BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,778,820	G→A	100%	intergenic (‑450/+301)	ypjC ← / ← ileY	pseudogene/tRNA‑Ile

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,778,820	0	G	A	98.5%	912.3 / 2.1	273	intergenic (‑450/+301)	ypjC/ileY	pseudogene/tRNA‑Ile
Reads supporting (aligned to +/- strand): ref base G (3/1); new base A (109/160); total (112/161)
Fisher's exact test for biased strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

TGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAT  >  CP009273/2778677‑2778964

tgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                              >  2:1290648/1‑148 (MQ=255)

tgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGaataat                                                                                                                                              >  2:2521590/1‑148 (MQ=255)

    gcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAAc                                                                                                                                          <  2:402635/148‑1 (MQ=255)

     cggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAAcc                                                                                                                                         <  1:1811785/148‑1 (MQ=255)

           ttCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTa                                                                                                                                   >  1:947551/1‑148 (MQ=255)

            tCTGGTCAGTAGTAGATGTTTAAGGCGTGTCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTaa                                                                                                                                  <  1:3628787/148‑1 (MQ=255)

              tGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAAt                                                                                                                                <  1:1290495/148‑1 (MQ=255)

              tGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAAt                                                                                                                                <  1:1290756/148‑1 (MQ=255)

                gTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGa                                                                                                                              >  2:1761159/1‑148 (MQ=255)

                gTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGa                                                                                                                              <  1:3024923/148‑1 (MQ=255)

                 tCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGaa                                                                                                                             <  1:3235231/148‑1 (MQ=255)

                 tCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGaa                                                                                                                             <  2:2139939/148‑1 (MQ=255)

                 tCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGaa                                                                                                                             >  2:3489869/1‑148 (MQ=255)

                     tagtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAgggg                                                                                                                         >  2:1858061/1‑148 (MQ=255)

                      agtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGt                                                                                                                        <  1:1130361/148‑1 (MQ=255)

                       gtagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGta                                                                                                                       <  2:1212520/148‑1 (MQ=255)

                        tagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtat                                                                                                                      >  1:1326229/1‑148 (MQ=255)

                        tagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtat                                                                                                                      >  1:1221210/1‑148 (MQ=255)

                        tagATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtat                                                                                                                      >  1:1517198/1‑148 (MQ=255)

                         agATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCCCGTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtcta                                                                                                                     >  2:2815896/1‑148 (MQ=255)

                         agATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtata                                                                                                                     >  2:1774796/1‑148 (MQ=255)

                         agATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGtata                                                                                                                     <  1:2870015/148‑1 (MQ=255)

                          gATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTataa                                                                                                                    >  1:530993/1‑148 (MQ=255)

                           aTGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataat                                                                                                                   >  2:2999470/1‑148 (MQ=255)

                            tGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTataata                                                                                                                  >  2:636465/1‑148 (MQ=255)

                              tttAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATaaa                                                                                                                <  2:3035487/148‑1 (MQ=255)

                              tttAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATaaa                                                                                                                <  2:2291393/148‑1 (MQ=255)

                               ttAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAAt                                                                                                               >  2:565119/1‑148 (MQ=255)

                                tAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATg                                                                                                              <  2:2975169/148‑1 (MQ=255)

                                 aaGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGa                                                                                                             <  2:160625/148‑1 (MQ=255)

                                  aGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGat                                                                                                            >  1:1612063/1‑148 (MQ=255)

                                  aGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGat                                                                                                            >  1:1342422/1‑148 (MQ=255)

                                   ggCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGata                                                                                                           <  2:3107083/148‑1 (MQ=255)

                                   ggCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGata                                                                                                           >  1:3134962/1‑148 (MQ=255)

                                   ggCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGata                                                                                                           >  2:149566/1‑148 (MQ=255)

                                    gCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGataa                                                                                                          <  1:411612/148‑1 (MQ=255)

                                     cGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGataat                                                                                                         <  1:2469011/148‑1 (MQ=255)

                                             agaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGc                                                                                                 <  1:3558283/148‑1 (MQ=255)

                                              gaCATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCt                                                                                                <  1:2787899/148‑1 (MQ=255)

                                                cATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCttt                                                                                              <  1:821171/148‑1 (MQ=255)

                                                cATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCttt                                                                                              <  2:587475/148‑1 (MQ=255)

                                                 aTTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTa                                                                                             <  1:3370152/148‑1 (MQ=255)

                                                  tttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTAt                                                                                            <  1:616854/148‑1 (MQ=255)

                                                  tttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTAt                                                                                            <  1:2302711/148‑1 (MQ=255)

                                                   ttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATc                                                                                           >  1:1726317/1‑148 (MQ=255)

                                                   ttCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATc                                                                                           >  1:2103674/1‑148 (MQ=255)

                                                       tCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGTATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTa                                                                                       <  1:2408330/148‑1 (MQ=255)

                                                        ccTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTaa                                                                                      <  1:464966/148‑1 (MQ=255)

                                                        ccTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTaa                                                                                      <  1:290352/148‑1 (MQ=255)

                                                           tACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTCGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTCTCGTTAATTa                                                                                   >  2:2753795/1‑148 (MQ=255)

                                                             ctctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACt                                                                                 >  1:555632/1‑148 (MQ=255)

                                                              tctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACtt                                                                                <  2:3319802/148‑1 (MQ=255)

                                                               ctACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTa                                                                               <  1:874886/148‑1 (MQ=255)

                                                                tACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTaa                                                                              <  2:1057630/148‑1 (MQ=255)

                                                                   ggCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTc                                                                           <  1:262969/148‑1 (MQ=255)

                                                                   ggCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTc                                                                           <  2:1164236/148‑1 (MQ=255)

                                                                    gCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTcc                                                                          <  2:145872/148‑1 (MQ=255)

                                                                       ttGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCcaca                                                                       >  2:89355/1‑148 (MQ=255)

                                                                       ttGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCcaca                                                                       >  1:3493052/1‑148 (MQ=255)

                                                                        tGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACAt                                                                      >  1:3773145/1‑148 (MQ=255)

                                                                        tGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACAt                                                                      >  2:1652498/1‑148 (MQ=255)

                                                                        tGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACAt                                                                      >  1:3323597/1‑148 (MQ=255)

                                                                                 tacatTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAAt                                                             <  1:2333807/148‑1 (MQ=255)

                                                                                     ttGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGc                                                         <  1:2558006/148‑1 (MQ=255)

                                                                                      tGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGcc                                                        <  1:1115245/148‑1 (MQ=255)

                                                                                         ttGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGc                                                     >  2:1131229/1‑148 (MQ=255)

                                                                                            tgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTg                                                  <  2:3566682/148‑1 (MQ=255)

                                                                                            tgGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTg                                                  <  2:3568570/148‑1 (MQ=255)

                                                                                               tACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCa                                               <  1:1290683/148‑1 (MQ=255)

                                                                                                aCTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCAt                                              <  1:1652498/148‑1 (MQ=255)

                                                                                                  tCACTTATCATCAGTGAGCGAAGAGAGAATAGTTCAGTGATTTTAATTATTAACCTGATTAAATGAAGGTGTATAATAAATTATAATACTCTTTATTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGACCGCTTGGAATAg                                            <  2:551964/148‑1 (MQ=255)

                                                                                                     cTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAg                                         >  1:1909070/1‑148 (MQ=255)

                                                                                                      ttATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAgg                                        >  2:3676838/1‑148 (MQ=255)

                                                                                                       tATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGc                                       >  1:1746274/1‑148 (MQ=255)

                                                                                                        atcatcAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCa                                      >  2:2475854/1‑148 (MQ=255)

                                                                                                         tcatcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAt                                     >  2:2629630/1‑148 (MQ=255)

                                                                                                         tcatcaGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAt                                     >  1:2436586/1‑148 (MQ=255)

                                                                                                             caGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAttttt                                 >  2:1977795/1‑148 (MQ=255)

                                                                                                             caGTGAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCAttttt                                 >  1:1387362/1‑148 (MQ=255)

                                                                                                                 gAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCa                             <  2:842423/148‑1 (MQ=255)

                                                                                                                 gAGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCa                             <  1:278324/148‑1 (MQ=255)

                                                                                                                  aGCGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAg                            >  2:1015471/1‑148 (MQ=255)

                                                                                                                    cGAACAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGt                          >  1:904898/1‑148 (MQ=255)

                                                                                                                       aCAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACt                       >  2:2458149/1‑148 (MQ=255)

                                                                                                                       aCAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACt                       >  2:3067389/1‑148 (MQ=255)

                                                                                                                        cAGAGAATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACtt                      >  1:3113575/1‑148 (MQ=255)

                                                                                                                           agaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTg                   >  2:2645889/1‑148 (MQ=255)

                                                                                                                            gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGa                  <  2:3393855/148‑1 (MQ=255)

                                                                                                                            gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGa                  <  2:1395117/148‑1 (MQ=255)

                                                                                                                            gaATAGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGa                  <  2:946525/148‑1 (MQ=255)

                                                                                                                                aGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGa              <  1:1357865/148‑1 (MQ=255)

                                                                                                                                aGTTCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGa              >  2:106498/1‑148 (MQ=255)

                                                                                                                                  ttCAGTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGt            <  2:1726836/148‑1 (MQ=255)

                                                                                                                                      gTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTg        >  1:2300046/1‑148 (MQ=255)

                                                                                                                                      gTGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTg        <  1:636515/148‑1 (MQ=255)

                                                                                                                                       tGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGa       <  2:3261480/148‑1 (MQ=255)

                                                                                                                                       tGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGa       <  1:1946600/148‑1 (MQ=255)

                                                                                                                                       tGATTTGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGa       >  2:449173/1‑148 (MQ=255)

                                                                                                                                          tttGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTAc          >  1:1493430/1‑142 (MQ=255)

                                                                                                                                           ttGAATAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGa   >  1:1849379/1‑148 (MQ=255)

TGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTACTCACTTATCATCAGTGAGCGAACAGAGAATAGTTCAGTGATTTGAGTAATTAACCTGATTAAATGAAGGGGTATAATAAATGATAATACTCTGGCTTTATCGTTAATTACTTAATTCCACATGTAAGCAATTTGCCCGCTTGGCATAGCAGGCATTTTTTCCAGGTACTTTTGAATGAGTACTGATGGAT  >  CP009273/2778677‑2778964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`