Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,618,693 G→T 100% E74* (GAA→TAA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,618,6930GT98.6% 706.4 / 0.5 220E74* (GAA→TAA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (108/109);  total (110/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAG  >  CP009273/2618548‑2618837
                                                                                                                                                 |                                                                                                                                                
ggTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTaa                                                                                                                                                <  1:1036662/148‑1 (MQ=255)
   cATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACgg                                                                                                                                             <  2:3659369/148‑1 (MQ=255)
    aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTNGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGc                                                                                                                                            <  1:1936747/148‑1 (MQ=255)
    aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGc                                                                                                                                            <  1:3325474/148‑1 (MQ=255)
     tAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCt                                                                                                                                           >  2:2187476/1‑148 (MQ=255)
      aGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTa                                                                                                                                          >  2:816782/1‑148 (MQ=255)
       gCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTAc                                                                                                                                         >  1:3794370/1‑148 (MQ=255)
        cccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACa                                                                                                                                        >  1:1101587/1‑148 (MQ=255)
        cccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACa                                                                                                                                        >  1:776986/1‑148 (MQ=255)
         ccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACaa                                                                                                                                       <  1:692681/148‑1 (MQ=255)
          cGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAg                                                                                                                                      >  2:3538185/1‑148 (MQ=255)
           gtgtGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAgg                                                                                                                                     <  2:308354/148‑1 (MQ=255)
              tgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGtt                                                                                                                                  <  1:467878/148‑1 (MQ=255)
                  aGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTtct                                                                                                                              <  1:3491249/148‑1 (MQ=255)
                    aTGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTcc                                                                                                                            >  1:1466035/1‑148 (MQ=255)
                      ggTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTg                                                                                                                          >  1:3483267/1‑148 (MQ=255)
                        tGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGcc                                                                                                                        >  2:2254729/1‑148 (MQ=255)
                        tGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGcc                                                                                                                        >  2:2254013/1‑148 (MQ=255)
                             tGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGt                                                                                                                   <  1:267930/148‑1 (MQ=255)
                              gCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                  >  1:1858751/1‑148 (MQ=255)
                              gCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                  <  1:876720/148‑1 (MQ=255)
                              gCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                  <  1:876555/148‑1 (MQ=255)
                              gCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                  >  1:3008049/1‑148 (MQ=255)
                              gCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                  >  1:1256144/1‑148 (MQ=255)
                                aGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTg                                                                                                                >  2:2391720/1‑148 (MQ=255)
                                 gATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGt                                                                                                               >  1:2386256/1‑148 (MQ=255)
                                   ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                             <  1:2397455/148‑1 (MQ=255)
                                   ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                             <  1:2397970/148‑1 (MQ=255)
                                   ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                             >  1:630575/1‑148 (MQ=255)
                                   ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                             >  1:3357066/1‑148 (MQ=255)
                                     attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAg                                                                                                           <  2:1285944/148‑1 (MQ=255)
                                     attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAg                                                                                                           <  2:195230/148‑1 (MQ=255)
                                     attGGCCGCCGGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGTCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAg                                                                                                           >  2:2738902/1‑148 (MQ=255)
                                      ttGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGt                                                                                                          >  1:3120376/1‑148 (MQ=255)
                                           cgccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTa                                                                                                     <  2:1106290/148‑1 (MQ=255)
                                             ccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAcc                                                                                                   >  1:1124328/1‑148 (MQ=255)
                                                gAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCAt                                                                                                <  1:1523049/148‑1 (MQ=255)
                                                 aaaCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATa                                                                                               <  2:2715394/148‑1 (MQ=255)
                                                   aCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACg                                                                                             <  2:3055237/148‑1 (MQ=255)
                                                    cAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGc                                                                                            >  2:160857/1‑148 (MQ=255)
                                                    cAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGc                                                                                            >  1:3539067/1‑148 (MQ=255)
                                                      gCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTg                                                                                          <  1:2444880/148‑1 (MQ=255)
                                                         ggTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGt                                                                                       <  1:2066526/148‑1 (MQ=255)
                                                         ggTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGt                                                                                       >  1:3780844/1‑148 (MQ=255)
                                                               tCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgcg                                                                                 <  1:2858708/148‑1 (MQ=255)
                                                                  ggCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg                                                                              >  1:3573318/1‑148 (MQ=255)
                                                                  ggCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTg                                                                              <  1:2599453/148‑1 (MQ=255)
                                                                    cGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGaa                                                                            <  2:2636994/148‑1 (MQ=255)
                                                                          gCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCAc                                                                      >  2:355828/1‑148 (MQ=255)
                                                                            cTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTg                                                                    <  2:2242298/148‑1 (MQ=255)
                                                                              ggggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAc                                                                  >  1:3745025/1‑148 (MQ=255)
                                                                              ggggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAc                                                                  >  1:486992/1‑148 (MQ=255)
                                                                                  ccAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTc                                                              <  2:2486389/148‑1 (MQ=255)
                                                                                     gATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGa                                                           >  1:475200/1‑148 (MQ=255)
                                                                                      ataataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGaa                                                          >  1:2383976/1‑148 (MQ=255)
                                                                                         atatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcg                                                       <  2:3208378/148‑1 (MQ=255)
                                                                                         atatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcg                                                       <  1:1496906/148‑1 (MQ=255)
                                                                                         atatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcg                                                       <  2:1300893/148‑1 (MQ=255)
                                                                                          tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgc                                                      >  1:718865/1‑148 (MQ=255)
                                                                                           atGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcg                                                     >  1:1948904/1‑148 (MQ=255)
                                                                                           atGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcg                                                     >  1:415528/1‑148 (MQ=255)
                                                                                            tGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgc                                                    >  1:2901833/1‑148 (MQ=255)
                                                                                            tGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgc                                                    <  2:2114786/148‑1 (MQ=255)
                                                                                               tGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGa                                                 >  2:776086/1‑148 (MQ=255)
                                                                                                 aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaa                                               >  1:1493316/1‑148 (MQ=255)
                                                                                                 aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaa                                               <  1:243460/148‑1 (MQ=255)
                                                                                                   tGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAg                                             <  2:160928/148‑1 (MQ=255)
                                                                                                     aaGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGt                                           >  1:100666/1‑148 (MQ=255)
                                                                                                      agagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTc                                          <  1:3283298/148‑1 (MQ=255)
                                                                                                      agagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTc                                          >  2:2009443/1‑148 (MQ=255)
                                                                                                        agGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGACACGCGCGGAAAAGGTCAt                                        >  2:157039/1‑148 (MQ=255)
                                                                                                        agGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAt                                        >  1:2778416/1‑148 (MQ=255)
                                                                                                          gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTc                                      <  2:3222118/148‑1 (MQ=255)
                                                                                                                aCGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTAcc                                <  2:1597694/148‑1 (MQ=255)
                                                                                                                  tcgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGTTTTTCTACTTACAGCGTCTGTATATTTGGTACCCATACGCTGCGTCAGGCGCTGAACTCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCg                              <  1:2151019/147‑1 (MQ=255)
                                                                                                                  gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCg                              <  2:3381818/148‑1 (MQ=255)
                                                                                                                   cgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGACAAGGTCATTGCCTACCCGa                             >  2:2032194/1‑148 (MQ=255)
                                                                                                                   cgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGa                             <  1:2036910/148‑1 (MQ=255)
                                                                                                                         ttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaaa                       <  2:424982/148‑1 (MQ=255)
                                                                                                                         ttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaaa                       >  1:2012126/1‑148 (MQ=255)
                                                                                                                         ttAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaaa                       <  2:2870100/148‑1 (MQ=255)
                                                                                                                           aaaCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatt                     <  2:3425273/148‑1 (MQ=255)
                                                                                                                           aaaCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatt                     <  2:105906/148‑1 (MQ=255)
                                                                                                                             aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                   >  2:1475760/1‑148 (MQ=255)
                                                                                                                             aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                   <  2:2386189/148‑1 (MQ=255)
                                                                                                                             aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                   >  2:197497/1‑148 (MQ=255)
                                                                                                                             aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                   >  2:1709089/1‑148 (MQ=255)
                                                                                                                              ctgtctgtCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattatt                  <  2:2939846/148‑1 (MQ=255)
                                                                                                                              ctgtctgtCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattatt                  >  1:2294044/1‑148 (MQ=255)
                                                                                                                               tgtctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTAttt                 <  2:3165305/148‑1 (MQ=255)
                                                                                                                                 tctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTcc               <  2:3794370/148‑1 (MQ=255)
                                                                                                                                  ctgtcGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAACCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCg              >  2:405690/1‑148 (MQ=255)
                                                                                                                                   tgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCgg             >  2:640043/1‑148 (MQ=255)
                                                                                                                                        cTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATg        >  1:3305629/1‑148 (MQ=255)
                                                                                                                                         tGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATga       <  1:3795671/148‑1 (MQ=255)
                                                                                                                                          gTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaa      >  1:2168518/1‑148 (MQ=255)
                                                                                                                                           tttGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaag     >  2:2061951/1‑148 (MQ=255)
                                                                                                                                            ttGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaaga    <  1:3393492/148‑1 (MQ=255)
                                                                                                                                              gCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGaagaag  <  1:197497/148‑1 (MQ=255)
                                                                                                                                              gCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGaagaag  <  2:725365/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAG  >  CP009273/2618548‑2618837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑