Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,589,933 G→A 100% T55I (ACC→ATC)  ypfJ ← putative neutral zinc metallopeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,589,9330GA100.0% 875.5 / NA 260T55I (ACC→ATC) ypfJputative neutral zinc metallopeptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (144/116);  total (144/116)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 260 total aligned reads displayed.

TTACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTCAC  >  CP009273/2589789‑2590077
                                                                                                                                                |                                                                                                                                                
 tACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTaa                                                                                                                                              >  2:2079446/1‑148 (MQ=255)
 tACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTaa                                                                                                                                              >  2:3040134/1‑148 (MQ=255)
    ccATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATc                                                                                                                                           <  1:59678/148‑1 (MQ=255)
    ccATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATc                                                                                                                                           >  2:2475362/1‑148 (MQ=255)
    ccATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATc                                                                                                                                           <  1:1990511/148‑1 (MQ=255)
       tcttctCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAac                                                                                                                                        <  2:2022010/148‑1 (MQ=255)
        cttctCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAaca                                                                                                                                       >  2:1608977/1‑148 (MQ=255)
        cttctCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAaca                                                                                                                                       >  2:3296035/1‑148 (MQ=255)
        cttctCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAaca                                                                                                                                       <  2:3207884/148‑1 (MQ=255)
          tctcGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACAcc                                                                                                                                     <  2:3606966/148‑1 (MQ=255)
            tcGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAt                                                                                                                                   <  2:683477/148‑1 (MQ=255)
             cGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAta                                                                                                                                  >  1:865690/1‑148 (MQ=255)
             cGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCAta                                                                                                                                  >  2:2496014/1‑148 (MQ=255)
                  tgttgtCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGc                                                                                                                             <  1:313460/148‑1 (MQ=255)
                    ttgtCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCt                                                                                                                           >  1:2451777/1‑148 (MQ=255)
                       tCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCa                                                                                                                        >  1:463315/1‑148 (MQ=255)
                       tCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCa                                                                                                                        <  1:1659210/148‑1 (MQ=255)
                       tCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCa                                                                                                                        >  1:2384926/1‑148 (MQ=255)
                          ccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAAcc                                                                                                                     >  2:1253351/1‑148 (MQ=255)
                          ccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAAcc                                                                                                                     <  2:831839/148‑1 (MQ=255)
                          ccAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAAGTGTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAAcc                                                                                                                     <  2:831904/148‑1 (MQ=255)
                             ggTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGc                                                                                                                  >  1:342034/1‑148 (MQ=255)
                             ggTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGc                                                                                                                  <  1:1239672/148‑1 (MQ=255)
                              gtgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCa                                                                                                                 >  1:1959607/1‑148 (MQ=255)
                              gtgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCa                                                                                                                 >  1:1066149/1‑148 (MQ=255)
                               tgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCAc                                                                                                                >  2:3049708/1‑148 (MQ=255)
                               tgtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCAc                                                                                                                >  2:3705853/1‑148 (MQ=255)
                                gtCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACg                                                                                                               <  1:45856/148‑1 (MQ=255)
                                 tCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGa                                                                                                              >  1:2656080/1‑148 (MQ=255)
                                 tCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGa                                                                                                              <  1:2146696/148‑1 (MQ=255)
                                  cTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGAc                                                                                                             >  1:1376891/1‑148 (MQ=255)
                                       gTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCa                                                                                                        <  2:1707564/148‑1 (MQ=255)
                                         ggTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGt                                                                                                      >  1:1499050/1‑148 (MQ=255)
                                             gCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTaaaa                                                                                                  <  2:931819/148‑1 (MQ=255)
                                               cAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATc                                                                                                <  1:1187484/148‑1 (MQ=255)
                                                aGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCa                                                                                               >  1:2674855/1‑148 (MQ=255)
                                                  aaTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGc                                                                                             <  2:1030771/148‑1 (MQ=255)
                                                  aaTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGc                                                                                             >  1:2079275/1‑148 (MQ=255)
                                                  aaTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACACGATTAAATCAACACCATAGTAGACTGAAACCAGCACGACTATCAGTAAAATCAGc                                                                                             >  2:3600656/1‑148 (MQ=255)
                                                   aTCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGcc                                                                                            <  2:80291/148‑1 (MQ=255)
                                                    tCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTaaa                                                                                                   >  1:3021972/1‑140 (MQ=255)
                                                       ccGCGGGGAATGTTGCGGCTTCGTCGTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                        <  2:2121262/148‑1 (MQ=255)
                                                       ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                        <  1:102701/148‑1 (MQ=255)
                                                       ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                        <  2:1811261/148‑1 (MQ=255)
                                                       ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                        >  2:3073982/1‑148 (MQ=255)
                                                       ccGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGcc                                                                                        <  2:436963/148‑1 (MQ=255)
                                                        cGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCt                                                                                       <  2:3364393/148‑1 (MQ=255)
                                                          aGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCttt                                                                                     >  1:3513810/1‑148 (MQ=255)
                                                              gAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCg                                                                                 <  1:770393/148‑1 (MQ=255)
                                                               aaTTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGc                                                                                <  1:3524199/148‑1 (MQ=255)
                                                                aTTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCt                                                                               >  2:1781588/1‑148 (MQ=255)
                                                                  tttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTg                                                                             >  2:2596266/1‑148 (MQ=255)
                                                                  tttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTg                                                                             >  2:2767993/1‑148 (MQ=255)
                                                                  tttGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTg                                                                             >  1:130560/1‑148 (MQ=255)
                                                                          ttCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGa                                                                     >  2:1105507/1‑148 (MQ=255)
                                                                           tCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGaa                                                                    >  2:2367467/1‑148 (MQ=255)
                                                                            cGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGaaa                                                                   >  1:1420103/1‑148 (MQ=255)
                                                                              tCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAc                                                                 >  1:2010559/1‑148 (MQ=255)
                                                                              tCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAc                                                                 >  1:2554204/1‑148 (MQ=255)
                                                                              tCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAc                                                                 >  1:2722599/1‑148 (MQ=255)
                                                                               cTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAcc                                                                >  2:308677/1‑148 (MQ=255)
                                                                                  cATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCggg                                                             <  1:1523969/148‑1 (MQ=255)
                                                                                   aTTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGa                                                            >  1:2336650/1‑148 (MQ=255)
                                                                                        ggCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGcc                                                       <  2:115257/148‑1 (MQ=255)
                                                                                        ggCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGcc                                                       <  2:2982068/148‑1 (MQ=255)
                                                                                         gCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGccc                                                      <  2:386320/148‑1 (MQ=255)
                                                                                          cTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCa                                                     <  1:3705853/148‑1 (MQ=255)
                                                                                             aTTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAg                                                  >  2:1944685/1‑148 (MQ=255)
                                                                                              ttGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGa                                                 >  1:24410/1‑148 (MQ=255)
                                                                                               tGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGAt                                                >  1:913227/1‑148 (MQ=255)
                                                                                               tGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGAt                                                <  2:508070/148‑1 (MQ=255)
                                                                                                gACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATg                                               >  1:3346298/1‑148 (MQ=255)
                                                                                                    gcgTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGacc                                           <  2:1376891/148‑1 (MQ=255)
                                                                                                       ttGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGaccacc                                        >  1:655031/1‑148 (MQ=255)
                                                                                                        tGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGAccacca                                       >  2:1635518/1‑148 (MQ=255)
                                                                                                            tgttgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGc                                   >  1:2834005/1‑148 (MQ=255)
                                                                                                            tgttgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGc                                   >  1:3217013/1‑148 (MQ=255)
                                                                                                               tgttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGt                                <  2:2992320/148‑1 (MQ=255)
                                                                                                                gttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGtt                               <  1:1846397/148‑1 (MQ=255)
                                                                                                                gttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGtt                               <  2:1424747/148‑1 (MQ=255)
                                                                                                                 ttgGGAAACCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTg                              <  2:1500792/148‑1 (MQ=255)
                                                                                                                        aCCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGt                       >  2:634501/1‑148 (MQ=255)
                                                                                                                        aCCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGt                       <  1:387602/148‑1 (MQ=255)
                                                                                                                        aCCGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGt                       <  1:3110210/148‑1 (MQ=255)
                                                                                                                         ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCACCCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                      <  2:2943046/148‑1 (MQ=255)
                                                                                                                         ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCagag                                    <  2:2505261/134‑1 (MQ=255)
                                                                                                                         ccGGCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTc                      <  1:3040134/148‑1 (MQ=255)
                                                                                                                           ggCTGCCCGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCtt                    <  2:2238868/148‑1 (MQ=255)
                                                                                                                                 ccGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAt              <  1:2596281/148‑1 (MQ=255)
                                                                                                                                 ccGGTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAt              <  1:2767993/148‑1 (MQ=255)
                                                                                                                                 ccGGTCATCAACCAGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGACTTTACCGCTTGGCAGGCGAAAACCGGGAACGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACAt              <  2:1641255/148‑1 (MQ=255)
                                                                                                                                   ggTCATCAACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTg            >  2:3447217/1‑148 (MQ=255)
                                                                                                                                     tcatcaACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTc          <  1:1105507/148‑1 (MQ=255)
                                                                                                                                      catcaACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCa         >  1:177631/1‑148 (MQ=255)
                                                                                                                                      catcaACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCa         >  1:2924214/1‑148 (MQ=255)
                                                                                                                                       atcaACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTGCAACATTGTCAc        >  2:2690867/1‑148 (MQ=255)
                                                                                                                                         caACCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACtt      >  1:3775964/1‑148 (MQ=255)
                                                                                                                                           aCCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTc    >  1:1590587/1‑148 (MQ=255)
                                                                                                                                           aCCCGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTc    <  1:1005971/148‑1 (MQ=255)
                                                                                                                                            cccGATTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTCa   <  1:3291014/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTACCCATCTTCTCGAACTGTTGTCCCCAGGTGTCTTCCGTGGTTGCCAGAATCACCGAGGTGAATTTTGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTATCAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCACTTTCAC  >  CP009273/2589789‑2590077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑