Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,689,267 C→A 100% I36I (ATC→ATA)  mltF → membrane‑bound lytic transglycosylase F, murein hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,689,2670CA97.0% 831.5 / 13.8 270I36I (ATC→ATA) mltFmembrane‑bound lytic transglycosylase F, murein hydrolase
Reads supporting (aligned to +/- strand):  ref base C (2/6);  new base A (145/117);  total (147/123)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 275 total aligned reads displayed.

TCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGT  >  CP009273/2689123‑2689410
                                                                                                                                                |                                                                                                                                               
tCAAGCTGGGACGCGCACGACACGGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATagcc                                                                                                                                              >  2:3568806/1‑148 (MQ=255)
 cAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATagccg                                                                                                                                             >  1:2844653/1‑148 (MQ=255)
     cTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAt                                                                                                                                         >  2:2099837/1‑148 (MQ=255)
     cTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCAt                                                                                                                                         >  2:3150873/1‑148 (MQ=255)
        ggACGCGCCCGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTACTTATCTGGTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCa                                                                                                                                      <  1:256044/148‑1 (MQ=255)
        ggACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCa                                                                                                                                      >  2:2516059/1‑148 (MQ=255)
          aCGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAg                                                                                                                                    <  2:1122559/148‑1 (MQ=255)
          aCGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAg                                                                                                                                    <  2:1340609/148‑1 (MQ=255)
          aCGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAg                                                                                                                                    <  1:1873562/148‑1 (MQ=255)
            gcgcACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAgcg                                                                                                                                  >  2:2034790/1‑148 (MQ=255)
               cACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTACCTGGTTTTGTAAAGACGACAACCGTATAGCCGCCATTCACGCGCgg                                                                                                                               >  2:2897145/1‑148 (MQ=255)
                  gACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGga                                                                                                                            <  1:3229106/148‑1 (MQ=255)
                     aaagagAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGagag                                                                                                                         <  2:2941831/146‑1 (MQ=255)
                      caGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGt                                                                                                                        <  1:149385/148‑1 (MQ=255)
                       agagAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGtt                                                                                                                       >  2:1237126/1‑148 (MQ=255)
                       agagAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGtt                                                                                                                       >  1:1480090/1‑148 (MQ=255)
                         agaATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGc                                                                                                                     >  2:1830686/1‑148 (MQ=255)
                              tAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGtgtg                                                                                                                >  2:3364756/1‑148 (MQ=255)
                                aCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtg                                                                                                                  <  1:1108239/144‑1 (MQ=255)
                                aCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCgtg                                                                                                                  <  1:59363/144‑1 (MQ=255)
                                aCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAg                                                                                                              >  2:1602115/1‑148 (MQ=255)
                                aCTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAg                                                                                                              >  2:3800222/1‑148 (MQ=255)
                                 cTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGc                                                                                                             >  1:243246/1‑148 (MQ=255)
                                   aaTTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCAc                                                                                                           >  1:1018772/1‑148 (MQ=255)
                                     ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCCTCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                         >  1:1967487/1‑148 (MQ=255)
                                     ttGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCa                                                                                                         <  2:1251939/148‑1 (MQ=255)
                                      tGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCAt                                                                                                        >  2:2185506/1‑148 (MQ=255)
                                        aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTc                                                                                                      <  1:417738/148‑1 (MQ=255)
                                        aaaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATACAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTc                                                                                                      <  2:1853934/148‑1 (MQ=255)
                                         aaaaaTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTTCGTGTGAGCACCATTCa                                                                                                     >  1:885774/1‑148 (MQ=255)
                                             aTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACt                                                                                                 >  1:849064/1‑148 (MQ=255)
                                             aTTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACt                                                                                                 >  1:2344795/1‑148 (MQ=255)
                                              ttAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTc                                                                                                >  1:2640285/1‑148 (MQ=255)
                                              ttAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTc                                                                                                >  1:3485447/1‑148 (MQ=255)
                                                 aaGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTcccc                                                                                             <  1:3631319/148‑1 (MQ=255)
                                                   gATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTg                                                                                           >  1:2831198/1‑148 (MQ=255)
                                                     ttaattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGAc                                                                                         >  1:1589423/1‑148 (MQ=255)
                                                      taattaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACt                                                                                        >  1:260688/1‑148 (MQ=255)
                                                        attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCAATCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta                                                                                      >  1:778742/1‑148 (MQ=255)
                                                        attaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCAACCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCCATCAATCCCGGGGCGAGTTGCGTGTGAGCACCAATCATACTCCCCTGACTta                                                                                      >  1:971064/1‑148 (MQ=255)
                                                        attaTCTGTTCATCGGCATTCTGGCACTGATGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTta                                                                                      >  2:2866392/1‑148 (MQ=255)
                                                         ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                     >  2:639651/1‑148 (MQ=255)
                                                         ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                     >  2:1709090/1‑148 (MQ=255)
                                                         ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                     >  2:921949/1‑148 (MQ=255)
                                                         ttaTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtat                                                                                     >  1:533649/1‑148 (MQ=255)
                                                          taTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTtata                                                                                    >  1:2736419/1‑148 (MQ=255)
                                                          taTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCAATCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCAATCATACTCCCCTGACTtata                                                                                    >  1:670228/1‑148 (MQ=255)
                                                           atatGTTCATCGGCATTCTGGCACTGCTGCTCGAGGTCGCTCTCTGGCCATCAATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTTCGTGTGAGCACCATTCATACTCCCCTGACTTATaa                                                                                   <  2:2699815/145‑1 (MQ=255)
                                                           aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATaa                                                                                   <  1:767206/148‑1 (MQ=255)
                                                           aTCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATaa                                                                                   >  1:315475/1‑148 (MQ=255)
                                                             cTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCAATCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACg                                                                                 >  1:1384206/1‑148 (MQ=255)
                                                              tGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGa                                                                                <  1:2429889/148‑1 (MQ=255)
                                                              tGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGa                                                                                <  1:2430035/148‑1 (MQ=255)
                                                               gTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAATCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGaa                                                                               >  2:3033842/1‑148 (MQ=255)
                                                                   aTCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGGATAGCCGCCAATCAAGCGCGTGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAAACa                                                                           >  1:3582253/1‑148 (MQ=255)
                                                                    tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCCTCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGACATCaa                                                                          >  2:2599433/1‑148 (MQ=255)
                                                                    tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                          <  2:575787/148‑1 (MQ=255)
                                                                    tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                          <  2:2494058/148‑1 (MQ=255)
                                                                    tCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCaa                                                                          >  2:3246174/1‑148 (MQ=255)
                                                                       gCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACgg                                                                       >  1:1600447/1‑148 (MQ=255)
                                                                        cATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                      <  1:3352341/148‑1 (MQ=255)
                                                                        cATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                      <  1:1450128/148‑1 (MQ=255)
                                                                        cATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGGTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACggg                                                                      >  2:3297363/1‑148 (MQ=255)
                                                                         aTTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGa                                                                     <  2:1286818/148‑1 (MQ=255)
                                                                           tCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGaaa                                                                   <  2:1517607/148‑1 (MQ=255)
                                                                               gCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGACACCtt                                                               >  2:86240/1‑148 (MQ=255)
                                                                               gCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCAATCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCtt                                                               >  1:3012872/1‑148 (MQ=255)
                                                                                 aCTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCtttt                                                             <  1:1644752/148‑1 (MQ=255)
                                                                                 aCTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCtttt                                                             <  1:2237970/148‑1 (MQ=255)
                                                                                      tgctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCt                                                        <  2:1754595/148‑1 (MQ=255)
                                                                                      tgctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCACTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCt                                                        <  2:749099/148‑1 (MQ=255)
                                                                                       gctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTg                                                       >  1:1195055/1‑148 (MQ=255)
                                                                                        ctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCggg                                                      >  2:1012135/1‑148 (MQ=255)
                                                                                        ctCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTgg                                                      >  2:197967/1‑148 (MQ=255)
                                                                                          cgcgGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGAt                                                    >  2:3545020/1‑148 (MQ=255)
                                                                                            cgGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTa                                                  <  1:1770364/148‑1 (MQ=255)
                                                                                            cgGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTa                                                  <  2:313268/148‑1 (MQ=255)
                                                                                            cgGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTa                                                  <  2:3733363/148‑1 (MQ=255)
                                                                                               tCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTAGAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGa                                               <  2:3006764/148‑1 (MQ=255)
                                                                                                cGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGaa                                              >  1:2741934/1‑148 (MQ=255)
                                                                                                  ctctctGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACt                                            <  2:731712/148‑1 (MQ=255)
                                                                                                            atccatTCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAg                                  <  2:1235445/148‑1 (MQ=255)
                                                                                                                  tCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGcc                            >  2:2409529/1‑148 (MQ=255)
                                                                                                                  tCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGcc                            >  1:2945967/1‑148 (MQ=255)
                                                                                                                  tCCCTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGcc                            >  2:3495886/1‑148 (MQ=255)
                                                                                                                   cccTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCg                           >  1:3695535/1‑148 (MQ=255)
                                                                                                                    ccTGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGa                          <  2:1914361/148‑1 (MQ=255)
                                                                                                                      tGGTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGAtt                        >  1:413136/1‑148 (MQ=255)
                                                                                                                       ggTTTGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTa                       >  2:2851495/1‑148 (MQ=255)
                                                                                                                         tttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTAcc                     <  1:1079863/148‑1 (MQ=255)
                                                                                                                          ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCt                    <  1:3443650/148‑1 (MQ=255)
                                                                                                                          ttGGTAAAGCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCt                    <  1:389587/148‑1 (MQ=255)
                                                                                                                                  gCCGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaa            >  1:1297085/1‑148 (MQ=255)
                                                                                                                                   ccGACAACCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTaaa           <  2:3704348/148‑1 (MQ=255)
                                                                                                                                        aaCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGa      >  1:2120405/1‑148 (MQ=255)
                                                                                                                                        aaCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGa      <  1:3355696/148‑1 (MQ=255)
                                                                                                                                         aCCGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaa     <  2:446622/148‑1 (MQ=255)
                                                                                                                                          ccGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGaaa    >  1:3693537/1‑148 (MQ=255)
                                                                                                                                           cGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAg   >  1:1391736/1‑148 (MQ=255)
                                                                                                                                           cGTATAGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAg   <  1:2100047/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TCAAGCTGGGACGCGCACGACACAGAGAATTAACTAATTGAAAAAATTAAAGATTAATTATCTGTTCATCGGCATTCTGGCACTGCTGCTCGCGGTCGCTCTCTGGCCATCCATTCCCTGGTTTGGTAAAGCCGACAACCGTATCGCCGCCATTCAAGCGCGGGGAGAGTTGCGTGTGAGCACCATTCATACTCCCCTGACTTATAACGAAATCAACGGGAAACCTTTTGGCCTGGATTACGAACTGGCGAAACAGTTTGCCGATTACCTCGGCGTAAAACTGAAAGT  >  CP009273/2689123‑2689410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑