Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,362,338 T→A 100% L274Q (CTG→CAG)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,362,3380TA99.5% 700.8 / ‑4.6 214L274Q (CTG→CAG) arnAfused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (107/106);  total (107/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 214 total aligned reads displayed.

TCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCG  >  CP009273/2362195‑2362481
                                                                                                                                               |                                                                                                                                               
tCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGaa                                                                                                                                             >  2:2331792/1‑148 (MQ=255)
tCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGaa                                                                                                                                             >  1:955400/1‑148 (MQ=255)
tCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGaa                                                                                                                                             >  2:2451687/1‑148 (MQ=255)
  cGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAAt                                                                                                                                           <  1:846283/148‑1 (MQ=255)
         gggTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCAcc                                                                                                                                    >  1:3242273/1‑148 (MQ=255)
            tGCCTTCAGCTATGTTGGCAATGAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGa                                                                                                                                 <  1:31556/148‑1 (MQ=255)
             gCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTAGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGAc                                                                                                                                <  1:647796/148‑1 (MQ=255)
              ccTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACa                                                                                                                               >  1:3505259/1‑148 (MQ=255)
               cTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAg                                                                                                                              <  1:2856805/148‑1 (MQ=255)
                 tCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggc                                                                                                                            <  1:2680363/148‑1 (MQ=255)
                  cAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggcg                                                                                                                           >  2:347143/1‑148 (MQ=255)
                     cTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAggcgggc                                                                                                                        >  2:2762080/1‑148 (MQ=255)
                       aTGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGa                                                                                                                      <  1:945031/148‑1 (MQ=255)
                        tGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGAc                                                                                                                     >  1:3583822/1‑148 (MQ=255)
                           tGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGc                                                                                                                  >  2:540655/1‑148 (MQ=255)
                            ggCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCa                                                                                                                 <  1:1726900/148‑1 (MQ=255)
                            ggCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCa                                                                                                                 <  2:2842120/148‑1 (MQ=255)
                            ggCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCa                                                                                                                 >  2:2359038/1‑148 (MQ=255)
                               aaTCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTa                                                                                                              <  1:1782350/148‑1 (MQ=255)
                                  cAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTa                                                                                                           >  2:745137/1‑148 (MQ=255)
                                   aGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTAt                                                                                                          >  2:1436584/1‑148 (MQ=255)
                                     aaaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGc                                                                                                        >  1:2988573/1‑148 (MQ=255)
                                      aaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCa                                                                                                       >  2:1407682/1‑148 (MQ=255)
                                      aaTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCa                                                                                                       <  2:1024043/148‑1 (MQ=255)
                                       aTTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAg                                                                                                      >  1:1511050/1‑148 (MQ=255)
                                           aCCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCt                                                                                                  <  1:116584/148‑1 (MQ=255)
                                               tCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCa                                                                                              >  2:304121/1‑148 (MQ=255)
                                                cTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCaa                                                                                             >  2:212938/1‑148 (MQ=255)
                                                cTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCaa                                                                                             >  1:3272792/1‑148 (MQ=255)
                                                    tcgtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAg                                                                                         <  2:1888919/148‑1 (MQ=255)
                                                    tcgtcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAg                                                                                         <  1:3092375/148‑1 (MQ=255)
                                                       tcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAgcgc                                                                                      >  2:1890413/1‑148 (MQ=255)
                                                       tcgCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAgcgc                                                                                      >  1:3645152/1‑148 (MQ=255)
                                                          cgTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGa                                                                                   <  2:192688/148‑1 (MQ=255)
                                                            tgtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACg                                                                                 <  1:3195086/148‑1 (MQ=255)
                                                             gtTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGc                                                                                >  2:901776/1‑148 (MQ=255)
                                                              ttCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCt                                                                               >  1:1020595/1‑148 (MQ=255)
                                                               tCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTg                                                                              <  2:560035/148‑1 (MQ=255)
                                                                 aTCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTggg                                                                            >  1:602921/1‑148 (MQ=255)
                                                                  tCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                           <  2:1111840/148‑1 (MQ=255)
                                                                  tCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                           <  1:3764108/148‑1 (MQ=255)
                                                                  tCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                           <  1:384352/148‑1 (MQ=255)
                                                                  tCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                           <  1:530963/148‑1 (MQ=255)
                                                                  tCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGc                                                                           <  1:2502871/148‑1 (MQ=255)
                                                                   ccTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGcc                                                                          <  1:2605556/148‑1 (MQ=255)
                                                                   ccTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGcc                                                                          >  1:3128292/1‑148 (MQ=255)
                                                                     tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTg                                                                        <  2:3242273/148‑1 (MQ=255)
                                                                     tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTg                                                                        >  1:1767588/1‑148 (MQ=255)
                                                                     tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTg                                                                        >  1:3121853/1‑148 (MQ=255)
                                                                     tCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTg                                                                        >  1:3356326/1‑148 (MQ=255)
                                                                         gCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGc                                                                    <  1:2858611/148‑1 (MQ=255)
                                                                             gCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAgg                                                                >  1:118926/1‑148 (MQ=255)
                                                                               aaaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGtt                                                              <  1:1882991/148‑1 (MQ=255)
                                                                                aaGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTc                                                             <  2:1829157/148‑1 (MQ=255)
                                                                                   cacaGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACg                                                          >  1:784262/1‑148 (MQ=255)
                                                                                     caGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTTGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCt                                                        <  1:1054644/148‑1 (MQ=255)
                                                                                     caGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCt                                                        >  2:1201697/1‑148 (MQ=255)
                                                                                       gCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTg                                                      >  2:1540146/1‑148 (MQ=255)
                                                                                        ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGa                                                     <  2:448612/148‑1 (MQ=255)
                                                                                        ccGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGa                                                     <  2:3016986/148‑1 (MQ=255)
                                                                                         cGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGaa                                                    <  2:1511366/148‑1 (MQ=255)
                                                                                         cGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGaa                                                    <  2:3583822/148‑1 (MQ=255)
                                                                                          ggggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAAt                                                   <  1:2249130/148‑1 (MQ=255)
                                                                                           gggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATa                                                  <  2:3741774/148‑1 (MQ=255)
                                                                                            ggAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATag                                                 >  1:1101452/1‑148 (MQ=255)
                                                                                             gAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagc                                                <  2:3093350/148‑1 (MQ=255)
                                                                                              aGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagcc                                               <  2:2790938/148‑1 (MQ=255)
                                                                                                cGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagccag                                             >  2:3658707/1‑148 (MQ=255)
                                                                                                cGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagccag                                             >  1:2298158/1‑148 (MQ=255)
                                                                                                 gTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagccagc                                            <  1:1798974/148‑1 (MQ=255)
                                                                                                 gTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagccagc                                            <  1:2698494/148‑1 (MQ=255)
                                                                                                  tGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATagccagcc                                           >  1:3552970/1‑148 (MQ=255)
                                                                                                     tttCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgc                                        <  1:2059499/148‑1 (MQ=255)
                                                                                                     tttCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgc                                        <  2:2275911/148‑1 (MQ=255)
                                                                                                      ttCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgcc                                       >  2:1272332/1‑148 (MQ=255)
                                                                                                      ttCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgcc                                       >  2:2655046/1‑148 (MQ=255)
                                                                                                       tCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAgcctgcct                                      >  1:1842015/1‑148 (MQ=255)
                                                                                                         tGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCctgcctgc                                    <  1:368185/148‑1 (MQ=255)
                                                                                                          gTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCa                                   <  1:1407682/148‑1 (MQ=255)
                                                                                                          gTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCa                                   <  2:3156305/148‑1 (MQ=255)
                                                                                                           ttGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCAc                                  <  2:603597/148‑1 (MQ=255)
                                                                                                           ttGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCAc                                  <  1:2571008/148‑1 (MQ=255)
                                                                                                              cgcCACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGc                               >  2:2207608/1‑148 (MQ=255)
                                                                                                                 cACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCg                            <  1:540655/148‑1 (MQ=255)
                                                                                                                 cACTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCg                            >  2:3338203/1‑148 (MQ=255)
                                                                                                                  aCTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGa                           >  2:2675042/1‑148 (MQ=255)
                                                                                                                  aCTGCTGATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGa                           >  1:815059/1‑148 (MQ=255)
                                                                                                                   ctgctgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGAc                          >  1:92031/1‑148 (MQ=255)
                                                                                                                     gctgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGc                        >  1:1216593/1‑148 (MQ=255)
                                                                                                                     gctgATTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGc                        >  1:3082380/1‑148 (MQ=255)
                                                                                                                         aTTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTa                    <  2:2215935/148‑1 (MQ=255)
                                                                                                                         aTTGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTa                    <  2:1721907/148‑1 (MQ=255)
                                                                                                                           tGCCTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTAcc                  <  1:2223109/148‑1 (MQ=255)
                                                                                                                             ccTGTGGCGATGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCg                <  2:2662536/148‑1 (MQ=255)
                                                                                                                                      aTGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCAt       >  2:2542086/1‑148 (MQ=255)
                                                                                                                                      aTGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCAt       >  2:1233555/1‑148 (MQ=255)
                                                                                                                                      aTGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCAt       <  2:406246/148‑1 (MQ=255)
                                                                                                                                      aTGGCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCAt       <  2:1980128/148‑1 (MQ=255)
                                                                                                                                        ggCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATcc     >  2:1609446/1‑148 (MQ=255)
                                                                                                                                        ggCGCGCAGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATcc     <  1:2360106/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TCCGTGGCCGGGTGCCTTCAGCTATGTTGGCAATCAGAAATTCACCGTCTGGTCGTCGCGTGTTCATCCTCATGCCAGCAAAGCACAGCCGGGGAGCGTGATTTCTGTTGCGCCACTGCTGATTGCCTGTGGCGATGGCGCGCTGGAAATCGTCACCGGACAGGCGGGCGACGGCATTACTATGCAGGGCTCGCAATTAGCGCAGACGCTGGGCCTGGTGCAAGGTTCACGCTTGAATAGCCAGCCTGCCTGCACCGCCCGACGCCGTACCCGGGTACTCATCCTCG  >  CP009273/2362195‑2362481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑