Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,828,952 C→A 100% T611T (ACG→ACT)  hypF ← carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,828,9520CA99.5% 571.1 / ‑5.9 187T611T (ACG→ACT) hypFcarbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base A (92/92);  minor base T (0/2);  total (92/95)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

GTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA  >  CP009273/2828807‑2829096
                                                                                                                                                 |                                                                                                                                                
gTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGt                                                                                                                                                <  1:1762932/148‑1 (MQ=255)
 tgcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtg                                                                                                                                               <  1:3138921/148‑1 (MQ=255)
  gcctgccAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtgg                                                                                                                                              <  1:866738/148‑1 (MQ=255)
      gccAGTTCAGCCACTGCTGCCAGAAAGTGGCTAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGtggctgg                                                                                                                                          >  1:3342060/1‑148 (MQ=255)
           ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     >  1:3531627/1‑148 (MQ=255)
           ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     >  1:629170/1‑148 (MQ=255)
           ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     >  1:1637147/1‑148 (MQ=255)
           ttCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGcac                                                                                                                                     >  1:1267501/1‑148 (MQ=255)
               gCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGcc                                                                                                                                 <  2:693505/148‑1 (MQ=255)
                  aCTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAg                                                                                                                              <  2:1425162/148‑1 (MQ=255)
                   ctgctgCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGt                                                                                                                             >  1:1009649/1‑148 (MQ=255)
                    tgctgcCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTg                                                                                                                            >  2:3394033/1‑148 (MQ=255)
                            gAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTgccgccgcc                                                                                                                    >  2:3466329/1‑148 (MQ=255)
                             aaaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCa                                                                                                                   >  2:3651627/1‑148 (MQ=255)
                             aaaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCa                                                                                                                   >  1:2205164/1‑148 (MQ=255)
                             aaaGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCa                                                                                                                   >  1:2935737/1‑148 (MQ=255)
                               aGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACt                                                                                                                 >  2:1907145/1‑148 (MQ=255)
                                gTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTg                                                                                                                >  2:1110737/1‑148 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               >  2:1492717/1‑148 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               >  1:3681754/1‑148 (MQ=255)
                                 tGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGc                                                                                                               <  1:2812839/148‑1 (MQ=255)
                                        aTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaa                                                                                                        >  2:1181445/1‑148 (MQ=255)
                                        aTCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACGCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCGGCGTCACCTTCTGAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaa                                                                                                        >  1:1252866/1‑148 (MQ=255)
                                         tCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaa                                                                                                       >  1:372395/1‑148 (MQ=255)
                                          ccAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGaaaaa                                                                                                      <  2:496528/148‑1 (MQ=255)
                                             gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                   >  1:1802412/1‑148 (MQ=255)
                                             gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                   >  1:2443262/1‑148 (MQ=255)
                                             gTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACg                                                                                                   <  2:2010021/148‑1 (MQ=255)
                                              ttGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGc                                                                                                  >  1:2171475/1‑148 (MQ=255)
                                                gATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGccc                                                                                                <  2:787808/148‑1 (MQ=255)
                                                  ttGTCCCCCCGCGGCATTGTCCCCGGATGCGTCACGCCGTGGCTTGTGGCTGCGAGCGCCTCCAGCGCACAAGCGGCTACACCTTCATAACTTAATGTGGCGGGCGCACAGCCCTGGGCCGCCGCCACTGCATCGAAAAAACGCCcac                                                                                              <  1:1943332/148‑1 (MQ=255)
                                                  ttGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCcac                                                                                              >  1:1465454/1‑148 (MQ=255)
                                                   tGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCcaca                                                                                             >  2:1644195/1‑148 (MQ=255)
                                                     tCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACg                                                                                           >  2:623112/1‑148 (MQ=255)
                                                        aCCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATg                                                                                        <  2:2054920/148‑1 (MQ=255)
                                                           cgcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACg                                                                                     >  1:1185094/1‑148 (MQ=255)
                                                            gcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAGAAACGCCCACACGATGACGc                                                                                    <  1:1417758/148‑1 (MQ=255)
                                                            gcgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGc                                                                                    <  1:3471200/148‑1 (MQ=255)
                                                             cgGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGcc                                                                                   >  2:920493/1‑148 (MQ=255)
                                                                  ttGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcgg                                                                              >  2:3053951/1‑148 (MQ=255)
                                                                    gTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcggcg                                                                            <  2:2726311/148‑1 (MQ=255)
                                                                    gTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAgcggcg                                                                            <  2:2035859/148‑1 (MQ=255)
                                                                      cACCGGATGCGTCACTCCGTGGCTTGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGGGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGcgcg                                                                          <  1:1102556/148‑1 (MQ=255)
                                                                      cACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGcgcg                                                                          <  2:2321562/148‑1 (MQ=255)
                                                                        ccGGATGCGTCACTCCGTGGCATGAGGCTGCGATCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGtt                                                                        <  2:2669351/148‑1 (MQ=255)
                                                                        ccGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGtt                                                                        <  2:2534891/148‑1 (MQ=255)
                                                                         cGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTa                                                                       >  1:889480/1‑148 (MQ=255)
                                                                          ggATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTaa                                                                      <  2:561723/148‑1 (MQ=255)
                                                                                 tCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACg                                                               >  2:1188934/1‑148 (MQ=255)
                                                                                  cACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGc                                                              >  2:2346611/1‑148 (MQ=255)
                                                                                  cACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGc                                                              >  2:2070593/1‑148 (MQ=255)
                                                                                   aCTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCt                                                             <  2:49641/148‑1 (MQ=255)
                                                                                    cTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTc                                                            >  1:2586811/1‑148 (MQ=255)
                                                                                     tCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCa                                                           <  1:2483854/148‑1 (MQ=255)
                                                                                      ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          <  2:1026265/148‑1 (MQ=255)
                                                                                      ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          <  1:530057/148‑1 (MQ=255)
                                                                                      ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCaa                                                          <  2:1988808/148‑1 (MQ=255)
                                                                                      ccGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCGTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTCATTCCACGCTCaa                                                          >  1:2340663/1‑148 (MQ=255)
                                                                                         tGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGGTAATTCCACGCTCAATgg                                                       >  2:1923462/1‑148 (MQ=255)
                                                                                              tGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgc                                                  >  1:2401648/1‑148 (MQ=255)
                                                                                              tGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCcgc                                                  >  1:2401661/1‑148 (MQ=255)
                                                                                                    tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGc                                            <  2:3715318/148‑1 (MQ=255)
                                                                                                    tGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGc                                            <  2:2594281/148‑1 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      >  1:1225204/1‑148 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      >  1:1926245/1‑148 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      <  2:304108/148‑1 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      >  2:112519/1‑148 (MQ=255)
                                                                                                          cgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTc                                      <  2:2161641/148‑1 (MQ=255)
                                                                                                            ccTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCa                                    >  1:471344/1‑148 (MQ=255)
                                                                                                            ccTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGAGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCa                                    >  1:1008367/1‑148 (MQ=255)
                                                                                                             cTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAg                                   <  2:1256010/148‑1 (MQ=255)
                                                                                                             cTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGGGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAg                                   <  2:332404/148‑1 (MQ=255)
                                                                                                             cTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCACTGCCGCCGCCACTGCATCGAAAAAACCCCCACACGAAGACGCCAGCGGCGCGTTAATTCCACCCTCAATGGCCCGCGCCAGCACGCTCCAg                                   >  2:2989073/1‑148 (MQ=255)
                                                                                                              cccAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGt                                  <  1:2350928/147‑1 (MQ=255)
                                                                                                                cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  2:2139637/148‑1 (MQ=255)
                                                                                                                cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  2:1267546/148‑1 (MQ=255)
                                                                                                                cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  2:3531627/148‑1 (MQ=255)
                                                                                                                cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  2:1637147/148‑1 (MQ=255)
                                                                                                                cAGAGCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGttt                                <  2:1267501/148‑1 (MQ=255)
                                                                                                                  gagCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTg                              <  1:1540747/148‑1 (MQ=255)
                                                                                                                   agCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                             >  2:2427807/1‑148 (MQ=255)
                                                                                                                   agCACAAGCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGc                             >  1:1089512/1‑148 (MQ=255)
                                                                                                                          gCCGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAAAGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGca                      >  2:1150424/1‑148 (MQ=255)
                                                                                                                            cGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcaca                    <  1:3750659/148‑1 (MQ=255)
                                                                                                                            cGCTTCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGcaca                    <  1:2209955/148‑1 (MQ=255)
                                                                                                                                tCACCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTg                <  1:3417778/148‑1 (MQ=255)
                                                                                                                                  aCCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCt              >  2:807187/1‑148 (MQ=255)
                                                                                                                                  aCCTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGGTGCACACTTGCt              >  2:249230/1‑148 (MQ=255)
                                                                                                                                   ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTg             >  2:2716945/1‑148 (MQ=255)
                                                                                                                                   ccTTCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTAAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTg             >  2:2717452/1‑148 (MQ=255)
                                                                                                                                     ttCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGtt           >  2:3545676/1‑148 (MQ=255)
                                                                                                                                     ttCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGGTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGtt           >  1:2123527/1‑148 (MQ=255)
                                                                                                                                      tCATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGttt          <  1:1024565/148‑1 (MQ=255)
                                                                                                                                       cATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTc         <  2:3234/148‑1 (MQ=255)
                                                                                                                                       cATAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTc         <  2:1616594/148‑1 (MQ=255)
                                                                                                                                         tAACTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag       >  1:754941/1‑148 (MQ=255)
                                                                                                                                          aaCTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCaga      >  1:390112/1‑148 (MQ=255)
                                                                                                                                            cTTAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt    <  2:1518655/148‑1 (MQ=255)
                                                                                                                                             ttAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTa   <  2:2652770/148‑1 (MQ=255)
                                                                                                                                              tAAAGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTaa  <  1:3787589/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGCCTGCCAGTTCAGCCACTGCTGCCAGAAAGTGGCGAGATCCAGTTGATTGTCCACCCGCGGCATTGTCACCGGATGCGTCACTCCGTGGCATGAGGCTGCGAGCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAA  >  CP009273/2828807‑2829096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑