Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,348,894 T→A 100% V384E (GTG→GAG)  glpB → sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,348,8940TA99.5% 674.1 / ‑4.6 203V384E (GTG→GAG) glpBsn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (112/90);  total (112/91)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCA  >  CP009273/2348749‑2349036
                                                                                                                                                 |                                                                                                                                              
cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGc                                                                                                                                              <  2:1756899/148‑1 (MQ=255)
cTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGc                                                                                                                                              <  1:2705349/148‑1 (MQ=255)
 tACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCt                                                                                                                                             <  1:2715696/148‑1 (MQ=255)
      aCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCg                                                                                                                                        >  2:79512/1‑148 (MQ=255)
      aCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCg                                                                                                                                        >  2:3335350/1‑148 (MQ=255)
      aCCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCg                                                                                                                                        >  1:1431175/1‑148 (MQ=255)
          ccACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGAtt                                                                                                                                    <  1:3778079/148‑1 (MQ=255)
              gcgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAt                                                                                                                                <  1:113788/148‑1 (MQ=255)
              gcgGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGAt                                                                                                                                <  2:3416659/148‑1 (MQ=255)
                ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                              <  1:698561/148‑1 (MQ=255)
                ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                              <  1:2449931/148‑1 (MQ=255)
                ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                              <  1:1810378/148‑1 (MQ=255)
                ggggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATcc                                                                                                                              <  1:141330/148‑1 (MQ=255)
                  ggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCa                                                                                                                            >  1:2385591/1‑148 (MQ=255)
                  ggTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCa                                                                                                                            <  2:54171/148‑1 (MQ=255)
                   gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCAt                                                                                                                           <  1:857902/148‑1 (MQ=255)
                   gTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCAt                                                                                                                           <  1:2820492/148‑1 (MQ=255)
                           tataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAgg                                                                                                                   >  1:3222635/1‑148 (MQ=255)
                           tataAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAgg                                                                                                                   >  1:1574685/1‑148 (MQ=255)
                           tataAGGGAGATTTTTTTGAGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAgg                                                                                                                   >  1:502923/1‑148 (MQ=255)
                             taAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGa                                                                                                                 >  2:1474083/1‑148 (MQ=255)
                             taAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGa                                                                                                                 <  2:2160199/148‑1 (MQ=255)
                              aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                >  2:2527841/1‑148 (MQ=255)
                              aaGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGAt                                                                                                                >  2:2966394/1‑148 (MQ=255)
                               aGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATg                                                                                                               >  2:446941/1‑148 (MQ=255)
                                   agaTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATgcggc                                                                                                           >  2:2228352/1‑148 (MQ=255)
                                     aTTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAACTGGGCGGATTTGATCCCATCGCCCAGGGATgcggcgg                                                                                                         >  1:1576404/1‑148 (MQ=255)
                                         ttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgt                                                                                                     >  2:1922882/1‑148 (MQ=255)
                                         ttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgt                                                                                                     <  2:2884746/148‑1 (MQ=255)
                                         ttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgt                                                                                                     >  2:3230605/1‑148 (MQ=255)
                                         ttttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgt                                                                                                     <  1:2370086/148‑1 (MQ=255)
                                          tttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgtg                                                                                                    <  2:3324917/148‑1 (MQ=255)
                                           ttGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGgtgt                                                                                                   >  2:1036199/1‑148 (MQ=255)
                                                   cAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCg                                                                                           >  1:3389933/1‑148 (MQ=255)
                                                      ccGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCa                                                                                        <  1:2871646/148‑1 (MQ=255)
                                                       cGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAg                                                                                       >  1:761171/1‑148 (MQ=255)
                                                          ggCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGc                                                                                    <  1:613840/148‑1 (MQ=255)
                                                          ggCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGc                                                                                    <  1:3158588/148‑1 (MQ=255)
                                                           gcagcaGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGTATGCGGCGGCGGTGTTTGTGCCGTCAGTGCt                                                                                   >  1:3187212/1‑148 (MQ=255)
                                                           gcagcaGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCt                                                                                   >  2:2926688/1‑148 (MQ=255)
                                                            cagcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCtt                                                                                  <  1:1997293/148‑1 (MQ=255)
                                                             agcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCttt                                                                                 <  2:676156/148‑1 (MQ=255)
                                                              gcagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTa                                                                                <  1:1258189/148‑1 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                               <  2:1111816/148‑1 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                               >  1:1168775/1‑148 (MQ=255)
                                                               cagTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTAc                                                                               >  1:2635167/1‑148 (MQ=255)
                                                                agTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACa                                                                              <  2:3401949/148‑1 (MQ=255)
                                                                  ttCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATg                                                                            >  2:2750436/1‑148 (MQ=255)
                                                                   tCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGc                                                                           >  2:1841053/1‑148 (MQ=255)
                                                                   tCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGc                                                                           >  1:1695974/1‑148 (MQ=255)
                                                                         tAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCa                                                                     >  1:2152825/1‑148 (MQ=255)
                                                                          aaCCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCaa                                                                    >  1:2914579/1‑148 (MQ=255)
                                                                           aCCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGTAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAAc                                                                   >  1:1608362/1‑148 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                 >  1:1747476/1‑148 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                 >  1:928305/1‑148 (MQ=255)
                                                                             cACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAg                                                                 <  1:299788/148‑1 (MQ=255)
                                                                              aCTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGa                                                                <  2:112544/148‑1 (MQ=255)
                                                                               cTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGAt                                                               >  1:837124/1‑148 (MQ=255)
                                                                                 gatgaGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTg                                                             >  1:2247968/1‑148 (MQ=255)
                                                                                           tACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACgcgc                                                   <  2:1288708/148‑1 (MQ=255)
                                                                                            aCGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                                                  >  2:1322581/1‑148 (MQ=255)
                                                                                            aCGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                                                  <  1:3685826/148‑1 (MQ=255)
                                                                                            aCGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCa                                                  >  2:2011251/1‑148 (MQ=255)
                                                                                               cccGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCagga                                               >  1:403304/1‑148 (MQ=255)
                                                                                               cccGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCagga                                               <  2:417282/148‑1 (MQ=255)
                                                                                                 cGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCaggagg                                             >  1:2928682/1‑148 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                      <  2:1396650/148‑1 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                      >  2:2265855/1‑148 (MQ=255)
                                                                                                        ggcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCcaacaa                                      <  1:568592/148‑1 (MQ=255)
                                                                                                         gcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaat                                     >  2:1810047/1‑148 (MQ=255)
                                                                                                         gcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaat                                     >  1:1831096/1‑148 (MQ=255)
                                                                                                         gcaggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaat                                     >  1:1887709/1‑148 (MQ=255)
                                                                                                           aggGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatga                                   <  1:2516773/148‑1 (MQ=255)
                                                                                                             ggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGTCCAACGCGCAGGAGGCCAACaatgaat                                 <  2:3085491/148‑1 (MQ=255)
                                                                                                             ggCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatgaat                                 <  1:3541464/148‑1 (MQ=255)
                                                                                                              gCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACaatgaatg                                <  2:373575/148‑1 (MQ=255)
                                                                                                                aaaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGac                              <  1:1063924/148‑1 (MQ=255)
                                                                                                                aaaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGac                              <  1:2686661/148‑1 (MQ=255)
                                                                                                                 aaCCATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGaca                             >  2:484105/1‑148 (MQ=255)
                                                                                                                   ccATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAcc                           <  1:3354861/148‑1 (MQ=255)
                                                                                                                   ccATTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACAcc                           >  2:3419900/1‑148 (MQ=255)
                                                                                                                     aTTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAg                         >  2:355358/1‑148 (MQ=255)
                                                                                                                     aTTGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAg                         >  1:2898232/1‑148 (MQ=255)
                                                                                                                       tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCt                       >  2:1077996/1‑148 (MQ=255)
                                                                                                                       tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCt                       >  2:3470911/1‑148 (MQ=255)
                                                                                                                       tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCt                       >  1:3304320/1‑148 (MQ=255)
                                                                                                                       tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCt                       >  1:2612535/1‑148 (MQ=255)
                                                                                                                       tGAAAACCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTCCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCt                       >  2:1199883/1‑148 (MQ=255)
                                                                                                                          aaaCCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCg                    >  2:822203/1‑148 (MQ=255)
                                                                                                                            aCCTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaa                  >  2:2680391/1‑148 (MQ=255)
                                                                                                                             ccTGTTTGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGaaa                 <  2:3052709/148‑1 (MQ=255)
                                                                                                                                  ttGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGc            >  1:2114367/1‑148 (MQ=255)
                                                                                                                                  ttGCCATCGGTTCGGAGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGc            >  1:2052484/1‑148 (MQ=255)
                                                                                                                                         cggttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGt     >  2:2475884/1‑148 (MQ=255)
                                                                                                                                         cggttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGt     >  1:1765458/1‑148 (MQ=255)
                                                                                                                                          ggttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTg    <  1:1699299/148‑1 (MQ=255)
                                                                                                                                           gttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGc   >  2:1428686/1‑148 (MQ=255)
                                                                                                                                            ttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCa  >  2:3646761/1‑148 (MQ=255)
                                                                                                                                            ttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCa  >  2:1805950/1‑148 (MQ=255)
                                                                                                                                            ttcggaGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCa  >  1:1495080/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGCGGATTTGATCCCATCGCCCAGGGATGCGGCGGCGGTGTTTGTGCCGTCAGTGCTTTACATGCCGCTCAACAGATTGCCCAACGCGCAGGAGGCCAACAATGAATGACACCAGCTTCGAAAACTGCATTAAGTGCA  >  CP009273/2348749‑2349036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑