Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,846,112 C→A 100% I206I (ATC→ATA)  hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,846,1120CA99.2% 760.3 / ‑1.7 237I206I (ATC→ATA) hypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (120/115);  total (122/115)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

ACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAT  >  CP009273/2845971‑2846256
                                                                                                                                             |                                                                                                                                                
 cAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCg                                                                                                                                           <  1:3351053/148‑1 (MQ=255)
 cAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCg                                                                                                                                           <  2:2600062/148‑1 (MQ=255)
    gCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACg                                                                                                                                        <  1:895645/148‑1 (MQ=255)
    gCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACg                                                                                                                                        >  1:1166554/1‑148 (MQ=255)
     cGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGc                                                                                                                                       <  1:303833/148‑1 (MQ=255)
      gAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGcc                                                                                                                                      <  2:3618396/148‑1 (MQ=255)
         aGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCtat                                                                                                                                   >  1:3453981/1‑148 (MQ=255)
           cgcgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATaa                                                                                                                                 <  2:2255300/148‑1 (MQ=255)
           cgcgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATaa                                                                                                                                 <  2:379728/148‑1 (MQ=255)
            gcgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAt                                                                                                                                <  2:2926711/148‑1 (MQ=255)
            gcgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAt                                                                                                                                <  1:419683/148‑1 (MQ=255)
             cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtt                                                                                                                               >  1:1374953/1‑148 (MQ=255)
             cgTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtt                                                                                                                               <  1:369296/148‑1 (MQ=255)
              gTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAttt                                                                                                                              <  1:1976847/148‑1 (MQ=255)
               tGATGTGCATAATTTATACTTCTTATGCCAGAACATTACGATTATCCCGACGATGCGCAGTTTGCTGGAACAGCCGGATAACGTTATCGATGCGTTCCTCGCGCCTGGTCACGACAGTATGGTTATAGGCACCGACGCCTATAAtttt                                                                                                                             >  2:1056853/1‑148 (MQ=255)
               tGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATaa                                                                                                                                 >  2:1155478/1‑144 (MQ=255)
               tGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtttt                                                                                                                             <  2:3096996/148‑1 (MQ=255)
               tGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAAtttt                                                                                                                             >  2:1057430/1‑148 (MQ=255)
                   gtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                         >  2:1096941/1‑148 (MQ=255)
                   gtgCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCg                                                                                                                         >  2:2853614/1‑148 (MQ=255)
                     gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                       <  2:145817/148‑1 (MQ=255)
                     gCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGcc                                                                                                                       <  2:2421385/148‑1 (MQ=255)
                        gAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGc                                                                                                                    >  1:1004157/1‑148 (MQ=255)
                         aaTTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCg                                                                                                                   >  1:2800165/1‑148 (MQ=255)
                           tttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAt                                                                                                                 <  1:3814358/148‑1 (MQ=255)
                           tttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAt                                                                                                                 <  1:3223908/148‑1 (MQ=255)
                            ttttACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGAtt                                                                                                                >  1:1779881/1‑148 (MQ=255)
                               tACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             >  1:2638445/1‑148 (MQ=255)
                               tACTTCGTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTc                                                                                                             <  2:1023173/148‑1 (MQ=255)
                                aCTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCa                                                                                                            >  1:1025455/1‑148 (MQ=255)
                                 cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  2:2325220/148‑1 (MQ=255)
                                 cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           >  1:2078699/1‑148 (MQ=255)
                                 cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           <  1:1825655/148‑1 (MQ=255)
                                 cttcttCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCAt                                                                                                           >  1:2078800/1‑148 (MQ=255)
                                     ttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTc                                                                                                       <  1:3515698/148‑1 (MQ=255)
                                     ttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTc                                                                                                       <  1:1266882/148‑1 (MQ=255)
                                     ttctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTc                                                                                                       <  1:593114/148‑1 (MQ=255)
                                      tctGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTcc                                                                                                      >  2:3058175/1‑148 (MQ=255)
                                          ccAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtg                                                                                                  <  1:228071/148‑1 (MQ=255)
                                             gCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtg                                                                                               >  1:1510074/1‑148 (MQ=255)
                                              cacaTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCtggtgg                                                                                              >  2:2259764/1‑148 (MQ=255)
                                                 aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTg                                                                                           >  2:1015048/1‑148 (MQ=255)
                                                 aTTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTg                                                                                           <  2:3380443/148‑1 (MQ=255)
                                                  ttACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACCGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGc                                                                                          >  2:1447253/1‑148 (MQ=255)
                                                  ttACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGc                                                                                          <  1:773669/148‑1 (MQ=255)
                                                   tACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCt                                                                                         >  1:1987771/1‑148 (MQ=255)
                                                   tACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCt                                                                                         >  1:774414/1‑148 (MQ=255)
                                                   tACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCt                                                                                         >  1:1661091/1‑148 (MQ=255)
                                                    aCGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTg                                                                                        >  1:1919170/1‑148 (MQ=255)
                                                         tATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTc                                                                                   >  1:642474/1‑148 (MQ=255)
                                                               gACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAccc                                                                             <  1:1049898/148‑1 (MQ=255)
                                                                aCGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcccc                                                                            <  2:1285231/148‑1 (MQ=255)
                                                                aCGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcccc                                                                            <  1:581984/148‑1 (MQ=255)
                                                                aCGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAAcccc                                                                            <  2:176135/148‑1 (MQ=255)
                                                                     gcgcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGGTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGAt                                                                       <  2:3217717/148‑1 (MQ=255)
                                                                      cgcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATc                                                                      <  2:612965/148‑1 (MQ=255)
                                                                      cgcAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATc                                                                      <  2:1374953/148‑1 (MQ=255)
                                                                        cAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATcta                                                                    <  2:1895484/148‑1 (MQ=255)
                                                                         aGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctac                                                                   <  1:2146065/148‑1 (MQ=255)
                                                                           tttGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATctacta                                                                 <  1:2348571/148‑1 (MQ=255)
                                                                              gCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACaa                                                              <  1:2968441/148‑1 (MQ=255)
                                                                                tGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAgg                                                            <  2:761165/148‑1 (MQ=255)
                                                                                 ggAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGc                                                           <  1:2663278/148‑1 (MQ=255)
                                                                                   aaCAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGt                                                         >  1:3258421/1‑148 (MQ=255)
                                                                                    aCAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTg                                                        <  1:2901549/148‑1 (MQ=255)
                                                                                     cAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCTCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAACGCGTgt                                                       >  2:3543632/1‑147 (MQ=255)
                                                                                      aGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGCGTCACCACACAAAGGAAAAACCCACCCACCCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGt                                                      <  1:862503/148‑1 (MQ=255)
                                                                                       gCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTc                                                     <  2:499199/148‑1 (MQ=255)
                                                                                            aTAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCt                                                >  1:3765963/1‑148 (MQ=255)
                                                                                            aTAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCt                                                >  1:3765396/1‑148 (MQ=255)
                                                                                              aaCGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTgg                                              <  1:270650/148‑1 (MQ=255)
                                                                                               aCGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGt                                             <  1:2290203/148‑1 (MQ=255)
                                                                                                   tATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTgcag                                         >  2:3161089/1‑148 (MQ=255)
                                                                                                         tGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaa                                   <  2:89172/148‑1 (MQ=255)
                                                                                                         tGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaa                                   >  2:181261/1‑148 (MQ=255)
                                                                                                         tGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGaaa                                   >  2:164178/1‑148 (MQ=255)
                                                                                                           cGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAAt                                 <  1:1663444/148‑1 (MQ=255)
                                                                                                             ttCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAg                               <  1:936809/148‑1 (MQ=255)
                                                                                                             ttCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAg                               <  2:1650887/148‑1 (MQ=255)
                                                                                                             ttCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAg                               >  2:1328410/1‑148 (MQ=255)
                                                                                                              tCCTCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGc                              >  2:683518/1‑148 (MQ=255)
                                                                                                                 tCGCGCCGGGTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGc                           >  2:2044714/1‑148 (MQ=255)
                                                                                                                         ggTCACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCa                   <  1:1479278/148‑1 (MQ=255)
                                                                                                                            cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                >  1:1316240/1‑148 (MQ=255)
                                                                                                                            cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                >  1:1316376/1‑148 (MQ=255)
                                                                                                                            cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                >  2:3735116/1‑148 (MQ=255)
                                                                                                                            cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                <  2:2078654/148‑1 (MQ=255)
                                                                                                                            cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                <  2:1004157/148‑1 (MQ=255)
                                                                                                                            cACGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAgg                <  2:2078800/148‑1 (MQ=255)
                                                                                                                             aCGTCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGt               <  2:1568141/148‑1 (MQ=255)
                                                                                                                                tCAGTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTaga            <  2:1113426/148‑1 (MQ=255)
                                                                                                                                   gTATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGaa         <  2:1252605/148‑1 (MQ=255)
                                                                                                                                    tATGGTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAAt        >  2:549689/1‑148 (MQ=255)
                                                                                                                                       ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     >  2:2976930/1‑148 (MQ=255)
                                                                                                                                       ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     >  1:1420907/1‑148 (MQ=255)
                                                                                                                                       ggTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAg     >  1:1149653/1‑148 (MQ=255)
                                                                                                                                        gTTATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGt    <  2:2641066/148‑1 (MQ=255)
                                                                                                                                         ttATAGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTa   >  1:1603803/1‑148 (MQ=255)
                                                                                                                                          tataGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAt  <  2:1485401/148‑1 (MQ=255)
                                                                                                                                          tataGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAt  <  2:1497673/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
ACAGGCGAAAGCGCGTGATGTGCAGAATTTTTACTTCTTCTGCCAGCACATTACGCTTATCCCGACGTTGCGCAGTTTGCTGGAACAGCCGGATAACGGTATCGATGCGTTCCTCGCGCCGGGTCACGTCAGTATGGTTATCGGCACCGACGCCTATAATTTTATCGCCAGCGATTTTCATCGTCCGCTGGTGGTTGCTGGATTCGAACCCCTTGATCTACTACAAGGCGTGGTCATGCTGGTGCAGCAGAAAATAGCGGCCCACAGCAAGGTAGAGAATCAGTAT  >  CP009273/2845971‑2846256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑