BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,633,402	C→A	94.7%	R178L (CGC→CTC)	hisS ←	histidyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,633,402	0	C	A	94.7%	511.4 / 15.9	179	R178L (CGC→CTC)	hisS	histidyl tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (5/5); new base A (80/89); total (85/94)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

ACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTC  >  CP009273/2633257‑2633544

aCCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAgg                                                                                                                                              <  1:3532936/148‑1 (MQ=255)

aCCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAgg                                                                                                                                              <  1:1640108/148‑1 (MQ=255)

 ccTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGt                                                                                                                                             >  1:564261/1‑148 (MQ=255)

 ccTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGt                                                                                                                                             <  1:2429657/148‑1 (MQ=255)

  cTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTa                                                                                                                                            >  2:833101/1‑148 (MQ=255)

    aaTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAt                                                                                                                                          <  2:3810174/148‑1 (MQ=255)

     aTGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAAtt                                                                                                                                         <  2:513460/148‑1 (MQ=255)

           gAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGcgcg                                                                                                                                   >  2:2507237/1‑148 (MQ=255)

           gAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTAGTCCAGCTTTTCTTTATGCTGCTCAAGGAATTCCACCAGCGCATCGAGGTAAATGGcgcg                                                                                                                                   >  1:1720492/1‑148 (MQ=255)

            aGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCCTCGAGGTAATTGGCGCGt                                                                                                                                  >  2:2366590/1‑148 (MQ=255)

            aGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGt                                                                                                                                  >  2:3116783/1‑148 (MQ=255)

            aGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATACAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTATTCGTACAGCTTTTATTTATTCTTCTCAATGAATTCCACCAGCGCATCCAGGTAATTGGCGCGt                                                                                                                                  >  1:2961738/1‑148 (MQ=255)

                  gtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTcc                                                                                                                            <  1:410870/148‑1 (MQ=255)

                  gtTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTcc                                                                                                                            <  1:1174725/148‑1 (MQ=255)

                   ttGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCa                                                                                                                           >  2:2264177/1‑148 (MQ=255)

                    tGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAg                                                                                                                          >  2:953441/1‑148 (MQ=255)

                    tGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAg                                                                                                                          >  1:1940675/1‑148 (MQ=255)

                      agaAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTCATTGGCGCGTGCTTCCcgcg                                                                                                                        >  1:3361912/1‑148 (MQ=255)

                       gaAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCCTCGAGGTAATTGGCGCGTGCTTCCAGCGa                                                                                                                       >  1:2304918/1‑148 (MQ=255)

                       gaAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGa                                                                                                                       <  1:3461116/148‑1 (MQ=255)

                        aaGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTTAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGaa                                                                                                                      <  2:3069960/148‑1 (MQ=255)

                           cgcCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCg                                                                                                                   >  2:661508/1‑148 (MQ=255)

                               tGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATag                                                                                                               <  1:1283628/148‑1 (MQ=255)

                               tGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATag                                                                                                               >  1:1220053/1‑148 (MQ=255)

                                   cTTCCTGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                           <  2:2067383/148‑1 (MQ=255)

                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                           >  2:3206566/1‑148 (MQ=255)

                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                           >  1:2423571/1‑148 (MQ=255)

                                   cTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGtt                                                                                                           <  2:1693640/148‑1 (MQ=255)

                                    ttCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTc                                                                                                          <  2:2168062/148‑1 (MQ=255)

                                     tCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCa                                                                                                         >  2:1275694/1‑148 (MQ=255)

                                            tttttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAag                                                                                                  <  2:1655671/148‑1 (MQ=255)

                                            tttttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAag                                                                                                  <  2:999025/148‑1 (MQ=255)

                                               ttGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGt                                                                                               <  1:126724/148‑1 (MQ=255)

                                                 gAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTa                                                                                             <  1:1978887/148‑1 (MQ=255)

                                                   aTCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACg                                                                                           <  2:2472611/148‑1 (MQ=255)

                                                   aTCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACg                                                                                           >  2:791645/1‑148 (MQ=255)

                                                     ccAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTg                                                                                         >  2:1783037/1‑148 (MQ=255)

                                                     ccAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTg                                                                                         >  1:2443939/1‑148 (MQ=255)

                                                        gCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTc                                                                                      >  1:3130311/1‑148 (MQ=255)

                                                         cACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCg                                                                                     <  2:257741/148‑1 (MQ=255)

                                                         cACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCg                                                                                     <  1:1316966/148‑1 (MQ=255)

                                                         cACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCg                                                                                     >  1:2942131/1‑148 (MQ=255)

                                                              gcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAAt                                                                                <  1:2998001/148‑1 (MQ=255)

                                                              gcAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAAt                                                                                <  2:1064444/148‑1 (MQ=255)

                                                                 gCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAcc                                                                             >  1:495183/1‑148 (MQ=255)

                                                                 gCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATAcc                                                                             <  1:1268435/148‑1 (MQ=255)

                                                                  cGGCTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCATTTCTTTATGCTGCTCAAGGACAGCCACCACCGCAACGAGGTAATTGGCGCGTGCTTCCAGCGAACCGAAAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAAAAccc                                                                            >  2:3649613/1‑148 (MQ=255)

                                                                          tgtACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAgcgcgcg                                                                    >  2:2698903/1‑148 (MQ=255)

                                                                                tGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGccacca                                                              >  2:3125797/1‑148 (MQ=255)

                                                                                 gcggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAg                                                             <  1:2841898/148‑1 (MQ=255)

                                                                                   ggcgTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCg                                                           <  2:3760062/148‑1 (MQ=255)

                                                                                      gTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGc                                                        <  2:3132824/148‑1 (MQ=255)

                                                                                       tttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGTCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCa                                                       <  1:2790632/148‑1 (MQ=255)

                                                                                        ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                      <  2:399113/148‑1 (MQ=255)

                                                                                        ttGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAg                                                      <  2:399808/148‑1 (MQ=255)

                                                                                         tGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGt                                                     <  2:3359798/148‑1 (MQ=255)

                                                                                           cAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGa                                                   >  1:80910/1‑148 (MQ=255)

                                                                                           cAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGa                                                   >  1:79961/1‑148 (MQ=255)

                                                                                              tCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCa                                                <  2:2887388/148‑1 (MQ=255)

                                                                                                ttCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATa                                              >  2:3071866/1‑148 (MQ=255)

                                                                                                 tCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATaa                                             >  1:1333732/1‑148 (MQ=255)

                                                                                                  cGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAAt                                            <  1:835091/148‑1 (MQ=255)

                                                                                                    tCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCa                                          <  1:2443271/148‑1 (MQ=255)

                                                                                                     ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAg                                         >  1:984920/1‑148 (MQ=255)

                                                                                                     ccAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCAACGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGAGCGCCACCAGCGGGCAGTGAGCATAATCAg                                         >  2:3309844/1‑148 (MQ=255)

                                                                                                       aGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGtt                                       >  2:1427252/1‑148 (MQ=255)

                                                                                                       aGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGtt                                       <  1:2476833/148‑1 (MQ=255)

                                                                                                        gCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTc                                      >  1:1877509/1‑148 (MQ=255)

                                                                                                         cTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCa                                     >  1:1419572/1‑148 (MQ=255)

                                                                                                           tttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGc                                   >  2:3531715/1‑148 (MQ=255)

                                                                                                            ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                  >  1:460675/1‑148 (MQ=255)

                                                                                                            ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                  >  1:1341410/1‑148 (MQ=255)

                                                                                                            ttCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCg                                  <  2:841833/148‑1 (MQ=255)

                                                                                                             tCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGt                                 <  2:1124304/148‑1 (MQ=255)

                                                                                                              cTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTc                                >  1:3319329/1‑148 (MQ=255)

                                                                                                              cTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTc                                >  1:313497/1‑148 (MQ=255)

                                                                                                               tttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCg                               <  2:2086482/148‑1 (MQ=255)

                                                                                                                ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                              >  2:1159586/1‑148 (MQ=255)

                                                                                                                ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                              >  2:2199117/1‑148 (MQ=255)

                                                                                                                ttATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGa                              >  2:320552/1‑148 (MQ=255)

                                                                                                                 tATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                             <  2:3509795/148‑1 (MQ=255)

                                                                                                                 tATGCTGCTCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGat                             <  2:197703/148‑1 (MQ=255)

                                                                                                                   tgctgcTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                           <  1:1088259/148‑1 (MQ=255)

                                                                                                                   tgctgcTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGatat                           <  1:1783037/148‑1 (MQ=255)

                                                                                                                    gctgctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATc                          <  1:3552082/148‑1 (MQ=255)

                                                                                                                     ctgctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATcc                         >  1:812919/1‑148 (MQ=255)

                                                                                                                        ctCAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGa                      <  1:480357/148‑1 (MQ=255)

                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                    >  1:2967614/1‑148 (MQ=255)

                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                    >  1:24066/1‑148 (MQ=255)

                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                    <  1:2044743/148‑1 (MQ=255)

                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                    >  2:576508/1‑148 (MQ=255)

                                                                                                                          cAAGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGAcc                    <  1:498166/148‑1 (MQ=255)

                                                                                                                            aGGAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCtt                  >  2:154026/1‑148 (MQ=255)

                                                                                                                             ggAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTg                 >  1:1004328/1‑148 (MQ=255)

                                                                                                                              gAATGCCACCAGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGc                >  2:2452601/1‑148 (MQ=255)

                                                                                                                                      ccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaa        >  1:198852/1‑148 (MQ=255)

                                                                                                                                      ccaGCGCATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGaa        >  2:1337633/1‑148 (MQ=255)

                                                                                                                                         gcgcATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAAc     <  1:3043557/148‑1 (MQ=255)

                                                                                                                                          cgcATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACt    <  2:491397/148‑1 (MQ=255)

                                                                                                                                            cATCGAGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGGTCAGCGCAAGAGTTAAGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACtta  >  2:1490180/1‑147 (MQ=255)

ACCTAATGCCGGAGCGTCGTTGAGAAGCGCCTGCACTTCCGGATTTTTTGAATCCAGCACGCGCAGCGGGTTAGTGTACATGCGGCGTTTGCAGTCTTCGTCCAGCTTTTCTTTATGCTGCTCAAGGAATGCCACCAGCGCATCGCGGTAATTGGCGCGTGCTTCCAGCGAACCGATAGAGTTCAGCTCAAGAGTTACGTGCTCGGAAATACCCAGCGCGCGCCACCAGCGGGCAGTGAGCATAATCAGTTCAGCGTCGATATCCGGACCTTGCAGACCGAAAACTTC  >  CP009273/2633257‑2633544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`