Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,218,022 C→A 100% E110* (GAA→TAA)  pbpG ← D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,218,0220CA99.1% 733.7 / ‑1.8 223E110* (GAA→TAA) pbpGD‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (110/111);  total (112/111)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

TTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCAC  >  CP009273/2217877‑2218166
                                                                                                                                                 |                                                                                                                                                
ttgttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGc                                                                                                                                                >  1:1211127/1‑148 (MQ=255)
  gttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTa                                                                                                                                              >  2:3797487/1‑148 (MQ=255)
   ttCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTAt                                                                                                                                             >  2:3789435/1‑148 (MQ=255)
       ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                         >  2:2155239/1‑148 (MQ=255)
       ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                         <  1:508631/148‑1 (MQ=255)
       ttCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAg                                                                                                                                         <  1:1370745/148‑1 (MQ=255)
        tCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGt                                                                                                                                        <  2:1786099/148‑1 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  2:1104872/1‑148 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  2:3540872/1‑148 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  1:2714684/1‑148 (MQ=255)
                 tttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgc                                                                                                                               >  1:2006664/1‑148 (MQ=255)
                  ttcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTAcgcgcg                                                                                                                              >  1:2411043/1‑148 (MQ=255)
                   tcgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGa                                                                                                                             <  1:2086185/148‑1 (MQ=255)
                    cgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGaa                                                                                                                            <  1:2490067/148‑1 (MQ=255)
                    cgctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGaa                                                                                                                            <  1:312442/148‑1 (MQ=255)
                     gctttcgcATTCATTGCCTTAATAACGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAAt                                                                                                                           <  1:2283562/148‑1 (MQ=255)
                     gctttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAAt                                                                                                                           <  1:356944/148‑1 (MQ=255)
                       tttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAg                                                                                                                         <  2:922748/148‑1 (MQ=255)
                       tttcgcATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAg                                                                                                                         <  1:1620279/148‑1 (MQ=255)
                             attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                   >  1:2563270/1‑148 (MQ=255)
                             attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                   <  2:2885378/148‑1 (MQ=255)
                             attcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCt                                                                                                                   <  2:1007105/148‑1 (MQ=255)
                              ttcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCtt                                                                                                                  <  2:1456988/148‑1 (MQ=255)
                               tcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTGTTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                 <  1:2364349/148‑1 (MQ=255)
                               tcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                 >  1:3773546/1‑148 (MQ=255)
                               tcattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCttt                                                                                                                 >  2:2372720/1‑148 (MQ=255)
                                cattGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTc                                                                                                                <  1:3520082/148‑1 (MQ=255)
                                 attGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCa                                                                                                               <  2:129500/148‑1 (MQ=255)
                                  ttGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAt                                                                                                              >  1:116937/1‑148 (MQ=255)
                                  ttGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAt                                                                                                              <  2:1497213/148‑1 (MQ=255)
                                   tGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtc                                                                                                             >  2:354683/1‑148 (MQ=255)
                                     ccTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtctc                                                                                                           <  1:2787873/148‑1 (MQ=255)
                                     ccTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCAtctc                                                                                                           <  1:2787393/148‑1 (MQ=255)
                                           taaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGt                                                                                                     <  2:444766/148‑1 (MQ=255)
                                           taaAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGt                                                                                                     >  1:502929/1‑148 (MQ=255)
                                               ggCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                 <  1:897802/148‑1 (MQ=255)
                                                gCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                 >  1:3680404/1‑147 (MQ=255)
                                                gCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTgg                                                                                                 <  2:3680404/147‑1 (MQ=255)
                                                gCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGc                                                                                                <  1:420744/148‑1 (MQ=255)
                                                 catagTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCt                                                                                               >  2:3434192/5‑148 (MQ=255)
                                                 cTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCt                                                                                               <  2:1625023/148‑1 (MQ=255)
                                                 cTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCt                                                                                               >  2:3433684/1‑148 (MQ=255)
                                                  tttGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTg                                                                                              <  2:1351999/148‑1 (MQ=255)
                                                     gTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGata                                                                                           <  2:1148997/148‑1 (MQ=255)
                                                      tAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGatat                                                                                          <  2:1364568/148‑1 (MQ=255)
                                                       aaCCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATc                                                                                         >  2:2451402/1‑148 (MQ=255)
                                                         ccaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCa                                                                                       >  2:600179/1‑148 (MQ=255)
                                                         ccaccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCa                                                                                       >  2:1536775/1‑148 (MQ=255)
                                                            ccGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACtt                                                                                    >  1:1124066/1‑148 (MQ=255)
                                                                gATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAg                                                                                >  1:3423776/1‑148 (MQ=255)
                                                                gATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAg                                                                                <  2:63859/148‑1 (MQ=255)
                                                                 aTAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGt                                                                               <  1:1656400/148‑1 (MQ=255)
                                                                  tAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGtt                                                                              >  2:1195003/1‑148 (MQ=255)
                                                                     tGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGttttt                                                                           >  1:3249755/1‑148 (MQ=255)
                                                                      ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTc                                                                          >  1:1064960/1‑148 (MQ=255)
                                                                      ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTc                                                                          <  2:3075652/148‑1 (MQ=255)
                                                                      ggTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTc                                                                          <  2:3199074/148‑1 (MQ=255)
                                                                         gcgcAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATc                                                                       <  1:2060688/148‑1 (MQ=255)
                                                                          cgcAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATcc                                                                      <  2:3692672/148‑1 (MQ=255)
                                                                             aaGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGc                                                                   >  2:159588/1‑148 (MQ=255)
                                                                               ggCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCACCCAGCgg                                                                 <  2:1210538/148‑1 (MQ=255)
                                                                                gCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGc                                                                >  2:1754446/1‑148 (MQ=255)
                                                                                          gcgcgGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCAt                                                      >  2:2499500/1‑148 (MQ=255)
                                                                                                ttttCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGca                                                >  2:84675/1‑148 (MQ=255)
                                                                                                 tttCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGcac                                               >  2:1290310/1‑148 (MQ=255)
                                                                                                 tttCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGcac                                               >  2:262850/1‑148 (MQ=255)
                                                                                                   tCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACaa                                             <  1:2155239/148‑1 (MQ=255)
                                                                                                          aCATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATcgc                                      >  2:2815236/1‑148 (MQ=255)
                                                                                                            aTCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCgg                                    <  1:2882446/148‑1 (MQ=255)
                                                                                                            aTCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCgg                                    >  1:2241919/1‑148 (MQ=255)
                                                                                                               aGCGCCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCa                                 <  1:2143034/148‑1 (MQ=255)
                                                                                                                gcgcCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCAt                                <  2:858091/148‑1 (MQ=255)
                                                                                                                gcgcCAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCAt                                <  2:2381270/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTaa                             <  2:2563270/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTaa                             <  1:1741012/148‑1 (MQ=255)
                                                                                                                   ccAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTaa                             <  2:765731/148‑1 (MQ=255)
                                                                                                                    cAGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAt                            <  2:2812366/148‑1 (MQ=255)
                                                                                                                     aGCAACATCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAtt                           <  2:2048196/148‑1 (MQ=255)
                                                                                                                     aGCAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAAtt                           <  1:413204/148‑1 (MQ=255)
                                                                                                                       cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTg                         >  1:641707/1‑148 (MQ=255)
                                                                                                                       cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTg                         <  1:1956579/148‑1 (MQ=255)
                                                                                                                       cAACAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTg                         <  1:1957497/148‑1 (MQ=255)
                                                                                                                        aaCAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                        <  1:2431641/148‑1 (MQ=255)
                                                                                                                        aaCAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGc                        <  2:3616664/148‑1 (MQ=255)
                                                                                                                          cAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTg                      >  1:1351156/1‑148 (MQ=255)
                                                                                                                          cAGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTg                      >  2:2365108/1‑148 (MQ=255)
                                                                                                                           aGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGa                     >  1:2325823/1‑148 (MQ=255)
                                                                                                                           aGCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTGTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGa                     >  2:780283/1‑148 (MQ=255)
                                                                                                                            gCATATCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGAt                    >  2:305417/1‑148 (MQ=255)
                                                                                                                               tatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                 >  1:2966101/1‑148 (MQ=255)
                                                                                                                               tatCTTTACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                 <  1:729323/148‑1 (MQ=255)
                                                                                                                               tatCTTTACGGCTGATATAGCTATTCAGTCGTACTCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCAACCAGCACAACCATCGCGGTCATTAATTTGCTGATAGa                 >  2:677087/1‑148 (MQ=255)
                                                                                                                                   tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCa             <  2:502454/148‑1 (MQ=255)
                                                                                                                                   tttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCa             <  2:2428963/148‑1 (MQ=255)
                                                                                                                                    ttACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCaa            <  1:3797487/148‑1 (MQ=255)
                                                                                                                                    ttACGGCTGAGTTAGCTATTCACTCGTACGCGCGAATAGACCCCGTTCATCTCCGGCGTCTGGCTGATATCCACTTTTCGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCGGATAGACGCaa            <  2:2103089/148‑1 (MQ=255)
                                                                                                                                     tACGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAAt           <  1:537722/148‑1 (MQ=255)
                                                                                                                                      aCGGCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATc          <  2:988836/148‑1 (MQ=255)
                                                                                                                                        ggCTGATTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCgg        <  1:3789435/148‑1 (MQ=255)
                                                                                                                                             aTTTAGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCa   <  2:1392176/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCGCTATTCAGTCGTACGCGCGAATAGACCCCTTTCATCTCCGGCGTCTGGCTGATATCCACTTTTAGTTTTTCATCCAGCGGCAGTCGTGCATCCAGCACAACCATCGCGGTCATTAATTTGCTGATAGACGCAATCGGACGCAC  >  CP009273/2217877‑2218166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑