Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,424,074 C→T 100% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,424,0740CT100.0% 667.5 / NA 205D57N (GAC→AAC) cvpAcolicin V production membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (94/111);  total (94/111)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAT  >  CP009273/2423929‑2424218
                                                                                                                                                 |                                                                                                                                                
   aaaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaa                                                                                                                                             >  2:73555/1‑148 (MQ=255)
    aaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCaaa                                                                                                                                            <  2:2089938/148‑1 (MQ=255)
    aaCAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACAAGTTCGTTTTCaaa                                                                                                                                            >  2:3507565/1‑148 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               <  2:1667452/148‑1 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               >  1:1776393/1‑148 (MQ=255)
                 cACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               >  1:1946089/1‑148 (MQ=255)
                 cACCCGATCGGTGCCTGAAAACCCAGTTTTCTCCAACAACTGGACTATCACGAAGTTCACGATAGCACCATCGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCAATTTCGAACAATTTCGTTTTCAAAGCCCGTAAACCAg                                                                                                                               <  2:2752317/148‑1 (MQ=255)
                  aCCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGa                                                                                                                              <  2:2769769/148‑1 (MQ=255)
                      gATCGGGGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGa                                                                                                                          <  1:26071/148‑1 (MQ=255)
                         cGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAg                                                                                                                       <  1:293113/148‑1 (MQ=255)
                           gTGGCTGACATCGCCGTTTTCTCCTCAAAGTGGCCTATCACGAAGTTGACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTAAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     <  2:1112350/148‑1 (MQ=255)
                           gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     <  2:3019984/148‑1 (MQ=255)
                           gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     <  1:308102/148‑1 (MQ=255)
                           gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     >  1:165232/1‑148 (MQ=255)
                           gTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGt                                                                                                                     >  2:1582024/1‑148 (MQ=255)
                              ccTTACAACCCCGTTTTCTCCACCAACTGGCCTCTCACTAAGTTCACGAAAGCACCAACGAACAGGGTAGCGATAAACAGTACCGCGATGGCAAACCCATATCGAACCAGATCGTTTTCAAAGCCAGTAAACCAGACTGACAGGTAAg                                                                                                                  >  1:139179/1‑148 (MQ=255)
                              ccTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAg                                                                                                                  <  1:203371/148‑1 (MQ=255)
                                tGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtg                                                                                                                <  1:3577470/148‑1 (MQ=255)
                                 gACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtgt                                                                                                               <  2:1476079/148‑1 (MQ=255)
                                 gACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAgtgt                                                                                                               <  2:662926/148‑1 (MQ=255)
                                  aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgta                                                                                                              >  1:1179033/1‑148 (MQ=255)
                                  aCAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgta                                                                                                              <  1:239096/148‑1 (MQ=255)
                                    aaCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagt                                                                                                            >  2:3518802/1‑148 (MQ=255)
                                     aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                           <  1:859789/148‑1 (MQ=255)
                                     aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                           <  1:2655138/148‑1 (MQ=255)
                                     aCCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTgtagta                                                                                                           <  1:2857872/148‑1 (MQ=255)
                                        ccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                        <  1:2081270/148‑1 (MQ=255)
                                        ccGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATg                                                                                                        <  1:1980376/148‑1 (MQ=255)
                                         cGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGa                                                                                                       >  2:1521803/1‑148 (MQ=255)
                                         cGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGa                                                                                                       <  1:2665621/148‑1 (MQ=255)
                                          gTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGAc                                                                                                      <  1:1884641/148‑1 (MQ=255)
                                              tctcCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGc                                                                                                  >  1:705148/1‑148 (MQ=255)
                                                 ccaccaACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGcaac                                                                                               >  1:2958516/1‑148 (MQ=255)
                                                       aCTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACaaagaa                                                                                         <  1:1356882/148‑1 (MQ=255)
                                                          ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGc                                                                                      <  2:3761604/148‑1 (MQ=255)
                                                          ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGc                                                                                      <  2:1817582/148‑1 (MQ=255)
                                                          ggCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGc                                                                                      <  2:3748656/148‑1 (MQ=255)
                                                              tATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACaa                                                                                  <  2:3545392/148‑1 (MQ=255)
                                                                tCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAAcc                                                                                >  2:3365706/1‑148 (MQ=255)
                                                                   cGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCa                                                                             >  1:322052/1‑148 (MQ=255)
                                                                    gAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAt                                                                            <  1:527808/148‑1 (MQ=255)
                                                                    gAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCAt                                                                            <  1:2761710/148‑1 (MQ=255)
                                                                     aaGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATg                                                                           <  1:2283233/148‑1 (MQ=255)
                                                                       gTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTc                                                                         >  1:217102/1‑148 (MQ=255)
                                                                        ttCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCa                                                                        <  2:2009300/148‑1 (MQ=255)
                                                                         tCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAc                                                                       >  1:2227530/1‑148 (MQ=255)
                                                                         tCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCAc                                                                       >  2:1202825/1‑148 (MQ=255)
                                                                              aTAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCg                                                                  >  1:3487263/1‑148 (MQ=255)
                                                                               tAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                 <  2:530799/148‑1 (MQ=255)
                                                                               tAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                 <  2:2451349/148‑1 (MQ=255)
                                                                               tAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGa                                                                 >  2:2203537/1‑148 (MQ=255)
                                                                                  cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATaa                                                              >  1:2000738/1‑148 (MQ=255)
                                                                                  cACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATaa                                                              >  2:2812643/1‑148 (MQ=255)
                                                                                    ccAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACg                                                            <  2:241145/148‑1 (MQ=255)
                                                                                    ccAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACg                                                            <  2:240733/148‑1 (MQ=255)
                                                                                     cAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGc                                                           >  2:3733743/1‑148 (MQ=255)
                                                                                            cAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGa                                                    >  2:1685633/1‑148 (MQ=255)
                                                                                                gTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaa                                                <  2:2820204/148‑1 (MQ=255)
                                                                                                gTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACaa                                                <  2:3132986/148‑1 (MQ=255)
                                                                                                     gATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcg                                           <  2:213939/148‑1 (MQ=255)
                                                                                                     gATAAACAGTACCGCGATGGATATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCcg                                           <  2:2081833/148‑1 (MQ=255)
                                                                                                        aaaCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCgg                                        >  2:3262551/1‑148 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      <  2:2657233/148‑1 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      >  2:1064171/1‑148 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      >  2:2843856/1‑148 (MQ=255)
                                                                                                          aCAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGAt                                      >  2:2843911/1‑148 (MQ=255)
                                                                                                           cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                     >  2:2341890/1‑148 (MQ=255)
                                                                                                           cAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATc                                     >  2:319201/1‑148 (MQ=255)
                                                                                                              tACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAgg                                  <  2:1412934/148‑1 (MQ=255)
                                                                                                              tACCGCGATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAgg                                  <  1:3748960/148‑1 (MQ=255)
                                                                                                                 cgcgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTa                               <  2:2789947/148‑1 (MQ=255)
                                                                                                                 cgcgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTa                               <  2:945544/148‑1 (MQ=255)
                                                                                                                  gcgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTaa                              <  2:1522812/148‑1 (MQ=255)
                                                                                                                  gcgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTaa                              <  2:1036226/148‑1 (MQ=255)
                                                                                                                   cgATGGCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAAc                             >  2:3415339/1‑148 (MQ=255)
                                                                                                                       ggCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCaga                         >  2:950304/1‑148 (MQ=255)
                                                                                                                       ggCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCaga                         >  1:299848/1‑148 (MQ=255)
                                                                                                                        gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGGTCAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                        <  1:3413981/148‑1 (MQ=255)
                                                                                                                        gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                        >  1:1838497/1‑148 (MQ=255)
                                                                                                                        gCAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagag                        >  1:3729336/1‑148 (MQ=255)
                                                                                                                         cAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagaga                       <  2:2031604/148‑1 (MQ=255)
                                                                                                                         cAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagaga                       <  2:837515/148‑1 (MQ=255)
                                                                                                                         cAATCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCagaga                       <  2:3468461/148‑1 (MQ=255)
                                                                                                                           aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAgg                     >  1:714068/1‑148 (MQ=255)
                                                                                                                           aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAgg                     <  1:620079/148‑1 (MQ=255)
                                                                                                                           aTCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAgg                     >  1:3684736/1‑148 (MQ=255)
                                                                                                                            tCCCATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGa                    >  2:2023489/1‑148 (MQ=255)
                                                                                                                               cATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaa                 >  2:3338381/1‑148 (MQ=255)
                                                                                                                               cATTTCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGaaaa                 <  2:483842/148‑1 (MQ=255)
                                                                                                                                 tttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAg               >  2:3550263/1‑148 (MQ=255)
                                                                                                                                 tttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAg               >  2:710214/1‑148 (MQ=255)
                                                                                                                                  ttCGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGc              <  2:1637784/148‑1 (MQ=255)
                                                                                                                                    cGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACACAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCaa            <  2:137105/148‑1 (MQ=255)
                                                                                                                                    cGAACCAGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCaa            <  1:1202825/148‑1 (MQ=255)
                                                                                                                                          agttagttTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCATTCACcg      <  2:51791/148‑1 (MQ=255)
                                                                                                                                          aGTTCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACcg      <  2:3487213/148‑1 (MQ=255)
                                                                                                                                            ttCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACcgcg    <  2:893816/148‑1 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   >  2:683450/1‑148 (MQ=255)
                                                                                                                                             tCGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGa   <  2:1156547/148‑1 (MQ=255)
                                                                                                                                              cGTTTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAt  <  2:338466/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGAAACAGACGCCCAGCACCCGATCGGTGCCTGACAACCCCGTTTTCTCCACCAACTGGCCTATCACGAAGTTCACGATAGCACCAACGATCAGGGTAGCGATAAACAGTACCGCGATGGCAATCCCATTTCGAACCAGTTCGTCTTCAAAGCCCGTAAACCAGACTGACAGGTAAGTGTAGTAATGACTGGCAACAAAGAAAGCACAACCCCATGTCACCAGCGATAACGCTTCACGAACAAAGCCGCGGATCAGGCTAACCAGAGAGGAAAAAGCAATCACCGCGAT  >  CP009273/2423929‑2424218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑