Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,669,978 C→A 100% A69A (GCG→GCT)  yphD ← inner membrane putative ABC superfamily sugar transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,669,9780CA98.4% 652.6 / 0.7 207A69A (GCG→GCT) yphDinner membrane putative ABC superfamily sugar transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (116/87);  total (116/91)
Fisher's exact test for biased strand distribution p-value = 3.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

TCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGA  >  CP009273/2669833‑2670122
                                                                                                                                                 |                                                                                                                                                
 cAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGcc                                                                                                                                               >  2:2842865/1‑148 (MQ=255)
 cAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGcc                                                                                                                                               >  2:735397/1‑148 (MQ=255)
   gcgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCa                                                                                                                                             <  1:1854880/148‑1 (MQ=255)
    cgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAg                                                                                                                                            <  1:1094804/148‑1 (MQ=255)
    cgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAg                                                                                                                                            >  1:2926113/1‑148 (MQ=255)
        ccTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAggcgg                                                                                                                                        <  1:74342/148‑1 (MQ=255)
           aaCAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCaa                                                                                                                                     <  2:3230818/148‑1 (MQ=255)
               gCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATccc                                                                                                                                 >  2:3686761/1‑148 (MQ=255)
                cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCTTCATAGACAAGGCGGCAATCCCa                                                                                                                                >  2:2755223/1‑148 (MQ=255)
                cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCa                                                                                                                                >  1:3462274/1‑148 (MQ=255)
                cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCa                                                                                                                                <  1:549509/148‑1 (MQ=255)
                cAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCa                                                                                                                                <  2:1589956/148‑1 (MQ=255)
                 aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCaa                                                                                                                               <  2:3337801/148‑1 (MQ=255)
                 aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCaa                                                                                                                               <  2:3334460/148‑1 (MQ=255)
                 aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCaa                                                                                                                               <  2:3087945/148‑1 (MQ=255)
                 aaCACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCaa                                                                                                                               <  2:1833950/148‑1 (MQ=255)
                  acacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                              >  1:324812/1‑148 (MQ=255)
                  acacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAt                                                                                                                              >  1:3262937/1‑148 (MQ=255)
                   cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                             <  2:219515/148‑1 (MQ=255)
                   cacCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtg                                                                                                                             >  1:761461/1‑148 (MQ=255)
                     ccAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAAtggt                                                                                                                           <  1:1610853/148‑1 (MQ=255)
                            gCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCa                                                                                                                    >  2:1089/1‑148 (MQ=255)
                              aCGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATc                                                                                                                  >  1:1565738/1‑148 (MQ=255)
                                  cACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCCTCGGCCCAACGCTGACATCAATTTCACCGGAGAGAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCgcgc                                                                                                              <  2:145051/148‑1 (MQ=255)
                                   aCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgca                                                                                                             >  1:1155829/1‑148 (MQ=255)
                                   aCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgca                                                                                                             >  1:2005405/1‑148 (MQ=255)
                                   aCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgca                                                                                                             >  1:1276862/1‑148 (MQ=255)
                                    ccGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcag                                                                                                            >  1:887571/1‑148 (MQ=255)
                                    ccGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcag                                                                                                            >  1:2210179/1‑148 (MQ=255)
                                             gAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                                                                   <  1:1675227/148‑1 (MQ=255)
                                              aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATc                                                                                                  <  1:944845/148‑1 (MQ=255)
                                              aaCTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATc                                                                                                  >  1:2780957/1‑148 (MQ=255)
                                                cTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCCGCACGTTCATCTg                                                                                                >  1:804697/1‑148 (MQ=255)
                                                  tCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                              >  2:2654971/1‑148 (MQ=255)
                                                  tCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGt                                                                                              >  1:581633/1‑148 (MQ=255)
                                                     aaTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTAt                                                                                           >  2:2417627/1‑148 (MQ=255)
                                                     aaTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTAt                                                                                           >  2:1928124/1‑148 (MQ=255)
                                                      aTTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTAtt                                                                                          >  1:2665142/1‑148 (MQ=255)
                                                        tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCa                                                                                        >  2:1695227/1‑148 (MQ=255)
                                                        tGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCa                                                                                        >  1:709141/1‑148 (MQ=255)
                                                         gcagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                       <  2:3802944/148‑1 (MQ=255)
                                                         gcagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCaa                                                                                       <  2:1913898/148‑1 (MQ=255)
                                                          cagcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAAt                                                                                      >  2:2343774/1‑148 (MQ=255)
                                                           agcaAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATg                                                                                     <  1:3173773/148‑1 (MQ=255)
                                                             caAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATgag                                                                                   <  2:2160225/148‑1 (MQ=255)
                                                                aaaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATa                                                                                >  2:955392/1‑148 (MQ=255)
                                                                 aaTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaa                                                                               >  2:3527204/1‑148 (MQ=255)
                                                                  aTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaaa                                                                              >  1:3586128/1‑148 (MQ=255)
                                                                  aTGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATaaa                                                                              >  1:1051365/1‑148 (MQ=255)
                                                                   tGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAg                                                                             <  1:2929499/148‑1 (MQ=255)
                                                                    gCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGc                                                                            <  1:3456082/148‑1 (MQ=255)
                                                                      cAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCa                                                                          <  2:1748934/148‑1 (MQ=255)
                                                                           acacCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGgcgc                                                                     >  2:621207/1‑148 (MQ=255)
                                                                            cacCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGcc                                                                           <  2:33538/141‑1 (MQ=255)
                                                                            cacCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGcc                                                                           >  1:33538/1‑141 (MQ=255)
                                                                            cacCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGcgcg                                                                    >  2:1304082/1‑148 (MQ=255)
                                                                             acCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGt                                                                   >  1:1508083/1‑148 (MQ=255)
                                                                              ccGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGtt                                                                  <  1:98485/148‑1 (MQ=255)
                                                                                 agaCAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAg                                                               <  2:2474357/148‑1 (MQ=255)
                                                                                     aaaaGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGgag                                                           <  2:443633/148‑1 (MQ=255)
                                                                                         gCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaaga                                                       <  2:157470/148‑1 (MQ=255)
                                                                                                    cccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGa                                            <  1:2504456/148‑1 (MQ=255)
                                                                                                     ccAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGaa                                           <  2:3112522/148‑1 (MQ=255)
                                                                                                      cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                          >  1:297537/1‑148 (MQ=255)
                                                                                                      cAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAAt                                          <  1:1741297/148‑1 (MQ=255)
                                                                                                       aaCGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATg                                         <  1:2061662/148‑1 (MQ=255)
                                                                                                         cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                       >  1:3503837/1‑148 (MQ=255)
                                                                                                         cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                       >  2:348798/1‑148 (MQ=255)
                                                                                                         cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                       >  2:233463/1‑148 (MQ=255)
                                                                                                         cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                       >  2:233479/1‑148 (MQ=255)
                                                                                                         cGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGc                                       >  2:3548225/1‑148 (MQ=255)
                                                                                                          gCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCa                                      <  1:1172629/148‑1 (MQ=255)
                                                                                                             gACATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATc                                   >  1:1857601/1‑148 (MQ=255)
                                                                                                              aCATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATTGTGGCGGCATCGCGCAGCACGATCATCTGGATATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGAAATca                                  >  2:2579052/1‑148 (MQ=255)
                                                                                                              aCATCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATca                                  <  1:3750279/148‑1 (MQ=255)
                                                                                                                 tCAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGgaga                                                          >  2:3419169/1‑121 (MQ=255)
                                                                                                                  cAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcaccac                              >  2:416945/1‑148 (MQ=255)
                                                                                                                  cAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcaccac                              >  2:1141649/1‑148 (MQ=255)
                                                                                                                  cAATTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATcaccac                              <  2:2461356/148‑1 (MQ=255)
                                                                                                                   aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCaccacc                             >  1:3638589/1‑148 (MQ=255)
                                                                                                                   aaTTTCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCaccacc                             <  2:3610687/148‑1 (MQ=255)
                                                                                                                      ttCACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGc                          <  1:2453426/148‑1 (MQ=255)
                                                                                                                        cACCGGAGATAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCaa                        <  1:466271/148‑1 (MQ=255)
                                                                                                                              agaTAATAATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAt                  <  1:1254046/148‑1 (MQ=255)
                                                                                                                                ataataATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtct                >  1:520584/1‑148 (MQ=255)
                                                                                                                                ataataATCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtct                >  1:2440461/1‑148 (MQ=255)
                                                                                                                                 taataaTCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGAtctc               >  1:3274303/1‑148 (MQ=255)
                                                                                                                                   ataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCAt             >  1:2593581/1‑148 (MQ=255)
                                                                                                                                   ataatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCAt             >  2:684452/1‑148 (MQ=255)
                                                                                                                                     aatCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTa           >  1:2106607/1‑148 (MQ=255)
                                                                                                                                      atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa          >  1:3444100/1‑148 (MQ=255)
                                                                                                                                      atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa          <  2:1276846/148‑1 (MQ=255)
                                                                                                                                      atCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTaa          >  1:3653556/1‑148 (MQ=255)
                                                                                                                                       tCAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAat         <  2:3056102/148‑1 (MQ=255)
                                                                                                                                        cAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAACGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAata        <  2:825084/148‑1 (MQ=255)
                                                                                                                                        cAGCGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAata        <  1:2314126/148‑1 (MQ=255)
                                                                                                                                           cGTCATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATgg     >  2:1992302/1‑148 (MQ=255)
                                                                                                                                              cATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGa  >  1:3046996/1‑148 (MQ=255)
                                                                                                                                              cATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGa  >  2:128282/1‑148 (MQ=255)
                                                                                                                                              cATAGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGa  >  1:3046948/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGATAAAGAATGGCAATCACCACCAGCAAACCGATCTCATTAATATGGCGA  >  CP009273/2669833‑2670122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑