Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,901,514 C→A 15.8% P504P (CCG→CCT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,901,5140CA15.8% 577.3 / 56.4 189P504P (CCG→CCT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (69/89);  new base A (13/17);  total (83/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

TTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTG  >  CP009273/2901367‑2901660
                                                                                                                                                   |                                                                                                                                                  
ttGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTc                                                                                                                                                    >  2:1089615/1‑148 (MQ=255)
 tGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCCTCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCg                                                                                                                                                   >  1:1455586/1‑148 (MQ=255)
   ccGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGa                                                                                                                                                 >  1:1092156/1‑148 (MQ=255)
    cGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGaa                                                                                                                                                <  1:2276207/148‑1 (MQ=255)
     gCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAAc                                                                                                                                               <  2:639787/148‑1 (MQ=255)
      ctctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCAACACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACt                                                                                                                                              >  1:311040/1‑148 (MQ=255)
       tctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACtt                                                                                                                                             <  1:1901701/148‑1 (MQ=255)
       tctAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACtt                                                                                                                                             <  1:1590070/148‑1 (MQ=255)
         tAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCg                                                                                                                                           <  1:1485837/148‑1 (MQ=255)
         tAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCg                                                                                                                                           <  2:503158/148‑1 (MQ=255)
         tAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCg                                                                                                                                           <  2:2067179/148‑1 (MQ=255)
          aaCTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCga                                                                                                                                          <  1:2190910/148‑1 (MQ=255)
            cTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatg                                                                                                                                        >  2:1647616/1‑148 (MQ=255)
            cTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatg                                                                                                                                        <  1:2384302/148‑1 (MQ=255)
            cTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCgatg                                                                                                                                        <  2:2766858/148‑1 (MQ=255)
             ttttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatga                                                                                                                                       <  1:2595603/148‑1 (MQ=255)
              tttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCgatgat                                                                                                                                      >  1:953790/1‑148 (MQ=255)
               ttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGAtc                                                                                                                                     >  1:3023029/1‑148 (MQ=255)
               ttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGAtc                                                                                                                                     >  1:3417310/1‑148 (MQ=255)
               ttttACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGAtc                                                                                                                                     <  1:947405/148‑1 (MQ=255)
                    cttacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGAc                                                                                                                                <  1:3212350/148‑1 (MQ=255)
                      tacttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCa                                                                                                                              >  2:2744262/1‑148 (MQ=255)
                       acttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCaa                                                                                                                             <  2:2909794/148‑1 (MQ=255)
                        cttCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAAc                                                                                                                            >  1:1719767/1‑148 (MQ=255)
                           cGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGa                                                                                                                         <  1:722371/148‑1 (MQ=255)
                                gACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGatcatc                                                                                                                    <  1:3429483/148‑1 (MQ=255)
                                gACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGatcatc                                                                                                                    >  1:1828005/1‑148 (MQ=255)
                                       cTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGaa                                                                                                             <  1:2486693/148‑1 (MQ=255)
                                          gAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACgg                                                                                                          >  1:3745512/1‑148 (MQ=255)
                                             cTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTTGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGACCGGccc                                                                                                       >  2:604857/1‑148 (MQ=255)
                                             cTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGccc                                                                                                       >  2:2187790/1‑148 (MQ=255)
                                                 cTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCaa                                                                                                   <  2:876257/148‑1 (MQ=255)
                                                   ggcggcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAAcg                                                                                                 <  1:675392/148‑1 (MQ=255)
                                                    gcggcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAAcgc                                                                                                >  2:3208907/1‑148 (MQ=255)
                                                     cggcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAAcgcg                                                                                               >  1:1188513/1‑148 (MQ=255)
                                                      ggcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACgcgc                                                                                              <  1:2431152/148‑1 (MQ=255)
                                                       gcTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCa                                                                                             >  1:1018715/1‑148 (MQ=255)
                                                         tttCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGa                                                                                           <  1:876638/148‑1 (MQ=255)
                                                          ttCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGAc                                                                                          <  1:242127/148‑1 (MQ=255)
                                                                aaGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCAt                                                                                    <  1:2711965/148‑1 (MQ=255)
                                                                aaGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCAt                                                                                    >  2:102678/1‑148 (MQ=255)
                                                                 aGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATc                                                                                   <  2:1105207/148‑1 (MQ=255)
                                                                 aGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATc                                                                                   >  1:1293049/1‑148 (MQ=255)
                                                                 aGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATc                                                                                   >  2:2985230/1‑148 (MQ=255)
                                                                 aGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATc                                                                                   <  2:1912596/148‑1 (MQ=255)
                                                                   ccTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCtt                                                                                 >  2:980608/1‑148 (MQ=255)
                                                                     tGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCa                                                                               <  1:1562194/148‑1 (MQ=255)
                                                                       cAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAAt                                                                             <  2:933383/148‑1 (MQ=255)
                                                                       cAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAAt                                                                             <  2:3819947/148‑1 (MQ=255)
                                                                          aCAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTg                                                                          >  1:2013628/1‑148 (MQ=255)
                                                                           cAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGt                                                                         <  2:2641813/148‑1 (MQ=255)
                                                                           cAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGt                                                                         <  1:989352/148‑1 (MQ=255)
                                                                            aGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGtt                                                                        >  1:1516574/1‑148 (MQ=255)
                                                                            aGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGtt                                                                        >  1:1517588/1‑148 (MQ=255)
                                                                            aGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGtt                                                                        <  2:3815704/148‑1 (MQ=255)
                                                                              cGGGTGACCATCACGTCGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCACCTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTc                                                                      >  1:1636117/1‑148 (MQ=255)
                                                                               gggTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCa                                                                     <  1:3643923/148‑1 (MQ=255)
                                                                                ggTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTTATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCaa                                                                    <  1:2887732/148‑1 (MQ=255)
                                                                                     ccATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCa                                                               <  1:1041299/148‑1 (MQ=255)
                                                                                       aTCACGTGGAGTAGAAGTAAACGCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtg                                                             <  2:3375524/148‑1 (MQ=255)
                                                                                         cACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtgtt                                                           >  1:361192/1‑148 (MQ=255)
                                                                                          aCGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCAtgttg                                                          <  1:110834/148‑1 (MQ=255)
                                                                                                aGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACt                                                    <  1:1860087/148‑1 (MQ=255)
                                                                                                   aGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCg                                                 >  1:2364182/1‑148 (MQ=255)
                                                                                                     aaGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTa                                               >  2:2354931/1‑148 (MQ=255)
                                                                                                         aaaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACgg                                           >  2:571451/1‑148 (MQ=255)
                                                                                                         aaaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACgg                                           <  1:2296455/148‑1 (MQ=255)
                                                                                                          aaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGt                                          >  1:380663/1‑148 (MQ=255)
                                                                                                          aaCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGt                                          >  1:377811/1‑148 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         <  1:3201265/148‑1 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         <  1:74338/148‑1 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         >  2:843574/1‑148 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         >  2:1922190/1‑148 (MQ=255)
                                                                                                           aCTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTg                                         >  1:321772/1‑148 (MQ=255)
                                                                                                            cTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGTCCTGCATCTTCAATCTGTTTCAACAGCATGTTTTTGACTTCGTAACGGTGa                                        <  2:311040/148‑1 (MQ=255)
                                                                                                              ccGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACg                                      <  2:3714704/148‑1 (MQ=255)
                                                                                                              ccGGATGGAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACg                                      <  1:2135761/148‑1 (MQ=255)
                                                                                                                    ggAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACg                                <  2:3505990/148‑1 (MQ=255)
                                                                                                                    ggAACTGGCAAGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACg                                >  2:512883/1‑148 (MQ=255)
                                                                                                                     gAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGc                               <  1:2650543/148‑1 (MQ=255)
                                                                                                                      aaCTGGCACGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCt                              <  1:2933934/148‑1 (MQ=255)
                                                                                                                      aaCTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCt                              >  2:2654978/1‑148 (MQ=255)
                                                                                                                      aaCTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCt                              <  1:2933911/148‑1 (MQ=255)
                                                                                                                         tGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaa                           >  1:2855929/1‑148 (MQ=255)
                                                                                                                         tGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaa                           >  1:2855994/1‑148 (MQ=255)
                                                                                                                         tGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaa                           >  1:57060/1‑148 (MQ=255)
                                                                                                                            cAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTcaacaa                        <  1:13873/148‑1 (MQ=255)
                                                                                                                             aaGCCACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAAt                       >  2:1746416/1‑148 (MQ=255)
                                                                                                                              aGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAAtt                      >  2:3441996/1‑148 (MQ=255)
                                                                                                                              aGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAAtt                      <  1:2723739/148‑1 (MQ=255)
                                                                                                                              aGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAAtt                      <  1:2724026/148‑1 (MQ=255)
                                                                                                                                ccACGAACCACGGGTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGt                    <  1:1550002/148‑1 (MQ=255)
                                                                                                                                 cACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGACCGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTc                   >  1:3040608/1‑148 (MQ=255)
                                                                                                                                    gAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGgc                >  2:1357442/1‑148 (MQ=255)
                                                                                                                                     aaCCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGgcg               <  1:3188240/148‑1 (MQ=255)
                                                                                                                                          cGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTg          >  2:3058664/1‑148 (MQ=255)
                                                                                                                                            ggTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTa        <  2:860587/148‑1 (MQ=255)
                                                                                                                                             gTGATTAGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTAc       <  2:728204/148‑1 (MQ=255)
                                                                                                                                               gATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAg     <  2:1475502/148‑1 (MQ=255)
                                                                                                                                               gATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGAATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAg     <  1:834670/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACCACGGGTGATTCGGAACTTCGATGATCTCGACCAACTGATCATCCCCGGAACGGCCCGCAACGCGCAGACCTGCATCTTCAATCTGTTTCAACAGCATGTTGTTGACTTCGTAACGGTGACGATGACGCTCAACAATTGTCGGCGCATTGTACAGCTG  >  CP009273/2901367‑2901660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑