Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,902,302 C→A 44.7% D242Y (GAT→TAT)  pyrG ← CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,902,3020CA44.7% 165.5 / 246.8 208D242Y (GAT→TAT) pyrGCTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (59/56);  new base A (50/43);  total (109/99)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 208 total aligned reads displayed.

GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGT  >  CP009273/2902156‑2902448
                                                                                                                                                  |                                                                                                                                                  
 aCTTCACTTACCGGGTTCGCTTCTTCTAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGa                                                                                                                                                  <  1:455594/148‑1 (MQ=255)
 aCTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGa                                                                                                                                                  <  1:858536/148‑1 (MQ=255)
  cTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGAc                                                                                                                                                 >  1:2415666/1‑148 (MQ=255)
   ttCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACg                                                                                                                                                >  1:2300851/1‑148 (MQ=255)
    tCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGt                                                                                                                                               >  2:825736/1‑148 (MQ=255)
      aCTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCt                                                                                                                                             >  1:2826810/1‑148 (MQ=255)
       cTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCtt                                                                                                                                            >  2:2342531/1‑148 (MQ=255)
        ttACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCttt                                                                                                                                           <  1:2076246/148‑1 (MQ=255)
          aCCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCa                                                                                                                                         <  1:2720524/148‑1 (MQ=255)
           ccGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCag                                                                                                                                        >  1:3450873/1‑148 (MQ=255)
           ccGGGTGCGCTTCTTCGAAGATAACCTGTTCCCAGTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCag                                                                                                                                        <  1:2755942/148‑1 (MQ=255)
              ggTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATAGTTTACAAATATAATAGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATATATGGAATATACGTCTTTAAgaga                                                                                                                                     >  1:1076632/1‑148 (MQ=255)
               gTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGaa                                                                                                                                    <  2:1952066/148‑1 (MQ=255)
                ttCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTAAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGaaa                                                                                                                                   >  1:1668886/1‑148 (MQ=255)
                 tCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAAt                                                                                                                                  >  1:1108608/1‑148 (MQ=255)
                  cGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATa                                                                                                                                 <  2:1311388/148‑1 (MQ=255)
                   gCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATaa                                                                                                                                <  1:825736/148‑1 (MQ=255)
                    cttcttCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAc                                                                                                                               >  1:752234/1‑148 (MQ=255)
                    cttcttCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAAc                                                                                                                               >  1:1020509/1‑148 (MQ=255)
                       cttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGc                                                                                                                            <  2:3738016/148‑1 (MQ=255)
                        ttcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCt                                                                                                                           <  2:2690611/148‑1 (MQ=255)
                         tcGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCtt                                                                                                                          <  2:535175/148‑1 (MQ=255)
                               aTAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCg                                                                                                                    <  1:498001/148‑1 (MQ=255)
                                    cTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACa                                                                                                               <  1:2912531/148‑1 (MQ=255)
                                     tGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAt                                                                                                              >  1:1217151/1‑148 (MQ=255)
                                      gTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAtt                                                                                                             >  2:2712663/1‑148 (MQ=255)
                                      gTTCCCATTCGGACAGATTCGCGTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACAtt                                                                                                             <  2:1184495/148‑1 (MQ=255)
                                        tCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATACTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTAc                                                                                                           <  1:249955/148‑1 (MQ=255)
                                          ccATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAg                                                                                                         >  2:335087/1‑148 (MQ=255)
                                           cATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGa                                                                                                        <  1:1499179/148‑1 (MQ=255)
                                             ttCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAAc                                                                                                      >  1:1575365/1‑148 (MQ=255)
                                              tCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACa                                                                                                     >  1:2349486/1‑148 (MQ=255)
                                               cGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACaa                                                                                                    <  2:1739284/148‑1 (MQ=255)
                                                ggACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAAt                                                                                                   <  1:2114187/148‑1 (MQ=255)
                                                ggACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAAt                                                                                                   <  1:3378418/148‑1 (MQ=255)
                                                 gACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATAGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATg                                                                                                  >  1:117683/1‑148 (MQ=255)
                                                  aCAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGc                                                                                                 >  1:240635/1‑148 (MQ=255)
                                                   cAGATTCGCTTCCGGGCAGTTTAAGCTGAATCTTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCa                                                                                                <  2:1568484/148‑1 (MQ=255)
                                                     gATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAAt                                                                                              <  1:3664687/148‑1 (MQ=255)
                                                      aTTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATc                                                                                             >  1:3098064/1‑148 (MQ=255)
                                                          gCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCg                                                                                         <  2:1795907/148‑1 (MQ=255)
                                                            ttCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCa                                                                                       >  2:1524433/1‑148 (MQ=255)
                                                                   cAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgttcgt                                                                                >  2:2996710/1‑148 (MQ=255)
                                                                   cAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCAcgttcgt                                                                                <  2:3098064/148‑1 (MQ=255)
                                                                       ttAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCCCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGc                                                                            >  1:3549039/1‑148 (MQ=255)
                                                                          aGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCgg                                                                         >  2:3374786/1‑148 (MQ=255)
                                                                           gCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGa                                                                        <  2:3272644/148‑1 (MQ=255)
                                                                               aaTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAg                                                                    >  2:2719431/1‑148 (MQ=255)
                                                                                aTCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgc                                                                   >  2:1304275/1‑148 (MQ=255)
                                                                                 tCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgcg                                                                  >  2:213405/1‑148 (MQ=255)
                                                                                 tCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAgcg                                                                  >  1:943151/1‑148 (MQ=255)
                                                                                     ttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGAt                                                              >  1:3057684/1‑148 (MQ=255)
                                                                                     ttACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGAt                                                              <  1:2999502/148‑1 (MQ=255)
                                                                                      tACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATc                                                             <  1:1506797/148‑1 (MQ=255)
                                                                                        cAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTg                                                           <  1:529265/148‑1 (MQ=255)
                                                                                         aaaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGa                                                          <  2:3652561/148‑1 (MQ=255)
                                                                                         aaaTATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGa                                                          <  2:2075930/148‑1 (MQ=255)
                                                                                           atatAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAAc                                                        >  2:3470719/1‑148 (MQ=255)
                                                                                            tataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGc                                                                            >  1:507700/1‑127 (MQ=255)
                                                                                            tataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGc                                                                            <  2:507700/127‑1 (MQ=255)
                                                                                             ataATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGa                                                      >  2:3624639/1‑148 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                   >  1:1214010/1‑148 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                   >  1:409818/1‑148 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                   >  2:2574900/1‑148 (MQ=255)
                                                                                                aTCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACaa                                                   >  2:3604318/1‑148 (MQ=255)
                                                                                                    tCCAGCCCCTGAGATTTCAACCGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCa                                               >  2:3066637/1‑148 (MQ=255)
                                                                                                     ccAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAg                                              >  1:1341087/1‑148 (MQ=255)
                                                                                                          cccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGt                                         >  2:1496901/1‑148 (MQ=255)
                                                                                                           ccTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTc                                        >  2:304625/1‑148 (MQ=255)
                                                                                                            cTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCa                                       <  2:1132594/148‑1 (MQ=255)
                                                                                                             tGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAg                                      >  1:224440/1‑148 (MQ=255)
                                                                                                                 aTTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTg                                  >  1:1944793/1‑148 (MQ=255)
                                                                                                                   ttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                <  1:1418526/148‑1 (MQ=255)
                                                                                                                   ttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                <  1:2511257/148‑1 (MQ=255)
                                                                                                                   ttCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGATCGATCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGa                                >  2:1053664/1‑148 (MQ=255)
                                                                                                                    tCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGAt                               <  2:3450873/148‑1 (MQ=255)
                                                                                                                       aCAGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATAcc                            >  1:3181869/1‑148 (MQ=255)
                                                                                                                         aGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGa                          <  2:2576778/148‑1 (MQ=255)
                                                                                                                         aGGCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGa                          <  2:3528970/148‑1 (MQ=255)
                                                                                                                           gCCCGGAATTTTATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATg                        <  1:68052/148‑1 (MQ=255)
                                                                                                                                gAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGCTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGagag                   <  2:654930/148‑1 (MQ=255)
                                                                                                                                  aTTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagca                 >  2:576901/1‑148 (MQ=255)
                                                                                                                                  aTTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagca                 >  1:101661/1‑148 (MQ=255)
                                                                                                                                   ttttATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagcag                <  1:1384000/148‑1 (MQ=255)
                                                                                                                                    tttATAGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGagcagc               >  2:3128918/1‑148 (MQ=255)
                                                                                                                                     ttATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGct              <  2:2392784/148‑1 (MQ=255)
                                                                                                                                      tataGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGctc             <  1:421751/148‑1 (MQ=255)
                                                                                                                                       ataGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGGTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGctct            <  2:2862375/148‑1 (MQ=255)
                                                                                                                                        taGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCtt           <  1:3562178/148‑1 (MQ=255)
                                                                                                                                         aGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCttt          <  1:3286831/148‑1 (MQ=255)
                                                                                                                                         aGATGGAATAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCttt          >  2:3195659/1‑148 (MQ=255)
                                                                                                                                           aTGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTa        >  2:253773/1‑148 (MQ=255)
                                                                                                                                            tGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTAc       <  1:3481072/148‑1 (MQ=255)
                                                                                                                                            tGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTAc       <  2:1049277/148‑1 (MQ=255)
                                                                                                                                             ggAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACa      >  2:1409441/1‑148 (MQ=255)
                                                                                                                                              gAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACag     >  1:3377148/1‑148 (MQ=255)
                                                                                                                                               aaTCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACaga    >  2:2177482/1‑148 (MQ=255)
                                                                                                                                               aaTCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACaga    >  1:894402/1‑148 (MQ=255)
                                                                                                                                               aaTCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACaga    >  2:1604659/1‑148 (MQ=255)
                                                                                                                                               aaTAGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACaga    <  2:2826810/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GACTTCACTTACCGGGTTCGCTTCTTCGAAGATAACCTGTTCCCATTCGGACAGATTCGCTTCCGGGCAGTTTAAGCTGAATCGTTTACAAATATAATCGTCCAGCCCCTGAGATTTCAACAGGCCCGGAATTTTATAGATGGAATCGACGTCTTTCAGAGAAATAACCGCTTTTTCCGGAACATTACAGAACAATGCAATCTTCGCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGTCAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGT  >  CP009273/2902156‑2902448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑