Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,614,168 C→A 100% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,614,1680CA99.6% 817.4 / ‑5.9 254D22Y (GAT→TAT) uppuracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (136/113);  minor base T (0/5);  total (136/118)
Fisher's exact test for biased strand distribution p-value = 2.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

TCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG  >  CP009273/2614024‑2614312
                                                                                                                                                |                                                                                                                                                
tCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTACGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGTTATAttt                                                                                                                                               >  1:3752900/1‑147 (MQ=255)
   tACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCACGCTACCCACTTCGGAAGCCAGTTAGCGAAATCGCTTGGTGCTGATATATTtctc                                                                                                                                            >  1:2522200/1‑148 (MQ=255)
    aCCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCa                                                                                                                                           <  2:895522/148‑1 (MQ=255)
        ttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTAGATAGTTACTTTTTACGTTTAGAGGTCGGCGGTCGCTTGGTTAGTCAGCAGGCAACCCACATCGGAACCGCGTTGGCGAAAGCCCGTTGTTCTGATATATTTCTCACCCa                                                                                                                                       >  1:3258667/1‑148 (MQ=255)
        ttGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAAATTTTCCGTTTGGGGGTCGGCGGTCGCGTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTGGCGAAAGCGCTTGGTGGGGATATATTGCTCACGCa                                                                                                                                       >  1:3276493/1‑148 (MQ=255)
         tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAt                                                                                                                                      <  2:3142262/148‑1 (MQ=255)
         tGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAt                                                                                                                                      <  2:2337614/148‑1 (MQ=255)
         tGATCTGGTCGATTTCGACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCAt                                                                                                                                      <  1:1514805/148‑1 (MQ=255)
          gATCTGGTCGATTTCGACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATc                                                                                                                                     <  1:222073/148‑1 (MQ=255)
           aTCTGGTCGATTTCGACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCa                                                                                                                                    <  1:1268508/148‑1 (MQ=255)
            tCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAg                                                                                                                                   <  2:188035/148‑1 (MQ=255)
               ggTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTcc                                                                                                                                >  2:1391805/1‑148 (MQ=255)
                gTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCGTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTccc                                                                                                                               >  1:1562099/1‑148 (MQ=255)
                  cGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAg                                                                                                                             >  1:268575/1‑148 (MQ=255)
                  cGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTAGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAg                                                                                                                             >  1:267185/1‑148 (MQ=255)
                    aTTTCTACCGGGCCGTTCCAGCCGTCGATAGTTAATTTTTCCGTTTAGAGGTCGGCGGTCGCTTCGTAAATAAGCAGGCTAACCACATCGGAAGCGAGTTAGCGAAAACCCGTGGTGGTGATATATTGCTAACCCAAAAATCCCAGcg                                                                                                                           >  1:998573/1‑147 (MQ=255)
                      ttCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTtg                                                                                                                         >  2:181831/1‑148 (MQ=255)
                       tCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCAACCCACATAGGAAGCGAGTTAGCGAAATCGCTTGGTGGTGATATATTTCTCACGCAACAGTCCCAGCTtgt                                                                                                                        >  1:1756898/1‑148 (MQ=255)
                         tACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTgtgt                                                                                                                      <  1:397943/148‑1 (MQ=255)
                         tACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTgtgt                                                                                                                      <  1:3047741/148‑1 (MQ=255)
                            cGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGtttt                                                                                                                   >  2:1626451/1‑147 (MQ=255)
                            cGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTg                                                                                                                   <  1:3024679/148‑1 (MQ=255)
                               gCCGTTCCAGCCTTCGATAGTTATTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACATCGGAAGCGAGTTCGCGAAATCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACg                                                                                                                >  1:1212424/1‑148 (MQ=255)
                                 cGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAg                                                                                                              <  1:1119184/148‑1 (MQ=255)
                                   ttCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTg                                                                                                            >  1:2946744/1‑148 (MQ=255)
                                    tCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTgg                                                                                                           >  2:3591165/1‑148 (MQ=255)
                                     ccAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTggg                                                                                                          >  2:1960670/1‑148 (MQ=255)
                                      cAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgt                                                                                                         <  2:2380222/148‑1 (MQ=255)
                                       aGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGgtg                                                                                                        <  1:921056/148‑1 (MQ=255)
                                          cTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGttt                                                                                                     <  2:3488451/148‑1 (MQ=255)
                                           ttCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTg                                                                                                    <  1:11496/148‑1 (MQ=255)
                                            tCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGa                                                                                                   >  1:2445055/1‑148 (MQ=255)
                                            tCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGa                                                                                                   <  1:1331007/148‑1 (MQ=255)
                                               aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                                >  1:2100709/1‑148 (MQ=255)
                                               aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                                <  2:2705294/148‑1 (MQ=255)
                                               aTAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACtt                                                                                                >  1:569262/1‑148 (MQ=255)
                                                tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGACAGCGCTTGGTGCTGATATATTGCTCACGCATCACTCCCAGCTTGTGTTTGACGTGTGGGTGTTTGACTTc                                                                                               >  2:756639/1‑148 (MQ=255)
                                                tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                               >  1:3256976/1‑148 (MQ=255)
                                                tAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTc                                                                                               >  2:3272768/1‑148 (MQ=255)
                                                 aGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTcc                                                                                              >  2:258098/1‑148 (MQ=255)
                                                  gTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCa                                                                                             <  1:1853177/148‑1 (MQ=255)
                                                     aCTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGa                                                                                          <  2:619540/148‑1 (MQ=255)
                                                      cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                         <  2:1734867/148‑1 (MQ=255)
                                                      cTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGAt                                                                                         >  2:1900683/1‑148 (MQ=255)
                                                       tttttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATc                                                                                        >  1:3443239/1‑148 (MQ=255)
                                                        ttttCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCt                                                                                       <  2:1559563/148‑1 (MQ=255)
                                                           tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCa                                                                                    <  1:3727934/148‑1 (MQ=255)
                                                           tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCa                                                                                    >  2:2458933/1‑148 (MQ=255)
                                                           tCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCa                                                                                    >  2:3356473/1‑148 (MQ=255)
                                                             cGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATa                                                                                  <  2:3435065/148‑1 (MQ=255)
                                                               tttCGAGGTCGGCGGTCGCTTCGTAATTCAGCACGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGGTGATATATTGCTCACGCAACAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAca                                                                                >  1:332938/1‑147 (MQ=255)
                                                               tttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAct                                                                                <  1:2745295/148‑1 (MQ=255)
                                                               tttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATAct                                                                                >  2:125822/1‑148 (MQ=255)
                                                                ttCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATActc                                                                               >  2:3015968/1‑148 (MQ=255)
                                                                         ggcggTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCtt                                                                      <  1:228191/148‑1 (MQ=255)
                                                                              tCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAgg                                                                 >  2:2083949/1‑148 (MQ=255)
                                                                               cGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAggg                                                                >  2:2158164/1‑148 (MQ=255)
                                                                                    cGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGc                                                           <  2:2522200/148‑1 (MQ=255)
                                                                                    cGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGc                                                           <  1:3050348/148‑1 (MQ=255)
                                                                                      tAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCca                                                         >  1:3379877/1‑148 (MQ=255)
                                                                                             gcagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaa                                                  >  2:2034414/1‑148 (MQ=255)
                                                                                              cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 >  2:2843506/1‑148 (MQ=255)
                                                                                              cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 >  2:463457/1‑148 (MQ=255)
                                                                                              cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 >  1:1297066/1‑148 (MQ=255)
                                                                                              cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 >  2:1854181/1‑148 (MQ=255)
                                                                                              cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 >  1:3202226/1‑148 (MQ=255)
                                                                                              cagGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaa                                                 <  1:760865/148‑1 (MQ=255)
                                                                                               agGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaaa                                                <  1:1523566/148‑1 (MQ=255)
                                                                                                ggCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAAt                                               <  2:1951753/148‑1 (MQ=255)
                                                                                                 gCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATc                                              >  2:313521/1‑148 (MQ=255)
                                                                                                   tACCCACTTCGGATGCTAGTTGGCGAATGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGTGTGGGTGTTTTACTTCCACGATCTTCATACTCTTTCTCCTTTTAGGGGCAGCCACAAAAAAAATCGa                                            <  2:1151000/148‑1 (MQ=255)
                                                                                                     cccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCCCAAAAAAAATCGACg                                          >  2:1993546/1‑148 (MQ=255)
                                                                                                      ccACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACgg                                         >  1:3228221/1‑148 (MQ=255)
                                                                                                         cTTCGGAAGCGTGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCTGTCCAAGATTGTTTTTGACTTGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                      <  1:265786/148‑1 (MQ=255)
                                                                                                         cTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                      >  1:1382008/1‑148 (MQ=255)
                                                                                                         cTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                      >  2:2226203/1‑148 (MQ=255)
                                                                                                         cTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGAtt                                      >  1:1608194/1‑148 (MQ=255)
                                                                                                           tCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATtat                                    >  1:441182/1‑148 (MQ=255)
                                                                                                           tCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATtat                                    >  1:440913/1‑148 (MQ=255)
                                                                                                                 gCGAGTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcc                              >  1:1390189/1‑148 (MQ=255)
                                                                                                                  cGAGTTCGCGAAAGCGCGTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATAcctcct                             <  2:1280613/148‑1 (MQ=255)
                                                                                                                    aGTTCGCCAACGGGTTTGGTGCTTATATTTTGCACACGAATCAGTCCCAGGTTTTGTTTGACGTGTGGGTTTTTGACTACCACGATCTTAATACTCTTGCTCCTTTGAGGGGCAGCAACAAAAAAAATCGACGGATTATCCCTCCttt                           <  1:2005103/148‑1 (MQ=255)
                                                                                                                     gTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTTTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTttc                          <  2:3759264/148‑1 (MQ=255)
                                                                                                                     gTTCGCGAAAGCGCTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttc                          >  1:577148/1‑148 (MQ=255)
                                                                                                                       tCGCGAAAGCGCTTGGTGCTTATATATTGCTCACGCATCATTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTttctt                        <  2:1695420/148‑1 (MQ=255)
                                                                                                                               gcgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg                >  1:803335/1‑148 (MQ=255)
                                                                                                                               gcgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcgg                >  2:1021244/1‑148 (MQ=255)
                                                                                                                                cgcTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAggcggc               >  2:3532148/1‑148 (MQ=255)
                                                                                                                                  cTTGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCaa             >  1:170203/1‑148 (MQ=255)
                                                                                                                                    tGGTGCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAata           >  1:2735840/1‑148 (MQ=255)
                                                                                                                                     ggTGCTTATATATTGCTCACGAATCAGTCCCAGCTTGTTTTTGACGTGTGGGTTTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAatat          <  1:3356473/148‑1 (MQ=255)
                                                                                                                                       tgtttttatTTTGCTCACGCTTCAGTCCCAGTTTGTTTTTGTCGTTTGGGTGTTTTACTTCCAGTATATTCATACTTTTTCTCATTTGAGGTTAAGCAAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTc        <  1:2757287/142‑1 (MQ=255)
                                                                                                                                        gCTGATATTTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt       <  2:3256976/148‑1 (MQ=255)
                                                                                                                                        gCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt       >  2:259652/1‑148 (MQ=255)
                                                                                                                                        gCTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt       <  2:3735922/148‑1 (MQ=255)
                                                                                                                                         gttatatatTGCTCACGATTCAGTCCCAGTTTGTGTTTGACGTTTGGGTTTTTGACTACCCCTATCTTCATACTGTTTCTCCTTTGAGGGGCACCCAAAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt      <  1:2573476/145‑1 (MQ=255)
                                                                                                                                         cTGATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt      <  2:1162518/148‑1 (MQ=255)
                                                                                                                                           gATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtttt    <  2:391464/148‑1 (MQ=255)
                                                                                                                                           gATATATTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtttt    <  2:1908241/148‑1 (MQ=255)
                                                                                                                                             tataTTGCTCACGCATCTGGCCCAGCTTGTTTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTGGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCg  <  1:3099842/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCTTACCTTTGATCTGGTCGATTTCTACCGGGCCGTTCCAGCCTTCGATAGTTACTTTTTCCGTTTCGAGGTCGGCGGTCGCTTCGTAAGTCAGCAGGCTACCCACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCG  >  CP009273/2614024‑2614312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑