Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,836,554 G→T 100% I84I (ATC→ATA)  hycH ← hydrogenase 3 maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,836,5540GT99.0% 641.4 / ‑1.9 196I84I (ATC→ATA) hycHhydrogenase 3 maturation protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (101/93);  total (103/93)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

TCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGA  >  CP009273/2836409‑2836698
                                                                                                                                                 |                                                                                                                                                
 cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                               >  1:2727764/1‑148 (MQ=255)
 cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                               <  1:770714/148‑1 (MQ=255)
 cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                               <  2:160849/148‑1 (MQ=255)
 cctcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATg                                                                                                                                               <  2:2090105/148‑1 (MQ=255)
   tcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGAc                                                                                                                                             >  1:3043043/1‑148 (MQ=255)
   tcACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGAc                                                                                                                                             >  1:2615571/1‑148 (MQ=255)
    cACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACg                                                                                                                                            >  1:2798217/1‑148 (MQ=255)
     aCCATCAAATAGATGGCGTTTTACTGATGAATATCATGCAGCATACTTAACATCATTGTACTCCATTCCTCCTGCTGCGAAGTTTGCTCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTAAGACGa                                                                                                                                           >  2:3517497/1‑148 (MQ=255)
       cATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtc                                                                                                                                         >  1:2934240/1‑148 (MQ=255)
        aTCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtct                                                                                                                                        <  1:1702792/148‑1 (MQ=255)
         tCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGAtctc                                                                                                                                       >  1:883465/1‑148 (MQ=255)
           aaaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAc                                                                                                                                     <  2:1134657/148‑1 (MQ=255)
            aaTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCAcc                                                                                                                                    >  1:311538/1‑148 (MQ=255)
             aTAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCa                                                                                                                                   >  2:6796/1‑148 (MQ=255)
               aGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCata                                                                                                                                 <  1:1752852/148‑1 (MQ=255)
                 aTGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCAGTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATAtt                                                                                                                               <  2:110137/148‑1 (MQ=255)
                   ggCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTc                                                                                                                             >  2:1288643/1‑148 (MQ=255)
                     cGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCgg                                                                                                                           >  1:3172542/1‑148 (MQ=255)
                       ttttCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCa                                                                                                                         <  1:630179/148‑1 (MQ=255)
                       ttttCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCa                                                                                                                         <  1:2748167/148‑1 (MQ=255)
                               atgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTcc                                                                                                                 >  1:350525/1‑148 (MQ=255)
                                tgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCa                                                                                                                <  1:3762913/148‑1 (MQ=255)
                                tgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCa                                                                                                                <  1:3762814/148‑1 (MQ=255)
                                tgaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCa                                                                                                                >  1:2386656/1‑148 (MQ=255)
                                 gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAt                                                                                                               >  1:2512250/1‑148 (MQ=255)
                                 gaATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAt                                                                                                               >  1:1797344/1‑148 (MQ=255)
                                   atatCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCAttt                                                                                                             >  1:1209433/1‑148 (MQ=255)
                                     atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                           >  1:318632/1‑148 (MQ=255)
                                     atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                           >  1:275547/1‑148 (MQ=255)
                                     atCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTg                                                                                                           >  1:1463758/1‑148 (MQ=255)
                                          gcagcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTgcggcg                                                                                                      <  1:2081746/148‑1 (MQ=255)
                                            agcaTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGACTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCggg                                                                                                    <  2:2913828/148‑1 (MQ=255)
                                               aTACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCtt                                                                                                 <  2:1757515/148‑1 (MQ=255)
                                                tACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTc                                                                                                >  2:913830/1‑148 (MQ=255)
                                                 aCTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCa                                                                                               >  2:3134750/1‑148 (MQ=255)
                                                    tAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActg                                                                                            >  1:2173279/1‑148 (MQ=255)
                                                    tAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActg                                                                                            <  1:1024761/148‑1 (MQ=255)
                                                    tAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActg                                                                                            <  1:2429604/148‑1 (MQ=255)
                                                    tAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActg                                                                                            <  1:1024900/148‑1 (MQ=255)
                                                     aaCAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgc                                                                                           <  1:2477317/148‑1 (MQ=255)
                                                     aaCAGCATTGTACTCCATGCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgc                                                                                           <  2:1350421/148‑1 (MQ=255)
                                                      aCAGCATTGTACTCCAGTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcc                                                                                          <  2:870602/148‑1 (MQ=255)
                                                         gCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCActgcctgc                                                                                       <  1:763608/148‑1 (MQ=255)
                                                          cATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACtgcctgcc                                                                                      >  1:1153089/1‑148 (MQ=255)
                                                            ttGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    <  1:3279585/148‑1 (MQ=255)
                                                            ttGTACTCCAGTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTc                                                                                    <  1:172430/148‑1 (MQ=255)
                                                             tGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                                                                   >  2:720037/1‑148 (MQ=255)
                                                             tGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTcc                                                                                   >  2:2635429/1‑148 (MQ=255)
                                                               tACTCCAGTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCag                                                                                 <  1:453117/148‑1 (MQ=255)
                                                                aCTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCaga                                                                                >  2:705383/1‑148 (MQ=255)
                                                                aCTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCaga                                                                                >  1:1469514/1‑148 (MQ=255)
                                                                  tCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGt                                                                              >  2:1685811/1‑148 (MQ=255)
                                                                   ccATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGACTGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                             <  2:120015/148‑1 (MQ=255)
                                                                   ccATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTg                                                                             >  2:1383782/1‑148 (MQ=255)
                                                                    cATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTgg                                                                            <  1:295986/148‑1 (MQ=255)
                                                                     aTTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGc                                                                           >  1:1501107/1‑148 (MQ=255)
                                                                      ttgctgctgctgCGAAGTTTGCGCTGCTCGGTCTTTATAGAATGCGTTGGCCAGCATCTGCACATGGTTAATGTCTATGACGATCTCACCATATTTAGGCACGCCTTCCATTTTGCGGCGGGATTCACTGCCTTCCTCAAGAGTGGCa                                                                          <  2:1452281/145‑1 (MQ=255)
                                                                      ttCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCa                                                                          >  2:2613110/1‑148 (MQ=255)
                                                                      ttCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCa                                                                          >  2:3360646/1‑148 (MQ=255)
                                                                      ttCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCa                                                                          >  2:1619159/1‑148 (MQ=255)
                                                                          tgctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATcc                                                                      >  2:3571327/1‑148 (MQ=255)
                                                                           gctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCa                                                                     >  1:2846500/1‑148 (MQ=255)
                                                                           gctgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCa                                                                     <  1:2395384/148‑1 (MQ=255)
                                                                             tgctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATg                                                                   <  2:3486905/148‑1 (MQ=255)
                                                                              gctgcGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGc                                                                  <  1:507301/148‑1 (MQ=255)
                                                                                  cGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGa                                                              <  2:412365/148‑1 (MQ=255)
                                                                                   gAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGat                                                             <  1:6796/148‑1 (MQ=255)
                                                                                    aaGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGata                                                            >  2:433896/1‑148 (MQ=255)
                                                                                            gcTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATccc                                                    <  1:956749/148‑1 (MQ=255)
                                                                                             cTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCa                                                   >  2:1411748/1‑148 (MQ=255)
                                                                                              tGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCAc                                                  >  2:2733164/1‑148 (MQ=255)
                                                                                               gCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACg                                                 <  2:1209433/148‑1 (MQ=255)
                                                                                                  cGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGc                                              <  2:2692361/148‑1 (MQ=255)
                                                                                                      cTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGt                                          <  2:3478693/148‑1 (MQ=255)
                                                                                                      cTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGt                                          >  1:2339638/1‑148 (MQ=255)
                                                                                                       tttATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTg                                         <  2:2452151/148‑1 (MQ=255)
                                                                                                       tttATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTg                                         >  1:2457152/1‑148 (MQ=255)
                                                                                                          aTCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAgc                                      >  2:1980638/1‑148 (MQ=255)
                                                                                                            cGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAgcgc                                    >  2:1827732/1‑148 (MQ=255)
                                                                                                                tGCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCt                                >  1:2006506/1‑148 (MQ=255)
                                                                                                                 gCGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCtt                               >  2:2057909/1‑148 (MQ=255)
                                                                                                                  cGTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTc                              <  2:3725263/148‑1 (MQ=255)
                                                                                                                   gTTGGCCAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTcc                             <  2:2644750/148‑1 (MQ=255)
                                                                                                                         cAGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCaa                       <  2:1393015/148‑1 (MQ=255)
                                                                                                                          aGCATCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAAt                      <  2:627775/148‑1 (MQ=255)
                                                                                                                              tCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGAt                  >  1:1113346/1‑148 (MQ=255)
                                                                                                                              tCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGAt                  >  1:2690776/1‑148 (MQ=255)
                                                                                                                              tCGGCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGAt                  >  2:2439217/1‑148 (MQ=255)
                                                                                                                                ggCACATGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATaa                <  1:627806/148‑1 (MQ=255)
                                                                                                                                  cacaTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAcc              <  1:3477843/148‑1 (MQ=255)
                                                                                                                                   acaTGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAAccc             >  2:2223618/1‑148 (MQ=255)
                                                                                                                                      tGGTTAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCaa          >  2:3444158/1‑148 (MQ=255)
                                                                                                                                         ttAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggt       >  2:2656910/1‑148 (MQ=255)
                                                                                                                                          tAATGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtg      <  1:1329932/148‑1 (MQ=255)
                                                                                                                                           aaTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtgg     <  2:2988812/148‑1 (MQ=255)
                                                                                                                                           aaTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtgg     <  2:2798217/148‑1 (MQ=255)
                                                                                                                                            aTGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAggtggt    >  2:3035947/1‑148 (MQ=255)
                                                                                                                                             tGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGgtggtg   >  1:841112/1‑148 (MQ=255)
                                                                                                                                             tGTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGgtggtg   <  2:1501107/148‑1 (MQ=255)
                                                                                                                                              gTCTATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGa  >  1:2270305/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTACTCCATTCCTGCTGCTGCGAAGTTTGCGCTGCCCGGGCTTTATCGAATGCGTTGGCCAGCATCGGCACATGGTTAATGTCGATGACGATCTCACCATATTTCGGCACGCCTTCCATTTTGCGGCGGGCTTCACTGCCTGCCTCCAGAGTGGCAATCCATGCCAGATATTCATCCCACGGGCAGGTGAGCGCCGCTTCCAGGCAATCGATAACCCCAAGGTGGTGA  >  CP009273/2836409‑2836698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑