Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,881,078 G→T 43.7% G198G (GGC→GGA)  cysH ← phosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,881,0780GT43.7% 199.2 / 264.3 229G198G (GGC→GGA) cysHphosphoadenosine phosphosulfate reductase; PAPS reductase, thioredoxin dependent
Reads supporting (aligned to +/- strand):  ref base G (74/55);  new base T (64/36);  total (138/91)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 234 total aligned reads displayed.

CAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGTTTG  >  CP009273/2880931‑2881224
                                                                                                                                                   |                                                                                                                                                  
cAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAgg                                                                                                                                                    <  1:1447873/148‑1 (MQ=255)
   tatTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCa                                                                                                                                                 >  2:2324136/1‑148 (MQ=255)
   tatTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCa                                                                                                                                                 >  2:2325406/1‑148 (MQ=255)
   tatTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCa                                                                                                                                                 >  2:2518052/1‑148 (MQ=255)
     tttACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATg                                                                                                                                               >  1:993597/1‑148 (MQ=255)
      ttACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGt                                                                                                                                              >  1:1610607/1‑148 (MQ=255)
        aCCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGttt                                                                                                                                            <  1:1363224/148‑1 (MQ=255)
           cTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTg                                                                                                                                         >  2:385768/1‑148 (MQ=255)
           cTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTg                                                                                                                                         >  2:2134446/1‑148 (MQ=255)
            ttCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGc                                                                                                                                        >  1:812695/1‑148 (MQ=255)
             tCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCa                                                                                                                                       >  1:2447042/1‑148 (MQ=255)
             tCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCa                                                                                                                                       >  1:3755571/1‑148 (MQ=255)
             tCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCa                                                                                                                                       >  1:2185/1‑148 (MQ=255)
                 gtAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTa                                                                                                                                   <  1:683162/148‑1 (MQ=255)
                 gtAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGGTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTa                                                                                                                                   <  2:1167952/148‑1 (MQ=255)
                     cccACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGa                                                                                                                               >  1:1703279/1‑148 (MQ=255)
                       cacaTTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGACATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATa                                                                                                                             >  2:3359959/1‑148 (MQ=255)
                        acaTTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTGACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATaa                                                                                                                            <  2:308193/148‑1 (MQ=255)
                          aTTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAAt                                                                                                                          >  1:874898/1‑148 (MQ=255)
                            tCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAg                                                                                                                        <  1:3041280/148‑1 (MQ=255)
                             cccTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGt                                                                                                                       <  2:364003/148‑1 (MQ=255)
                             cccTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGt                                                                                                                       <  1:78778/148‑1 (MQ=255)
                             cccTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGt                                                                                                                       <  1:2822290/148‑1 (MQ=255)
                              ccTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGtt                                                                                                                      <  1:355789/148‑1 (MQ=255)
                               cTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTc                                                                                                                     <  2:3494005/148‑1 (MQ=255)
                                 ttttAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCgg                                                                                                                   >  1:1597601/1‑148 (MQ=255)
                                    tAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTa                                                                                                                <  1:3385962/148‑1 (MQ=255)
                                    tAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTa                                                                                                                >  2:2742961/1‑148 (MQ=255)
                                      aGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATc                                                                                                              >  2:1803673/1‑148 (MQ=255)
                                       gCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATACTGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATcc                                                                                                             >  2:2063901/1‑148 (MQ=255)
                                       gCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATcc                                                                                                             >  1:1500912/1‑148 (MQ=255)
                                        ccAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATccc                                                                                                            >  2:878300/1‑148 (MQ=255)
                                         cAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCa                                                                                                           <  1:2027196/148‑1 (MQ=255)
                                         cAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCa                                                                                                           <  1:3011471/148‑1 (MQ=255)
                                           aaGAACCGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAAGGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGt                                                                                                         >  2:2745176/1‑148 (MQ=255)
                                           aaGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGt                                                                                                         >  1:3410120/1‑148 (MQ=255)
                                            aGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTc                                                                                                        >  2:1377847/1‑148 (MQ=255)
                                             gAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCg                                                                                                       >  2:2051790/1‑148 (MQ=255)
                                                aCGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATa                                                                                                    <  1:3719438/148‑1 (MQ=255)
                                                 cgcgTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATaa                                                                                                   <  1:175446/148‑1 (MQ=255)
                                                      ttcttcttcCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGgc                                                                                              <  2:1912870/148‑1 (MQ=255)
                                                        cttcttcCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGgcag                                                                                            >  2:3780922/1‑148 (MQ=255)
                                                         ttcttcCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGgcagc                                                                                           >  2:2646467/1‑148 (MQ=255)
                                                           cttcCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCAc                                                                                         <  2:2081884/148‑1 (MQ=255)
                                                            ttcCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACt                                                                                        <  1:267260/148‑1 (MQ=255)
                                                            ttcCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACt                                                                                        <  1:268554/148‑1 (MQ=255)
                                                                gCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTaa                                                                                    <  1:1456366/148‑1 (MQ=255)
                                                                  cATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAAt                                                                                  <  2:1273297/148‑1 (MQ=255)
                                                                         ggTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCAc                                                                           >  1:972616/1‑148 (MQ=255)
                                                                         ggTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATACCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCAc                                                                           >  2:3091519/1‑148 (MQ=255)
                                                                             cccATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTg                                                                       >  2:669656/1‑148 (MQ=255)
                                                                                aTTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAAt                                                                    >  2:1673179/1‑148 (MQ=255)
                                                                                       ggTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGc                                                             >  2:1296825/1‑148 (MQ=255)
                                                                                         ttGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCAc                                                           >  1:3300754/1‑148 (MQ=255)
                                                                                         ttGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCACGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCAc                                                           >  1:2410798/1‑148 (MQ=255)
                                                                                             aTGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGt                                                       <  1:3242876/148‑1 (MQ=255)
                                                                                             aTGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGTAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGt                                                       <  2:589315/148‑1 (MQ=255)
                                                                                               gggTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTaa                                                     <  1:1043893/148‑1 (MQ=255)
                                                                                               gggTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTaa                                                     <  2:2940105/148‑1 (MQ=255)
                                                                                               gggTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTaa                                                     >  1:2783296/1‑148 (MQ=255)
                                                                                               gggTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTaa                                                     <  1:3488388/148‑1 (MQ=255)
                                                                                               gggTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTaa                                                     <  1:3579460/148‑1 (MQ=255)
                                                                                                 gTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAAt                                                   <  2:511023/148‑1 (MQ=255)
                                                                                                 gTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAAt                                                   <  2:2863761/148‑1 (MQ=255)
                                                                                                 gTATCACCGACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAAt                                                   <  1:1272126/148‑1 (MQ=255)
                                                                                                        ccACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCATCACCGGTAAATTGGCACg                                            >  1:1242547/1‑148 (MQ=255)
                                                                                                         cACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGa                                           <  1:3526011/148‑1 (MQ=255)
                                                                                                            cGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTg                                        <  1:1712010/148‑1 (MQ=255)
                                                                                                               tAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCg                                     >  1:569851/1‑148 (MQ=255)
                                                                                                               tAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCg                                     >  1:2925785/1‑148 (MQ=255)
                                                                                                               tAAATATCCTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCg                                     >  1:1858261/1‑148 (MQ=255)
                                                                                                                    atCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTg                                >  1:3022523/1‑148 (MQ=255)
                                                                                                                      ccTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGtt                              <  1:49637/148‑1 (MQ=255)
                                                                                                                      ccTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGtt                              >  2:3725531/1‑148 (MQ=255)
                                                                                                                      ccTTCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGtt                              >  2:1834438/1‑148 (MQ=255)
                                                                                                                        ttCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCa                            >  2:2152546/1‑148 (MQ=255)
                                                                                                                         tCATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCAc                           >  1:2352919/1‑148 (MQ=255)
                                                                                                                         tCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCAc                           <  1:2560512/148‑1 (MQ=255)
                                                                                                                         tCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCAc                           >  2:3552480/1‑148 (MQ=255)
                                                                                                                         tCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCAc                           <  1:641342/148‑1 (MQ=255)
                                                                                                                          cATCCCATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACg                          <  1:2447674/148‑1 (MQ=255)
                                                                                                                          cATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACg                          <  1:2020343/148‑1 (MQ=255)
                                                                                                                           aTCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACgg                         >  2:1226259/1‑148 (MQ=255)
                                                                                                                               cATAATGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCa                     >  2:665384/1‑148 (MQ=255)
                                                                                                                               cATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCa                     <  1:2277455/148‑1 (MQ=255)
                                                                                                                                aTAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAg                    >  2:782690/1‑148 (MQ=255)
                                                                                                                                  aaTGGGTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGc                  >  2:2536735/1‑148 (MQ=255)
                                                                                                                                  aaTGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGc                  >  2:1115864/1‑148 (MQ=255)
                                                                                                                                     gggTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAg               >  2:3224006/1‑148 (MQ=255)
                                                                                                                                     gggTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAg               >  2:764048/1‑148 (MQ=255)
                                                                                                                                      ggTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGc              >  2:2521345/1‑148 (MQ=255)
                                                                                                                                       gTGATATTTCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCa             >  2:906735/1‑148 (MQ=255)
                                                                                                                                         gATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACAGCCGGATTGTTCACGGCGCAGGCCAGCaaa           >  1:3374542/1‑148 (MQ=255)
                                                                                                                                           tatTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAAcc         <  1:557819/148‑1 (MQ=255)
                                                                                                                                           tatTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAAcc         <  1:1138522/148‑1 (MQ=255)
                                                                                                                                              ttCAGTCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAgg      >  1:1925833/1‑148 (MQ=255)
                                                                                                                                              ttCAGGCCATGTTTTTGCTGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAgg      <  1:320989/148‑1 (MQ=255)
                                                                                                                                              ttCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAgg      >  2:362993/1‑148 (MQ=255)
                                                                                                                                               tCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGt     <  1:957393/148‑1 (MQ=255)
                                                                                                                                               tCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGt     <  1:1093256/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGAAACGCGTTTCTTCTTCCGCCATGCCGGGTTCCCATTTACGGGTTGTATGGGTATCGCCCACCGATAAATATCCTTCATCCCATAATGGGTGATATTTCAGGCCATGTTTTTGCAGGTACTGATAAATAGTTCGGTTATCCCAGTCGATAATCGGCAGCACTTTAAATACGCCACGCTGAATTGCCAGCACCGGTAAATTGGCACGACTGCCGGATTGTTCACGGCGCAGGCCAGCAAACCAGGTTTG  >  CP009273/2880931‑2881224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑