Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,093,612 C→A 45.2% E380* (GAA→TAA)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,093,6120CA45.2% 186.6 / 276.1 232E380* (GAA→TAA) gnd6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base C (58/69);  new base A (46/59);  total (104/128)
Fisher's exact test for biased strand distribution p-value = 7.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

CGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAA  >  CP009273/2093465‑2093758
                                                                                                                                                   |                                                                                                                                                  
cGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTc                                                                                                                                                    <  1:1190863/148‑1 (MQ=255)
   aaTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGc                                                                                                                                                 <  1:2535517/148‑1 (MQ=255)
   aaTAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGc                                                                                                                                                 <  2:2225454/148‑1 (MQ=255)
      aGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACGTCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATa                                                                                                                                              >  2:3453484/1‑148 (MQ=255)
       ggCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATaa                                                                                                                                             <  2:2916627/148‑1 (MQ=255)
        gCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAg                                                                                                                                            <  1:1839300/148‑1 (MQ=255)
         cAACCGCTGCGGAGAAGGTCTGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGc                                                                                                                                           <  2:3679445/148‑1 (MQ=255)
         cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGc                                                                                                                                           <  2:2900741/148‑1 (MQ=255)
         cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGc                                                                                                                                           <  1:2005001/148‑1 (MQ=255)
         cAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGc                                                                                                                                           <  2:2259696/148‑1 (MQ=255)
           aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCAt                                                                                                                                         <  1:448550/148‑1 (MQ=255)
           aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCAt                                                                                                                                         >  2:3404723/1‑148 (MQ=255)
           aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCAt                                                                                                                                         >  2:1505321/1‑148 (MQ=255)
           aCCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCAt                                                                                                                                         <  2:3466353/148‑1 (MQ=255)
            ccGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATc                                                                                                                                        >  1:1070227/1‑148 (MQ=255)
             cGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCg                                                                                                                                       <  2:1988627/148‑1 (MQ=255)
               cTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGt                                                                                                                                     <  1:112957/148‑1 (MQ=255)
                 gCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCATTTTGCTTGAAGTACGGAGCCATCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGa                                                                                                                                   <  2:139511/148‑1 (MQ=255)
                 gCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGa                                                                                                                                   <  2:1880683/148‑1 (MQ=255)
                    gagaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGAttt                                                                                                                                >  2:3745970/1‑148 (MQ=255)
                     agaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGAtttt                                                                                                                               <  1:13306/148‑1 (MQ=255)
                     agaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGAtttt                                                                                                                               >  1:3394668/1‑148 (MQ=255)
                      gaAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTc                                                                                                                              >  2:1785873/1‑148 (MQ=255)
                         ggTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGc                                                                                                                           >  1:551949/1‑148 (MQ=255)
                         ggTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGc                                                                                                                           >  1:3027634/1‑148 (MQ=255)
                               aaCCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAAc                                                                                                                     >  2:393902/1‑148 (MQ=255)
                                    gAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTgcgc                                                                                                                >  2:2342313/1‑148 (MQ=255)
                                      aTACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCAc                                                                                                              >  2:3341709/1‑148 (MQ=255)
                                       tACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACg                                                                                                             >  2:1599364/1‑148 (MQ=255)
                                       tACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACg                                                                                                             >  2:2531763/1‑148 (MQ=255)
                                        tccGGTCTGTACTGCATAAGCAACGACATCCCGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACgg                                                                                                            <  2:2856647/147‑1 (MQ=255)
                                        aCCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGACCTGCGCACgg                                                                                                            >  1:279486/1‑148 (MQ=255)
                                        aCCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACgg                                                                                                            <  1:3703622/148‑1 (MQ=255)
                                        aCCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACgg                                                                                                            <  2:371729/148‑1 (MQ=255)
                                         ccGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAGCTGCGCACGga                                                                                                           <  1:166006/148‑1 (MQ=255)
                                         ccGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGga                                                                                                           <  1:1191898/148‑1 (MQ=255)
                                          cGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgat                                                                                                          <  2:788355/148‑1 (MQ=255)
                                           gTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatg                                                                                                         <  1:2621166/148‑1 (MQ=255)
                                           gTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatg                                                                                                         <  1:3469534/148‑1 (MQ=255)
                                           gTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatg                                                                                                         <  1:1570759/148‑1 (MQ=255)
                                           gTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatg                                                                                                         <  1:1693654/148‑1 (MQ=255)
                                             tCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGgatgat                                                                                                       >  2:2059101/1‑148 (MQ=255)
                                              cTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGatgatg                                                                                                      <  1:3215741/148‑1 (MQ=255)
                                               tGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGc                                                                                                     <  1:3731326/148‑1 (MQ=255)
                                                gTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGatgatgta                                                                                                    >  2:183918/1‑146 (MQ=255)
                                                  aCTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc                                                                                                  <  2:644956/148‑1 (MQ=255)
                                                  aCTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagc                                                                                                  <  2:2481115/148‑1 (MQ=255)
                                                     gCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagccag                                                                                               >  2:1016998/1‑148 (MQ=255)
                                                     gCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGcagccag                                                                                               >  2:456996/1‑148 (MQ=255)
                                                         aaGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACg                                                                                           >  1:1519277/1‑148 (MQ=255)
                                                              aCGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGaaaa                                                                                      <  2:1679192/148‑1 (MQ=255)
                                                               cGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAAt                                                                                     >  2:542786/1‑148 (MQ=255)
                                                                 aCATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCt                                                                                   <  2:3808526/148‑1 (MQ=255)
                                                                 aCATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCt                                                                                   >  2:825680/1‑148 (MQ=255)
                                                                 aCATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCt                                                                                   >  2:2959264/1‑148 (MQ=255)
                                                                  cATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCtt                                                                                  >  1:426392/1‑148 (MQ=255)
                                                                    tCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcg                                                                                >  1:2292436/1‑148 (MQ=255)
                                                                     cACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcgc                                                                               >  2:1851476/1‑148 (MQ=255)
                                                                      aCGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTcgcg                                                                              <  1:2448631/148‑1 (MQ=255)
                                                                       cGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGa                                                                             <  1:365653/148‑1 (MQ=255)
                                                                         cAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGAtt                                                                           <  1:1954479/148‑1 (MQ=255)
                                                                              ccTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGcc                                                                      >  2:1930858/1‑148 (MQ=255)
                                                                                 gctgGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTa                                                                   <  2:752492/148‑1 (MQ=255)
                                                                                    ggTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGtt                                                                <  1:1738586/148‑1 (MQ=255)
                                                                                    ggTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGtt                                                                <  1:36095/148‑1 (MQ=255)
                                                                                       aGTCATCGGCAATTTGCTTCAAGTACGGAGCCAGCACCAGGTTAGAGATCTGTGGATTTTCGGCATAAGCATCGGAGATTTTCTGCAGGACCAGCGCACGGATGATGTAGCCAGCAAGGAAAATCTTCGCGATTTCTCCGTAGTTCAg                                                             >  2:2260212/1‑148 (MQ=255)
                                                                                         tCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGAt                                                           >  2:2749661/1‑148 (MQ=255)
                                                                                         tCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGAt                                                           >  1:2343822/1‑148 (MQ=255)
                                                                                          cATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATc                                                          <  2:303621/148‑1 (MQ=255)
                                                                                          cATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATc                                                          >  2:3252170/1‑148 (MQ=255)
                                                                                           aTCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                         <  1:749158/148‑1 (MQ=255)
                                                                                           aTCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATcc                                                         <  2:551949/148‑1 (MQ=255)
                                                                                            tCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATccc                                                        >  2:1161693/1‑148 (MQ=255)
                                                                                               gCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGt                                                     >  2:1435916/1‑148 (MQ=255)
                                                                                               gCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGGGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGTAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGt                                                     >  2:3542691/1‑148 (MQ=255)
                                                                                                   tttGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTa                                                 <  1:1851476/148‑1 (MQ=255)
                                                                                                   tttGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTa                                                 >  1:499672/1‑148 (MQ=255)
                                                                                                      gCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTActc                                              <  2:2603683/148‑1 (MQ=255)
                                                                                                        ttGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCtt                                            <  1:951023/148‑1 (MQ=255)
                                                                                                          gAAGTACGGAGCCAGCAACAGGTTATCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGTTGCAGGCAGCACGGTTAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCa                                          <  1:3746754/148‑1 (MQ=255)
                                                                                                          gAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCa                                          <  1:1952768/148‑1 (MQ=255)
                                                                                                            aGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGa                                        <  1:3587424/148‑1 (MQ=255)
                                                                                                                       aGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgca                             >  1:848273/1‑148 (MQ=255)
                                                                                                                         cAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGacgcagcacgcagc                           >  1:3446587/1‑148 (MQ=255)
                                                                                                                           aCAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                         >  1:1916564/1‑148 (MQ=255)
                                                                                                                           aCAGGTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTg                         <  1:1704618/148‑1 (MQ=255)
                                                                                                                              ggTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgaga                      >  1:309276/1‑148 (MQ=255)
                                                                                                                               gTTAGCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTgagag                     >  1:615946/1‑148 (MQ=255)
                                                                                                                                   gCGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGc                 <  2:1519287/148‑1 (MQ=255)
                                                                                                                                    cGATCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGcc                >  1:2521261/1‑148 (MQ=255)
                                                                                                                                      aTCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCt              <  1:2927279/148‑1 (MQ=255)
                                                                                                                                       tCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGTAGCCCTg             <  1:2413919/148‑1 (MQ=255)
                                                                                                                                       tCTGTGGATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTg             >  1:3299708/1‑148 (MQ=255)
                                                                                                                                          gtgGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGc          >  1:1302046/1‑148 (MQ=255)
                                                                                                                                           tgGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCg         <  2:2377913/148‑1 (MQ=255)
                                                                                                                                             gATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGTAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTa       <  1:2358337/148‑1 (MQ=255)
                                                                                                                                             gATTTTAGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTa       <  1:2531719/148‑1 (MQ=255)
                                                                                                                                              aTTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTaa      >  1:1378919/1‑148 (MQ=255)
                                                                                                                                                tttCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGa    <  2:1822817/148‑1 (MQ=255)
                                                                                                                                                  tCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGaaa  <  2:343616/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTAATAGGCAACCGCTGCGGAGAAGGTCGGAACCGGAATACCGTTCTGTACTGCATAAGCAACGACATCACGCAGCGCCTGCTGGTAGTCATCGGCAATTTGCTTGAAGTACGGAGCCAGCAACAGGTTAGCGATCTGTGGATTTTCGGCATAAGCATCGGTGATTTTCTGCAGGAACTGCGCACGGATGATGCAGCCAGCACGGAAAATCTTCGCGATTTCGCCGTAGTTCAGATCCCAGTTGTACTCTTCAGACGCAGCACGCAGCTGAGAGAAGCCCTGGGCGTAAGAAA  >  CP009273/2093465‑2093758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑