Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,835,291 T→G 9.7% C315G (TGC→GGC)  ascB → cryptic 6‑phospho‑beta‑glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,835,2910TG 9.7% 493.4 / 6.5 220C315G (TGC→GGC) ascBcryptic 6‑phospho‑beta‑glucosidase
Reads supporting (aligned to +/- strand):  ref base T (99/93);  new base G (25/3);  total (124/96)
Fisher's exact test for biased strand distribution p-value = 1.47e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTT  >  CP009273/2835145‑2835438
                                                                                                                                                  |                                                                                                                                                   
   aTGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTGTCTTTCAGCTATTACGCCTCGCGCTgcgc                                                                                                                                                 >  2:1639069/1‑148 (MQ=255)
   aTGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgcgc                                                                                                                                                 <  1:2722239/148‑1 (MQ=255)
   aTGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgcgc                                                                                                                                                 >  2:2907162/1‑148 (MQ=255)
   aTGTGCAGGCGCGGGGCACGTATCCGGCTCACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTgcgc                                                                                                                                                 >  2:3609972/1‑148 (MQ=255)
     gtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCGTTGAGCGATTACCCCTCGCGCGGCGccg                                                                                                                                               >  1:183508/1‑147 (MQ=255)
     gtgCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCt                                                                                                                                               <  2:1247748/148‑1 (MQ=255)
          ggCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTAGTTCAGCTATTACGCCTCGCGCGGCGCCTcggcg                                                                                                                                          >  1:743689/1‑148 (MQ=255)
           gcgcGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCGcggcgg                                                                                                                                         >  2:3292546/1‑148 (MQ=255)
             gcgGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGgag                                                                                                                                       <  2:50099/148‑1 (MQ=255)
                 ggCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATGTTGTCTCTTTAAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGa                                                                                                                                   >  2:2674379/1‑148 (MQ=255)
                        aTCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCcaa                                                                                                                            <  2:1815769/148‑1 (MQ=255)
                          ccGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTAAGCTATTACGCCTCGCGCTGCGCCTCGGCGGCGATGAACCCcaaaa                                                                                                                          >  1:3710729/1‑146 (MQ=255)
                          ccGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCcaaca                                                                                                                          <  2:3281366/148‑1 (MQ=255)
                          ccGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTCTTTGAGGGATTAAGCCTCGCGCGGCGCCGCGGCGGGGAGGAACGCccaca                                                                                                                          >  1:1099145/1‑148 (MQ=255)
                             gCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAacaaca                                                                                                                       <  1:2060267/148‑1 (MQ=255)
                               ttACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAAcagc                                                                                                                     <  2:684382/148‑1 (MQ=255)
                                   tctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCGTTCAGCTATTAAGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTg                                                                                                                 >  2:1976040/1‑148 (MQ=255)
                                    ctGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgc                                                                                                                <  2:2542831/148‑1 (MQ=255)
                                      gCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTgcgg                                                                                                              <  2:559704/148‑1 (MQ=255)
                                        ccGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCNATTTTTTGTATTTAAGCGAATACGCCACCCGCGGCGCCACCGCGGCGATTAACCCCACCACAAACACTgcggcg                                                                                                            >  1:1934286/1‑148 (MQ=255)
                                          gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTAATCTATTAAGCCTCGCGCGGCGCCGCGGCGGGGATGAAAGCCAACAACACCAGTGCGGCGaa                                                                                                          >  1:2216567/1‑148 (MQ=255)
                                          gcgTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTCTCTTTCAGCTATTAAGCCGCGCGCTGCGCCTCGGCGGAGATGAAAGCCAACAAAAGCAGTGCGGCGaa                                                                                                          >  1:2240834/1‑148 (MQ=255)
                                               ttCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtgg                                                                                                     <  1:3609972/148‑1 (MQ=255)
                                               ttCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACgtgg                                                                                                     >  2:2405739/1‑148 (MQ=255)
                                                    cgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGaaa                                                                                                <  1:1497228/148‑1 (MQ=255)
                                                    cgAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGaaa                                                                                                <  2:3007386/148‑1 (MQ=255)
                                                     gAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAAt                                                                                               <  2:1945075/148‑1 (MQ=255)
                                                     gAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAAt                                                                                               <  2:1944908/148‑1 (MQ=255)
                                                     gAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAAt                                                                                               <  2:1001609/148‑1 (MQ=255)
                                                       aaaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCg                                                                                             <  2:1439333/148‑1 (MQ=255)
                                                        aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTTTGTCTTTGAGCTATTAAGCCGCGCCCGGCGCCGCCGCGGAGATTAACGCCAACAACAACAGTGCGGCGAACCGGGTGAAATAGc                                                                                            >  1:832831/1‑148 (MQ=255)
                                                        aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGc                                                                                            <  2:543315/148‑1 (MQ=255)
                                                        aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCGCGCGCTGCGCCGCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGGACGGGGTGAAATCGc                                                                                            >  2:1176148/1‑148 (MQ=255)
                                                        aaaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCGATTACGCCTCGCGCTGCGCCACGGCGGAGATGAACGCCAAAAACAGCAGTGCGGGGAACGGGGTGTAATCGc                                                                                            >  2:1334905/1‑148 (MQ=255)
                                                         aaGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTAAGCTATTACGCCTCGCGCTGCGCCTCGGCGGGGGTGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGcg                                                                                           >  2:2757791/1‑147 (MQ=255)
                                                           ggggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTGTTTAATCTATTAAGCCTCGCCCGGCGCCACCGCCGGGGTTAAAGCCCACAAAAACACTGCGGCGCACCGGGTGAAATagcagc                                                                                         >  1:3210730/1‑148 (MQ=255)
                                                           ggggTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTATCGTTAAGCTATTAAGCCACCCGCGGGGCCCCCGGGGCGATTAACGCCCACAAAAACAATGCCGCGCAACTGGTGgaaaagcggc                                                                                         >  1:2602577/1‑141 (MQ=255)
                                                                 aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATc                                                                                   <  1:3137493/148‑1 (MQ=255)
                                                                 aCCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTATCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTAATc                                                                                   >  2:2855781/1‑148 (MQ=255)
                                                                  ccgtCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTATCTTTCAGCGATTTACCCGCGCGCGGCGCCGCGGCGGAGATGAACGCCAACAACAGCCGTGGGGCGACCGGGGTGTAAAAGCTGCGGAAtac                                                                                  >  2:970448/4‑146 (MQ=255)
                                                                  ccATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTGTGGTTGAGCGATTACGCCGCCCGCGGCGCCGCGGCGGCGATGAACCCCCACAACACCCCGGGGGCGCACCGGGTGGAAACGCTGCGGAAtac                                                                                  >  1:2014179/1‑146 (MQ=255)
                                                                          aaaGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTAc                                                                          <  1:432323/148‑1 (MQ=255)
                                                                             gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTTTGTGTTTCAGCTATTACGCCTCGCGCGGCGCCGCGGCGGCGATGAACGCCAACAACACCAGTGCGGCGAACGTGGGGTAATCGGTGCGTAATACGTATCTAAAgg                                                                       >  1:2140132/1‑148 (MQ=255)
                                                                             gCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCGGCGTAATCCGTATCTACAgg                                                                       >  2:2225001/1‑148 (MQ=255)
                                                                                  gggCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCGTTGCGCTATTAAGCCTCGCGCTGCGCCTCGGCGGAGATTAAAGCCAACAACAGCAGTGCGGCGCACCTGGTGAAAACGATGCGTAATACGTATCTAAATGTGAGc                                                                  >  2:2495483/1‑148 (MQ=255)
                                                                                   ggCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCg                                                                 <  2:643088/148‑1 (MQ=255)
                                                                                   ggCGATGATGAAATCCTGAAAAACACCGTCGAGTTTGTATCTTTAAGCTATTAACCCACGCGCGGCGCCTCGGCGGCGATGAAACCCCACAACACCAGTGCGGCGCACGTGGTGAAATCGCTGCGTAATCCGGATCTACAGGTGAGCg                                                                 >  2:2591693/1‑148 (MQ=255)
                                                                                    gctatgatgATATCCTGAAAAACACCGTCGTTTTTGTCTCTGTCAGCTATTACGGCTCGCGCTGCGCCTCGGAGTAGATTAACGCCAACAACAGAAGTGCGGCGAACGTGGTGAAATCTATGCGTAATCCGTATCTAGAGGTGATCGa                                                                <  2:3480655/145‑1 (MQ=255)
                                                                                    gCGATGATGAAATCCTTAAAAAGACCGTCGATTTTTTCTCTTTCCGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTTATCCGTATCTACAGGTGAGCGa                                                                <  2:431392/148‑1 (MQ=255)
                                                                                    gCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGa                                                                <  2:2393704/148‑1 (MQ=255)
                                                                                    gCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGa                                                                <  1:3795394/148‑1 (MQ=255)
                                                                                       atgatgAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActg                                                             >  2:1283547/1‑148 (MQ=255)
                                                                                        tgatgaAATCCTGAAAAACACCGTCGATTTTGTATCTTTAAGCTATTAAGCCACCCGCTGCGCCTCGGCGGAGATTAACGCCAACAAAAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGAcggg                                                            >  2:109346/1‑148 (MQ=255)
                                                                                               aTCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGActggggctggg                                                     <  2:2151611/148‑1 (MQ=255)
                                                                                                     aaaaaCACCGTCGATTTTTTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAAACCCCACAACAGCAGTGCGGCGAACGGGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGGCGGGGGGGGGGGAAttt                                               >  2:2371340/1‑147 (MQ=255)
                                                                                                     aaaaaCACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGGGGTGAAAACGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCGGGGGCATTg                                               >  2:2373367/1‑148 (MQ=255)
                                                                                                      aaaaCACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGa                                              <  2:1136594/148‑1 (MQ=255)
                                                                                                      aaaaCACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGa                                              <  2:1099154/148‑1 (MQ=255)
                                                                                                       aaaCACCGTCGAGTTTTTCTCTTTCAGCTATTACGCCACGCGCTGCGCCTCGGCGGAGAAGAACGCCAACAACAGCACTGCGGCGAACGGGGGGAAATCGCTGCGGAATCCGTATCTACAGGTGAGCGCCTGGGGCGGGGGAAATGAt                                             >  2:3391422/1‑148 (MQ=255)
                                                                                                          cacCGTCGATTTTGTCTCTTTCAGCTATTACGCCGCGCGCTGCGCCTCGGCGGAGATGAACGCCAAAAACAGCAGTGCGGCGAACGGGGTGAAATAGCTGCGTAATCCGTATCTACACGTGAGCGACTGGGGCTGGGGAATTGATCCa                                          >  2:1552465/1‑148 (MQ=255)
                                                                                                              gTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCg                                      >  2:3138115/1‑148 (MQ=255)
                                                                                                                 gATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTc                                   <  2:3352560/148‑1 (MQ=255)
                                                                                                                  aTTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCt                                  >  2:202064/1‑148 (MQ=255)
                                                                                                                  aTTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCt                                  >  2:2987814/1‑148 (MQ=255)
                                                                                                                  aTTTTGTCTCTTACAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCAACTCGGTCt                                  >  2:3521048/1‑148 (MQ=255)
                                                                                                                    tttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGc                                >  1:1280287/1‑148 (MQ=255)
                                                                                                                    tttGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGc                                >  2:1189985/1‑148 (MQ=255)
                                                                                                                      tGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGt                              <  2:2117882/148‑1 (MQ=255)
                                                                                                                        tctcTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTAt                            >  1:574770/1‑148 (MQ=255)
                                                                                                                          tctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCa                          >  1:50082/1‑148 (MQ=255)
                                                                                                                          tctTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCa                          >  1:3002478/1‑148 (MQ=255)
                                                                                                                             ttCAGCTAATACGCCACGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCAGGGGAATTGATCCACTCGGTCTCCGTaaaaaca                       >  2:444389/1‑142 (MQ=255)
                                                                                                                               cAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATg                     >  1:1345602/1‑148 (MQ=255)
                                                                                                                                 gCTTTTACGCCGCGCGCGGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAATTCGCTGCGTAATCCGTATCTACTGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGaa                   <  1:2338159/148‑1 (MQ=255)
                                                                                                                                  cTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                  >  1:1020270/1‑148 (MQ=255)
                                                                                                                                  cTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAat                  >  2:1193466/1‑148 (MQ=255)
                                                                                                                                   tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCTACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                 >  1:1704176/1‑148 (MQ=255)
                                                                                                                                   tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                 >  1:3300602/1‑148 (MQ=255)
                                                                                                                                   tATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAata                 <  2:37177/148‑1 (MQ=255)
                                                                                                                                    aTTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAatat                >  2:1584113/1‑148 (MQ=255)
                                                                                                                                     ttACGCCTCTCGCTGCGCTTGGGCTGAGATGAACGCCAACAACCGCAGTGCGGCGAACGTGGTGAAATCGGTGCTTAATCCGTATTTACAGTTGCGCGACTGGGGCTGGGGAATTGATCCTCTAGGTCTGCGTATCACCATGAATatg               <  1:1127032/148‑1 (MQ=255)
                                                                                                                                     ttACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatg               <  1:477168/148‑1 (MQ=255)
                                                                                                                                      tccGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga              <  1:3413707/146‑1 (MQ=255)
                                                                                                                                      tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga              >  1:2468579/1‑148 (MQ=255)
                                                                                                                                      tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga              <  2:2284197/148‑1 (MQ=255)
                                                                                                                                      tACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatga              >  1:1729138/1‑148 (MQ=255)
                                                                                                                                        cGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg            >  2:1024776/1‑148 (MQ=255)
                                                                                                                                        cGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATatgatg            >  2:3744874/1‑148 (MQ=255)
                                                                                                                                         gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGt           <  2:900865/148‑1 (MQ=255)
                                                                                                                                         gCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGt           >  1:2983867/1‑148 (MQ=255)
                                                                                                                                          ccTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTa          <  1:3275428/148‑1 (MQ=255)
                                                                                                                                            tCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACg        >  2:1720163/1‑148 (MQ=255)
                                                                                                                                            tCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACg        >  2:2513675/1‑148 (MQ=255)
                                                                                                                                             cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa       >  2:3698945/1‑148 (MQ=255)
                                                                                                                                             cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa       <  2:1133780/148‑1 (MQ=255)
                                                                                                                                             cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa       >  1:334061/1‑148 (MQ=255)
                                                                                                                                             cgcgCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGa       <  1:3327492/148‑1 (MQ=255)
                                                                                                                                              gcgcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGAt      <  2:3210730/148‑1 (MQ=255)
                                                                                                                                                gcTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTTAAATCGCTGCGTAAGCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCg    <  1:1376648/148‑1 (MQ=255)
                                                                                                                                                  tGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGtt  <  1:1216653/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TCGATGTGCAGGCGCGGGGCACGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCAACAAAGCACCGGGCGATGATGAAATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATGAACGCCAACAACAGCAGTGCGGCGAACGTGGTGAAATCGCTGCGTAATCCGTATCTACAGGTGAGCGACTGGGGCTGGGGAATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGATCGTT  >  CP009273/2835145‑2835438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑