Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 236,234 A→T 100% V148E (GTG→GAG)  yafV ← putative NAD(P)‑binding C‑N hydrolase family amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273236,2340AT99.5% 677.5 / ‑4.6 203V148E (GTG→GAG) yafVputative NAD(P)‑binding C‑N hydrolase family amidase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (68/134);  total (69/134)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

GCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGC  >  CP009273/236089‑236378
                                                                                                                                                 |                                                                                                                                                
gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACcactca                                                                                                                                                <  1:1736180/148‑1 (MQ=255)
gCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACcactca                                                                                                                                                >  2:3707369/1‑148 (MQ=255)
     cGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGaaa                                                                                                                                           <  2:1612084/148‑1 (MQ=255)
     cGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGaaa                                                                                                                                           <  2:630790/148‑1 (MQ=255)
      gACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGaaaa                                                                                                                                          <  2:887720/148‑1 (MQ=255)
          cgATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTa                                                                                                                                      >  2:2756243/1‑148 (MQ=255)
             ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGt                                                                                                                                   <  2:1788108/148‑1 (MQ=255)
             ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGt                                                                                                                                   >  2:732778/1‑148 (MQ=255)
             ttGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGt                                                                                                                                   >  2:918415/1‑148 (MQ=255)
                cATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGt                                                                                                                                <  2:282900/148‑1 (MQ=255)
                 aTCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTa                                                                                                                               <  2:2725395/148‑1 (MQ=255)
                   cccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGc                                                                                                                             >  1:2281444/1‑148 (MQ=255)
                       ccACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCacac                                                                                                                         >  1:1548794/1‑148 (MQ=255)
                        cacaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACAcc                                                                                                                        <  2:3464231/148‑1 (MQ=255)
                            tACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCg                                                                                                                    >  1:903232/1‑148 (MQ=255)
                            tACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCg                                                                                                                    >  1:3274989/1‑148 (MQ=255)
                               gCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCa                                                                                                                 <  1:397385/148‑1 (MQ=255)
                                   gATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                             >  2:3410520/1‑148 (MQ=255)
                                   gATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                             <  1:3094834/148‑1 (MQ=255)
                                   gATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                             >  2:2789326/1‑148 (MQ=255)
                                     ttCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATAc                                                                                                           >  2:773150/1‑148 (MQ=255)
                                      tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTTAGTCGTAGCACACCAGCGGCAAAATACg                                                                                                          <  1:195926/148‑1 (MQ=255)
                                      tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACg                                                                                                          <  1:2847119/148‑1 (MQ=255)
                                        tcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGcc                                                                                                        <  2:1235105/148‑1 (MQ=255)
                                            tCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                    >  2:2500472/1‑148 (MQ=255)
                                               cgcgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCcgcg                                                                                                 <  2:1867888/148‑1 (MQ=255)
                                                  gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                              <  2:2368975/148‑1 (MQ=255)
                                                  gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                              <  2:3209726/148‑1 (MQ=255)
                                                     ccGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTc                                                                                           <  1:1753113/148‑1 (MQ=255)
                                                       gTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCca                                                                                         <  1:730754/148‑1 (MQ=255)
                                                         cAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCcaca                                                                                       >  1:1380548/1‑148 (MQ=255)
                                                            cAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATc                                                                                    >  1:3383802/1‑148 (MQ=255)
                                                            cAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATc                                                                                    <  2:549351/148‑1 (MQ=255)
                                                            cAATGCCTGCCAGTCCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATc                                                                                    <  2:652524/148‑1 (MQ=255)
                                                              aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                  >  1:3531912/1‑148 (MQ=255)
                                                                     ccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCa                                                                           <  2:3743916/148‑1 (MQ=255)
                                                                      cAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCAt                                                                          <  2:2280887/148‑1 (MQ=255)
                                                                      cAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCAt                                                                          <  2:3433882/148‑1 (MQ=255)
                                                                      cAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCAt                                                                          <  2:429043/148‑1 (MQ=255)
                                                                         tGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGc                                                                       >  1:45326/1‑148 (MQ=255)
                                                                          gCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGcc                                                                      <  2:2979764/148‑1 (MQ=255)
                                                                             gagagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGc                                                                   >  2:3511969/1‑148 (MQ=255)
                                                                             gagagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGc                                                                   <  1:2538997/148‑1 (MQ=255)
                                                                              agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                  <  1:3205196/148‑1 (MQ=255)
                                                                              agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                  <  2:2559004/148‑1 (MQ=255)
                                                                              agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                  <  1:3208425/148‑1 (MQ=255)
                                                                               gagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCtt                                                                 >  2:3165281/1‑148 (MQ=255)
                                                                               gagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCtt                                                                 >  2:578871/1‑148 (MQ=255)
                                                                                 gcgcGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTta                                                               <  1:3120969/148‑1 (MQ=255)
                                                                                    cgGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATaa                                                            <  1:3511969/148‑1 (MQ=255)
                                                                                     ggAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAAt                                                           <  2:2668039/148‑1 (MQ=255)
                                                                                         cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCTTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCTCTTTATAATGTAg                                                       >  2:2201929/1‑148 (MQ=255)
                                                                                         cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                       <  2:292710/148‑1 (MQ=255)
                                                                                         cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                       <  2:3162297/148‑1 (MQ=255)
                                                                                         cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                       >  2:3594269/1‑148 (MQ=255)
                                                                                          aGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGa                                                      <  1:1138029/148‑1 (MQ=255)
                                                                                          aGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGa                                                      <  1:1944222/148‑1 (MQ=255)
                                                                                            gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                    <  1:3053165/148‑1 (MQ=255)
                                                                                            gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                    <  1:3053082/148‑1 (MQ=255)
                                                                                                gTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCa                                                >  2:636564/1‑148 (MQ=255)
                                                                                                 ttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                               <  2:1660084/148‑1 (MQ=255)
                                                                                                 ttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                               >  1:3626460/1‑148 (MQ=255)
                                                                                                 ttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                               >  2:1047201/1‑148 (MQ=255)
                                                                                                     cGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGc                                           >  2:2813331/1‑148 (MQ=255)
                                                                                                       aCGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCa                                         <  1:269635/148‑1 (MQ=255)
                                                                                                              gggCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGaa                                  >  2:1170205/1‑148 (MQ=255)
                                                                                                              gggCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGaa                                  >  1:2871455/1‑148 (MQ=255)
                                                                                                               ggCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAAc                                 <  2:1269564/148‑1 (MQ=255)
                                                                                                                 cGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAg                               <  1:1630957/148‑1 (MQ=255)
                                                                                                                   aGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                             <  2:2299723/148‑1 (MQ=255)
                                                                                                                   aGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                             >  2:1621718/1‑148 (MQ=255)
                                                                                                                    ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATg                            <  1:2258334/148‑1 (MQ=255)
                                                                                                                    ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATg                            <  1:918459/148‑1 (MQ=255)
                                                                                                                    ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATg                            <  1:804816/148‑1 (MQ=255)
                                                                                                                    ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATg                            <  2:927464/148‑1 (MQ=255)
                                                                                                                     gTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGa                           <  2:903232/148‑1 (MQ=255)
                                                                                                                       cATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACg                         <  2:2594722/148‑1 (MQ=255)
                                                                                                                        aTAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCAGTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGc                        <  2:1091694/148‑1 (MQ=255)
                                                                                                                          aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCtt                      >  2:3494984/1‑148 (MQ=255)
                                                                                                                          aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCtt                      <  1:1468205/148‑1 (MQ=255)
                                                                                                                          aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCtt                      <  1:1413156/148‑1 (MQ=255)
                                                                                                                          aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCtt                      >  2:45374/1‑148 (MQ=255)
                                                                                                                           aTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTa                     >  1:2984216/1‑148 (MQ=255)
                                                                                                                            tCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTAt                    <  2:1448937/148‑1 (MQ=255)
                                                                                                                            tCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTAt                    <  1:2337649/148‑1 (MQ=255)
                                                                                                                             cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATc                   <  2:2227839/148‑1 (MQ=255)
                                                                                                                             cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATc                   <  1:3459550/148‑1 (MQ=255)
                                                                                                                              gTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCa                  <  2:1471707/148‑1 (MQ=255)
                                                                                                                                tGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATa                >  2:1444106/1‑148 (MQ=255)
                                                                                                                                   gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa             <  2:437044/148‑1 (MQ=255)
                                                                                                                                   gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa             >  1:56706/1‑148 (MQ=255)
                                                                                                                                   gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa             >  1:56672/1‑148 (MQ=255)
                                                                                                                                   gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa             >  1:3017125/1‑148 (MQ=255)
                                                                                                                                    aTTGCGCGACCACTCATGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCTCATTGCCCGATTTATTATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaaa            <  1:3103547/148‑1 (MQ=255)
                                                                                                                                    aTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaaa            <  1:1198463/148‑1 (MQ=255)
                                                                                                                                        cgcgACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTa        >  2:1810579/1‑148 (MQ=255)
                                                                                                                                         gcgACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTAc       <  2:2957859/148‑1 (MQ=255)
                                                                                                                                           gACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATTACGCTTATCATAAAAATGTACCg     <  2:184743/148‑1 (MQ=255)
                                                                                                                                           gACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCg     <  1:3517837/148‑1 (MQ=255)
                                                                                                                                              cactcaGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTgc  <  1:2076970/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTGC  >  CP009273/236089‑236378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑