Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,248,657 C→A 100% K3N (AAG→AAT)  rihB ← ribonucleoside hydrolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,248,6570CA98.7% 780.9 / ‑0.9 253K3N (AAG→AAT) rihBribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (125/122);  total (125/128)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

TCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAG  >  CP009273/2248512‑2248801
                                                                                                                                                 |                                                                                                                                                
tCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGAGGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGTCCCGGATCACAATCCAGAATAATTTTTCTAtt                                                                                                                                                >  2:3417588/1‑148 (MQ=255)
  aGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTAtttt                                                                                                                                              <  1:2000079/148‑1 (MQ=255)
   ggCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTc                                                                                                                                             <  1:3029182/148‑1 (MQ=255)
    gCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTcc                                                                                                                                            <  2:1605186/148‑1 (MQ=255)
       attaattaATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCAtt                                                                                                                                         <  1:3313961/148‑1 (MQ=255)
       attaattaATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCAtt                                                                                                                                         >  1:3234575/1‑148 (MQ=255)
        ttaattaaTGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTg                                                                                                                                        <  2:2475716/148‑1 (MQ=255)
         taattaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGt                                                                                                                                       >  2:849005/1‑148 (MQ=255)
           attaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGttt                                                                                                                                     >  2:1940358/1‑148 (MQ=255)
            ttaatGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTa                                                                                                                                    <  1:3346442/148‑1 (MQ=255)
                tGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                                <  1:3758455/148‑1 (MQ=255)
                tGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                                <  1:3653892/148‑1 (MQ=255)
                tGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTc                                                                                                                                >  2:2599658/1‑148 (MQ=255)
                  ttttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCct                                                                                                                              <  1:354781/148‑1 (MQ=255)
                   tttATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCctc                                                                                                                             >  2:3373590/1‑148 (MQ=255)
                       tCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGtt                                                                                                                         >  1:540773/1‑148 (MQ=255)
                        cAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGttt                                                                                                                        >  2:2876601/1‑148 (MQ=255)
                         aaGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTc                                                                                                                       >  2:1467693/1‑148 (MQ=255)
                         aaGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTc                                                                                                                       <  1:1390534/148‑1 (MQ=255)
                         aaGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTc                                                                                                                       <  2:181021/148‑1 (MQ=255)
                           gCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCa                                                                                                                     >  2:342165/1‑148 (MQ=255)
                            cGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAg                                                                                                                    <  1:3090921/148‑1 (MQ=255)
                             gTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCCTGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGt                                                                                                                   <  2:2387669/148‑1 (MQ=255)
                             gTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGt                                                                                                                   <  1:1488202/148‑1 (MQ=255)
                             gTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGt                                                                                                                   <  2:318582/148‑1 (MQ=255)
                                 gATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCg                                                                                                               >  2:2104476/1‑148 (MQ=255)
                                 gATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCg                                                                                                               >  2:2379942/1‑148 (MQ=255)
                                  aTTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGt                                                                                                              >  2:3659749/1‑148 (MQ=255)
                                  aTTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGt                                                                                                              >  1:2369700/1‑148 (MQ=255)
                                   ttACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGtt                                                                                                             >  1:1840640/1‑148 (MQ=255)
                                     aCCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAt                                                                                                           <  1:1132593/148‑1 (MQ=255)
                                        cGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTAtttt                                                                                                        <  2:628900/148‑1 (MQ=255)
                                              aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCNGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTAc                                                                                                  >  1:1938153/1‑148 (MQ=255)
                                              aaTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTAc                                                                                                  <  1:96257/148‑1 (MQ=255)
                                               aTAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACa                                                                                                 >  2:1752863/1‑148 (MQ=255)
                                                tAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAg                                                                                                >  1:1989269/1‑148 (MQ=255)
                                                   tGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCaa                                                                                             >  1:213219/1‑148 (MQ=255)
                                                     aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAg                                                                                           >  1:1834764/1‑148 (MQ=255)
                                                     aTGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAg                                                                                           >  1:3804055/1‑148 (MQ=255)
                                                        ccTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaaa                                                                                        <  2:3111191/148‑1 (MQ=255)
                                                        ccTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaaa                                                                                        <  1:2599658/148‑1 (MQ=255)
                                                        ccTAATAAATCTAGTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGaaa                                                                                        <  2:1205834/148‑1 (MQ=255)
                                                            ataaATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTc                                                                                    >  2:3301994/1‑148 (MQ=255)
                                                                aTCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGAcc                                                                                >  1:628172/1‑148 (MQ=255)
                                                                 tCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCTTTGTTTTTTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCg                                                                               <  2:1045724/148‑1 (MQ=255)
                                                                 tCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCg                                                                               >  2:30895/1‑148 (MQ=255)
                                                                 tCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCg                                                                               >  1:2302098/1‑148 (MQ=255)
                                                                  cTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTATCTACAGCAAAGAAAATTCGACCgg                                                                              >  2:200731/1‑148 (MQ=255)
                                                                          ggATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatga                                                                      <  2:1512266/148‑1 (MQ=255)
                                                                           gATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCgatgat                                                                     >  2:2817744/1‑148 (MQ=255)
                                                                            aTGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGAtt                                                                    >  1:869493/1‑148 (MQ=255)
                                                                               tttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGaa                                                                 <  1:849005/148‑1 (MQ=255)
                                                                                ttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAAt                                                                >  2:2290618/1‑148 (MQ=255)
                                                                                ttCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAAt                                                                >  1:1279281/1‑148 (MQ=255)
                                                                                  cgccgcCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCt                                                              <  1:2876601/148‑1 (MQ=255)
                                                                                      gccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaac                                                          >  1:2047288/1‑148 (MQ=255)
                                                                                       ccATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaaca                                                         <  1:540420/148‑1 (MQ=255)
                                                                                        catcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaacaa                                                        <  2:2795139/148‑1 (MQ=255)
                                                                                        catcatTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTaacaa                                                        >  2:3425392/1‑148 (MQ=255)
                                                                                             ttATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcg                                                   >  2:208220/1‑148 (MQ=255)
                                                                                              tataGCAATAGCATCATCATGACCCGGATCACAATCCATAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCTTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgt                                                  <  1:74295/148‑1 (MQ=255)
                                                                                              tataGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgt                                                  <  2:3139265/148‑1 (MQ=255)
                                                                                                   caatagcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTACATTGTTTATTTAATCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGcgtacgta                                             >  1:3745910/1‑148 (MQ=255)
                                                                                                        gcaTCATCATGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTa                                        >  2:2342464/1‑148 (MQ=255)
                                                                                                         catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                       >  2:1431920/1‑148 (MQ=255)
                                                                                                         catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                       >  1:1026057/1‑148 (MQ=255)
                                                                                                         catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                       >  2:1977357/1‑148 (MQ=255)
                                                                                                         catcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaa                                       >  1:2245803/1‑148 (MQ=255)
                                                                                                          atcatcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTaaa                                      <  2:455323/148‑1 (MQ=255)
                                                                                                            catcatGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAata                                    <  1:424322/148‑1 (MQ=255)
                                                                                                            catcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAata                                    >  1:946739/1‑148 (MQ=255)
                                                                                                              tcatGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATg                                  <  2:1556956/148‑1 (MQ=255)
                                                                                                                atGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgag                                >  2:1177251/1‑148 (MQ=255)
                                                                                                                 tGACCCGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgaga                               >  1:1214822/1‑148 (MQ=255)
                                                                                                                 tGACCCGGATCACAATCCAGAATAATTTTTATATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATgaga                               <  1:2428685/148‑1 (MQ=255)
                                                                                                                    cccGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAt                            <  2:540812/148‑1 (MQ=255)
                                                                                                                    cccGGATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAt                            <  2:540753/148‑1 (MQ=255)
                                                                                                                       ggATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCTTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtctc                         <  2:768772/148‑1 (MQ=255)
                                                                                                                       ggATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtctc                         <  2:766502/148‑1 (MQ=255)
                                                                                                                       ggATCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAAtctc                         <  1:3417588/148‑1 (MQ=255)
                                                                                                                         aTCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCAt                       <  1:1601954/148‑1 (MQ=255)
                                                                                                                          tCACAATCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAAAAACAGCGTACGTATGCAAAATATGAGAAATCTCATa                      >  1:725160/1‑148 (MQ=255)
                                                                                                                           cacaATCCAGAATAATTTTTATATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAg                     <  2:437988/148‑1 (MQ=255)
                                                                                                                             caatcaatAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCg                   <  2:3533445/148‑1 (MQ=255)
                                                                                                                               aTCCAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGa                 >  1:610150/1‑148 (MQ=255)
                                                                                                                                tcaaGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGAt                <  1:1703583/145‑1 (MQ=255)
                                                                                                                                 ccAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTAAGCTAAATATGAGAAATCTCATAGCGGATa               >  2:2681785/1‑148 (MQ=255)
                                                                                                                                  cataataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaa              <  2:1388946/145‑1 (MQ=255)
                                                                                                                                  cAGAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATaa              <  2:3436274/148‑1 (MQ=255)
                                                                                                                                    gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAAc            >  1:2339381/1‑148 (MQ=255)
                                                                                                                                    gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAAc            >  2:582505/1‑148 (MQ=255)
                                                                                                                                    gAATAATTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAAc            >  2:1863504/1‑148 (MQ=255)
                                                                                                                                     aataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACa           <  2:2460643/148‑1 (MQ=255)
                                                                                                                                      ataatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACAt          <  1:2817744/148‑1 (MQ=255)
                                                                                                                                        aatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGATAAATCTCATATCGGATAAACATCg        >  1:1068187/1‑148 (MQ=255)
                                                                                                                                        aatTTTTCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCg        <  2:38029/148‑1 (MQ=255)
                                                                                                                                           ttttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTga     >  2:1655707/1‑148 (MQ=255)
                                                                                                                                            tttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaa    <  1:2191831/148‑1 (MQ=255)
                                                                                                                                             ttCTATTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaa   <  1:1195964/148‑1 (MQ=255)
                                                                                                                                              tctaTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTgaaag  >  1:3154438/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTACCCGCTACAATAGTGATGCCTAATAAATCTATTGCCGGATGTTTCGCCGCCATCATTATAGCAATAGCATCATCATGACCCGGATCACAATCCAGAATAATTTTTCTCTTTTCCATTGTTTATTTCCTCTGTTTCCAGTTGCGTTATTTTTTCTACAGCAAAGAAAATTCGACCGGGCCGATGATTGAATCTTAACAACAGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAG  >  CP009273/2248512‑2248801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑