Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,149,603 A→T 100% E287V (GAA→GTA)  mdtB → multidrug efflux system, subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,149,6030AT98.2% 770.7 / 2.0 237E287V (GAA→GTA) mdtBmultidrug efflux system, subunit B
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base T (136/96);  total (139/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 237 total aligned reads displayed.

TCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAA  >  CP009273/2149458‑2149747
                                                                                                                                                 |                                                                                                                                                
tCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTAc                                                                                                                                                <  2:3276046/148‑1 (MQ=255)
   gcgAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAgg                                                                                                                                             <  2:480756/148‑1 (MQ=255)
    cgAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGc                                                                                                                                            <  2:2056252/148‑1 (MQ=255)
       aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAt                                                                                                                                         >  1:3401542/1‑148 (MQ=255)
       aCGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAt                                                                                                                                         >  1:1299409/1‑148 (MQ=255)
        cGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                        >  1:1102675/1‑148 (MQ=255)
        cGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                        >  1:489205/1‑148 (MQ=255)
        cGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                        >  2:1626154/1‑148 (MQ=255)
        cGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGCGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCAtt                                                                                                                                        >  2:2803303/1‑148 (MQ=255)
         gACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtg                                                                                                                                       <  2:529798/148‑1 (MQ=255)
          aCCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgt                                                                                                                                      >  1:2042144/1‑148 (MQ=255)
           ccAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgtg                                                                                                                                     <  1:3076212/148‑1 (MQ=255)
           ccAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATtgtg                                                                                                                                     <  1:720965/148‑1 (MQ=255)
             aGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgat                                                                                                                                   <  1:1707045/148‑1 (MQ=255)
               aTGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatga                                                                                                                                 <  1:1874360/148‑1 (MQ=255)
               aTGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGtgatga                                                                                                                                 >  2:2527148/1‑148 (MQ=255)
                tGCAATCCGCCGAAGAGTATAGACAGCTAATCATCGCCTACCAGAACTGCGCGACCATTCGTCTGTGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGATGGCTCGGCGCTTGTGCGAACAAAGTACAGGCCATTGTGATGaa                                                                                                                                <  1:1730566/148‑1 (MQ=255)
                    aTCCTCCGAAGAGTATCGCCAGCTAATAATCGACTACCAGAACGGCGCGCCAATTCGTCTCTGCTATTTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTTTGATGAAtttt                                                                                                                            >  2:794650/1‑148 (MQ=255)
                    aTCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGtt                                                                                                                            >  1:1014503/1‑148 (MQ=255)
                     tCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTc                                                                                                                           >  1:262666/1‑148 (MQ=255)
                      ccgccgAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCa                                                                                                                          <  1:3087472/148‑1 (MQ=255)
                       cgccgTAGAGTATCGCCAGCTAATCCTCGCCTACCAGAAGGGCGAGGCTATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAg                                                                                                                         <  1:1688367/148‑1 (MQ=255)
                            aaGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCa                                                                                                                    >  1:3155761/1‑148 (MQ=255)
                             agagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAg                                                                                                                   >  2:1529908/1‑148 (MQ=255)
                             agagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAg                                                                                                                   >  1:587165/1‑148 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  >  1:862325/1‑148 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  >  1:1170055/1‑148 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  <  2:2350313/148‑1 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  >  2:980342/1‑148 (MQ=255)
                              gagTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGc                                                                                                                  >  2:698175/1‑148 (MQ=255)
                               agTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGcc                                                                                                                 >  1:790572/1‑148 (MQ=255)
                               agTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGcc                                                                                                                 >  2:3305990/1‑148 (MQ=255)
                               agTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGcc                                                                                                                 >  1:3567074/1‑148 (MQ=255)
                               agTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGcc                                                                                                                 >  1:2152779/1‑148 (MQ=255)
                                 tATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACCGGCCATGGTGATGAATGTTCAGCGCCAGCCCg                                                                                                               >  2:3165569/1‑148 (MQ=255)
                                 tATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCg                                                                                                               >  1:2731272/1‑148 (MQ=255)
                                  aTCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCgg                                                                                                              >  1:3627134/1‑148 (MQ=255)
                                  aTCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCgg                                                                                                              >  1:2220457/1‑148 (MQ=255)
                                        aGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTaa                                                                                                        <  1:3239128/148‑1 (MQ=255)
                                         gCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAc                                                                                                       >  1:461167/1‑148 (MQ=255)
                                         gCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAAc                                                                                                       >  1:1775979/1‑148 (MQ=255)
                                          cTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACa                                                                                                      >  2:2544625/1‑148 (MQ=255)
                                            aaTCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACAtt                                                                                                    >  1:3311713/1‑148 (MQ=255)
                                             atcatcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTa                                                                                                   >  2:3359262/1‑148 (MQ=255)
                                               catcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtc                                                                                                 >  1:2661420/1‑148 (MQ=255)
                                                atcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtct                                                                                                >  2:1101191/1‑148 (MQ=255)
                                                atcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtct                                                                                                <  1:521206/148‑1 (MQ=255)
                                                atcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtct                                                                                                <  1:2441610/148‑1 (MQ=255)
                                                 tcGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTAtctc                                                                                               >  1:927895/1‑148 (MQ=255)
                                                      tACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccg                                                                                          >  1:3297188/1‑148 (MQ=255)
                                                        ccAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgcc                                                                                        >  2:682380/1‑148 (MQ=255)
                                                         cAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCAccgccg                                                                                       >  2:2739118/1‑148 (MQ=255)
                                                           gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                     >  1:3349669/1‑148 (MQ=255)
                                                           gAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGAc                                                                                     >  1:3037834/1‑148 (MQ=255)
                                                            aaCGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACa                                                                                    >  1:2084940/1‑148 (MQ=255)
                                                                gcgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCAt                                                                                <  2:3484425/148‑1 (MQ=255)
                                                                gcgcgcCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCAt                                                                                <  2:1588380/148‑1 (MQ=255)
                                                                    gcCAAGTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCgg                                                                            <  2:1707827/148‑1 (MQ=255)
                                                                     ccAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGc                                                                           >  2:2886415/1‑148 (MQ=255)
                                                                     ccAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGc                                                                           >  1:1519243/1‑148 (MQ=255)
                                                                       aaTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAg                                                                         >  1:105108/1‑148 (MQ=255)
                                                                       aaTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAg                                                                         >  1:3374040/1‑148 (MQ=255)
                                                                        aTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                        >  2:1746515/1‑148 (MQ=255)
                                                                        aTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                        >  2:2226921/1‑148 (MQ=255)
                                                                        aTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                        >  2:2226352/1‑148 (MQ=255)
                                                                        aTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                        >  2:2063838/1‑148 (MQ=255)
                                                                        aTTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGa                                                                        >  2:88617/1‑148 (MQ=255)
                                                                         ttCGTCTGGGCGATGACGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAt                                                                       >  1:3789780/1‑148 (MQ=255)
                                                                          tCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtg                                                                      <  2:3057248/148‑1 (MQ=255)
                                                                          tCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtg                                                                      <  2:1219990/148‑1 (MQ=255)
                                                                           cGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgc                                                                     <  2:564820/148‑1 (MQ=255)
                                                                             tCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGAtgctg                                                                   <  1:2064612/148‑1 (MQ=255)
                                                                                gggCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCca                                                                <  2:1525007/148‑1 (MQ=255)
                                                                                   cGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAg                                                             >  1:2959667/1‑148 (MQ=255)
                                                                                       gTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCa                                                         <  2:1930893/148‑1 (MQ=255)
                                                                                           cAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTga                                                     <  2:3697630/148‑1 (MQ=255)
                                                                                              cTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGagag                                                  >  2:2143502/1‑148 (MQ=255)
                                                                                                  agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCCCCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCCCTGAGAGTCTg                                              >  2:2752644/1‑148 (MQ=255)
                                                                                                  agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTg                                              >  2:838233/1‑148 (MQ=255)
                                                                                                         ggTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAt                                       <  1:2373541/148‑1 (MQ=255)
                                                                                                         ggTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAAt                                       <  1:852914/148‑1 (MQ=255)
                                                                                                            gCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCgg                                    <  1:509825/148‑1 (MQ=255)
                                                                                                            gCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCgg                                    <  1:1453749/148‑1 (MQ=255)
                                                                                                              aGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTg                                  <  2:2638496/148‑1 (MQ=255)
                                                                                                               gAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATATCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                 >  2:111715/1‑148 (MQ=255)
                                                                                                                aaaaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGaa                                >  2:1910059/1‑148 (MQ=255)
                                                                                                                  aaCAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAgg                              >  2:1644907/1‑148 (MQ=255)
                                                                                                                    cAGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTg                            >  1:873286/1‑148 (MQ=255)
                                                                                                                     aGCTGGCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGa                           <  2:3375449/148‑1 (MQ=255)
                                                                                                                       cTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACa                         <  1:698175/148‑1 (MQ=255)
                                                                                                                          gCTCGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTg                      <  1:1219907/148‑1 (MQ=255)
                                                                                                                             cGGCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCtt                   <  1:3724790/148‑1 (MQ=255)
                                                                                                                              ggCGCGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCttt                  <  1:1423567/148‑1 (MQ=255)
                                                                                                                               gcgcGTGGGCGAACAAAGTACAGGCCATTGTGATGAATGCTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTc                 <  2:2091244/148‑1 (MQ=255)
                                                                                                                                cgcgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTcc                >  1:647307/1‑148 (MQ=255)
                                                                                                                                cgcgTGGGCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTcc                >  1:2911108/1‑148 (MQ=255)
                                                                                                                                      ggCGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGc          >  1:343143/1‑148 (MQ=255)
                                                                                                                                        cGAACAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcac        <  2:3253352/148‑1 (MQ=255)
                                                                                                                                          aaCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcacca      >  2:649791/1‑148 (MQ=255)
                                                                                                                                           aCAAAGTACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcaccac     >  1:2310468/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCGCGAACGACCAGATGCAATCCGCCGAAGAGTATCGCCAGCTAATCATCGCCTACCAGAACGGCGCGCCAATTCGTCTGGGCGATGTCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAA  >  CP009273/2149458‑2149747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑