BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,277,743	A→T	19.0%	N46Y (AAC→TAC)	yejL →	UPF0352 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,277,743	0	A	T	19.0%	605.3 / 132.7	247	N46Y (AAC→TAC)	yejL	UPF0352 family protein
Reads supporting (aligned to +/- strand): ref base A (97/103); new base T (27/20); total (124/123)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 248 total aligned reads displayed.

TGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGT  >  CP009273/2277596‑2277889

   tttCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAaca                                                                                                                                                 >  2:3508296/1‑148 (MQ=255)

   tgtCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTaca                                                                                                                                                 <  2:1709192/146‑1 (MQ=255)

     tCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTATCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACAcc                                                                                                                                               >  1:507561/1‑148 (MQ=255)

      cGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCa                                                                                                                                              >  1:1226234/1‑148 (MQ=255)

        aTTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGc                                                                                                                                            >  1:2313314/1‑148 (MQ=255)

        aTTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGc                                                                                                                                            <  1:3242348/148‑1 (MQ=255)

          ttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCAt                                                                                                                                          >  2:2536928/1‑148 (MQ=255)

          ttATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCAt                                                                                                                                          >  2:2168095/1‑148 (MQ=255)

            aTGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTg                                                                                                                                        <  1:658650/148‑1 (MQ=255)

               ccACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGccc                                                                                                                                     <  2:3151996/148‑1 (MQ=255)

                cacaAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCATAGCTGATCAACGTACTTGAAAAACATAAGGATCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGTTCACTAACCTTATCAACACCAGCATTGaccc                                                                                                                                    >  1:62458/1‑148 (MQ=255)

                 acaAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCg                                                                                                                                   >  2:2299459/1‑148 (MQ=255)

                 acaAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCg                                                                                                                                   >  2:3122430/1‑148 (MQ=255)

                  caAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCgg                                                                                                                                  >  1:1742783/1‑148 (MQ=255)

                   aaaTTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGc                                                                                                                                 >  2:1406597/1‑148 (MQ=255)

                      tttCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCa                                                                                                                              >  1:1947129/1‑148 (MQ=255)

                      tttCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCa                                                                                                                              >  2:970145/1‑148 (MQ=255)

                          ccGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGc                                                                                                                          >  2:1233787/1‑148 (MQ=255)

                          ccGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGc                                                                                                                          >  2:2846644/1‑148 (MQ=255)

                              tACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAgg                                                                                                                      >  2:512222/1‑148 (MQ=255)

                                         aCAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAAc                                                                                                           >  1:2375425/1‑148 (MQ=255)

                                          cAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAAct                                                                                                          >  1:847028/1‑148 (MQ=255)

                                          cAGGTTGAACAATTGCTCGCAGAGCTGCACAACGTACTGGAAAAACATAAGGCTCCGAACGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAAct                                                                                                          >  2:2045238/1‑148 (MQ=255)

                                             gTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtt                                                                                                       <  2:682843/148‑1 (MQ=255)

                                             gTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtt                                                                                                       <  1:1004903/148‑1 (MQ=255)

                                             gTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtt                                                                                                       <  1:1004755/148‑1 (MQ=255)

                                             gTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtt                                                                                                       <  1:2051012/148‑1 (MQ=255)

                                              ttGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCttt                                                                                                      <  2:919312/148‑1 (MQ=255)

                                               tGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCtttt                                                                                                     >  2:3372407/1‑148 (MQ=255)

                                                gAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTg                                                                                                    <  2:456440/148‑1 (MQ=255)

                                                gAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTg                                                                                                    <  2:456362/148‑1 (MQ=255)

                                                      ttGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCcgcg                                                                                              >  2:3064325/1‑148 (MQ=255)

                                                            gCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTAc                                                                                        <  2:776680/148‑1 (MQ=255)

                                                               gagCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGt                                                                                     <  2:1345716/148‑1 (MQ=255)

                                                                agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    <  1:3103279/148‑1 (MQ=255)

                                                                agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    >  2:3817745/1‑148 (MQ=255)

                                                                agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    <  2:2631862/148‑1 (MQ=255)

                                                                agCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTc                                                                                    <  1:2471246/148‑1 (MQ=255)

                                                                 gctgctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTcc                                                                                   <  1:1977178/148‑1 (MQ=255)

                                                                  ctgctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCct                                                                                  >  2:825892/1‑148 (MQ=255)

                                                                  ctgctCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCct                                                                                  <  1:547658/148‑1 (MQ=255)

                                                                         aCGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCaa                                                                           <  2:324945/148‑1 (MQ=255)

                                                                         aCGTACTGGAAAAAAATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTTCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATcca                                                                           >  2:3734192/1‑146 (MQ=255)

                                                                          cGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAAc                                                                          >  2:1122348/1‑148 (MQ=255)

                                                                             aCTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGACCGCGCCTTACAGTCCTCTATCAACGaa                                                                       >  1:2273782/1‑148 (MQ=255)

                                                                                  aaaaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaa                                                                  <  2:3626092/148‑1 (MQ=255)

                                                                                  aaaaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaa                                                                  >  2:2346395/1‑148 (MQ=255)

                                                                                  aaaaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaa                                                                  <  2:2667652/148‑1 (MQ=255)

                                                                                  aaaaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACaa                                                                  <  1:3355102/148‑1 (MQ=255)

                                                                                    aaaCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAg                                                                      <  2:351045/142‑1 (MQ=255)

                                                                                      aCATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAgcg                                                              <  1:3765232/148‑1 (MQ=255)

                                                                                       cATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCTGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAgcgc                                                             <  2:525398/148‑1 (MQ=255)

                                                                                       cATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCTGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAgcgc                                                             <  2:2333318/148‑1 (MQ=255)

                                                                                       cATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCCGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAgcgc                                                             >  1:2557958/1‑148 (MQ=255)

                                                                                        aTAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCa                                                            <  2:1946491/148‑1 (MQ=255)

                                                                                        aTAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCa                                                            <  1:3122430/148‑1 (MQ=255)

                                                                                            ggCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTaa                                                        >  2:3604923/1‑148 (MQ=255)

                                                                                            ggCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTaa                                                        >  2:3236707/1‑148 (MQ=255)

                                                                                             gCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAg                                                       >  1:2308697/1‑148 (MQ=255)

                                                                                              cTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAgg                                                      >  1:306555/1‑148 (MQ=255)

                                                                                                ccgaccgaCCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGa                                                    <  1:1785581/148‑1 (MQ=255)

                                                                                                ccgaccgaCCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGa                                                    <  2:1649887/148‑1 (MQ=255)

                                                                                                 cgaccgacCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGaa                                                   <  1:2058169/148‑1 (MQ=255)

                                                                                                   accgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAAc                                                 >  2:218573/1‑148 (MQ=255)

                                                                                                     cgaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAg                                               <  2:3388366/148‑1 (MQ=255)

                                                                                                      gaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                              <  2:2483440/148‑1 (MQ=255)

                                                                                                      gaccTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGa                                              <  2:1567408/148‑1 (MQ=255)

                                                                                                         cTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                           >  1:2710213/1‑148 (MQ=255)

                                                                                                         cTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                           >  1:793257/1‑148 (MQ=255)

                                                                                                         cTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                           >  1:345548/1‑148 (MQ=255)

                                                                                                         cTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATaa                                           >  2:305526/1‑148 (MQ=255)

                                                                                                          tttCCCTGAGGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAAc                                          <  2:2451632/148‑1 (MQ=255)

                                                                                                           ttCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACa                                         >  2:1837945/1‑148 (MQ=255)

                                                                                                            tCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                        <  1:1695716/148‑1 (MQ=255)

                                                                                                            tCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAg                                        <  1:73174/148‑1 (MQ=255)

                                                                                                                tGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTa                                    >  2:2139311/1‑148 (MQ=255)

                                                                                                                tGATGGTGTTAGGCAACATGGTCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTa                                    <  1:403371/148‑1 (MQ=255)

                                                                                                                tGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTa                                    >  2:2100234/1‑148 (MQ=255)

                                                                                                                  aTGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATg                                  >  1:1279621/1‑148 (MQ=255)

                                                                                                                    ggTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCTACGCCAGGCATTTGCCTACTCTTTTGCCCGCGCCTTACAGTACTCTATCAACGAATACAAAGCGCACTACGGGAAACAGATAACAGGTTATGGt                                <  1:2168789/148‑1 (MQ=255)

                                                                                                                    ggTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGt                                >  2:3296903/1‑148 (MQ=255)

                                                                                                                     gtgtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTa                               <  1:360123/148‑1 (MQ=255)

                                                                                                                     gtgtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTa                               <  2:24262/148‑1 (MQ=255)

                                                                                                                       gtTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAAc                             >  1:2857098/1‑148 (MQ=255)

                                                                                                                         tAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTc                           <  1:2536928/148‑1 (MQ=255)

                                                                                                                          aGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCa                          <  2:2551330/148‑1 (MQ=255)

                                                                                                                          aGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCa                          <  1:14043/148‑1 (MQ=255)

                                                                                                                           ggCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCAt                         <  2:619323/148‑1 (MQ=255)

                                                                                                                           ggCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCAt                         <  2:619359/148‑1 (MQ=255)

                                                                                                                               aCATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTc                     <  2:3402122/148‑1 (MQ=255)

                                                                                                                                    gTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcgc                <  1:1110107/148‑1 (MQ=255)

                                                                                                                                    gTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAgcgc                >  1:1544080/1‑148 (MQ=255)

                                                                                                                                     tCACTAACCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCt               >  2:1486432/1‑148 (MQ=255)

                                                                                                                                      cACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTTAGCGCTa              <  1:970145/148‑1 (MQ=255)

                                                                                                                                        cTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTAcc            >  2:70698/1‑148 (MQ=255)

                                                                                                                                          aaCCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGt          >  1:605979/1‑148 (MQ=255)

                                                                                                                                           aCCTTATCTACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTg         <  1:1643294/148‑1 (MQ=255)

                                                                                                                                           aCCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTg         >  1:925802/1‑148 (MQ=255)

                                                                                                                                               tATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGaaaa     >  2:3247951/1‑148 (MQ=255)

                                                                                                                                                  cAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGt  <  1:3508637/148‑1 (MQ=255)

TGATTTCGATTTATGCCACAAATTTCCCGCTACAGTGATGAACAGGTTGAACAATTGCTCGCAGAGCTGCTCAACGTACTGGAAAAACATAAGGCTCCGACCGACCTTTCCCTGATGGTGTTAGGCAACATGGTCACTAACCTTATCAACACCAGCATTGCCCCGGCCCAACGCCAGGCAATTGCCAACTCTTTTGCCCGCGCCTTACAGTCCTCTATCAACGAAGACAAAGCGCACTAAGGGAAACAGATAACAGGTTATGGTAACTCATCGTCAGCGCTACCGTGAAAAAGT  >  CP009273/2277596‑2277889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`