Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,051,691 G→T 7.7% S494* (TCG→TAG)  yeeO ← putative multdrug exporter, MATE family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,051,6910GT 7.7% 912.5 / 31.4 247S494* (TCG→TAG) yeeOputative multdrug exporter, MATE family
Reads supporting (aligned to +/- strand):  ref base G (100/128);  new base T (11/8);  total (111/136)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 247 total aligned reads displayed.

TTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCC  >  CP009273/2051546‑2051835
                                                                                                                                                 |                                                                                                                                                
   aCAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTAcaca                                                                                                                                             <  1:1560156/148‑1 (MQ=255)
    cAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAATTCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                                                                                                                            >  2:1976172/1‑148 (MQ=255)
    cAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                                                                                                                            <  1:627784/148‑1 (MQ=255)
    cAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACaa                                                                                                                                            <  1:2545067/148‑1 (MQ=255)
         cgccgTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGc                                                                                                                                       <  2:2670132/148‑1 (MQ=255)
          gccgTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCt                                                                                                                                      <  2:1512093/148‑1 (MQ=255)
          gccgTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCt                                                                                                                                      <  2:1514277/148‑1 (MQ=255)
           ccgTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCtt                                                                                                                                     >  1:2342804/1‑148 (MQ=255)
              ttCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttt                                                                                                                                  <  2:3134344/148‑1 (MQ=255)
                cTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttttt                                                                                                                                <  2:924715/148‑1 (MQ=255)
                cTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttttt                                                                                                                                <  1:706766/148‑1 (MQ=255)
                 tACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTc                                                                                                                               <  1:1088309/148‑1 (MQ=255)
                 tACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTc                                                                                                                               <  2:1337131/148‑1 (MQ=255)
                  aCCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTca                                                                                                                              <  2:2296945/148‑1 (MQ=255)
                  aCCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTca                                                                                                                              <  2:2297454/148‑1 (MQ=255)
                   ccGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTcac                                                                                                                             >  2:3314678/1‑148 (MQ=255)
                     gACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCacac                                                                                                                           >  1:471984/1‑148 (MQ=255)
                      aCTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACt                                                                                                                          <  2:3161528/148‑1 (MQ=255)
                      aCTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACt                                                                                                                          <  1:204885/148‑1 (MQ=255)
                       cTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACtt                                                                                                                         >  1:2989494/1‑148 (MQ=255)
                       cTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACtt                                                                                                                         <  1:2930503/148‑1 (MQ=255)
                             tACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCgg                                                                                                                   <  1:3044700/148‑1 (MQ=255)
                               cAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCt                                                                                                                 <  1:3560045/148‑1 (MQ=255)
                                      aTCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCg                                                                                                          >  1:796066/1‑148 (MQ=255)
                                      aTCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCg                                                                                                          <  2:2159503/148‑1 (MQ=255)
                                      aTCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCg                                                                                                          <  2:2159856/148‑1 (MQ=255)
                                           gagaACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGt                                                                                                     <  2:3014303/148‑1 (MQ=255)
                                             gaACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTAt                                                                                                   <  2:1133878/148‑1 (MQ=255)
                                              aaCGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTAtt                                                                                                  >  2:123840/1‑148 (MQ=255)
                                              aaCGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGGAtt                                                                                                  >  2:123508/1‑148 (MQ=255)
                                              aaCGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTAtt                                                                                                  >  1:3640465/1‑148 (MQ=255)
                                               aCGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTAttt                                                                                                 <  2:102209/148‑1 (MQ=255)
                                                 gAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTcc                                                                                               <  1:1228415/148‑1 (MQ=255)
                                                   ggCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCAt                                                                                             >  1:3237999/1‑148 (MQ=255)
                                                     cGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAg                                                                                           >  2:2620851/1‑148 (MQ=255)
                                                         tatTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCaa                                                                                       >  1:1080515/1‑148 (MQ=255)
                                                         tatTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCaa                                                                                       >  1:2989802/1‑148 (MQ=255)
                                                            tAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGt                                                                                    <  1:2301914/148‑1 (MQ=255)
                                                            tAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGt                                                                                    <  2:837045/148‑1 (MQ=255)
                                                             aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                   <  1:2279829/148‑1 (MQ=255)
                                                             aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                   <  2:3530379/148‑1 (MQ=255)
                                                             aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                   <  1:1443782/148‑1 (MQ=255)
                                                             aGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTc                                                                                   <  1:2279800/148‑1 (MQ=255)
                                                              gCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTcc                                                                                  <  2:505702/148‑1 (MQ=255)
                                                                  tGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTa                                                                              <  2:555815/148‑1 (MQ=255)
                                                                     ccacccacAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAAcc                                                                           <  1:63726/148‑1 (MQ=255)
                                                                     ccacccacAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAAcc                                                                           <  1:859730/148‑1 (MQ=255)
                                                                          cacaATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCg                                                                      >  1:3399293/1‑148 (MQ=255)
                                                                                  cAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTaaa                                                              >  2:1682153/1‑148 (MQ=255)
                                                                                    aaGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCCGTAAAAc                                                            >  2:2562961/1‑148 (MQ=255)
                                                                                    aaGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAAc                                                            >  2:2707863/1‑148 (MQ=255)
                                                                                     aGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACa                                                           >  1:678123/1‑148 (MQ=255)
                                                                                     aGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACa                                                           >  2:2353795/1‑148 (MQ=255)
                                                                                     aGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACa                                                           >  1:318440/1‑148 (MQ=255)
                                                                                       ccTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGc                                                         <  1:2133492/148‑1 (MQ=255)
                                                                                       ccTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGc                                                         >  2:940461/1‑148 (MQ=255)
                                                                                        cTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGGGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCa                                                        >  2:3773551/1‑148 (MQ=255)
                                                                                        cTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCa                                                        >  2:1882291/1‑148 (MQ=255)
                                                                                        cTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCa                                                        <  1:2229407/148‑1 (MQ=255)
                                                                                          gTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACg                                                      >  1:2255164/1‑148 (MQ=255)
                                                                                          gTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACg                                                      >  1:3747913/1‑148 (MQ=255)
                                                                                           ttttttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACgg                                                     <  2:325141/148‑1 (MQ=255)
                                                                                            tttttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGc                                                    <  1:2817065/148‑1 (MQ=255)
                                                                                             ttttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGcc                                                   >  1:2726648/1‑148 (MQ=255)
                                                                                               ttAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCg                                                 >  1:1989328/1‑148 (MQ=255)
                                                                                                 aaaTTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGca                                               <  2:2731832/148‑1 (MQ=255)
                                                                                                    tttGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAg                                            <  2:2273227/148‑1 (MQ=255)
                                                                                                    tttGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAg                                            >  2:2418755/1‑148 (MQ=255)
                                                                                                       gAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGccc                                         <  1:2153083/148‑1 (MQ=255)
                                                                                                       gAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGccc                                         <  2:1292758/148‑1 (MQ=255)
                                                                                                       gAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGccc                                         <  2:3439639/148‑1 (MQ=255)
                                                                                                       gAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGccc                                         <  2:462183/148‑1 (MQ=255)
                                                                                                          aTCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGt                                      <  1:1347787/148‑1 (MQ=255)
                                                                                                          aTCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGt                                      >  2:2077060/1‑148 (MQ=255)
                                                                                                           tCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTc                                     >  1:1421023/1‑148 (MQ=255)
                                                                                                              tttGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGc                                  >  1:1354606/1‑148 (MQ=255)
                                                                                                               ttGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCa                                 >  1:2094859/1‑148 (MQ=255)
                                                                                                               ttGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACATTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGAAAAACAGCACGGCCCGCACAGCCCAGTCGGCa                                 >  1:2683207/1‑148 (MQ=255)
                                                                                                                tGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCaa                                <  2:1154223/148‑1 (MQ=255)
                                                                                                                    gAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACAt                            >  2:1818273/1‑148 (MQ=255)
                                                                                                                       ataatCTGCATTTTGTCGTTTATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTcc                         <  2:2738501/148‑1 (MQ=255)
                                                                                                                       ataatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTcc                         >  2:3721164/1‑148 (MQ=255)
                                                                                                                       ataatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTcc                         <  1:1517329/148‑1 (MQ=255)
                                                                                                                        taatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTccc                        <  1:1051198/148‑1 (MQ=255)
                                                                                                                        taatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTccc                        <  2:1080515/148‑1 (MQ=255)
                                                                                                                        taatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTccc                        <  1:552759/148‑1 (MQ=255)
                                                                                                                        taatCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTccc                        <  2:2989802/148‑1 (MQ=255)
                                                                                                                          atCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCAt                      >  1:771886/1‑148 (MQ=255)
                                                                                                                          atCAGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCAt                      >  1:2432744/1‑148 (MQ=255)
                                                                                                                           tCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATc                     <  2:2989494/148‑1 (MQ=255)
                                                                                                                           tCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATc                     >  1:1716002/1‑148 (MQ=255)
                                                                                                                           tCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATc                     >  1:3808643/1‑148 (MQ=255)
                                                                                                                              gCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAg                  >  1:1852871/1‑148 (MQ=255)
                                                                                                                              gCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCGCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAg                  >  1:1799146/1‑148 (MQ=255)
                                                                                                                                       cGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACGTTCCCATCCAGACaccaacc         >  2:797201/1‑148 (MQ=255)
                                                                                                                                          ttATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACcaca      >  2:1829302/1‑148 (MQ=255)
                                                                                                                                          ttATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACcaca      <  2:65426/148‑1 (MQ=255)
                                                                                                                                           tATTCCTACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCacac     >  1:2887831/1‑148 (MQ=255)
                                                                                                                                              tCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACAcccc  >  1:2919568/1‑148 (MQ=255)
                                                                                                                                              tCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACAcccc  >  1:3629566/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTTCACACTTTTGCGGCTCGGGTCGAGGGTATTTCCATAGCCAACGTCCAGTAACCATTCGCCAGTAAAACAGCACGGCCCGCACAGCCCAGTCGGCAAACATTCCCATCCAGACACCAACCACACCCC  >  CP009273/2051546‑2051835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑