Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,049,111 C→A 100% T190T (ACC→ACA)  amn → AMP nucleosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,049,1110CA97.8% 702.0 / 5.7 228T190T (ACC→ACA) amnAMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (104/119);  total (104/124)
Fisher's exact test for biased strand distribution p-value = 6.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 228 total aligned reads displayed.

TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255
                                                                                                                                                 |                                                                                                                                                
      gCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGcc                                                                                                                                          >  1:1350572/1‑148 (MQ=255)
          cTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGtt                                                                                                                                      >  2:3447065/1‑148 (MQ=255)
            tCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTCTCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGa                                                                                                                                    >  2:1237596/1‑148 (MQ=255)
              ccGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAAc                                                                                                                                  >  1:2553352/1‑148 (MQ=255)
                 acaacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                               >  2:2018635/1‑148 (MQ=255)
                 acaacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                               <  2:953802/148‑1 (MQ=255)
                 acaacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtt                                                                                                                               <  2:334344/148‑1 (MQ=255)
                   aacaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACAtttt                                                                                                                             >  1:2896956/1‑148 (MQ=255)
                    acaGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTc                                                                                                                            <  1:831099/148‑1 (MQ=255)
                        aaCTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTTCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGcc                                                                                                                        >  1:3349597/1‑148 (MQ=255)
                          cTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGt                                                                                                                      >  2:1485660/1‑148 (MQ=255)
                               gcAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTc                                                                                                                 >  2:2125170/1‑148 (MQ=255)
                                cAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCt                                                                                                                <  2:802119/148‑1 (MQ=255)
                                 aatttGGAGATGAAACTTCAGTCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCTCCGCGTCGATTTTTCCCTCGCACTGTTGCGCCATTATACAGGTACGACATTTGAACATTTTCAGCCGTTCGTCtt                                                                                                               <  1:364326/145‑1 (MQ=255)
                                   aTTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGt                                                                                                             <  2:1016127/148‑1 (MQ=255)
                                   aTTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGt                                                                                                             <  2:815654/148‑1 (MQ=255)
                                    ttGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGtt                                                                                                            >  1:1916577/1‑148 (MQ=255)
                                     tGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGttt                                                                                                           >  2:2723472/1‑148 (MQ=255)
                                      ggCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTa                                                                                                          <  2:3434234/148‑1 (MQ=255)
                                      ggCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTa                                                                                                          <  2:3540435/148‑1 (MQ=255)
                                          aTGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCaa                                                                                                      <  1:532044/148‑1 (MQ=255)
                                          aTGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCaa                                                                                                      <  2:987477/148‑1 (MQ=255)
                                           tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAAc                                                                                                     <  2:2776310/148‑1 (MQ=255)
                                           tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAAc                                                                                                     >  2:3148804/1‑148 (MQ=255)
                                           tGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTACCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAAc                                                                                                     >  2:1098878/1‑148 (MQ=255)
                                            gAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACt                                                                                                    >  1:1032090/1‑148 (MQ=255)
                                                cTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacac                                                                                                <  1:1002824/148‑1 (MQ=255)
                                                 tGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTacaca                                                                                               >  2:1896585/1‑148 (MQ=255)
                                                     gACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGtt                                                                                           <  1:3299936/148‑1 (MQ=255)
                                                      aCGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTa                                                                                          >  2:1599207/1‑148 (MQ=255)
                                                        ggCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtg                                                                                        <  2:2162787/148‑1 (MQ=255)
                                                        ggCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTAtg                                                                                        <  2:3781907/148‑1 (MQ=255)
                                                           aTTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTgg                                                                                     >  2:1267038/1‑148 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                    >  2:3175465/1‑148 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                    >  2:3773717/1‑148 (MQ=255)
                                                            tttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGa                                                                                    <  1:312258/148‑1 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  2:3394932/1‑148 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  1:2986211/1‑148 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  2:1750747/1‑148 (MQ=255)
                                                             ttATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGAt                                                                                   >  2:1159425/1‑148 (MQ=255)
                                                               atcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGa                                                                                 <  1:729720/148‑1 (MQ=255)
                                                               atcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGa                                                                                 <  2:670443/148‑1 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                <  2:3273590/148‑1 (MQ=255)
                                                                tcatcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGaa                                                                                >  1:3253085/1‑148 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAt                                                                               >  2:760022/1‑148 (MQ=255)
                                                                 catcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAt                                                                               <  2:3727978/148‑1 (MQ=255)
                                                                   tcCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGAGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATAAAGGTACGCCAGTTGAACATTTTCAGCCGGTCGTCTTGTTTACCAACTAAACACGTTATGTGGATGTAttc                                                                             >  2:1367502/1‑148 (MQ=255)
                                                                    ccAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcg                                                                            <  1:2902991/148‑1 (MQ=255)
                                                                      aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          <  1:3752392/148‑1 (MQ=255)
                                                                      aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          >  2:242218/1‑148 (MQ=255)
                                                                      aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttagtt                                                                          >  1:3577054/1‑148 (MQ=255)
                                                                      aaCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGGTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgtt                                                                          >  1:988142/1‑148 (MQ=255)
                                                                       aCTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttc                                                                         >  2:2127068/1‑148 (MQ=255)
                                                                        cTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAAttcgttcg                                                                        <  2:1659391/148‑1 (MQ=255)
                                                                         tGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATtcgttcgt                                                                       <  2:1427053/148‑1 (MQ=255)
                                                                           aaTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTg                                                                     <  2:2704009/148‑1 (MQ=255)
                                                                           aaTTCTCCCCGCGATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTg                                                                     <  1:116449/148‑1 (MQ=255)
                                                                            aTTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgg                                                                    <  1:2420896/148‑1 (MQ=255)
                                                                             ttCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                   <  2:1030012/148‑1 (MQ=255)
                                                                             ttCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTggg                                                                   <  1:3755261/148‑1 (MQ=255)
                                                                              tctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgggg                                                                  >  1:472385/1‑148 (MQ=255)
                                                                              tctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgggg                                                                  <  1:2163896/148‑1 (MQ=255)
                                                                              tctcCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTgggg                                                                  >  2:1046099/1‑148 (MQ=255)
                                                                                 ccccGCTATCGCATTTTGAGGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATg                                                               <  2:2929672/148‑1 (MQ=255)
                                                                                  cccGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGc                                                              >  1:783462/1‑148 (MQ=255)
                                                                                     gCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGc                                                           <  1:570181/148‑1 (MQ=255)
                                                                                     gCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGc                                                           <  1:129814/148‑1 (MQ=255)
                                                                                      cTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTGCGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                          <  1:3397580/148‑1 (MQ=255)
                                                                                      cTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGcc                                                          >  2:2929873/1‑148 (MQ=255)
                                                                                        aTCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAg                                                        >  2:2621577/1‑148 (MQ=255)
                                                                                          cGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCAGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGAt                                                      <  2:2553352/148‑1 (MQ=255)
                                                                                             aTTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCt                                                   <  1:2215458/148‑1 (MQ=255)
                                                                                               tttGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCg                                                 <  2:910075/148‑1 (MQ=255)
                                                                                               tttGATGCGCGCCGCGTCGATTTTTCCCGCGCACGGTTGGGCCATTATACAGGTACGCCAGTTGAACATTTTAAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTATTTCGTTGGGGATGGAGCCAGATCCACg                                                 >  1:993898/1‑148 (MQ=255)
                                                                                                 tGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGAt                                               <  1:413222/148‑1 (MQ=255)
                                                                                                  gATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATc                                              <  2:419260/148‑1 (MQ=255)
                                                                                                   aTGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATcc                                             >  1:1650207/1‑148 (MQ=255)
                                                                                                      cgcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGa                                          <  2:182915/148‑1 (MQ=255)
                                                                                                       gcgcCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGAt                                         <  1:2012812/148‑1 (MQ=255)
                                                                                                          ccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGt                                      <  1:162964/148‑1 (MQ=255)
                                                                                                          ccgcGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGt                                      <  1:765261/148‑1 (MQ=255)
                                                                                                           cgcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTc                                     <  1:2929557/148‑1 (MQ=255)
                                                                                                            gcgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTcc                                    <  1:2434771/148‑1 (MQ=255)
                                                                                                             cgTCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTccc                                   <  2:566155/148‑1 (MQ=255)
                                                                                                             cgTCGATTTTTACCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTAGTCGTGTTTACCAACTACACACCGTATGTGGATTAATTCGTTAGTTGGGGATGCAGCCACATCCTCGATCCAGATAGTccc                                   >  1:1458977/1‑148 (MQ=255)
                                                                                                               tCGATTTTTCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATATTCCCTa                                 >  2:2859929/1‑148 (MQ=255)
                                                                                                                      ttCCCTCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGcc                          <  1:2125170/148‑1 (MQ=255)
                                                                                                                           tCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTc                     <  1:1653372/148‑1 (MQ=255)
                                                                                                                           tCGCACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTc                     <  1:987695/148‑1 (MQ=255)
                                                                                                                              cACGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtg                  >  1:1740278/1‑148 (MQ=255)
                                                                                                                                cGGTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTtgtg                <  1:1237596/148‑1 (MQ=255)
                                                                                                                                 ggTTGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGc               <  1:1092555/148‑1 (MQ=255)
                                                                                                                                   ttGCGCCATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTg             >  2:237284/1‑148 (MQ=255)
                                                                                                                                        ccATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCggg        <  2:2995035/148‑1 (MQ=255)
                                                                                                                                         cATTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGa       >  1:2702307/1‑148 (MQ=255)
                                                                                                                                          aTTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGaa      <  2:1199154/148‑1 (MQ=255)
                                                                                                                                          aTTATACAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGaa      <  2:1914247/148‑1 (MQ=255)
                                                                                                                                            tataCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACt    >  1:197185/1‑148 (MQ=255)
                                                                                                                                              taCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTgg  <  2:486422/148‑1 (MQ=255)
                                                                                                                                              taCAGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTgg  <  1:789810/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTATACCGGTACGCCAGTTGAACATTTTCAGCCGTTCGTCTTGTTTACCAACTACACACGTTATGTGGATGAATTCGTTCGTTGGGGATGCAGCCAGATCCTCGATCCTGATAGTCCCTACATTGCCCTTTCTTGTGCTGGCGGGAACTGG  >  CP009273/2048966‑2049255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑