BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,801,706	C→A	100%	pseudogene (32/1178 nt)	ygaY →	pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,801,706	0	C	A	98.6%	720.9 / 0.5	220	pseudogene (32/1178 nt)	ygaY	pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein
Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (131/86); total (132/88)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

ATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGC  >  CP009273/2801563‑2801848

aTCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTg                                                                                                                                            <  1:3474892/148‑1 (MQ=255)

 tCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGa                                                                                                                                           <  1:1015135/148‑1 (MQ=255)

 tCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGa                                                                                                                                           >  1:645259/1‑148 (MQ=255)

  cATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGAt                                                                                                                                          >  2:168221/1‑148 (MQ=255)

  cATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGAt                                                                                                                                          >  1:1564885/1‑148 (MQ=255)

   aTGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATc                                                                                                                                         >  1:81529/1‑148 (MQ=255)

       cTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGc                                                                                                                                     <  1:928679/148‑1 (MQ=255)

        tACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCt                                                                                                                                    >  1:1623047/1‑148 (MQ=255)

         aCAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTTTTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTg                                                                                                                                   <  2:2207959/148‑1 (MQ=255)

               cacaTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCt                                                                                                                             >  1:1482523/1‑148 (MQ=255)

                acctCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTa                                                                                                                            <  2:1399222/145‑1 (MQ=255)

                acaTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTa                                                                                                                            <  1:2129818/148‑1 (MQ=255)

                 caTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAt                                                                                                                           >  1:2887281/1‑148 (MQ=255)

                    cGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGc                                                                                                                        >  1:1956900/1‑148 (MQ=255)

                        caTTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCAcc                                                                                                                    >  1:2110089/1‑148 (MQ=255)

                           ttCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGt                                                                                                                 >  1:3766610/1‑148 (MQ=255)

                                  atctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCgg                                                                                                          >  1:115309/1‑148 (MQ=255)

                                   tctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGt                                                                                                         >  1:1356482/1‑148 (MQ=255)

                                    ctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCggga                                                                                                        >  2:2875124/1‑146 (MQ=255)

                                        ctTTCATCCCATATTCATCAACATCCGCTCTTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCGTATCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCa                                                                                                    >  2:2035675/1‑148 (MQ=255)

                                         tttCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAg                                                                                                   >  2:2225002/1‑148 (MQ=255)

                                            cATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTACACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTaa                                                                                                <  2:1443121/148‑1 (MQ=255)

                                               cccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTa                                                                                             >  1:783118/1‑148 (MQ=255)

                                               cccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTa                                                                                             >  2:1795676/1‑148 (MQ=255)

                                                ccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTAt                                                                                            <  2:1976858/148‑1 (MQ=255)

                                                ccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTAt                                                                                            >  1:2007741/1‑148 (MQ=255)

                                                  atatTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTa                                                                                          <  1:2847243/148‑1 (MQ=255)

                                                     ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGc                                                                                       >  2:1225269/1‑148 (MQ=255)

                                                       catcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGccc                                                                                     <  1:704623/148‑1 (MQ=255)

                                                       catcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGccc                                                                                     <  1:3420061/148‑1 (MQ=255)

                                                        atcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCa                                                                                    >  2:1045441/1‑148 (MQ=255)

                                                            aCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCa                                                                                >  2:2245755/1‑148 (MQ=255)

                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               <  2:70887/148‑1 (MQ=255)

                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               >  1:2197632/1‑148 (MQ=255)

                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               <  2:70246/148‑1 (MQ=255)

                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               <  1:3771093/148‑1 (MQ=255)

                                                               tCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTg                                                                             >  1:3585170/1‑148 (MQ=255)

                                                                ccGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGc                                                                            <  1:2127686/148‑1 (MQ=255)

                                                                 cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCt                                                                           >  2:2802607/1‑148 (MQ=255)

                                                                 cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCt                                                                           <  1:2515470/148‑1 (MQ=255)

                                                                   cTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCg                                                                         >  1:2931830/1‑148 (MQ=255)

                                                                   cTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCg                                                                         >  2:487332/1‑148 (MQ=255)

                                                                    tattatTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGa                                                                        <  2:863260/148‑1 (MQ=255)

                                                                    tagtattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGa                                                                        <  2:2776393/145‑1 (MQ=255)

                                                                       tattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGATGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGacac                                                                     >  1:2987741/1‑148 (MQ=255)

                                                                         ttGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCa                                                                   <  1:2578510/148‑1 (MQ=255)

                                                                                 cAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGt                                                           <  2:754748/148‑1 (MQ=255)

                                                                                 cAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGt                                                           >  2:1296647/1‑148 (MQ=255)

                                                                                  aGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTCTTACGCCCAGCCATTGCTCGACACCATCGCGCGTa                                                          >  2:1706526/1‑148 (MQ=255)

                                                                                   gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                         >  2:1969236/1‑148 (MQ=255)

                                                                                   gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                         <  2:308349/148‑1 (MQ=255)

                                                                                   gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                         <  2:3353356/148‑1 (MQ=255)

                                                                                     ttAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGAAACTATAACGCCCAGCCATTGATCGACACCATCGCGCGAAACt                                                       >  1:1490920/1‑148 (MQ=255)

                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTAGACACCATCGCGCGTAACtt                                                      >  2:358132/1‑148 (MQ=255)

                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      <  1:2172446/148‑1 (MQ=255)

                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      >  2:366708/1‑148 (MQ=255)

                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      <  1:244449/148‑1 (MQ=255)

                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      <  2:2264107/148‑1 (MQ=255)

                                                                                         tcatcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTc                                                   <  2:2784987/148‑1 (MQ=255)

                                                                                          catcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTcc                                                  >  1:1758386/1‑148 (MQ=255)

                                                                                           atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTccc                                                 >  1:1838682/1‑148 (MQ=255)

                                                                                           atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTccc                                                 <  1:122266/148‑1 (MQ=255)

                                                                                           atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTccc                                                 <  2:1844543/148‑1 (MQ=255)

                                                                                            tcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCt                                                <  2:2043499/148‑1 (MQ=255)

                                                                                             caCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCtt                                               >  1:492369/1‑148 (MQ=255)

                                                                                              aCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCttt                                              <  1:1054252/148‑1 (MQ=255)

                                                                                               ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTc                                             <  2:2914311/148‑1 (MQ=255)

                                                                                                 tgatgaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCg                                           >  1:1655844/1‑148 (MQ=255)

                                                                                                   atgaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTAGACACCATCGCGCGTAACTTTTCCCTTTACGcc                                         <  1:2563135/148‑1 (MQ=255)

                                                                                                      aaCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGt                                      <  2:642996/148‑1 (MQ=255)

                                                                                                      aaCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGt                                      <  2:2887158/148‑1 (MQ=255)

                                                                                                        cAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGACAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTc                                    >  2:2512705/1‑148 (MQ=255)

                                                                                                           aaaTAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGc                                 <  2:948889/148‑1 (MQ=255)

                                                                                                           aaaTAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGc                                 >  1:741770/1‑148 (MQ=255)

                                                                                                            aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                >  2:844015/1‑148 (MQ=255)

                                                                                                            aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                >  1:2912598/1‑148 (MQ=255)

                                                                                                            aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                >  1:876504/1‑148 (MQ=255)

                                                                                                              taatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAgg                              >  2:1358210/1‑148 (MQ=255)

                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            >  2:2057071/1‑148 (MQ=255)

                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            >  2:1151634/1‑148 (MQ=255)

                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            >  2:1077012/1‑148 (MQ=255)

                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            <  1:3220897/148‑1 (MQ=255)

                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         <  2:3035414/148‑1 (MQ=255)

                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         >  1:2419949/1‑148 (MQ=255)

                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         <  1:3264011/148‑1 (MQ=255)

                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         <  1:3263997/148‑1 (MQ=255)

                                                                                                                    cTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAt                        >  2:2047644/1‑148 (MQ=255)

                                                                                                                    cTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAt                        >  1:2135241/1‑148 (MQ=255)

                                                                                                                      aaaCCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTg                      <  1:635527/148‑1 (MQ=255)

                                                                                                                       aaCCTACTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGt                     <  1:3387989/148‑1 (MQ=255)

                                                                                                                          cTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAc                  <  2:401011/148‑1 (MQ=255)

                                                                                                                          cTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAc                  <  2:853668/148‑1 (MQ=255)

                                                                                                                            aaTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAccg                >  2:2318116/1‑148 (MQ=255)

                                                                                                                              tCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAccgcc              >  1:332212/1‑148 (MQ=255)

                                                                                                                                 tGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCcgcg           >  2:362221/1‑148 (MQ=255)

                                                                                                                                 tGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCcgcg           >  1:3345510/1‑148 (MQ=255)

                                                                                                                                     cTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGt       >  2:1549470/1‑148 (MQ=255)

                                                                                                                                       tAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTg     >  2:1193648/1‑148 (MQ=255)

                                                                                                                                         gCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTggg   <  2:2529225/148‑1 (MQ=255)

                                                                                                                                          cccGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGc  <  2:3610371/148‑1 (MQ=255)

ATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGC  >  CP009273/2801563‑2801848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`