Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 25,546 G→T 100% D114Y (GAC→TAC)  lspA → prolipoprotein signal peptidase (signal peptidase II)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927325,5460GT100.0% 623.3 / ‑6.7 191D114Y (GAC→TAC) lspAprolipoprotein signal peptidase (signal peptidase II)
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (114/75);  total (115/76)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 191 total aligned reads displayed.

GCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGA  >  CP009273/25403‑25690
                                                                                                                                               |                                                                                                                                                
gcTGGCAGCGTTGGTTCTTTGCCGGTATTGCGCTTGGTAATAGCGAGATCCTGGCAGTGATGATGTATCGCTCGAAGTCCACGCAGAAGCTAAACAAAATCGCTAACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCAAcag                                                                                                                                              >  1:2472540/1‑146 (MQ=255)
   ggCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCt                                                                                                                                           >  1:2301905/1‑148 (MQ=255)
    gCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCtg                                                                                                                                          >  2:2540466/1‑148 (MQ=255)
       gCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTgg                                                                                                                                       <  2:1319102/148‑1 (MQ=255)
            ggTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACgg                                                                                                                                  <  1:1373597/148‑1 (MQ=255)
             gTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGc                                                                                                                                 >  1:2237752/1‑148 (MQ=255)
              ttCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCt                                                                                                                                >  2:3766092/1‑148 (MQ=255)
               tCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCtt                                                                                                                               >  2:261615/1‑148 (MQ=255)
               tCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCtt                                                                                                                               >  2:135725/1‑148 (MQ=255)
                   tGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGtt                                                                                                                           <  2:1908002/148‑1 (MQ=255)
                   tGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGtt                                                                                                                           <  2:1908825/148‑1 (MQ=255)
                         tATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGat                                                                                                                     >  1:747788/1‑148 (MQ=255)
                         tATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGat                                                                                                                     <  1:2493058/148‑1 (MQ=255)
                            tGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATg                                                                                                                  <  2:2528901/148‑1 (MQ=255)
                            tGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATg                                                                                                                  >  1:2433971/1‑148 (MQ=255)
                              cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGAt                                                                                                                <  2:3805225/148‑1 (MQ=255)
                              cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGAt                                                                                                                <  1:994692/148‑1 (MQ=255)
                              cGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGAt                                                                                                                <  2:2320207/148‑1 (MQ=255)
                                aTTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCg                                                                                                              >  1:1055393/1‑148 (MQ=255)
                                 ttGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGa                                                                                                             >  2:862081/1‑148 (MQ=255)
                                  tGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGAc                                                                                                            >  1:3649027/1‑148 (MQ=255)
                                    gTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACtt                                                                                                          <  2:3586199/148‑1 (MQ=255)
                                     tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                         <  1:3725409/148‑1 (MQ=255)
                                     tATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTc                                                                                                         <  1:3150061/148‑1 (MQ=255)
                                       ttAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                       <  2:1393462/148‑1 (MQ=255)
                                       ttAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTa                                                                                                       <  1:1780481/148‑1 (MQ=255)
                                         aGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACg                                                                                                     <  1:734896/148‑1 (MQ=255)
                                              gATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGc                                                                                                >  2:616656/1‑148 (MQ=255)
                                                    ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTgg                                                                                          >  1:238224/1‑148 (MQ=255)
                                                    ggCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTgg                                                                                          <  1:1620551/148‑1 (MQ=255)
                                                         tgatgaTGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACtt                                                                                     <  2:2352823/148‑1 (MQ=255)
                                                           atgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                   >  2:2902155/1‑148 (MQ=255)
                                                           atgatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCg                                                                                   >  2:510511/1‑148 (MQ=255)
                                                             gatgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGcc                                                                                 >  1:1576578/1‑148 (MQ=255)
                                                              atgTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCa                                                                                <  1:677818/148‑1 (MQ=255)
                                                                gTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCAcc                                                                              <  2:2962850/148‑1 (MQ=255)
                                                                gTATCGCTAGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCAcc                                                                              >  2:3257014/1‑148 (MQ=255)
                                                                       tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAAcc                                                                       <  1:1049517/148‑1 (MQ=255)
                                                                       tCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAAcc                                                                       <  1:2958465/148‑1 (MQ=255)
                                                                        cGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCt                                                                      >  2:2290885/1‑148 (MQ=255)
                                                                          aaGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTg                                                                    >  2:2508889/1‑148 (MQ=255)
                                                                           aGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGc                                                                   >  2:3465261/1‑148 (MQ=255)
                                                                            ggCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGcc                                                                  >  2:1656908/1‑148 (MQ=255)
                                                                               cACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGAt                                                               <  2:2362110/148‑1 (MQ=255)
                                                                               cACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGAt                                                               <  1:1299206/148‑1 (MQ=255)
                                                                                  gCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACt                                                            <  1:3385772/148‑1 (MQ=255)
                                                                                    aGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGc                                                          >  2:3098726/1‑148 (MQ=255)
                                                                                     gAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                         >  2:1826908/1‑148 (MQ=255)
                                                                                     gAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                         >  2:1618673/1‑148 (MQ=255)
                                                                                     gAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGcc                                                         >  2:3380613/1‑148 (MQ=255)
                                                                                      aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                        >  1:3789152/1‑148 (MQ=255)
                                                                                      aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                        >  1:3528182/1‑148 (MQ=255)
                                                                                      aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                        >  1:1599832/1‑148 (MQ=255)
                                                                                      aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                        >  2:3058290/1‑148 (MQ=255)
                                                                                      aaGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCa                                                        >  1:3527886/1‑148 (MQ=255)
                                                                                          tAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                    >  2:945149/1‑148 (MQ=255)
                                                                                           aaaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgt                                                   >  1:2780303/1‑148 (MQ=255)
                                                                                            aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                  >  1:3027945/1‑148 (MQ=255)
                                                                                            aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                  <  1:513405/148‑1 (MQ=255)
                                                                                            aaCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                  <  1:3701025/148‑1 (MQ=255)
                                                                                             aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                 >  1:1068236/1‑148 (MQ=255)
                                                                                             aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                 >  2:2766612/1‑148 (MQ=255)
                                                                                             aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                 >  1:3331520/1‑148 (MQ=255)
                                                                                             aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                 >  2:3340375/1‑148 (MQ=255)
                                                                                             aCAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTgtgt                                                 <  1:3512605/148‑1 (MQ=255)
                                                                                              cAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTc                                                >  1:2613412/1‑148 (MQ=255)
                                                                                                      cTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGc                                        >  2:1232322/1‑148 (MQ=255)
                                                                                                       ttACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCa                                       <  2:1540514/148‑1 (MQ=255)
                                                                                                        tACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCAc                                      >  1:2808579/1‑148 (MQ=255)
                                                                                                          cgcgCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTg                                    <  1:3507966/148‑1 (MQ=255)
                                                                                                            cgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAt                                  <  2:1890048/148‑1 (MQ=255)
                                                                                                            cgcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAt                                  <  2:2099706/148‑1 (MQ=255)
                                                                                                             gcTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGAtt                                 <  1:2982118/148‑1 (MQ=255)
                                                                                                              cTGATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtg                                <  1:682969/148‑1 (MQ=255)
                                                                                                                gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                              <  1:1382711/148‑1 (MQ=255)
                                                                                                                gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                              <  1:3332853/148‑1 (MQ=255)
                                                                                                                gATTATTGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgtg                              >  2:1321371/1‑148 (MQ=255)
                                                                                                                 attattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGc                             >  2:555641/1‑148 (MQ=255)
                                                                                                                   tattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                           >  1:1369404/1‑148 (MQ=255)
                                                                                                                   tattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                           >  1:3001175/1‑148 (MQ=255)
                                                                                                                   tattGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTg                           >  2:2432006/1‑148 (MQ=255)
                                                                                                                     ttGGCGGCGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGAACTGATTGTGCTGGa                         <  1:135725/148‑1 (MQ=255)
                                                                                                                       ggcggcGCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAg                       <  2:1489296/148‑1 (MQ=255)
                                                                                                                         cggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                     <  2:2192593/148‑1 (MQ=255)
                                                                                                                          ggcgCGCTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATATTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtt                    <  1:1173744/148‑1 (MQ=255)
                                                                                                                            cgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                  <  1:1721746/148‑1 (MQ=255)
                                                                                                                            cgcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGtttt                  <  2:1990548/148‑1 (MQ=255)
                                                                                                                             gcgcTGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGttttt                 <  1:3154719/148‑1 (MQ=255)
                                                                                                                                 tGGGCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGcc             <  2:2455359/148‑1 (MQ=255)
                                                                                                                                   ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCtt           >  2:3536768/1‑148 (MQ=255)
                                                                                                                                   ggCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCtt           >  2:1471377/1‑148 (MQ=255)
                                                                                                                                    gCAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTc          >  1:2067100/1‑148 (MQ=255)
                                                                                                                                     cAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt         <  1:1904191/148‑1 (MQ=255)
                                                                                                                                     cAACCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCt         <  2:1055393/148‑1 (MQ=255)
                                                                                                                                       aCCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTag       >  2:1025228/1‑148 (MQ=255)
                                                                                                                                       aCCTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTag       <  1:2617024/148‑1 (MQ=255)
                                                                                                                                        ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTaga      >  2:3292716/1‑148 (MQ=255)
                                                                                                                                        ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTaga      >  1:1013705/1‑148 (MQ=255)
                                                                                                                                        ccTGTTCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTaga      >  1:1013168/1‑148 (MQ=255)
                                                                                                                                            ttCTACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGa  <  1:2700846/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GCTGGCAGCGTTGGTTCTTTGCCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACCTGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGA  >  CP009273/25403‑25690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑