Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,876,760 C→T 100% G246G (GGG→GGA)  casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,876,7600CT98.4% 814.6 / 1.8 248G246G (GGG→GGA) casACRISP RNA (crRNA) containing Cascade antiviral complex protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (112/132);  minor base A (2/2);  total (114/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 250 total aligned reads displayed.

CCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAT  >  CP009273/2876615‑2876904
                                                                                                                                                 |                                                                                                                                                
 cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCa                                                                                                                                               >  2:884669/1‑148 (MQ=255)
 cTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCa                                                                                                                                               <  2:1484559/148‑1 (MQ=255)
  tCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCaa                                                                                                                                              <  2:2764051/148‑1 (MQ=255)
  tCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCaa                                                                                                                                              <  2:1346749/148‑1 (MQ=255)
   cAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAAt                                                                                                                                             >  1:3433251/1‑148 (MQ=255)
     aCCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATgg                                                                                                                                           >  2:382327/1‑148 (MQ=255)
       cTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGa                                                                                                                                         <  2:597734/148‑1 (MQ=255)
         ccccTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATc                                                                                                                                       >  2:1272384/1‑148 (MQ=255)
         ccccTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATc                                                                                                                                       >  1:2367182/1‑148 (MQ=255)
          cccTTTCGTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCg                                                                                                                                      <  2:3735498/148‑1 (MQ=255)
             tttCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCAt                                                                                                                                   <  2:598033/148‑1 (MQ=255)
              ttCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATa                                                                                                                                  <  2:352560/148‑1 (MQ=255)
               tCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATaa                                                                                                                                 <  2:3606817/148‑1 (MQ=255)
               tCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATaa                                                                                                                                 <  2:2971438/148‑1 (MQ=255)
                cTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAAt                                                                                                                                >  1:634777/1‑148 (MQ=255)
                  tGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTc                                                                                                                              >  1:107857/1‑148 (MQ=255)
                   gACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCa                                                                                                                             <  1:1432690/148‑1 (MQ=255)
                    aCTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCaa                                                                                                                            >  2:1926920/1‑148 (MQ=255)
                     cTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAat                                                                                                                           <  1:990178/148‑1 (MQ=255)
                      tGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAata                                                                                                                          >  2:2103209/1‑148 (MQ=255)
                           ccAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                     <  1:2751953/148‑1 (MQ=255)
                           ccAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATgcgc                                                                                                                     <  1:2752024/148‑1 (MQ=255)
                            cAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCt                                                                                                                    <  2:3293485/148‑1 (MQ=255)
                               aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                 >  1:899364/1‑148 (MQ=255)
                               aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                 <  1:3606241/148‑1 (MQ=255)
                               aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                 >  1:905743/1‑148 (MQ=255)
                               aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                 >  1:2813696/1‑148 (MQ=255)
                               aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                 <  1:2948554/148‑1 (MQ=255)
                               aCAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATACCAATGGGATCGCATAATTCAATATGCGCTGGt                                                                                                                 >  2:2338971/1‑148 (MQ=255)
                                  aGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGc                                                                                                              <  2:2463553/148‑1 (MQ=255)
                                   ggggAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGcc                                                                                                             <  1:1272384/148‑1 (MQ=255)
                                    gggAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCa                                                                                                            >  1:625833/1‑148 (MQ=255)
                                    gggAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCa                                                                                                            >  2:156052/1‑148 (MQ=255)
                                      gAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGa                                                                                                          <  2:2367182/148‑1 (MQ=255)
                                      gAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGa                                                                                                          >  2:3284445/1‑148 (MQ=255)
                                        aTGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAAt                                                                                                        >  1:2120839/1‑148 (MQ=255)
                                          gCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAg                                                                                                      <  2:3103980/148‑1 (MQ=255)
                                            ggATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGAc                                                                                                    <  2:2150291/148‑1 (MQ=255)
                                                      aTAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACaaa                                                                                          <  1:1654364/148‑1 (MQ=255)
                                                       tAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAc                                                                                         >  1:592736/1‑148 (MQ=255)
                                                        aGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAAcc                                                                                        <  1:1263028/148‑1 (MQ=255)
                                                          cccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCa                                                                                      >  2:955586/1‑148 (MQ=255)
                                                          cccATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCa                                                                                      <  2:1504178/148‑1 (MQ=255)
                                                             aTTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAAtt                                                                                   >  1:3532089/1‑148 (MQ=255)
                                                              ttAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTg                                                                                  <  1:2501337/148‑1 (MQ=255)
                                                               tAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGa                                                                                 >  1:834370/1‑148 (MQ=255)
                                                                 aCTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACg                                                                               <  2:1989806/148‑1 (MQ=255)
                                                                  cTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGa                                                                              <  2:107857/148‑1 (MQ=255)
                                                                   tGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGaa                                                                             >  1:2942509/1‑148 (MQ=255)
                                                                     tAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGc                                                                           <  1:430332/148‑1 (MQ=255)
                                                                     tAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGc                                                                           >  1:3045162/1‑148 (MQ=255)
                                                                      aaaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCa                                                                          >  1:1027394/1‑148 (MQ=255)
                                                                       aaGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAg                                                                         >  1:521215/1‑148 (MQ=255)
                                                                        aGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAgg                                                                        <  1:1469620/148‑1 (MQ=255)
                                                                          gTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGta                                                                      >  2:1649276/1‑148 (MQ=255)
                                                                          gTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGta                                                                      >  1:3081391/1‑148 (MQ=255)
                                                                           tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGtat                                                                     >  1:2079285/1‑148 (MQ=255)
                                                                           tAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGtat                                                                     <  1:3749437/148‑1 (MQ=255)
                                                                             aaTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAg                                                                   <  2:2918792/148‑1 (MQ=255)
                                                                              aTTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGa                                                                  >  1:956656/1‑148 (MQ=255)
                                                                              aTTTTTCCTTAAGAAAACCGGTAGAACGCAAATTGCGTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGa                                                                  <  2:3122995/148‑1 (MQ=255)
                                                                                ttttCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACt                                                                >  1:3212882/1‑148 (MQ=255)
                                                                                   tCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCAt                                                             >  2:1688127/1‑148 (MQ=255)
                                                                                   tCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCAt                                                             <  1:2257094/148‑1 (MQ=255)
                                                                                   tCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCAt                                                             <  1:331806/148‑1 (MQ=255)
                                                                                    ccTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCAtt                                                            <  2:3268235/148‑1 (MQ=255)
                                                                                      ttAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTgg                                                          >  2:401692/1‑148 (MQ=255)
                                                                                       tAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGa                                                         <  2:3707066/148‑1 (MQ=255)
                                                                                          gAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACtt                                                      >  1:2785393/1‑148 (MQ=255)
                                                                                           agaaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTg                                                     >  1:677724/3‑148 (MQ=255)
                                                                                           aaaaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTg                                                     >  1:2699889/1‑148 (MQ=255)
                                                                                           aaaaCCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTg                                                     <  1:3805176/148‑1 (MQ=255)
                                                                                                cGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAgg                                                <  2:806858/148‑1 (MQ=255)
                                                                                                 ggTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGt                                               <  2:656567/148‑1 (MQ=255)
                                                                                                   tataACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGttt                                             >  1:3176396/1‑148 (MQ=255)
                                                                                                       aCGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATc                                         >  2:1302336/1‑148 (MQ=255)
                                                                                                        cGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATcc                                        >  1:2406054/1‑148 (MQ=255)
                                                                                                            aaTTGCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAGTTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggt                                    >  2:260905/1‑148 (MQ=255)
                                                                                                                gCTTTCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCaggtaggt                                <  2:3698189/148‑1 (MQ=255)
                                                                                                                  tttCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggttg                              >  1:2197370/1‑148 (MQ=255)
                                                                                                                   ttCCTGTCCACAGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAggttgg                             >  2:2696868/1‑148 (MQ=255)
                                                                                                                           cacaGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGt                     >  2:27802/1‑148 (MQ=255)
                                                                                                                           cacaGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGt                     >  2:1955880/1‑148 (MQ=255)
                                                                                                                           cacaGCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGt                     <  1:156052/148‑1 (MQ=255)
                                                                                                                            acaGCAAGAACATTTACCAATACCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTa                    <  1:415222/148‑1 (MQ=255)
                                                                                                                               gCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgt                 >  2:3601084/1‑148 (MQ=255)
                                                                                                                               gCAAGAACATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTAtgt                 >  1:821336/1‑148 (MQ=255)
                                                                                                                                    aaCATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc            <  1:202733/148‑1 (MQ=255)
                                                                                                                                    aaCATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc            <  1:2304819/148‑1 (MQ=255)
                                                                                                                                    aaCATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTc            <  2:2724927/148‑1 (MQ=255)
                                                                                                                                      cATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAt          <  1:1508183/148‑1 (MQ=255)
                                                                                                                                      cATTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAt          <  1:733870/148‑1 (MQ=255)
                                                                                                                                       aTTTACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCAtt         >  1:866709/1‑148 (MQ=255)
                                                                                                                                        tttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTa        <  1:1915330/148‑1 (MQ=255)
                                                                                                                                        tttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTa        <  2:545983/148‑1 (MQ=255)
                                                                                                                                         ttACCAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAg       >  2:3443923/1‑148 (MQ=255)
                                                                                                                                            ccAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGaa    <  1:3284445/148‑1 (MQ=255)
                                                                                                                                            ccAATTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGaa    >  2:32171/1‑148 (MQ=255)
                                                                                                                                              aaTTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAt  <  1:3669304/148‑1 (MQ=255)
                                                                                                                                              aaTTCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAt  <  2:1252553/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCTCAACCTCCCCTTTCTTGACTGTTACCAGACAAGGGGAATGCGGATGGGGCCATAGCCCATTAACTGTAAAGGTAAATTTTTCCTTAAGAAAACCGGTATAACGCAAATTGCTTTCCTGTCCACAGCAAGAACATTTACCAATCCCAATGGGATCGCATAATTCAATATGCGCTGGTTGCCAGAATAGACCACGGACAAACCCAATTGACGAAGCAGGTATAGACTCATTGGACTTGATAGGTTTAATCCAGGTAGGTTGGTTTTCCGTATGTGATTCATTAGGAAAT  >  CP009273/2876615‑2876904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑