Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,422,841 C→A 100% D293Y (GAT→TAT)  purF ← amidophosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,422,8410CA99.6% 724.0 / ‑4.3 223D293Y (GAT→TAT) purFamidophosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (127/95);  total (127/96)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 223 total aligned reads displayed.

CTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCA  >  CP009273/2422698‑2422983
                                                                                                                                               |                                                                                                                                              
   gCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCAt                                                                                                                                         >  2:884865/1‑148 (MQ=255)
        gCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcgcg                                                                                                                                    >  2:3749467/1‑148 (MQ=255)
        gCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcgcg                                                                                                                                    >  1:2342909/1‑148 (MQ=255)
        gCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcgcg                                                                                                                                    >  2:2378036/1‑148 (MQ=255)
        gCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTcgcg                                                                                                                                    >  2:2377786/1‑148 (MQ=255)
         cATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCgg                                                                                                                                   >  1:3779392/1‑148 (MQ=255)
           tGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGc                                                                                                                                 >  1:310265/1‑148 (MQ=255)
           tGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGc                                                                                                                                 >  1:3763909/1‑148 (MQ=255)
            gATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCa                                                                                                                                <  2:333429/148‑1 (MQ=255)
            gATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCa                                                                                                                                >  1:55821/1‑148 (MQ=255)
             aTAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCaa                                                                                                                               >  1:30352/1‑148 (MQ=255)
              tataGGTGCGGCCAACATAGCGTTTTTTAACGAAGCCCTGGCGTTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCAAGATATTCCCATTCGCGGGCAAt                                                                                                                              <  1:1236263/145‑1 (MQ=255)
                 aGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAAtttt                                                                                                                           >  2:457565/1‑148 (MQ=255)
                  ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTtc                                                                                                                          <  1:2182477/148‑1 (MQ=255)
                  ggTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTtc                                                                                                                          <  1:2182126/148‑1 (MQ=255)
                     gCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCg                                                                                                                       <  2:362335/148‑1 (MQ=255)
                     gCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCg                                                                                                                       <  2:41034/148‑1 (MQ=255)
                       ggCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGcc                                                                                                                     <  2:1629784/148‑1 (MQ=255)
                            aCATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGtt                                                                                                                >  2:3499480/1‑148 (MQ=255)
                             cATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGttt                                                                                                               >  2:933806/1‑148 (MQ=255)
                             cATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTGTCTCGCCCAGttt                                                                                                               >  2:1621018/1‑148 (MQ=255)
                                aGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                            >  2:1089558/1‑148 (MQ=255)
                                aGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGt                                                                                                            <  1:3008389/148‑1 (MQ=255)
                                  cGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGc                                                                                                          >  2:272029/1‑148 (MQ=255)
                                  cGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGc                                                                                                          >  2:1404399/1‑148 (MQ=255)
                                     tttttAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCa                                                                                                       <  2:3401598/148‑1 (MQ=255)
                                          aaCGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATGTCCAGCGCGATATCACACGAGGTTTCTGGGANCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTc                                                                                                  <  1:1937839/148‑1 (MQ=255)
                                            cGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTcac                                                                                                <  2:2030120/148‑1 (MQ=255)
                                                gCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgc                                                                                            >  2:1135301/1‑148 (MQ=255)
                                                gCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgc                                                                                            >  1:2249816/1‑148 (MQ=255)
                                                   cTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgcgcg                                                                                         >  2:3196694/1‑148 (MQ=255)
                                                   cTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACAcgcgcg                                                                                         >  2:1293503/1‑148 (MQ=255)
                                                    tGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACgcgcgc                                                                                        <  2:2137675/148‑1 (MQ=255)
                                                    tGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACgcgcgc                                                                                        >  2:781333/1‑148 (MQ=255)
                                                      gCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTg                                                                                      >  2:2727659/1‑148 (MQ=255)
                                                       cGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGt                                                                                     >  1:3542866/1‑148 (MQ=255)
                                                        ggTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTa                                                                                    <  1:2906424/148‑1 (MQ=255)
                                                         gTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTaa                                                                                   <  1:1333623/148‑1 (MQ=255)
                                                           aCGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAAc                                                                                 <  1:2831422/148‑1 (MQ=255)
                                                           aCGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAAc                                                                                 >  2:101835/1‑148 (MQ=255)
                                                            cGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACg                                                                                >  1:389223/1‑148 (MQ=255)
                                                              gTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGa                                                                              <  1:680588/148‑1 (MQ=255)
                                                               tttGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaa                                                                             <  2:851383/148‑1 (MQ=255)
                                                                ttGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaaa                                                                            >  1:2631636/1‑148 (MQ=255)
                                                                ttGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGaaa                                                                            >  2:2739587/1‑148 (MQ=255)
                                                                       gAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGGTCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTc                                                                     >  2:1215815/1‑148 (MQ=255)
                                                                       gAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTc                                                                     >  1:3428780/1‑148 (MQ=255)
                                                                         aTACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGa                                                                   <  2:310265/148‑1 (MQ=255)
                                                                          tACTAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                  >  2:2312488/1‑148 (MQ=255)
                                                                          tACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                  >  1:3711424/1‑148 (MQ=255)
                                                                          tACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGAt                                                                  >  1:3637422/1‑148 (MQ=255)
                                                                              aGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAAc                                                              >  2:3537370/1‑148 (MQ=255)
                                                                              aGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAAc                                                              >  1:2464346/1‑148 (MQ=255)
                                                                                cAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGa                                                            >  1:1864523/1‑148 (MQ=255)
                                                                                 aaTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAg                                                           <  1:2377804/148‑1 (MQ=255)
                                                                                 aaTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAg                                                           <  1:2946452/148‑1 (MQ=255)
                                                                                 aaTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAg                                                           <  1:1038021/148‑1 (MQ=255)
                                                                                  aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGt                                                          >  2:3253716/1‑148 (MQ=255)
                                                                                  aTTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGt                                                          >  2:2090141/1‑148 (MQ=255)
                                                                                       cAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                     <  1:2317017/148‑1 (MQ=255)
                                                                                       cAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCcggg                                                     <  1:3457125/148‑1 (MQ=255)
                                                                                              atatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATACACGAGTCCGGGCGGGCaa                                              <  1:350435/148‑1 (MQ=255)
                                                                                              atatCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCaa                                              >  1:2117798/1‑148 (MQ=255)
                                                                                                 tCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                           <  2:2854042/148‑1 (MQ=255)
                                                                                                 tCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGt                                           >  2:504348/1‑148 (MQ=255)
                                                                                                  cacaCGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGta                                          >  2:246824/1‑148 (MQ=255)
                                                                                                  cacaCGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATCTTGTCGATAAACGAGTCCGGGCGGGCAAAGta                                          >  1:543145/1‑148 (MQ=255)
                                                                                                    cacGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGtata                                        <  2:739829/148‑1 (MQ=255)
                                                                                                      cGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTataca                                      <  2:1713828/148‑1 (MQ=255)
                                                                                                      cGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTataca                                      <  2:3692344/148‑1 (MQ=255)
                                                                                                        aGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacata                                    <  1:2233683/148‑1 (MQ=255)
                                                                                                        aGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTatacata                                    <  1:3308670/148‑1 (MQ=255)
                                                                                                          gTTTCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACt                                  <  2:605859/148‑1 (MQ=255)
                                                                                                           tttCTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTc                                 >  2:3680592/1‑148 (MQ=255)
                                                                                                            ttCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCa                                <  2:750810/148‑1 (MQ=255)
                                                                                                              cTGGGATCGGGATCACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCaaa                              <  2:1431486/148‑1 (MQ=255)
                                                                                                                 ggatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAg                           >  2:1856644/1‑148 (MQ=255)
                                                                                                                  gatcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAgg                          <  2:3637422/148‑1 (MQ=255)
                                                                                                                   atcgggatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGc                         <  2:30352/148‑1 (MQ=255)
                                                                                                                        gatcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGa                    <  2:2631636/148‑1 (MQ=255)
                                                                                                                         atcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAt                   <  1:50904/148‑1 (MQ=255)
                                                                                                                          tcACCACGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGAtt                  >  1:3520842/1‑148 (MQ=255)
                                                                                                                           caccacGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTg                 <  1:457565/148‑1 (MQ=255)
                                                                                                                             ccacGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCt               >  2:861178/1‑148 (MQ=255)
                                                                                                                              cacGTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTg              >  1:2381395/1‑148 (MQ=255)
                                                                                                                                 gTCGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGAcc           <  1:884865/148‑1 (MQ=255)
                                                                                                                                   cGATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCgg         <  2:3779392/148‑1 (MQ=255)
                                                                                                                                    gATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGa        >  1:179284/1‑148 (MQ=255)
                                                                                                                                    gATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGa        <  1:142777/148‑1 (MQ=255)
                                                                                                                                    gATATCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGa        >  1:179528/1‑148 (MQ=255)
                                                                                                                                       atCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTg     >  1:2666007/1‑148 (MQ=255)
                                                                                                                                       atCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTg     <  1:949336/148‑1 (MQ=255)
                                                                                                                                        tCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt    <  1:3230267/148‑1 (MQ=255)
                                                                                                                                        tCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt    <  1:3196694/148‑1 (MQ=255)
                                                                                                                                        tCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt    >  1:1287959/1‑148 (MQ=255)
                                                                                                                                        tCCAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGt    <  1:2746474/148‑1 (MQ=255)
                                                                                                                                         ccAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc   >  2:2966976/1‑148 (MQ=255)
                                                                                                                                         ccAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc   <  1:625222/148‑1 (MQ=255)
                                                                                                                                         ccAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTc   <  1:625260/148‑1 (MQ=255)
                                                                                                                                          cAGATATTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCa  >  2:102688/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CTGGCCCGGCATGATAAAGGTGCGGCCAACATAGCGGTTTTTAACGAAGCCCTGGCGGTACGGTTTGCCCAGAATACGAGCAATTTCCAGCGCGATATCACACGAGGTTTCTGGGATCGGGATCACCACGTCGATATCCAGATCTTCCCATTCGCGGGCAATTTTCTCGCCCAGTTTCGTGCCCATATTCACACGCGCGCTGTAAACGGAAATTTTGTCGATAAACGAGTCCGGGCGGGCAAAGTATACATACTCAAACAGGCACGGATTGCTGACCGGATTGTCA  >  CP009273/2422698‑2422983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑