑GGCAAAGCGGTTTGGCGTc                                                       >  2:146840/1‑148 (MQ=255)
                                                                                                 ccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTgg                                               <  2:2057235/148‑1 (MQ=255)
                                                                                                 ccTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTgg                                               <  2:1438498/148‑1 (MQ=255)
                                                                                                  cTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGt                                              <  2:3745580/148‑1 (MQ=255)
                                                                                                  cTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGt                                              <  2:1855101/148‑1 (MQ=255)
                                                                                                   tCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTGGTGTAGGGTTGCTGGTGTTTTTGGGCATTCTGGGGGGGGCGCGGCTCACAGCGATGAAACAAACCAAACAGGGGGT‑GGCGATTCTGTTTTGGGTCGGCTTTGGtt                                             >  1:3608864/1‑148 (MQ=255)
                                                                                                    cACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGttt                                            <  1:3571272/148‑1 (MQ=255)
                                                                                                     aCCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTGTTCGTCGAGGGATGCGGGGCTTTTTGGGGATGCTGGGCGGGGGGCGGCTCACAGCGCTGCAACAACCCAAACGGGGGCG‑GGCAATGCGGGTTGGGGTCGGCGTTGGgttt                                           >  1:453739/1‑147 (MQ=255)
                                                                                                       cGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGAGGCGGGTCATTTTGGGCATGCTGGGGTGGGCGCTGCTCACAGCGATGAACCAACCCCAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTaat                                         >  2:2596702/1‑146 (MQ=255)
                                                                                                             cGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGa                                   <  1:3278547/148‑1 (MQ=255)
                                                                                                             cGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCGAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGa                                   <  2:73075/148‑1 (MQ=255)
                                                                                                                aaaaaCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACg                                <  2:3656756/148‑1 (MQ=255)
                                                                                                                    aCCGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTgcg                            <  1:309809/148‑1 (MQ=255)
                                                                                                                     ccGGATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGGTTGGCGTCGGCGTTGGTTTACTGATTGAACGTgcgc                           >  2:2755717/1‑148 (MQ=255)
                                                                                                                       ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGGAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                         <  1:322863/148‑1 (MQ=255)
                                                                                                                       ggATCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCaa                         <  1:1138103/148‑1 (MQ=255)
                                                                                                                          tCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAAta                      >  2:1593369/1‑147 (MQ=255)
                                                                                                                          tCAGGCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTAGGCATGCTGGGCTGGGCGCTGCTCACACCGATGAACCAAACCAAACTGGGGCT‑GGCAAATCTGTTTGGCGTCGGCGTTTGTTTACTGATTGAAAGTGCGCaaaaa                      >  2:3584314/1‑146 (MQ=255)
                                                                                                                            aGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTg                    <  2:231328/148‑1 (MQ=255)
                                                                                                                             ggCGCGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGc                   <  1:157625/148‑1 (MQ=255)
                                                                                                                              gcgcGGCGTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCt                  <  1:3774369/148‑1 (MQ=255)
                                                                                                                                 cggcgTCGTTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTa               <  1:1840244/148‑1 (MQ=255)
                                                                                                                                   gcgTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACt             <  2:1923091/148‑1 (MQ=255)
                                                                                                                                    cgtcgtTTTCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACtt            <  2:2663974/148‑1 (MQ=255)
                                                                                                                                         ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg       <  2:3656113/148‑1 (MQ=255)
                                                                                                                                         ttttCGTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCg       >  1:1348689/1‑148 (MQ=255)
                                                                                                                                          tttCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGt      >  2:2569520/1‑148 (MQ=255)
                                                                                                                                              gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcg  <  1:3640470/148‑1 (MQ=255)
                                                                                                                                              gTCTAGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCcg  <  2:2812828/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TTTGCCATCGCCACTGCCATTGGTTCATGGTTTGGCGCGCGCTTTACCCTGCTGCCCATCTTCCGTATTCCCGTGAAAATGCAGAAAGTTTCTGCCGCCTCACCGCTGACGCAAAAACCGGATCAGGCGCGGCGTCGTTTTCGTCTCGGGATGCTGGTCTTTTTCGGCATGCTGGGCTGGGCGCTGCTCACAGCGATGAACCAACCCAAACTGGGGCT‑GGCAATGCTGTTTGGCGTCGGCTTTGGTTTACTGATTGAACGTGCGCAAATCTGCTTTACTTCAGCGTTCCG  >  CP009273/2002208‑2002497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑